Improved isolation strategies allowed the phenotypic differentiation of two Nitrospira strains from widespread phylogenetic lineages.


:The second step of nitrification, the oxidation of nitrite to nitrate, is vital for the functioning of the nitrogen cycle, but our understanding of the ecological roles of the involved microorganisms is still limited. The known diversity of Nitrospira, the most widely distributed nitrite-oxidizing bacteria, has increased remarkably by analyses of 16S rRNA and functional gene sequences. However, only few representatives could be brought into laboratory cultures so far. In this study, two Nitrospira from activated sludge were isolated using novel approaches together with established methods. Highly enriched 'Candidatus Nitrospira defluvii' was separated from concomitant heterotrophs by taking advantage of its resistance against ampicillin and acriflavine. Beside this member of lineage I, a novel species of lineage II, named N. lenta, was initially enriched at 10°C and finally purified by using optical tweezers. The tolerance to elevated nitrite levels was much higher in N. defluvii than in the more fastidious N. lenta and was accompanied by pronounced biofilm formation. Phylogenetic classification of 12 additional enrichments indicated that Nitrospira lineage I is common in extreme and moderate ecosystems like lineage II. The new cultures will help to explore physiological and genomic differences affecting niche separation between members of this highly diverse genus.


FEMS Microbiol Ecol


Nowka B,Off S,Daims H,Spieck E




Has Abstract


2015-03-01 00:00:00












  • High abundance of Crenarchaeota in a temperate acidic forest soil.

    abstract::The objective of the study was to elucidate the depth distribution and community composition of Archaea in a temperate acidic forest soil. Numbers of Archaea and Bacteria were measured in the upper 18 cm of the soil, and soil cores were sampled on two separate occasions using quantitative PCR targeting 16S rRNA genes....

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Kemnitz D,Kolb S,Conrad R

    更新日期:2007-06-01 00:00:00

  • Diversity estimates of microeukaryotes below the chemocline of the anoxic Mariager Fjord, Denmark.

    abstract::Microbial communities of extreme environments have often been assumed to have low species richness. We analysed 18S rRNA gene signatures in a sample collected below the chemocline of the anoxic Mariager Fjord in Denmark, and from these data we computed novel parametric and standard nonparametric estimates of protistan...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Zuendorf A,Bunge J,Behnke A,Barger KJ,Stoeck T

    更新日期:2006-12-01 00:00:00

  • Phylogenetic and clonality analysis of Bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones.

    abstract::Bacillus pumilus is a Gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (PGPR), animal, and human probiotic. However, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. Th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Branquinho R,Meirinhos-Soares L,Carriço JA,Pintado M,Peixe LV

    更新日期:2014-12-01 00:00:00

  • Bacterial invasion potential in water is determined by nutrient availability and the indigenous community.

    abstract::In drinking water (DW) and the distribution systems, bacterial growth and biofilm formation have to be controlled both for limiting taste or odour development and preventing clogging or biocorrosion problems. After a contamination with undesired bacteria, factors like nutrient availability and temperature will influen...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Van Nevel S,De Roy K,Boon N

    更新日期:2013-09-01 00:00:00

  • Genomic characterisation of environmental Pseudomonas aeruginosa isolated from dental unit waterlines revealed the insertion sequence ISPa11 as a chaotropic element.

    abstract::The bacterium Pseudomonas aeruginosa is well known to have a remarkable adaptive capacity allowing it to colonise many environments. A recent study on environmental isolates from dental unit waterlines (DUWLs) hinted at a genetic clustering into two groups. Isolates from one of these groups, named cluster III, were sh...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Vincent AT,Freschi L,Jeukens J,Kukavica-Ibrulj I,Emond-Rheault JG,Leduc A,Boyle B,Jean-Pierre F,Groleau MC,Déziel E,Barbeau J,Charette SJ,Levesque RC

    更新日期:2017-09-01 00:00:00

  • Bulk soil and maize rhizosphere resistance genes, mobile genetic elements and microbial communities are differently impacted by organic and inorganic fertilization.

    abstract::Organic soil fertilizers, such as livestock manure and biogas digestate, frequently contain bacteria carrying resistance genes (RGs) to antimicrobial substances and mobile genetic elements (MGEs). The effects of different fertilizers (inorganic, manure, digestate) on RG and MGE abundance and microbial community compos...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Wolters B,Jacquiod S,Sørensen SJ,Widyasari-Mehta A,Bech TB,Kreuzig R,Smalla K

    更新日期:2018-04-01 00:00:00

  • Spatial and temporal diversity and abundance of ammonia oxidizers in semi-arid and arid soils: indications for a differential seasonal effect on archaeal and bacterial ammonia oxidizers.

    abstract::Besides water, nitrogen is the limiting factor for biomass production in arid ecosystems. Global climatic changes are exacerbating aridity levels, and the response of nitrogen-transforming microorganisms to these changes is not clear yet. Using semi-arid and arid ecosystems as surrogates for conditions of increased ar...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Sher Y,Zaady E,Nejidat A

    更新日期:2013-12-01 00:00:00

  • Salinity as a structuring factor for the composition and performance of bacterioplankton degrading riverine DOC.

    abstract::Abstract The impact of salinity on the composition and functional performance (biomass production, growth efficiency and growth rates) of bacterial communities was investigated using batch cultures growing on dissolved organic carbon from a river draining into the Northern Baltic Sea. The cultures were adjusted to riv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Langenheder S,Kisand V,Wikner J,Tranvik LJ

    更新日期:2003-07-01 00:00:00

  • Elsinochrome A production by the bindweed biocontrol fungus Stagonospora convolvuli LA39 does not pose a risk to the environment or the consumer of treated crops.

    abstract::Biological control as an alternative to chemical pesticides is of increasing public interest. However, to ensure safe use of biocontrol methods, strategies to assess the possible risks need to be developed. The production of toxic metabolites is an aspect which has so far largely been neglected in the risk assessment ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Boss D,Maurhofer M,Schläpfer E,Défago G

    更新日期:2007-01-01 00:00:00

  • Poly-3-hydroxybutyrate (PHB) supports survival and reproduction in starving rhizobia.

    abstract::The carbon that rhizobia in root nodules receive from their host powers both N(2) fixation, which mainly benefits the host, and rhizobium reproduction. Rhizobia also store energy in the lipid poly-3-hydroxybutyrate (PHB), which may enhance rhizobium survival when they are carbon limited, either in nodules or in the so...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Ratcliff WC,Kadam SV,Denison RF

    更新日期:2008-09-01 00:00:00

  • Soil fungal community composition and functional similarity shift across distinct climatic conditions.

    abstract::A large part of ecosystem function in woodland systems depends on soil fungal communities. However, global climate change has the potential to fundamentally alter these communities as fungal species are filtered with changing environmental conditions. In this study, we examined the potential effects of climate on host...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Bui A,Orr D,Lepori-Bui M,Konicek K,Young HS,Moeller HV

    更新日期:2020-12-02 00:00:00

  • Distinct composition signatures of archaeal and bacterial phylotypes in the Wanda Glacier forefield, Antarctic Peninsula.

    abstract::Several studies have shown that microbial communities in Antarctic environments are highly diverse. However, considering that the Antarctic Peninsula is among the regions with the fastest warming rates, and that regional climate change has been linked to an increase in the mean rate of glacier retreat, the microbial d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pessi IS,Osorio-Forero C,Gálvez EJ,Simões FL,Simões JC,Junca H,Macedo AJ

    更新日期:2015-01-01 00:00:00

  • Abundance and activity of nitrate reducers in an arable soil are more affected by temporal variation and soil depth than by elevated atmospheric [CO2].

    abstract::Elevated atmospheric carbon dioxide concentrations ([CO(2) ]) might change the abundance and the function of soil microorganisms in the depth profile of agricultural soils by plant-mediated reactions. The seasonal pattern of abundance and activity of nitrate-reducing bacteria was studied in a Mini-FACE experiment plan...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Marhan S,Philippot L,Bru D,Rudolph S,Franzaring J,Högy P,Fangmeier A,Kandeler E

    更新日期:2011-05-01 00:00:00

  • The effect of grazing and nutrient supply on periphyton associated bacteria.

    abstract::The effects of nutrient additions and grazing by macro-invertebrates on periphyton-associated algae and bacteria were studied by performing an enclosure experiment on three occasions from early spring to summer at mesotrophic Lake Erken and Väddö, at the Swedish Baltic coast. There were significant interactions betwee...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Haglund AL,Hillebrand H

    更新日期:2005-03-01 00:00:00

  • Sheep-urine-induced changes in soil microbial community structure.

    abstract::Soil microbial communities play an important role in nutrient cycling and nutrient availability, especially in unimproved soils. In grazed pastures, sheep urine causes local changes in nutrient concentration which may be a source of heterogeneity in microbial community structure. In the present study, we investigated ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Nunan N,Singh B,Reid E,Ord B,Papert A,Squires J,Prosser JI,Wheatley RE,McNicol J,Millard P

    更新日期:2006-05-01 00:00:00

  • Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater.

    abstract::Autotrophic ammonia-oxidising bacteria (AOB) are a crucial component of the microbial communities of nitrifying wastewater treatment systems. Nitrification is known to occur in reactors of different configuration, but whether AOB communities are different in reactors of different design is unknown. We compared the div...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Rowan AK,Snape JR,Fearnside D,Barer MR,Curtis TP,Head IM

    更新日期:2003-03-01 00:00:00

  • Effect of heat shock treatment on stress tolerance and biocontrol efficacy of Metschnikowia fructicola.

    abstract::The effect of high temperature and oxidative stress on the cell viability of the yeast antagonist, Metschnikowia fructicola was determined. A mild heat shock (HS) pretreatment (30 min at 40 °C) improved the tolerance of M. fructicola to subsequent high temperature (45 °C, 20-30 min) and oxidative stress (0.4 mol L⁻¹ h...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Liu J,Wisniewski M,Droby S,Tian S,Hershkovitz V,Tworkoski T

    更新日期:2011-04-01 00:00:00

  • Geomicrobiology of extremely acidic subsurface environments.

    abstract::Extreme acidophiles (microorganisms with pH optima of < 3) can colonize and exploit subterranean environments, such as abandoned metal sulfide mines, that have the potential for developing widespread or isolated pockets of acidity. Although acidophiles can utilize a wide range of electron donors, inorganic materials (...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Johnson DB

    更新日期:2012-07-01 00:00:00

  • Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp.

    abstract::In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeas...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Brilhante RS,Paiva MA,Sampaio CM,Teixeira CE,Castelo-Branco DS,Leite JJ,Moreira CA,Silva LP,Cordeiro RA,Monteiro AJ,Sidrim JJ,Rocha MF

    更新日期:2011-05-01 00:00:00

  • Conjugal properties of the Sinorhizobium meliloti plasmid mobilome.

    abstract::The biology and biochemistry of plasmid transfer in soil bacteria is currently under active investigation because of its central role in prokaryote adaptation and evolution. In this work, we examined the conjugal properties of the cryptic plasmids present in a collection of the N(2)-fixing legume-symbiont Sinorhizobiu...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Pistorio M,Giusti MA,Del Papa MF,Draghi WO,Lozano MJ,Tejerizo GT,Lagares A

    更新日期:2008-09-01 00:00:00

  • Limited geographic distribution of certain strains of the bioluminescent symbiont Photobacterium leiognathi.

    abstract::Photobacterium leiognathi is a facultative bioluminescent symbiont of marine animals. Strains of P. leiognathi that are merodiploid for the luminescence genes (lux-rib operon) have been previously obtained only from Japan. In contrast, strains bearing a single lux-rib operon have been obtained from all the areas sampl...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Urbanczyk H,Kiwaki N,Furukawa T,Iwatsuki Y

    更新日期:2012-08-01 00:00:00

  • The CTX-M-14 plasmid pHK01 encodes novel small RNAs and influences host growth and motility.

    abstract::The dissemination of extended-spectrum β-lactamases (ESBLs) genes among bacteria is commonly achieved by plasmid conjugation. In the last decade, the CTX-M type enzyme was the most widespread and prevalent ESBLs in the world. In Hong Kong and mainland China, among the commonly found CTX-M-carrying plasmids were pHK01 ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Jiang X,Liu X,Law COK,Wang Y,Lo WU,Weng X,Chan TF,Ho PL,Lau TCK

    更新日期:2017-07-01 00:00:00

  • Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments, Southwest China: evidence from hydrochemical and microbiological study.

    abstract::Aerobic anoxygenic phototrophic bacteria may play a particular role in carbon cycling of aquatic systems. However, little is known about the interaction between aerobic anoxygenic phototrophic bacteria and hydrochemistry in groundwater-surface water exchange systems of subtropical karst catchments. We carried out a de...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Li Q,Song A,Peng W,Jin Z,Müller WEG,Wang X

    更新日期:2017-06-01 00:00:00

  • Abiotic autumnal organic matter deposition and grazing disturbance effects on epilithic biofilm succession.

    abstract::Stream epilithic biofilm community assembly is influenced in part by environmental factors. Autumn leaf deposition is an annual resource subsidy to streams, but the physical effects of leaves settling on epilithic biofilms has not been investigated.We hypothesized that bacterial and microeukaryotic community assembly ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Lang JM,McEwan RW,Benbow ME

    更新日期:2015-06-01 00:00:00

  • Mechanisms and recent advances in biological control mediated through the potato rhizosphere.

    abstract::Potato cultivation has a strategic role as a food source for the human population. Its promising future development relies on improving the control of the numerous microbial diseases that affect its growth. Numerous and recent studies on the potato rhizosphere, mycorrhizosphere and endorhiza reveal the presence of a d...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审


    authors: Diallo S,Crépin A,Barbey C,Orange N,Burini JF,Latour X

    更新日期:2011-03-01 00:00:00

  • Motility and chemotaxis of Pseudomonas sp. B4 towards polychlorobiphenyls and chlorobenzoates.

    abstract::The polychlorinated biphenyl (PCB)-degrading Pseudomonas sp. B4 was tested for its motility and ability to sense and respond to biphenyl, its chloroderivatives and chlorobenzoates in chemotaxis assays. Pseudomonas sp. B4 was attracted to biphenyl, PCBs and benzoate in swarm plate and capillary assays. Chemotaxis towar...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Gordillo F,Chávez FP,Jerez CA

    更新日期:2007-05-01 00:00:00

  • Investigations into peach seedling stunting caused by a replant soil.

    abstract::Replant diseases often occur when pome and stone fruits are grown in soil that had previously been planted with the same or similar plant species. They typically lead to reductions in plant growth, crop yield and production duration. In this project, greenhouse assays were used to identify a peach orchard soil that ca...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Bent E,Loffredo A,Yang JI,McKenry MV,Becker JO,Borneman J

    更新日期:2009-05-01 00:00:00

  • Temporal shifts of the Norway lobster (Nephrops norvegicus) gut bacterial communities.

    abstract::The aim of this study was to investigate the gut bacterial communities of Nephrops norvegicus individuals, using a suite of molecular tools consisting of automated ribosomal intergenic spacer analysis, 16S rRNA gene-internal transcribed spacer clone libraries and FISH. The animals were collected from Pagasitikos Gulf,...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Meziti A,Ramette A,Mente E,Kormas KA

    更新日期:2010-11-01 00:00:00

  • Food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization.

    abstract::A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila and Pse...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Jezbera J,Hornák K,Simek K

    更新日期:2005-05-01 00:00:00

  • Soil microbial composition varies in response to coffee agroecosystem management.

    abstract::Soil microbes are essential to the continued productivity of sustainably managed agroecosystems. In shade coffee plantations, the relationship between soil microbial composition, soil nutrient availability and coffee productivity have been demonstrated, but the effects of management on the composition of the soil micr...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章


    authors: Jurburg SD,Shek KL,McGuire K

    更新日期:2020-09-01 00:00:00