Abstract:
:Rhodococcus sp. strain DTB (DSM 44534) was grown on a mixture of (R,R)-, (S,S)- and meso-bis-(1-chloro-2-propyl) ether (BCPE) as the sole source of carbon and energy. During BCPE degradation 1'-chloro-2'-propyl-3-chloro-2-prop-1-enyl-ether (DVE), 1-chloro-2-propanol and chloroacetone intermediates were formed. The BCPE or DVE stereoisomers were metabolized in consecutive order via scission of the ether bond, with discrimination against the (R) configuration. Resting cell suspensions of Rhodococcus pregrown on BCPE showed a preferential attack of the (S)-configured ether-linked carbons, resulting in an enantioselective enrichment of (R,R)-BCPE. Microbial discrimination of BCPE or DVE isomers and chemical conversion of the intermediates to 1-chloro-2-propanol allowed the identification of the configuration of all BCPE isomers and the DVE enantiomers. Elucidation of the absolute configuration of the 1-chloro-2-propanol isomers was achieved by enantioselective chemical synthesis.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Garbe LA,Moreno-Horn M,Tressl R,Görisch Hdoi
10.1111/j.1574-6941.2005.00004.xsubject
Has Abstractpub_date
2006-01-01 00:00:00pages
113-21issue
1eissn
0168-6496issn
1574-6941pii
FEM004journal_volume
55pub_type
杂志文章abstract::Qualitative and quantitative changes of microbial communities in soil microcosms during bioremediation were determined throughout one year. The soil was contaminated with 0%, 2.5%, 5%, 10% (wt/wt) of petrochemical sludge containing polynuclear aromatic hydrocarbons. We analyzed the hydrocarbon concentration in the mic...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/j.femsec.2005.01.014
更新日期:2005-07-01 00:00:00
abstract::The effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulescens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding th...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/j.femsec.2004.02.004
更新日期:2004-05-01 00:00:00
abstract::The advent of next-generation sequencing has allowed huge amounts of DNA sequence data to be produced, advancing the capabilities of microbial ecosystem studies. The current challenge is to identify from which microorganisms and genes the DNA originated. Several tools and databases are available for annotating DNA seq...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiw095
更新日期:2016-07-01 00:00:00
abstract::Abstract The impact of salinity on the composition and functional performance (biomass production, growth efficiency and growth rates) of bacterial communities was investigated using batch cultures growing on dissolved organic carbon from a river draining into the Northern Baltic Sea. The cultures were adjusted to riv...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/S0168-6496(03)00149-1
更新日期:2003-07-01 00:00:00
abstract::Integrating conjugative elements (ICEs) of the SXT/R391 family have been described in Vibrios, mainly Vibrio cholerae, and other bacteria as carriers of variable gene content conferring adaptive advantages upon their hosts, including antimicrobial resistance and motility regulation. However, our knowledge on their hos...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/1574-6941.12007
更新日期:2013-02-01 00:00:00
abstract::This paper demonstrates the first microbiological sampling of the Outokumpu deep borehole (2516 m deep) aiming at characterizing the bacterial community composition and diversity of sulphate-reducing bacteria (SRB) in Finnish crystalline bedrock aquifers. Sampling was performed using a 1500-m-long pressure-tight tube ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01111.x
更新日期:2011-08-01 00:00:00
abstract::EFSA responded to our perspective article on the safe use of the insect pathogen Bacillus thrurigiensis (Bt). In doing so they admitted that there is no direct evidence to suggest that B. thuringiensis can cause diarrhoea. They nevertheless continue to repeat the assertion that Bt cannot be distinguished from Bacillus...
journal_title:FEMS microbiology ecology
pub_type: 评论,信件
doi:10.1093/femsec/fix169
更新日期:2018-01-01 00:00:00
abstract::Selenium is an essential trace element that can modulate the gut microbiome with an impact on host health. The present study aimed to evaluate the effects of organic (selenium-enriched yeast) vs inorganic (sodium selenite) selenium source on fecal end-fermentation products and gut microbiome of puppies from 20 to 52 w...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiaa212
更新日期:2020-10-21 00:00:00
abstract::Although anaerobic biodegradation of di-n-butyl phthalic acid ester (DBP) has been studied over the past decade, only little is known about the microorganisms involved in the biological anaerobic degradation pathways. The aim of this work is to characterize the microbial community dynamics in enrichment cultures degra...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00570.x
更新日期:2008-11-01 00:00:00
abstract::Methane seepages typically harbor communities of anaerobic methane oxidizers (ANME); however, knowledge about fine-scale vertical variation of ANME in response to geochemical gradients is limited. We investigated microbial communities in sediments below a white microbial mat in the G11 pockmark at Nyegga by 16S rRNA g...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2011.01153.x
更新日期:2011-11-01 00:00:00
abstract::A major obstacle in the molecular investigation of natural, especially oceanic, microbial cells is their adequate preservation for further land-based molecular analyses. Here, we examined the use of microwaves for cell fixation before high-speed flow cytometric sorting to define the metaproteomes and metagenomes of ke...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2010.00927.x
更新日期:2010-10-01 00:00:00
abstract::Sediments from six soda lakes of the Kulunda Steppe (Altai, Russia) and from hypersaline alkaline lakes of Wadi Natrun (Egypt) were analyzed for the presence of cbb and aclB genes encoding key enzymes Ci assimilation (RuBisCO in Calvin-Benson and ATP citrate lyase in rTCA cycles, respectively). The cbbL gene (RuBisCO ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2010.00996.x
更新日期:2011-01-01 00:00:00
abstract::In an effort to identify the causes and patterns of temporal change in periphytic communities, we examined biomass accumulation, taxonomic and functional composition, rate of species turnover, and pairwise species correlations in response to variability in current velocity and nutrient supply in artificial stream flum...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01302.x
更新日期:2012-05-01 00:00:00
abstract::Previous microscopy-based studies in the eastern English Channel have revealed it to be a productive meso-eutrophic coastal ecosystem, characterized by strong repeating patterns in microplankton succession. The present study examines the seasonal structure of the entire protistan community from March 2011 to July 2013...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiv034
更新日期:2015-05-01 00:00:00
abstract::Microbial community composition is essential for aquatic ecosystem functions and has been explored across diverse environments and various spatial scales. However, documented patterns are often based on samples from spatially/geographically separated locations or sites. Here, we define sampling volume as spatial scale...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix124
更新日期:2017-10-01 00:00:00
abstract::Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2009.00806.x
更新日期:2010-02-01 00:00:00
abstract::Filamentous Chloroflexi species are often present in activated sludge wastewater treatment plants in relatively low numbers, although bulking incidences caused by Chloroflexi filaments have been observed. A new species-specific gene probe for FISH was designed and using phylum-, subdivision-, morphotype 1851- and spec...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2006.00251.x
更新日期:2007-03-01 00:00:00
abstract::Riverine biofilms remain one of the least-studied habitats despite the significant increase in the examination of aquatic microbial communities in recent years. In this study, the dynamics of epilithic biofilm communities native on rocks from a low-order upland stream were examined over a period of 3 years. Spatial an...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2008.00480.x
更新日期:2008-06-01 00:00:00
abstract::Although microbial communities from many glacial environments have been analyzed, microbes living in the debris atop debris-covered glaciers represent an understudied frontier in the cryosphere. The few previous molecular studies of microbes in supraglacial debris have either had limited phylogenetic resolution, limit...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix095
更新日期:2017-08-01 00:00:00
abstract::While Hymenoscyphus fraxineus causes dieback of the European ash (Fraxinus excelsior), flowering ash (F. ornus) appears resistant to the pathogen. To date, contributions of endophytic fungi to host resistance are unknown. The following hypotheses were tested: (i) endophytic fungi enhance the resistance of F. excelsior...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiw142
更新日期:2016-09-01 00:00:00
abstract::The impact of low pathogenic influenza viruses such as subtype H9N2, which infect the respiratory and the gastrointestinal tracts of chickens, on microbial composition are not known. Twenty-day-old specific pathogen-free chickens were assigned to two treatment groups, control (uninfected) and H9N2-infected (challenged...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fix165
更新日期:2018-01-01 00:00:00
abstract::The objective of the study was to elucidate the depth distribution and community composition of Archaea in a temperate acidic forest soil. Numbers of Archaea and Bacteria were measured in the upper 18 cm of the soil, and soil cores were sampled on two separate occasions using quantitative PCR targeting 16S rRNA genes....
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2007.00310.x
更新日期:2007-06-01 00:00:00
abstract::The polychlorinated biphenyl (PCB)-degrading Pseudomonas sp. B4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. Pseudomonas sp. B4 was attracted to biphenyl, PCBs and benzoate in swarm plate and capillary assays. Chemotaxis towar...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2007.00293.x
更新日期:2007-05-01 00:00:00
abstract::Obligate psychrophilic, facultative psychrophilic, and mesophilic yeasts were cultured in a carbon-rich medium at different temperatures to investigate whether growth parameters, lipid accumulation, and fatty acid (FA) composition were adaptive and/or acclimatory responses. Acclimation of facultative psychrophiles and...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2009.00727.x
更新日期:2009-09-01 00:00:00
abstract::Sulfate-reducing bacteria (SRB) pose a serious problem to offshore oil industries by producing sulfide, which is highly reactive, corrosive and toxic. The dissimilatory sulfite reductase (dsr) gene encodes for enzyme dissimilatory sulfite reductase and catalyzes the conversion of sulfite to sulfide. Because this gene ...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2009.00714.x
更新日期:2009-08-01 00:00:00
abstract::Cetaceans have evolved from herbivorous terrestrial artiodactyls closely related to ruminants and hippopotamuses. Delphinidae, a family included in this order, represent an extreme and successful re-adaptation of mammalian physiology to the marine habitat and piscivorous diet. The anatomical aspects of Delphinidae suc...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiw055
更新日期:2016-04-01 00:00:00
abstract::Five aerobic intestinal bacterial strains that utilized chloramphenicol (CAP) as sole carbon and energy source were isolated from fecal samples collected from healthy volunteers. Based on 16S rRNA gene sequence analysis, four of the five strains were identified as Klebsiella pneumoniae and one as Escherichia fergusoni...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1111/j.1574-6941.2012.01440.x
更新日期:2012-12-01 00:00:00
abstract::Mimosa caesalpiniifolia Benth. is a legume native to the semi-arid region of Brazil, in the Northeast. Its successful adaptation to other locations, such as the Atlantic Forest in the Southeast region, may be related to its ability to establish symbiosis with nitrogen-fixing bacteria, especially β-rhizobia of the genu...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz109
更新日期:2019-08-01 00:00:00
abstract::Microbial communities in two production waters of a low-temperature and low-salinity petroleum reservoir in Canada were examined using cultural and molecular approaches. The predominant cultivated microorganisms were homoacetogens but sulfate-reducers, acetoclastic methanogens and denitrifiers also gave significant co...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1016/j.femsec.2005.05.007
更新日期:2005-11-01 00:00:00
abstract::Determining the factors underlying the assembly, structure, and diversity of symbiont communities remains a focal point of animal-microbiome research. Much of these efforts focus on taxonomic variation of microbiota within or between animal populations, but rarely test the proportional impacts of ecological components...
journal_title:FEMS microbiology ecology
pub_type: 杂志文章
doi:10.1093/femsec/fiz103
更新日期:2019-08-01 00:00:00