Abstract:
:The dissemination of extended-spectrum β-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 and pHK01-like plasmids, which belong to incompatibility group FII (IncFII). In this work, we studied the physiological effect caused by the pHK01 plasmid in bacterial host Escherichia coli J53. The plasmid did not affect cell growth of the host but reduced their motility. The reduction of host motility was attributed to downregulation of genes that encode the flagellar system. We also identified several plasmid-encoded sRNAs, and showed that the overexpression of one of them, AS-traI, in the presence of pHK01 plasmid shortened the lag phase of host growth. In addition to the study of pHK01 in bacteria, we also developed a fast and incompatibility group-specific curing method using countertranscribed RNA, which could be of general usage for studying plasmid-host interaction in clinical aspects.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Jiang X,Liu X,Law COK,Wang Y,Lo WU,Weng X,Chan TF,Ho PL,Lau TCKdoi
10.1093/femsec/fix090subject
Has Abstractpub_date
2017-07-01 00:00:00issue
7eissn
0168-6496issn
1574-6941pii
3940222journal_volume
93pub_type
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