Antibiotic resistance profiles of Pseudomonas aeruginosa isolated from various Greek aquatic environments.

Abstract:

:A large number of antibiotic-resistant P. aeruginosa isolates are continuously discharged into natural water basins mainly through sewage. However, the environmental reservoirs of antibiotic resistance factors are poorly understood. In this study, the antibiotic resistance patterns of 245 isolates from various aquatic sites in Greece were analysed. Twenty-three isolates with resistance patterns cefotaxime-aztreonam-ceftazidime, cefotaxime-aztreonam-meropenem, cefotaxime-ceftazidime-meropenem, cefotaxime-ceftazidime-aztreonam-meropenem and cefotaxime-ceftazidime-cefepime-aztreonam-meropenem were screened phenotypically for the presence of extended spectrum β-lactamases (ESBLs), while 77 isolates with various resistant phenotypes were screened for the presence of class 1 and class 2 integrase genes. The aztreonam-resistant isolates and ESBL producers were the main resistant phenotypes in all habitats tested. In 13/77 isolates class 1 integron was detected, while all tested isolates were negative for the presence of the class 2 integrase gene. CTX-M group 9 β-lactamase was present in a small number of isolates (three isolates) highlighting the emergence of ESBL genes in aquatic environments. As a conclusion, it seems that Greek water bodies could serve as a potential reservoir of resistant P. aeruginosa isolates posing threats to human and animal health.

journal_name

FEMS Microbiol Ecol

authors

Olga P,Apostolos V,Alexis G,George V,Athena M

doi

10.1093/femsec/fiw042

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

fiw042

issue

5

eissn

0168-6496

issn

1574-6941

pii

fiw042

journal_volume

92

pub_type

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