Exploring the composition and diversity of microbial communities at the Jan Mayen hydrothermal vent field using RNA and DNA.

Abstract:

:DNA sequencing technology has proven very valuable for analysing the microbiota of poorly accessible ecosystems such as hydrothermal vents. Using a combination of amplicon and shotgun sequencing of small-subunit rRNA and its gene, we examined the composition and diversity of microbial communities from the recently discovered Jan Mayen vent field, located on Mohn's Ridge in the Norwegian-Greenland Sea. The communities were dominated by the epsilonproteobacterial genera Sulfurimonas and Sulfurovum. These are mesophiles involved in sulphur metabolism and typically found in vent fluid mixing zones. Composition and diversity predictions differed systematically between extracted DNA and RNA samples as well as between amplicon and shotgun sequencing. These differences were more substantial than those between two biological replicates. Amplicon vs. shotgun sequencing differences could be explained to a large extent by bias introduced during PCR, caused by preferential primer-template annealing, while DNA vs. RNA differences were thought to be caused by differences between the activity levels of taxa. Further, predicted diversity from RNA samples was consistently lower than that from DNA. In summary, this study illustrates how different methods can provide complementary ecological insights.

journal_name

FEMS Microbiol Ecol

authors

Lanzén A,Jørgensen SL,Bengtsson MM,Jonassen I,Øvreås L,Urich T

doi

10.1111/j.1574-6941.2011.01138.x

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

577-89

issue

3

eissn

0168-6496

issn

1574-6941

journal_volume

77

pub_type

杂志文章
  • Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp.

    abstract::In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeas...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01050.x

    authors: Brilhante RS,Paiva MA,Sampaio CM,Teixeira CE,Castelo-Branco DS,Leite JJ,Moreira CA,Silva LP,Cordeiro RA,Monteiro AJ,Sidrim JJ,Rocha MF

    更新日期:2011-05-01 00:00:00

  • Dynamic changes in the composition of photosynthetic picoeukaryotes in the northwestern Pacific Ocean revealed by high-throughput tag sequencing of plastid 16S rRNA genes.

    abstract::Photosynthetic picoeukaryotes (PPEs) are major oceanic primary producers. However, the diversity of such communities remains poorly understood, especially in the northwestern (NW) Pacific. We investigated the abundance and diversity of PPEs, and recorded environmental variables, along a transect from the coast to the ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv170

    authors: Choi DH,An SM,Chun S,Yang EC,Selph KE,Lee CM,Noh JH

    更新日期:2016-02-01 00:00:00

  • Geographical patterns of root nodule bacterial diversity in cultivated and wild populations of a woody legume crop.

    abstract::There is interest in understanding how cultivation, plant genotype, climate and soil conditions influence the biogeography of root nodule bacterial communities of legumes. For crops from regions with relict wild populations, this is of even greater interest because the effects of cultivation on symbiont communities ca...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa145

    authors: Ramoneda J,Roux JJL,Frossard E,Frey B,Gamper HA

    更新日期:2020-10-01 00:00:00

  • Evaluation of terminal-restriction fragment length polymorphism analysis in contrasting marine environments.

    abstract::Terminal-restriction fragment length polymorphism (T-RFLP) analysis is widely used in microbial ecology studies. In the present study, T-RFLP analysis of PCR products digested by five restriction enzymes (AluI, HaeIII, MspI, Sau3AI and TaqI) was applied for 20 samples from three contrasting coastal environments to ass...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00493.x

    authors: Zhang R,Thiyagarajan V,Qian PY

    更新日期:2008-07-01 00:00:00

  • Temporal shifts of the Norway lobster (Nephrops norvegicus) gut bacterial communities.

    abstract::The aim of this study was to investigate the gut bacterial communities of Nephrops norvegicus individuals, using a suite of molecular tools consisting of automated ribosomal intergenic spacer analysis, 16S rRNA gene-internal transcribed spacer clone libraries and FISH. The animals were collected from Pagasitikos Gulf,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00964.x

    authors: Meziti A,Ramette A,Mente E,Kormas KA

    更新日期:2010-11-01 00:00:00

  • Metronidazole effects on microbiota and mucus layer thickness in the rat gut.

    abstract::Both mucus and mucosa-associated bacteria form a specific environment in the gut; their disruption may play a crucial role in the development of intestinal bowel disease (IBD). Metronidazole, an antibiotic used in the treatment of IBD, alters gut microbiota and reduces basal oxidative stress to proteins in colonic tis...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00916.x

    authors: Pélissier MA,Vasquez N,Balamurugan R,Pereira E,Dossou-Yovo F,Suau A,Pochart P,Magne F

    更新日期:2010-09-01 00:00:00

  • An improved electroelution method for separation of DNA from humic substances in marine sediment DNA extracts.

    abstract::We present a method for the rapid and simple extraction of DNA from marine sediments using electroelution. It effectively separates DNA from compounds, including humic substances, that interfere with subsequent DNA quantification and amplification. After extraction of the DNA from the sediment into an aqueous solution...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00684.x

    authors: Kallmeyer J,Smith DC

    更新日期:2009-07-01 00:00:00

  • Impacts on microbial communities and cultivable isolates from groundwater contaminated with high levels of nitric acid-uranium waste.

    abstract::Microbial communities were characterized at contaminated sites that had elevated levels of nitrate, nickel, aluminum, and uranium (up to 690 mM, 310 microM, 42 mM, and 30 microM, respectively). The bacterial community structure based upon clonal libraries of the SSU rRNA genes (screened clones = 876) was diverse at th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2005.01.010

    authors: Fields MW,Yan T,Rhee SK,Carroll SL,Jardine PM,Watson DB,Criddle CS,Zhou J

    更新日期:2005-08-01 00:00:00

  • Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho.

    abstract::Improving our understanding of antibiotic resistance in soils is important for the protection of human, animal and ecological health. In south-central Idaho, antibiotic resistance genes (ARGs) [blaCTX-M-1, erm(B), sul1, tet(B), tet(M) and tet(X)] and a class 1 integron-integrase gene (intI1) were quantified in agricul...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz071

    authors: Dungan RS,Strausbaugh CA,Leytem AB

    更新日期:2019-06-01 00:00:00

  • High similarity between bacterioneuston and airborne bacterial community compositions in a high mountain lake area.

    abstract::The bacterioneuston (bacteria inhabiting the air-water interface) is poorly characterized and possibly forms a unique community in the aquatic environment. In high mountain lakes, the surface film is subjected to extreme conditions of life, suggesting the development of a specific and adapted bacterioneuston community...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00617.x

    authors: Hervas A,Casamayor EO

    更新日期:2009-02-01 00:00:00

  • Salinity as a structuring factor for the composition and performance of bacterioplankton degrading riverine DOC.

    abstract::Abstract The impact of salinity on the composition and functional performance (biomass production, growth efficiency and growth rates) of bacterial communities was investigated using batch cultures growing on dissolved organic carbon from a river draining into the Northern Baltic Sea. The cultures were adjusted to riv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00149-1

    authors: Langenheder S,Kisand V,Wikner J,Tranvik LJ

    更新日期:2003-07-01 00:00:00

  • An emerging view of the diversity, ecology and function of Archaea in alkaline hydrothermal environments.

    abstract::The described diversity within the domain Archaea has recently expanded due to advances in sequencing technologies, but many habitats that likely harbor novel lineages of archaea remain understudied. Knowledge of archaea within natural and engineered hydrothermal systems, such as hot springs and engineered subsurface ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa246

    authors: Mueller RC,Peach JT,Skorupa DJ,Copié V,Bothner B,Peyton BM

    更新日期:2021-01-26 00:00:00

  • Age-related differences revealed in Australian fur seal Arctocephalus pusillus doriferus gut microbiota.

    abstract::The gut microbiota of Australian fur seals (Arctocephalus pusillus doriferus) was examined at different age classes using fluorescent in situ hybridisation (FISH) and 16S rRNA gene pyrosequencing. The FISH results indicated that in the fur seal groups, the predominant phyla are Firmicutes (22.14-67.33%) followed by Ba...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12157

    authors: Smith SC,Chalker A,Dewar ML,Arnould JP

    更新日期:2013-11-01 00:00:00

  • Distinct composition signatures of archaeal and bacterial phylotypes in the Wanda Glacier forefield, Antarctic Peninsula.

    abstract::Several studies have shown that microbial communities in Antarctic environments are highly diverse. However, considering that the Antarctic Peninsula is among the regions with the fastest warming rates, and that regional climate change has been linked to an increase in the mean rate of glacier retreat, the microbial d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiu005

    authors: Pessi IS,Osorio-Forero C,Gálvez EJ,Simões FL,Simões JC,Junca H,Macedo AJ

    更新日期:2015-01-01 00:00:00

  • Aspect has a greater impact on alpine soil bacterial community structure than elevation.

    abstract::Gradients in environmental conditions, including climate factors and resource availability, occur along mountain inclines, providing a 'natural laboratory' to explore their combined impacts on microbial distributions. Conflicting spatial patterns observed across elevation gradients in soil bacterial community structur...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw253

    authors: Wu J,Anderson BJ,Buckley HL,Lewis G,Lear G

    更新日期:2017-03-01 00:00:00

  • Survival of potentially pathogenic human-associated bacteria in the rhizosphere of hydroponically grown wheat.

    abstract::Plants may serve as reservoirs for human-associated bacteria (H-AB) in long-term space missions containing bioregenerative life support systems. The current study examined the abilities of five human-associated potential pathogens, Pseudomonas aeruginosa, Pseudomonas cepacia, Staphylococcus aureus, Streptococcus pyoge...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/0168-6496(96)00020-7

    authors: Morales A,Garland JL,Lim DV

    更新日期:1996-07-01 00:00:00

  • Fecal bacterial communities of wild-captured and stranded green turtles (Chelonia mydas) on the Great Barrier Reef.

    abstract::Green turtles (Chelonia mydas) are endangered marine herbivores that break down food particles, primarily sea grasses, through microbial fermentation. However, the microbial community and its role in health and disease is still largely unexplored. In this study, we investigated and compared the fecal bacterial communi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix139

    authors: Ahasan MS,Waltzek TB,Huerlimann R,Ariel E

    更新日期:2017-12-01 00:00:00

  • Change of bacterial communities in sediments along Songhua River in Northeastern China after a nitrobenzene pollution event.

    abstract::More than 100 tons of nitrobenzene and related compounds were released into Songhua River due to the explosion of an aniline production factory in November, 2005. Sediment samples were taken from the heavily polluted drainage canal, one upstream and three downstream river sites. The change of bacterial community struc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00540.x

    authors: Li D,Yang M,Li Z,Qi R,He J,Liu H

    更新日期:2008-09-01 00:00:00

  • Effect of temperature change on the composition of the bacterial and archaeal community potentially involved in the turnover of acetate and propionate in methanogenic rice field soil.

    abstract::The microbial community structure was investigated together with the path of methane production in Italian rice field soil incubated at moderate (35 degrees C) and high (45 degrees C) temperature using terminal restriction fragment length polymorphism and stable isotope fractionation. The structure of both the archaea...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00883.x

    authors: Noll M,Klose M,Conrad R

    更新日期:2010-08-01 00:00:00

  • A cold-loving crenarchaeon is a substantial part of a novel microbial community in cold sulphidic marsh water.

    abstract::In this paper, we report the identification and first characterization of a novel, cold-loving, prokaryotic community thriving among white-greenish 'streamers' in the cold (c. 10 degrees C) sulphurous water of the marsh Sippenauer Moor near Regensburg, Bavaria, Germany. It consists of the bacterial genus Thiothrix, th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00088.x

    authors: Koch M,Rudolph C,Moissl C,Huber R

    更新日期:2006-07-01 00:00:00

  • Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater.

    abstract::Autotrophic ammonia-oxidising bacteria (AOB) are a crucial component of the microbial communities of nitrifying wastewater treatment systems. Nitrification is known to occur in reactors of different configuration, but whether AOB communities are different in reactors of different design is unknown. We compared the div...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2003.tb01059.x

    authors: Rowan AK,Snape JR,Fearnside D,Barer MR,Curtis TP,Head IM

    更新日期:2003-03-01 00:00:00

  • Characterization of methanogenic Archaea in the leachate of a closed municipal solid waste landfill.

    abstract::Cultivation-independent molecular approaches were used to investigate the phylogenetic composition of Archaea and the relative abundance of phylogenetically defined groups of methanogens in the leachate of a closed municipal solid waste landfill. Cloning and phylogenetic analysis of archaeal 16S rRNA gene sequences (1...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00218-6

    authors: Huang LN,Chen YQ,Zhou H,Luo S,Lan CY,Qu LH

    更新日期:2003-11-01 00:00:00

  • Aerobic anoxygenic phototrophs are highly abundant in hypertrophic and polyhumic waters.

    abstract::Aerobic anoxygenic phototrophs (AAPs) are a group of photoheterotrophic bacteria common in natural waters. Here, AAP abundance and contribution to total bacterial abundance and biomass were investigated to test whether the trophic status of a lake or content of coloured dissolved organic matter (CDOM) play a role in d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz104

    authors: Szabó-Tugyi N,Vörös L,V-Balogh K,Botta-Dukát Z,Bernát G,Schmera D,Somogyi B

    更新日期:2019-08-01 00:00:00

  • Estimation of long-term bacterial respiration and growth efficiency in Lake Kinneret.

    abstract::Semi-annual averaged values of photosynthetic carbon fixation (PCF), community respiration (CR), bacterial productivity (BP) and zooplankton carbon biomass, measured biweekly or monthly, were used to obtain long-term estimates of bacterial respiration (BR) and bacterial growth efficiency (BGE) in Lake Kinneret from 20...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00822.x

    authors: Berman T,Yacobi YZ,Parparov A,Gal G

    更新日期:2010-03-01 00:00:00

  • Analysis of DGGE profiles to explore the relationship between prokaryotic community composition and biogeochemical processes in deep subseafloor sediments from the Peru Margin.

    abstract::The aim of this work was to relate depth profiles of prokaryotic community composition with geochemical processes in the deep subseafloor biosphere at two shallow-water sites on the Peru Margin in the Pacific Ocean (ODP Leg 201, sites 1228 and 1229). Principal component analysis of denaturing gradient gel electrophore...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00144.x

    authors: Fry JC,Webster G,Cragg BA,Weightman AJ,Parkes RJ

    更新日期:2006-10-01 00:00:00

  • Evaluation of PCR primers for universal nifH gene targeting and for assessment of transcribed nifH pools in roots of Oryza longistaminata with and without low nitrogen input.

    abstract::The bias of widely used degenerate nifH-specific primer sets was first tested using denaturing gradient gel electrophoresis (DGGE), and their application for profiling of complex communities assessed for roots of Oryza longistaminata. When primers (P) with mismatches at nondegenerate positions were used on genomic DNA...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00545.x

    authors: Demba Diallo M,Reinhold-Hurek B,Hurek T

    更新日期:2008-08-01 00:00:00

  • Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community.

    abstract::Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00806.x

    authors: Durand L,Zbinden M,Cueff-Gauchard V,Duperron S,Roussel EG,Shillito B,Cambon-Bonavita MA

    更新日期:2010-02-01 00:00:00

  • Microbial indicators are better predictors of wheat yield and quality than N fertilization.

    abstract::In view of their key roles in many soil- and plant-related processes, we hypothesized that soil microorganisms could play a larger role in determining wheat baking quality than nitrogen fertilization. A field experiment was conducted under bread wheat production conditions, where different fertilization treatments, ra...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz205

    authors: Yergeau É,Quiza L,Tremblay J

    更新日期:2020-02-01 00:00:00

  • Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies.

    abstract::Selenium is an essential trace element that can modulate the gut microbiome with an impact on host health. The present study aimed to evaluate the effects of organic (selenium-enriched yeast) vs inorganic (sodium selenite) selenium source on fecal end-fermentation products and gut microbiome of puppies from 20 to 52 w...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa212

    authors: Pereira AM,Pinna C,Biagi G,Stefanelli C,Maia MRG,Matos E,Segundo MA,Fonseca AJM,Cabrita ARJ

    更新日期:2020-10-21 00:00:00

  • Unveiling the fungal mycobiota present throughout the cork stopper manufacturing process.

    abstract::A particular fungal population is present in the main stages of the manufacturing process of cork discs. Its diversity was studied using both dependent (isolation) and independent culture methods (denaturing gel gradient electrophoresis and cloning of the ITS1-5.8S-ITS2 region). The mycobiota in the samples taken in t...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2012.01419.x

    authors: Barreto MC,Houbraken J,Samson RA,Brito D,Gadanho M,San Romão MV

    更新日期:2012-10-01 00:00:00