Transcriptional response of Desulfatibacillum alkenivorans AK-01 to growth on alkanes: insights from RT-qPCR and microarray analyses.

Abstract:

:Microbial transformation of n-alkanes in anaerobic ecosystems plays a pivotal role in biogeochemical carbon cycling and bioremediation, but the requisite genetic machinery is not well elucidated.Desulfatibacillum alkenivorans AK-01 utilizes n-alkanes (C13 to C18) and contains two genomic loci encoding alkylsuccinate synthase (ASS) gene clusters. ASS catalyzes alkane addition to fumarate to form methylalkylsuccinic acids. We hypothesized that the genes in the two clusters would be differentially expressed depending on the alkane substrate utilized for growth. RT-qPCR was used to investigate ass-gene expression across AK-01's known substrate range, and microarray-based transcriptomic analysis served to investigate whole-cell responses to growth on n-hexadecane versus hexadecanoate. RT-qPCR revealed induction of ass gene cluster 1 during growth on all tested alkane substrates, and the transcriptional start sites in cluster 1 were determined via 5'RACE. Induction of ass gene cluster 2 was not observed under the tested conditions. Transcriptomic analysis indicated that the upregulation of genes potentially involved in methylalkylsuccinate metabolism, including methylmalonyl-CoA mutase and a putative carboxyl transferase. These findings provide new directions for studying the transcriptional regulation of genes involved in alkane addition to fumarate, fumarate recycling and the processing of methylalkylsuccinates with regard to isolates, enrichment cultures and ecological datasets.

journal_name

FEMS Microbiol Ecol

authors

Herath A,Wawrik B,Qin Y,Zhou J,Callaghan AV

doi

10.1093/femsec/fiw062

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

fiw062

issue

5

eissn

0168-6496

issn

1574-6941

pii

fiw062

journal_volume

92

pub_type

杂志文章
  • Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground.

    abstract::Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are currently considered a hot spot in fungal biology due to their ecological and economic relevance. Among all the species, Tuber magnatum is the most appreciated because of its special taste and aroma. The aim of this work was to set up...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00783.x

    authors: Zampieri E,Murat C,Cagnasso M,Bonfante P,Mello A

    更新日期:2010-01-01 00:00:00

  • Supplemental selenium source on gut health: insights on fecal microbiome and fermentation products of growing puppies.

    abstract::Selenium is an essential trace element that can modulate the gut microbiome with an impact on host health. The present study aimed to evaluate the effects of organic (selenium-enriched yeast) vs inorganic (sodium selenite) selenium source on fecal end-fermentation products and gut microbiome of puppies from 20 to 52 w...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa212

    authors: Pereira AM,Pinna C,Biagi G,Stefanelli C,Maia MRG,Matos E,Segundo MA,Fonseca AJM,Cabrita ARJ

    更新日期:2020-10-21 00:00:00

  • Community structure of photosynthetic picoeukaryotes differs in lakes with different trophic statuses along the middle-lower reaches of the Yangtze River.

    abstract::Photosynthetic picoeukaryotes (PPEs) play an important role in aquatic ecosystem functioning. There is still a relative lack of information on freshwater PPEs, especially in eutrophic lakes. We used a combination of flow cytometric sorting and pyrosequencing to investigate the PPEs community structure in more than 20 ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy011

    authors: Shi X,Li S,Liu C,Zhang M,Liu M

    更新日期:2018-04-01 00:00:00

  • Molecular bacterial community analysis of clean rooms where spacecraft are assembled.

    abstract::Molecular bacterial community composition was characterized from three geographically distinct spacecraft-associated clean rooms to determine whether such populations are influenced by the surrounding environment or the maintenance of the clean rooms. Samples were collected from facilities at the Jet Propulsion Labora...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2007.00360.x

    authors: Moissl C,Osman S,La Duc MT,Dekas A,Brodie E,DeSantis T,Venkateswaran K

    更新日期:2007-09-01 00:00:00

  • High similarity between bacterioneuston and airborne bacterial community compositions in a high mountain lake area.

    abstract::The bacterioneuston (bacteria inhabiting the air-water interface) is poorly characterized and possibly forms a unique community in the aquatic environment. In high mountain lakes, the surface film is subjected to extreme conditions of life, suggesting the development of a specific and adapted bacterioneuston community...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00617.x

    authors: Hervas A,Casamayor EO

    更新日期:2009-02-01 00:00:00

  • Enrichment and isolation of acid-tolerant sulfate-reducing microorganisms in the anoxic, acidic hot spring sediments from Copahue volcano, Argentina.

    abstract::The geothermal Copahue-Caviahue (GCC) system (Argentina) is an extreme acidic environment, dominated by the activity of Copahue volcano. Environments characterised by low pH values, such as volcanic areas, are of particular interest for the search of acidophilic microorganisms with application in biotechnological proc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz175

    authors: Willis G,Nancucheo I,Hedrich S,Giaveno A,Donati E,Johnson DB

    更新日期:2019-12-01 00:00:00

  • The effect of soil acidification on atmospheric methane uptake by a Maine forest soil(1).

    abstract::Soil from the zone of maximal methanotrophic activity (approximately 5-8 cm depth) in a mixed coniferous-hardwood forest consumed atmospheric methane over a wide pH range (3.5-7.5) with a broad optimum between 4.8 and 6.0. Methane uptake at native soil pH values (4.4-4.8) was only slightly less rapid than rates at opt...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00771.x

    authors: Benstead J,King GM

    更新日期:2001-01-01 00:00:00

  • Mitochondrial sequences of Rhipicephalus and Coxiella endosymbiont reveal evidence of lineages co-cladogenesis.

    abstract::Rhipicephalus ticks are competent vectors of several pathogens, such as Spotted Fever Group Rickettsiae (SFGR) and many Babesia species. Within this genus, different R. sanguineus s.l. lineages show an unequal vector competence and resistance regarding some pathogenic strains. Current literature supports that tick end...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa072

    authors: Coimbra-Dores MJ,Jaarsma RI,Carmo AO,Maia-Silva M,Fonville M,da Costa DFF,Brandão RML,Azevedo F,Casero M,Oliveira AC,Afonso SMS,Sprong H,Rosa F,Dias D

    更新日期:2020-06-01 00:00:00

  • The green macroalga Dictyosphaeria ocellata influences the structure of the bacterioplankton community through differential effects on individual bacterial phylotypes.

    abstract::Marine macroalgae are subjected to large numbers of bacteria in their environment. These bacteria have the potential to affect the health and ecology of algae in a variety of ways and can be both beneficial and harmful to the algae. Therefore, algae have likely evolved mechanisms to differentially regulate the growth ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.01005.x

    authors: Sneed JM,Pohnert G

    更新日期:2011-02-01 00:00:00

  • Seasonality in bacterial diversity in north-west Mediterranean coastal waters: assessment through clone libraries, fingerprinting and FISH.

    abstract::We combined denaturing gradient gel electrophoresis (DGGE), catalysed reporter deposition-FISH (CARD-FISH) and clone libraries to investigate the seasonality of the bacterial assemblage composition in north-west Mediterranean coastal waters. DGGE analysis indicated that bacterial diversity changed gradually throughout...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00276.x

    authors: Alonso-Sáez L,Balagué V,Sà EL,Sánchez O,González JM,Pinhassi J,Massana R,Pernthaler J,Pedrós-Alió C,Gasol JM

    更新日期:2007-04-01 00:00:00

  • Members of Microvirga and Bradyrhizobium genera are native endosymbiotic bacteria nodulating Lupinus luteus in Northern Tunisian soils.

    abstract::The genetic diversity of bacterial populations nodulating Lupinus luteus (yellow lupine) in Northern Tunisia was examined. Phylogenetic analyses of 43 isolates based on recA and gyrB partial sequences grouped them in three clusters, two of which belong to genus Bradyrhizobium (41 isolates) and one, remarkably, to Micr...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix068

    authors: Msaddak A,Rejili M,Durán D,Rey L,Imperial J,Palacios JM,Ruiz-Argüeso T,Mars M

    更新日期:2017-06-01 00:00:00

  • Comparison of the molecular diversity of the methanogenic community at the brackish and marine ends of a UK estuary.

    abstract::Abstract The 16S rRNA sequence diversity of the euryarchaeal community in a predominately freshwater sediment at East Hill Bridge (EHB) on the River Colne estuary, Essex, UK was investigated and compared to that from marine sediments at the mouth of the river (Colne Point). The East Hill Bridge sediments appear to sup...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2002.tb00902.x

    authors: Purdy KJ,Munson MA,Nedwell DB,Martin Embley T

    更新日期:2002-01-01 00:00:00

  • Microbial survey of the mummies from the Capuchin Catacombs of Palermo, Italy: biodeterioration risk and contamination of the indoor air.

    abstract::The Capuchin Catacombs of Palermo contain over 1800 preserved bodies dating from the 16th to 20th centuries AD and showing evidence of biodeterioration. An extensive microbiological and molecular investigation was recently performed. Samples were taken from skin, muscle, hair, bone, stuffing materials, clothes, and su...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12165

    authors: Piñar G,Piombino-Mascali D,Maixner F,Zink A,Sterflinger K

    更新日期:2013-11-01 00:00:00

  • Distinct commensal bacteria associated with ingesta and mucosal epithelium in the gastrointestinal tracts of calves and chickens.

    abstract::The primary aim of this study was to determine whether distinct gastrointestinal tract (GIT) microbial communities are established within ingesta and on mucosal surfaces of dairy calves and chickens to evaluate whether the principle of microbial segregation is of broad biological significance. Multivariate analysis of...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01220.x

    authors: Malmuthuge N,Li M,Chen Y,Fries P,Griebel PJ,Baurhoo B,Zhao X,Guan LL

    更新日期:2012-02-01 00:00:00

  • Abundance and activity of nitrate reducers in an arable soil are more affected by temporal variation and soil depth than by elevated atmospheric [CO2].

    abstract::Elevated atmospheric carbon dioxide concentrations ([CO(2) ]) might change the abundance and the function of soil microorganisms in the depth profile of agricultural soils by plant-mediated reactions. The seasonal pattern of abundance and activity of nitrate-reducing bacteria was studied in a Mini-FACE experiment plan...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01048.x

    authors: Marhan S,Philippot L,Bru D,Rudolph S,Franzaring J,Högy P,Fangmeier A,Kandeler E

    更新日期:2011-05-01 00:00:00

  • Dryland soil microbial communities display spatial biogeographic patterns associated with soil depth and soil parent material.

    abstract::Biological soil crusts (biocrusts) are common to drylands worldwide. We employed replicated, spatially nested sampling and 16S rRNA gene sequencing to describe the soil microbial communities in three soils derived from different parent material (sandstone, shale, and gypsum). For each soil type, two depths (biocrusts,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12143

    authors: Steven B,Gallegos-Graves LV,Belnap J,Kuske CR

    更新日期:2013-10-01 00:00:00

  • An improved electroelution method for separation of DNA from humic substances in marine sediment DNA extracts.

    abstract::We present a method for the rapid and simple extraction of DNA from marine sediments using electroelution. It effectively separates DNA from compounds, including humic substances, that interfere with subsequent DNA quantification and amplification. After extraction of the DNA from the sediment into an aqueous solution...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00684.x

    authors: Kallmeyer J,Smith DC

    更新日期:2009-07-01 00:00:00

  • Harnessing long-read amplicon sequencing to uncover NRPS and Type I PKS gene sequence diversity in polar desert soils.

    abstract::The severity of environmental conditions at Earth's frigid zones present attractive opportunities for microbial biomining due to their heightened potential as reservoirs for novel secondary metabolites. Arid soil microbiomes within the Antarctic and Arctic circles are remarkably rich in Actinobacteria and Proteobacter...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz031

    authors: Benaud N,Zhang E,van Dorst J,Brown MV,Kalaitzis JA,Neilan BA,Ferrari BC

    更新日期:2019-04-01 00:00:00

  • Microbiomes inhabiting rice roots and rhizosphere.

    abstract::Land plants directly contact soil through their roots. An enormous diversity of microbes dwelling in root-associated zones, including endosphere (inside root), rhizoplane (root surface) and rhizosphere (soil surrounding the root surface), play essential roles in ecosystem functioning and plant health. Rice is a staple...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz040

    authors: Ding LJ,Cui HL,Nie SA,Long XE,Duan GL,Zhu YG

    更新日期:2019-05-01 00:00:00

  • Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna.

    abstract::Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00881.x

    authors: Burgaud G,Arzur D,Durand L,Cambon-Bonavita MA,Barbier G

    更新日期:2010-07-01 00:00:00

  • Ectomycorrhizal hyphae structure components of the soil bacterial community for decreased phosphatase production.

    abstract::Ectomycorrhizal fungi (EMF) provide nutrients to their hosts by means of hyphae that extend beyond nutrient-depleted rhizosphere soil. Soil bacteria may compete with EMF for nutrients or may act synergistically to enhance nutrient supply to hosts. To assess the interactions between hyphae and bacteria, two types of sm...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01060.x

    authors: Brooks DD,Chan R,Starks ER,Grayston SJ,Jones MD

    更新日期:2011-05-01 00:00:00

  • Effect of temperature change on the composition of the bacterial and archaeal community potentially involved in the turnover of acetate and propionate in methanogenic rice field soil.

    abstract::The microbial community structure was investigated together with the path of methane production in Italian rice field soil incubated at moderate (35 degrees C) and high (45 degrees C) temperature using terminal restriction fragment length polymorphism and stable isotope fractionation. The structure of both the archaea...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00883.x

    authors: Noll M,Klose M,Conrad R

    更新日期:2010-08-01 00:00:00

  • A bacterial culture-independent method to investigate chemically mediated control of bacterial epibiosis in marine invertebrates by using TRFLP analysis and natural bacterial populations.

    abstract::It has been postulated that a variety of physically undefended marine invertebrates have evolved strategies to control microbial epibiosis chemically. Ecologically meaningful experiments that demonstrate chemically mediated antibacterial effects are difficult due to the small number of cultivable bacteria. Based on th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00246-0

    authors: Harder T,Lau SC,Tam WY,Qian PY

    更新日期:2004-01-01 00:00:00

  • Long-term antibiotic exposure in soil is associated with changes in microbial community structure and prevalence of class 1 integrons.

    abstract::Antimicrobial resistance is one of the most significant challenges facing the global medical community and can be attributed to the use and misuse of antibiotics. This includes use as growth promoters or for prophylaxis and treatment of bacterial infection in intensively farmed livestock from where antibiotics can ent...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw159

    authors: Cleary DW,Bishop AH,Zhang L,Topp E,Wellington EM,Gaze WH

    更新日期:2016-10-01 00:00:00

  • Potential for atmospheric deposition of bacteria to influence bacterioplankton communities.

    abstract::Biogeographic patterns in microbial communities are an exciting but controversial topic in microbial ecology. Advances in theory pertaining to assembly of microbial communities have made strong assumptions about dispersal of bacteria without exploration. For this reason, we investigated rates of atmospheric bacterial ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00476.x

    authors: Jones SE,Newton RJ,McMahon KD

    更新日期:2008-06-01 00:00:00

  • Activity, structure and dynamics of the methanogenic archaeal community in a flooded Italian rice field.

    abstract::Methane production was studied in an Italian rice field over two consecutive years (1998, 1999) by measuring the rates of total and acetate-dependent methanogenesis in soil and root samples. Population dynamics of methanogens were followed by terminal restriction fragment length polymorphism and real-time PCR targetin...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.09.004

    authors: Krüger M,Frenzel P,Kemnitz D,Conrad R

    更新日期:2005-02-01 00:00:00

  • Prokaryotic community structure and diversity in the sediments of an active submarine mud volcano (Kazan mud volcano, East Mediterranean Sea).

    abstract::We investigated 16S rRNA gene diversity at a high sediment depth resolution (every 5 cm, top 30 cm) in an active site of the Kazan mud volcano, East Mediterranean Sea. A total of 242 archaeal and 374 bacterial clones were analysed, which were attributed to 38 and 205 unique phylotypes, respectively (> or = 98% similar...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00857.x

    authors: Pachiadaki MG,Lykousis V,Stefanou EG,Kormas KA

    更新日期:2010-06-01 00:00:00

  • Siderophore-mediated cooperation and virulence in Pseudomonas aeruginosa.

    abstract::Why should organisms cooperate with each other? Helping close relatives that are likely to share the same genes (kin selection) is one important explanation that is likely to apply across taxa. The production of metabolically costly extracellular iron-scavenging molecules (siderophores) by microorganisms is a cooperat...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6941.2007.00388.x

    authors: Buckling A,Harrison F,Vos M,Brockhurst MA,Gardner A,West SA,Griffin A

    更新日期:2007-11-01 00:00:00

  • Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR.

    abstract::Sulfate-reducing bacteria (SRB) pose a serious problem to offshore oil industries by producing sulfide, which is highly reactive, corrosive and toxic. The dissimilatory sulfite reductase (dsr) gene encodes for enzyme dissimilatory sulfite reductase and catalyzes the conversion of sulfite to sulfide. Because this gene ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00714.x

    authors: Agrawal A,Lal B

    更新日期:2009-08-01 00:00:00

  • The role of motility in the in vitro attachment of Pseudomonas putida PaW8 to wheat roots.

    abstract::The attachment of motile and non-motile strains of Pseudomonas putida PaW8 to sterile wheat roots was assessed in both non-competitive and intra-specific competitive assays. The motile strain showed significantly greater attachment to wheat roots than non-motile strains in phosphate buffer. Overall, the motile strain ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00788.x

    authors: Turnbull GA,Morgan JA,Whipps JM,Saunders JR

    更新日期:2001-03-01 00:00:00