Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages.

Abstract:

:The second step of nitrification, the oxidation of nitrite to nitrate, is vital for the functioning of the nitrogen cycle, but our understanding of the ecological roles of the involved microorganisms is still limited. The known diversity of Nitrospira, the most widely distributed nitrite-oxidizing bacteria, has increased remarkably by analyses of 16S rRNA and functional gene sequences. However, only few representatives could be brought into laboratory cultures so far. In this study, two Nitrospira from activated sludge were isolated using novel approaches together with established methods. Highly enriched 'Candidatus Nitrospira defluvii' was separated from concomitant heterotrophs by taking advantage of its resistance against ampicillin and acriflavine. Beside this member of lineage I, a novel species of lineage II, named N. lenta, was initially enriched at 10°C and finally purified by using optical tweezers. The tolerance to elevated nitrite levels was much higher in N. defluvii than in the more fastidious N. lenta and was accompanied by pronounced biofilm formation. Phylogenetic classification of 12 additional enrichments indicated that Nitrospira lineage I is common in extreme and moderate ecosystems like lineage II. The new cultures will help to explore physiological and genomic differences affecting niche separation between members of this highly diverse genus.

journal_name

FEMS Microbiol Ecol

authors

Nowka B,Off S,Daims H,Spieck E

doi

10.1093/femsec/fiu031

subject

Has Abstract

pub_date

2015-03-01 00:00:00

issue

3

eissn

0168-6496

issn

1574-6941

pii

fiu031

journal_volume

91

pub_type

杂志文章
  • Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho.

    abstract::Improving our understanding of antibiotic resistance in soils is important for the protection of human, animal and ecological health. In south-central Idaho, antibiotic resistance genes (ARGs) [blaCTX-M-1, erm(B), sul1, tet(B), tet(M) and tet(X)] and a class 1 integron-integrase gene (intI1) were quantified in agricul...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz071

    authors: Dungan RS,Strausbaugh CA,Leytem AB

    更新日期:2019-06-01 00:00:00

  • Truffle brûlé: an efficient fungal life strategy.

    abstract::The terms 'brûlé' and 'burnt' are used to describe vegetation-devoid areas of the ground around a range of woody plants interacting with certain truffle species. Increasing interest is currently focused on a systematic search for and study of volatile organic compounds (VOCs) emitted by truffles in the course of their...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6941.2011.01283.x

    authors: Streiblová E,Gryndlerová H,Gryndler M

    更新日期:2012-04-01 00:00:00

  • Modification of Escherichia coli-bacteriophage interactions by surfactants and antibiotics in vitro.

    abstract::Although experiments indicate that the abiotic environment plays an important role in bacterial interactions with their parasitic viruses (bacteriophages or phages), it is not yet clear how exposure to compounds present in nature alters the impact of phages on bacterial growth and evolution. To address this question, ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw211

    authors: Scanlan PD,Bischofberger AM,Hall AR

    更新日期:2017-01-01 00:00:00

  • Biofilm growth and control in cooling water industrial systems.

    abstract::Matrix-embedded, surface-attached microbial communities, known as biofilms, profusely colonise industrial cooling water systems, where the availability of nutrients and organic matter favours rapid microbial proliferation and their adhesion to surfaces in the evaporative fill material, heat exchangers, water reservoir...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1093/femsec/fiy044

    authors: Di Pippo F,Di Gregorio L,Congestri R,Tandoi V,Rossetti S

    更新日期:2018-05-01 00:00:00

  • Effects of endophytic fungi on the ash dieback pathogen.

    abstract::While Hymenoscyphus fraxineus causes dieback of the European ash (Fraxinus excelsior), flowering ash (F. ornus) appears resistant to the pathogen. To date, contributions of endophytic fungi to host resistance are unknown. The following hypotheses were tested: (i) endophytic fungi enhance the resistance of F. excelsior...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw142

    authors: Schlegel M,Dubach V,von Buol L,Sieber TN

    更新日期:2016-09-01 00:00:00

  • Use of 16S rRNA-targeted oligonucleotide probes to investigate the distribution of sulphate-reducing bacteria in estuarine sediments.

    abstract::The distribution of sulphate-reducing bacteria (SRBs) in three anaerobic sediments, one predominantly freshwater and low sulphate and two predominantly marine and high sulphate, on the River Tama, Tokyo, Japan, was investigated using 16S rRNA-targeted oligonucleotide probes. Hybridisation results and sulphate reductio...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00836.x

    authors: Purdy KJ,Nedwell DB,Embley TM,Takii S

    更新日期:2001-07-01 00:00:00

  • Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR.

    abstract::Sulfate-reducing bacteria (SRB) pose a serious problem to offshore oil industries by producing sulfide, which is highly reactive, corrosive and toxic. The dissimilatory sulfite reductase (dsr) gene encodes for enzyme dissimilatory sulfite reductase and catalyzes the conversion of sulfite to sulfide. Because this gene ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00714.x

    authors: Agrawal A,Lal B

    更新日期:2009-08-01 00:00:00

  • Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China.

    abstract::A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection m...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01198.x

    authors: Li L,Sinkko H,Montonen L,Wei G,Lindström K,Räsänen LA

    更新日期:2012-01-01 00:00:00

  • Molecular characterization of plasmid-mediated AmpC beta-lactamase- and extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae among corvids (Corvus brachyrhynchos and Corvus corax) roosting in Canada.

    abstract::This study evaluated the carriage of AmpC and extended-spectrum beta-lactamase (ESBL) genes and associated plasmids in faecal bacteria of Canadian corvids. Faecal samples from 449 birds in five roosting sites across Canada were analyzed using selective media, screening for AmpC and ESBL genes by PCR, and sequencing. G...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiy166

    authors: Jamborova I,Janecko N,Halova D,Sedmik J,Mezerova K,Papousek I,Kutilova I,Dolejska M,Cizek A,Literak I

    更新日期:2018-11-01 00:00:00

  • Non-Redfield, nutrient synergy and flexible internal elemental stoichiometry in a marine bacterium.

    abstract::The stoichiometric constraints of algal growth are well understood, whereas there is less knowledge for heterotrophic bacterioplankton. Growth of the marine bacterium Phaeobacter inhibens DSM 17395, belonging to the globally distributed Roseobacter group, was studied across a wide concentration range of NH4+ and PO43-...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix059

    authors: Trautwein K,Feenders C,Hulsch R,Ruppersberg HS,Strijkstra A,Kant M,Vagts J,Wünsch D,Michalke B,Maczka M,Schulz S,Hillebrand H,Blasius B,Rabus R

    更新日期:2017-05-01 00:00:00

  • Adaptation of ammonia-oxidizing microorganisms to environment shift of paddy field soil.

    abstract::Adaptation of microorganisms to the environment is a central theme in microbial ecology. The objective of this study was to investigate the response of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) to a soil medium shift. We employed two rice field soils collected from Beijing and Hangzhou, Chin...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01271.x

    authors: Ke X,Lu Y

    更新日期:2012-04-01 00:00:00

  • Drivers of epsilonproteobacterial community composition in sulfidic caves and springs.

    abstract::Epsilonproteobacteria are widely distributed in marine, freshwater, and terrestrial environments, although most well-studied groups are from hydrothermal vents and the human intestinal tract. The environmental variables that control epsilonproteobacterial communities in sulfidic terrestrial environments, however, are ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01231.x

    authors: Rossmassler K,Engel AS,Twing KI,Hanson TE,Campbell BJ

    更新日期:2012-02-01 00:00:00

  • Persistence of DNA of Gaeumannomyces graminis var. tritici in soil as measured by a DNA-based assay.

    abstract::There are an increasing number of assays available for fungal plant pathogens based on DNA technology. We have developed such an assay for Gaeumannomyces graminis var. tritici (Ggt) in soil, using slot-blot hybridisation. To ensure the validity of DNA-based soil assays for the fungus, it is important to determine the ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00255-1

    authors: Herdina,Neate S,Jabaji-Hare S,Ophel-Keller K

    更新日期:2004-02-01 00:00:00

  • Isolation of new fastidious alpha Proteobacteria and Afipia felis from hospital water supplies by direct plating and amoebal co-culture procedures.

    abstract::As water is a source of nosocomial infections in hospitals, the presence of fastidious Gram-negative bacteria in water samples taken in a university hospital was investigated. Water samples were inoculated onto agar plates and into amoebal microplates for co-culture. Sixty-eight alpha proteobacteria isolates were obta...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/s0168-6496(00)00084-2

    authors: La Scola B,Barrassi L,Raoult D

    更新日期:2000-12-01 00:00:00

  • Metronidazole effects on microbiota and mucus layer thickness in the rat gut.

    abstract::Both mucus and mucosa-associated bacteria form a specific environment in the gut; their disruption may play a crucial role in the development of intestinal bowel disease (IBD). Metronidazole, an antibiotic used in the treatment of IBD, alters gut microbiota and reduces basal oxidative stress to proteins in colonic tis...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00916.x

    authors: Pélissier MA,Vasquez N,Balamurugan R,Pereira E,Dossou-Yovo F,Suau A,Pochart P,Magne F

    更新日期:2010-09-01 00:00:00

  • Phylogenetic and clonality analysis of Bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones.

    abstract::Bacillus pumilus is a Gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (PGPR), animal, and human probiotic. However, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. Th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12426

    authors: Branquinho R,Meirinhos-Soares L,Carriço JA,Pintado M,Peixe LV

    更新日期:2014-12-01 00:00:00

  • Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE.

    abstract::We re-evaluated PCR primers targeting nirS, nirK and nosZ genes for denaturing gradient gel electrophoresis as a tool to survey denitrifying community composition in environmental samples. New primers for both nirS and nosZ were combined with existing primers, while for nirK the previously published F1aCu:R3Cu set was...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.04.011

    authors: Throbäck IN,Enwall K,Jarvis A,Hallin S

    更新日期:2004-09-01 00:00:00

  • Identification of the bacterial community involved in methane-dependent denitrification in activated sludge using DNA stable-isotope probing.

    abstract::Methane is used as an alternative carbon source in the denitrification of wastewater lacking organic carbon sources because it is nontoxic and may be efficiently produced by anaerobic biological processes. Methane-dependent denitrification (MDD) in the presence of oxygen requires the co-occurrence of methanotrophy and...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00473.x

    authors: Osaka T,Ebie Y,Tsuneda S,Inamori Y

    更新日期:2008-06-01 00:00:00

  • Effect of temperature change on the composition of the bacterial and archaeal community potentially involved in the turnover of acetate and propionate in methanogenic rice field soil.

    abstract::The microbial community structure was investigated together with the path of methane production in Italian rice field soil incubated at moderate (35 degrees C) and high (45 degrees C) temperature using terminal restriction fragment length polymorphism and stable isotope fractionation. The structure of both the archaea...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00883.x

    authors: Noll M,Klose M,Conrad R

    更新日期:2010-08-01 00:00:00

  • The effect of soil acidification on atmospheric methane uptake by a Maine forest soil(1).

    abstract::Soil from the zone of maximal methanotrophic activity (approximately 5-8 cm depth) in a mixed coniferous-hardwood forest consumed atmospheric methane over a wide pH range (3.5-7.5) with a broad optimum between 4.8 and 6.0. Methane uptake at native soil pH values (4.4-4.8) was only slightly less rapid than rates at opt...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2001.tb00771.x

    authors: Benstead J,King GM

    更新日期:2001-01-01 00:00:00

  • High abundance of Crenarchaeota in a temperate acidic forest soil.

    abstract::The objective of the study was to elucidate the depth distribution and community composition of Archaea in a temperate acidic forest soil. Numbers of Archaea and Bacteria were measured in the upper 18 cm of the soil, and soil cores were sampled on two separate occasions using quantitative PCR targeting 16S rRNA genes....

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2007.00310.x

    authors: Kemnitz D,Kolb S,Conrad R

    更新日期:2007-06-01 00:00:00

  • Metaproteomic and metagenomic analyses of defined oceanic microbial populations using microwave cell fixation and flow cytometric sorting.

    abstract::A major obstacle in the molecular investigation of natural, especially oceanic, microbial cells is their adequate preservation for further land-based molecular analyses. Here, we examined the use of microwaves for cell fixation before high-speed flow cytometric sorting to define the metaproteomes and metagenomes of ke...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00927.x

    authors: Mary I,Oliver A,Skipp P,Holland R,Topping J,Tarran G,Scanlan DJ,O'Connor CD,Whiteley AS,Burkill PH,Zubkov MV

    更新日期:2010-10-01 00:00:00

  • Fine-scale spatial patterns in microbial community composition in an acid mine drainage.

    abstract::Microbial community composition is essential for aquatic ecosystem functions and has been explored across diverse environments and various spatial scales. However, documented patterns are often based on samples from spatially/geographically separated locations or sites. Here, we define sampling volume as spatial scale...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fix124

    authors: Liang JL,Li XJ,Shu HY,Wang P,Kuang JL,Liu J,Zhang MM,Shu WS,Huang LN

    更新日期:2017-10-01 00:00:00

  • Microbial survey of the mummies from the Capuchin Catacombs of Palermo, Italy: biodeterioration risk and contamination of the indoor air.

    abstract::The Capuchin Catacombs of Palermo contain over 1800 preserved bodies dating from the 16th to 20th centuries AD and showing evidence of biodeterioration. An extensive microbiological and molecular investigation was recently performed. Samples were taken from skin, muscle, hair, bone, stuffing materials, clothes, and su...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12165

    authors: Piñar G,Piombino-Mascali D,Maixner F,Zink A,Sterflinger K

    更新日期:2013-11-01 00:00:00

  • Characterization of the bacterial community composition in a hypoxic zone induced by Microcystis blooms in Lake Taihu, China.

    abstract::Cyanobacterial blooms have become more frequent as a result of eutrophication in lakes. The accumulation and breakdown of huge cyanobacterial biomasses often cause hypoxia in lakes. However, little is known about microbial diversity in these areas. In this study, we characterized the bacterial community composition of...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01262.x

    authors: Li H,Xing P,Wu QL

    更新日期:2012-03-01 00:00:00

  • Seasonal dynamics and toxicity of Cylindrospermopsis raciborskii in Lake Guiers (Senegal, West Africa).

    abstract::Cylindrospermopsis raciborskii is a toxic bloom-forming cyanobacterium that occurs at tropical and temperate latitudes. Despite several reports from Africa, no data were previously available about its dynamics or toxic potential there. We therefore carried out a 1-year survey of the dynamics of C. raciborskii in the m...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00141.x

    authors: Berger C,Ba N,Gugger M,Bouvy M,Rusconi F,Couté A,Troussellier M,Bernard C

    更新日期:2006-09-01 00:00:00

  • Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis.

    abstract::Using different techniques of molecular biology we investigated the bacterial diversity of the chemocline of the meromictic Lake Cadagno. Cloning of a total community 16S rDNA PCR product and subsequent screening with a combination of amplified ribosomal DNA restriction analysis and temporal temperature gradient gel e...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00682.x

    authors: Bosshard PP,Santini Y,Grüter D,Stettler R,Bachofen R

    更新日期:2000-02-01 00:00:00

  • Impact of chromium-contaminated wastewaters on the microbial community of a river.

    abstract::The influence of chromium on the microbial community structure was analyzed in a river system subjected to long-term chromium contamination, by plating and by sequencing 16S rRNA genes cloned from DNA extracted from the river sediments. We also analyzed the influence of chromium on the ability of the microbial communi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2005.02.014

    authors: Branco R,Chung AP,Veríssimo A,Morais PV

    更新日期:2005-09-01 00:00:00

  • Heterogeneity of carbon loss and its temperature sensitivity in East-European subarctic tundra soils.

    abstract::Arctic peatlands store large stocks of organic carbon which are vulnerable to the climate change but their fate is uncertain. There is increasing evidence that a part of it will be lost as a result of faster microbial mineralization. We studied the vulnerability of 3500-5900 years old bare peat uplifted from permafros...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw140

    authors: Diáková K,Čapek P,Kohoutová I,Mpamah PA,Bárta J,Biasi C,Martikainen PJ,Šantrůčková H

    更新日期:2016-09-01 00:00:00

  • Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community.

    abstract::Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00806.x

    authors: Durand L,Zbinden M,Cueff-Gauchard V,Duperron S,Roussel EG,Shillito B,Cambon-Bonavita MA

    更新日期:2010-02-01 00:00:00