Sulfate-reducing bacterial community response to carbon source amendments in contaminated aquifer microcosms.

Abstract:

:Abstract Microbial sulfate reduction is an important metabolic activity in many reduced habitats. However, little is known about the sulfate-reducing communities inhabiting petroleum hydrocarbon (PHC)-contaminated freshwater aquifer sediments. The purpose of this study was to identify the groups of sulfate-reducing bacteria (SRB) selectively stimulated when sediment from a PHC-contaminated freshwater aquifer was incubated in sulfate-reducing aquifer microcosms that were amended with specific carbon sources (acetate, butyrate, propionate, lactate, and citrate). After 2 months of incubation, the SRB community was characterized using phospholipid fatty acid (PLFA) analysis combined with multivariate statistics as well as fluorescence in situ hybridization (FISH). Molybdate was used to specifically inhibit SRB in separate microcosms to investigate the contribution of non-SRB to carbon source degradation. Results indicated that sulfate reduction in the original sediment was an important process but was limited by the availability of sulfate. Substantially lower amounts of acetate and butyrate were degraded in molybdate treatments as compared to treatments without molybdate, suggesting that SRB were the major bacterial group responsible for carbon source turnover in microcosms. All of the added carbon sources induced changes in the SRB community structure. Members of the genus Desulfobulbus were present but not active in the original sediment but an increase of the fatty acids 15:1omega6c and 17:1omega6c and FISH results showed an enrichment of these bacteria in microcosms amended with propionate or lactate. The appearance of cy17:0 revealed that bacteria affiliated with the Desulfobacteriaceae were responsible for acetate degradation. Desulfovibrio and Desulfotomaculum spp. were not important populations within the SRB community in microcosms because they did not proliferate on carbon sources usually favored by these organisms. Metabolic, PLFA, and FISH results provided information on the SRB community in a PHC-contaminated freshwater environment, which exhibited stimulation patterns similar to other (e.g. marine) environments.

journal_name

FEMS Microbiol Ecol

authors

Kleikemper J,Pelz O,Schroth MH,Zeyer J

doi

10.1111/j.1574-6941.2002.tb01000.x

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

109-18

issue

1

eissn

0168-6496

issn

1574-6941

pii

FEM109

journal_volume

42

pub_type

杂志文章
  • Stable carbon isotope fractionation by acetotrophic sulfur-reducing bacteria.

    abstract::Acetate is the most important intermediate in anaerobic degradation of organic matter. The carbon isotope effects associated with the oxidation of acetate (epsilon(ac)) were examined for four acetotrophic sulfur reducers, Desulfuromonas acetoxidans, Desulfuromonas thiophila, Desulfurella acetivorans, and Hippea mariti...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2009.00811.x

    authors: Goevert D,Conrad R

    更新日期:2010-02-01 00:00:00

  • Deposition and postdeposition mechanisms as possible drivers of microbial population variability in glacier ice.

    abstract::Glaciers accumulate airborne microorganisms year by year and thus are good archives of microbial communities and their relationship to climatic and environmental changes. Hypotheses have focused on two possible drivers of microbial community composition in glacier systems. One is aeolian deposition, in which the micro...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6941.2009.00759.x

    authors: Xiang SR,Shang TC,Chen Y,Yao TD

    更新日期:2009-11-01 00:00:00

  • The bacterial microbiota of Stolotermes ruficeps (Stolotermitidae), a phylogenetically basal termite endemic to New Zealand.

    abstract::Stolotermes ruficeps is a widespread, primitive, lower termite occupying dead and decaying wood of many tree species in New Zealand's temperate forests. We identified core bacterial taxa involved in gut processes through combined DNA- and RNA (cDNA)-based pyrosequencing analysis of the 16S nucleotide sequence from fiv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12424

    authors: Reid NM,Addison SL,West MA,Lloyd-Jones G

    更新日期:2014-12-01 00:00:00

  • Physiological response to temperature changes of the marine, sulfate-reducing bacterium Desulfobacterium autotrophicum.

    abstract::The physiological response of bacteria to temperature is critical for the regulation of biogeochemical processes on daily, seasonal, and inter-annual time scales. We investigated the temperature response of the marine sulfate-reducing bacterium Desulfobacterium autotrophicum strain HRM2. Growth experiments in a temper...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2002.tb01030.x

    authors: Rabus R,Brüchert V,Amann J,Könneke M

    更新日期:2002-12-01 00:00:00

  • Salinity as a structuring factor for the composition and performance of bacterioplankton degrading riverine DOC.

    abstract::Abstract The impact of salinity on the composition and functional performance (biomass production, growth efficiency and growth rates) of bacterial communities was investigated using batch cultures growing on dissolved organic carbon from a river draining into the Northern Baltic Sea. The cultures were adjusted to riv...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00149-1

    authors: Langenheder S,Kisand V,Wikner J,Tranvik LJ

    更新日期:2003-07-01 00:00:00

  • Heterogeneity of carbon loss and its temperature sensitivity in East-European subarctic tundra soils.

    abstract::Arctic peatlands store large stocks of organic carbon which are vulnerable to the climate change but their fate is uncertain. There is increasing evidence that a part of it will be lost as a result of faster microbial mineralization. We studied the vulnerability of 3500-5900 years old bare peat uplifted from permafros...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw140

    authors: Diáková K,Čapek P,Kohoutová I,Mpamah PA,Bárta J,Biasi C,Martikainen PJ,Šantrůčková H

    更新日期:2016-09-01 00:00:00

  • Lack of detection of ampicillin resistance gene transfer from Bt176 transgenic corn to culturable bacteria under field conditions.

    abstract::Population levels of total and ampicillin-resistant culturable bacteria and the putative horizontal bla gene acquirement from Bt-corn were studied in commercial fields of transgenic corn in Spain during the years 2000-2003. Commercial fields consisting of conventional corn (Dracma) and Bt176 transgenic corn (Compa CB)...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.01.005

    authors: Badosa E,Moreno C,Montesinos E

    更新日期:2004-05-01 00:00:00

  • Bacterial and archaeal communities in long-term contaminated surface and subsurface soil evaluated through coextracted RNA and DNA.

    abstract::Soil RNA and DNA were coextracted along a contamination gradient at a landfarming field with aged crude oil contamination to investigate pollution-dependent differences in 16S rRNA and rRNA gene pools. Microbial biomass correlated with nucleic acid yields as well as bacterial community change, indicating that the same...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12376

    authors: Mikkonen A,Santalahti M,Lappi K,Pulkkinen AM,Montonen L,Suominen L

    更新日期:2014-10-01 00:00:00

  • Multiple physiological states of a Pseudomonas fluorescens DR54 biocontrol inoculant monitored by a new flow cytometry protocol.

    abstract::A new fluorescence staining and flow cytometry protocol was developed to monitor several physiological states in biocontrol strain Pseudomonas fluorescens DR54 during storage survival in a stationary-phase culture, preparation of clay carrier for seed formulation, and establishment in a sugar beet spermosphere. The hi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2008.00631.x

    authors: Nielsen TH,Sjøholm OR,Sørensen J

    更新日期:2009-03-01 00:00:00

  • Food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization.

    abstract::A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila and Pse...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.12.001

    authors: Jezbera J,Hornák K,Simek K

    更新日期:2005-05-01 00:00:00

  • The bottlenose dolphin (Tursiops truncatus) faecal microbiota.

    abstract::Cetaceans have evolved from herbivorous terrestrial artiodactyls closely related to ruminants and hippopotamuses. Delphinidae, a family included in this order, represent an extreme and successful re-adaptation of mammalian physiology to the marine habitat and piscivorous diet. The anatomical aspects of Delphinidae suc...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiw055

    authors: Soverini M,Quercia S,Biancani B,Furlati S,Turroni S,Biagi E,Consolandi C,Peano C,Severgnini M,Rampelli S,Brigidi P,Candela M

    更新日期:2016-04-01 00:00:00

  • Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils.

    abstract::Phylogenetic analyses of ribosomal RNA gene sequences (rDNAs) retrieved from an Australian desert soil sample (Sturt National Park) revealed the presence of a number of clones which branched deeply from the high GC Gram-positive division line of descent. The most abundant group of these clones were related to Rubrobac...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2000.tb00733.x

    authors: Holmes AJ,Bowyer J,Holley MP,O'Donoghue M,Montgomery M,Gillings MR

    更新日期:2000-08-01 00:00:00

  • Survey of selected antibiotic resistance genes in agricultural and non-agricultural soils in south-central Idaho.

    abstract::Improving our understanding of antibiotic resistance in soils is important for the protection of human, animal and ecological health. In south-central Idaho, antibiotic resistance genes (ARGs) [blaCTX-M-1, erm(B), sul1, tet(B), tet(M) and tet(X)] and a class 1 integron-integrase gene (intI1) were quantified in agricul...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiz071

    authors: Dungan RS,Strausbaugh CA,Leytem AB

    更新日期:2019-06-01 00:00:00

  • Specialized microbiome of the cave-dwelling sponge Plakina kanaky (Porifera, Homoscleromorpha).

    abstract::The recent description of the polychromatic sponge Plakina kanaky revealed original microsymbionts, with some morphotypes recorded for the first time in Homoscleromorpha and others never before observed in other sponge groups. Illumina 16S amplicon sequencing was used to characterize this microbial community by compar...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa043

    authors: Ruiz C,Villegas-Plazas M,Thomas OP,Junca H,Pérez T

    更新日期:2020-04-01 00:00:00

  • Effect of temperature change on the composition of the bacterial and archaeal community potentially involved in the turnover of acetate and propionate in methanogenic rice field soil.

    abstract::The microbial community structure was investigated together with the path of methane production in Italian rice field soil incubated at moderate (35 degrees C) and high (45 degrees C) temperature using terminal restriction fragment length polymorphism and stable isotope fractionation. The structure of both the archaea...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2010.00883.x

    authors: Noll M,Klose M,Conrad R

    更新日期:2010-08-01 00:00:00

  • Impacts of heavy metal contamination and phytoremediation on a microbial community during a twelve-month microcosm experiment.

    abstract::The effects of heavy metals and phytoextraction practices on a soil microbial community were studied during 12 months using a hyperaccumulating plant (Thlaspi caerulescens) grown in an artificially contaminated soil. The 16S ribosomal RNA genes of the Bacteria and the beta-Proteobacteria and the amoA gene (encoding th...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2004.02.004

    authors: Gremion F,Chatzinotas A,Kaufmann K,Von Sigler W,Harms H

    更新日期:2004-05-01 00:00:00

  • Occurrence and expression of bacterial human virulence gene homologues in natural soil bacteria.

    abstract::The presence and in vitro expression of homologues to 22 bacterial human virulence determinants amongst culturable soil bacteria were investigated. About 25% of the bacterial isolates contained virulence gene homologues representing toxin (hblA, cytK2), adhesin (fimH), regulator (phoQ) and resistance (yfbI) determinan...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12413

    authors: Søborg DA,Hendriksen NB,Kroer N

    更新日期:2014-11-01 00:00:00

  • Effect of temperature stress on structure and function of the methanogenic archaeal community in a rice field soil.

    abstract::The effect of a brief (24 h) temperature shift to 4 degrees C (low-temperature stress) or 50 degrees C (high-temperature stress) was studied in methanogenic slurries of Italian rice field soil incubated at either constant 30 degrees C or 15 degrees C. Low-temperature (low-T) stress showed no effect in either the 30 de...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2002.tb00923.x

    authors: Wu XL,Chin KJ,Conrad R

    更新日期:2002-03-01 00:00:00

  • Bacterial community assembly in Atlantic cod larvae (Gadus morhua): contributions of ecological processes and metacommunity structure.

    abstract::Many studies demonstrate the importance of the commensal microbiomes to animal health and development. However, the initial community assembly process is poorly understood. It is unclear to what extent the hosts select for their commensal microbiota, whether stochastic processes contribute, and how environmental condi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiaa163

    authors: Vestrum RI,Attramadal KJK,Vadstein O,Gundersen MS,Bakke I

    更新日期:2020-09-01 00:00:00

  • Comparison of the molecular diversity of the methanogenic community at the brackish and marine ends of a UK estuary.

    abstract::Abstract The 16S rRNA sequence diversity of the euryarchaeal community in a predominately freshwater sediment at East Hill Bridge (EHB) on the River Colne estuary, Essex, UK was investigated and compared to that from marine sediments at the mouth of the river (Colne Point). The East Hill Bridge sediments appear to sup...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2002.tb00902.x

    authors: Purdy KJ,Munson MA,Nedwell DB,Martin Embley T

    更新日期:2002-01-01 00:00:00

  • Potential rates and pathways of microbial nitrate reduction in coastal sediments.

    abstract::Nitrate reduction plays a key role in the biogeochemical dynamics and microbial ecology of coastal sediments. Potential rates of nitrate reduction were measured on undisturbed sediment slices from two eutrophic coastal environments using flow-through reactors (FTR). Maximum potential nitrate reduction rates ranged ove...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2006.00155.x

    authors: Laverman AM,Van Cappellen P,van Rotterdam-Los D,Pallud C,Abell J

    更新日期:2006-11-01 00:00:00

  • Impact of chromium-contaminated wastewaters on the microbial community of a river.

    abstract::The influence of chromium on the microbial community structure was analyzed in a river system subjected to long-term chromium contamination, by plating and by sequencing 16S rRNA genes cloned from DNA extracted from the river sediments. We also analyzed the influence of chromium on the ability of the microbial communi...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/j.femsec.2005.02.014

    authors: Branco R,Chung AP,Veríssimo A,Morais PV

    更新日期:2005-09-01 00:00:00

  • Primers for overlooked nirK, qnorB, and nosZ genes of thermophilic Gram-positive denitrifiers.

    abstract::Although efforts have been made the past few years, knowledge on genomic and phenotypic diversity and occurrence of the denitrification ability in Gram-positive bacteria are still fragmentary. Many environmental monitoring approaches have used nir, nor, and nos genes as marker genes for detection of denitrification or...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12346

    authors: Verbaendert I,Hoefman S,Boeckx P,Boon N,De Vos P

    更新日期:2014-07-01 00:00:00

  • Inferring community dynamics of organohalide-respiring bacteria in chemostats by covariance of rdhA gene abundance.

    abstract::We have developed a novel approach to identifying and quantifying closely related organohalide-respiring bacteria. Our approach made use of the unique genomic associations of specific reductive dehalogenase subunit A encoding genes (rdhA) that exist in known strains of Dehalococcoides mccartyi and Desulfitobacterium a...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/1574-6941.12235

    authors: Marshall IP,Azizian MF,Semprini L,Spormann AM

    更新日期:2014-02-01 00:00:00

  • Investigations of Rhizobium biofilm formation.

    abstract::The development of nitrogen-fixing nodules of the Rhizobium-legume symbiosis, especially the early stages of root hair deformation and curling, infection thread formation, and nodule initiation, has been well studied from a genetic standpoint. In contrast, the factors important for the colonization of surfaces by rhiz...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2005.00044.x

    authors: Fujishige NA,Kapadia NN,De Hoff PL,Hirsch AM

    更新日期:2006-05-01 00:00:00

  • Stable symbionts across the HMA-LMA dichotomy: low seasonal and interannual variation in sponge-associated bacteria from taxonomically diverse hosts.

    abstract::Marine sponges host bacterial communities with important ecological and economic roles in nature and society, yet these benefits depend largely on the stability of host-symbiont interactions and their susceptibility to changing environmental conditions. Here, we investigated the temporal stability of complex host-micr...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv115

    authors: Erwin PM,Coma R,López-Sendino P,Serrano E,Ribes M

    更新日期:2015-10-01 00:00:00

  • Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater.

    abstract::Autotrophic ammonia-oxidising bacteria (AOB) are a crucial component of the microbial communities of nitrifying wastewater treatment systems. Nitrification is known to occur in reactors of different configuration, but whether AOB communities are different in reactors of different design is unknown. We compared the div...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2003.tb01059.x

    authors: Rowan AK,Snape JR,Fearnside D,Barer MR,Curtis TP,Head IM

    更新日期:2003-03-01 00:00:00

  • The enrichment of an alkaliphilic biofilm consortia capable of the anaerobic degradation of isosaccharinic acid from cellulosic materials incubated within an anthropogenic, hyperalkaline environment.

    abstract::Anthropogenic hyperalkaline sites provide an environment that is analogous to proposed cementitious geological disposal facilities (GDF) for radioactive waste. Under anoxic, alkaline conditions cellulosic wastes will hydrolyze to a range of cellulose degradation products (CDP) dominated by isosaccharinic acids (ISA). ...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1093/femsec/fiv085

    authors: Charles CJ,Rout SP,Garratt EJ,Patel K,Laws AP,Humphreys PN

    更新日期:2015-08-01 00:00:00

  • New insight into stratification of anaerobic methanotrophs in cold seep sediments.

    abstract::Methane seepages typically harbor communities of anaerobic methane oxidizers (ANME); however, knowledge about fine-scale vertical variation of ANME in response to geochemical gradients is limited. We investigated microbial communities in sediments below a white microbial mat in the G11 pockmark at Nyegga by 16S rRNA g...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1111/j.1574-6941.2011.01153.x

    authors: Roalkvam I,Jørgensen SL,Chen Y,Stokke R,Dahle H,Hocking WP,Lanzén A,Haflidason H,Steen IH

    更新日期:2011-11-01 00:00:00

  • Effect of anoxia and high sulphide concentrations on heterotrophic microbial communities in reduced surface sediments (Black Spots) in sandy intertidal flats of the German Wadden Sea.

    abstract::Abstract Black reduced sediment surfaces (Black Spots) in sandy intertidal flats of the German Wadden Sea (southern North Sea) are characterised by elevated sulphide concentrations (up to 20 mM) and low redox potentials. It is assumed that the appearance of Black Spots is linked to elevated levels of organic matter co...

    journal_title:FEMS microbiology ecology

    pub_type: 杂志文章

    doi:10.1016/S0168-6496(03)00076-X

    authors: Freitag TE,Klenke T,Krumbein WE,Gerdes G,Prosser JI

    更新日期:2003-06-01 00:00:00