Abstract：
:In this study, cell-material adhesive strength and cellular mechanical properties were measured using atomic force microscopy (AFM) to track cell attachment and osteogenic differentiation. First, chitosan substrates were treated with simulated body fluid (SBF) for various periods, resulting in substrates with different osteoconductivity. The X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS) and in vitro tests revealed that the biomimeticity and osteoconductivity of substrates increased with increasing time of SBF treatment. When the SBF immersion exceeded 14 days, the chitosan substrates exhibited their highest biocompatibility and osteoconductivity. AFM measurements indicated specifically high adhesive forces between SBF-treated chitosan and osteogenic cells, causing better cell attachment. The results demonstrate that cell adhesion was controlled by cell-material adhesive strength, which were in turn controlled via the SBF treatment time. The adhesive strength between cells and material also accounted for the chitosan substrates' specific selectivity toward osteogenic cells. A two-step increase in mechanical strength was observed for the nucleus and cytoplasm of osteogenic cells. The results indicate that through the use of AFM, the real-time cell-material interforce and cellular mechanics can be identified. The adhesive strength was positively correlated to the cell attachment, and the second increase in the Young's modulus of nucleus and cytoplasm was correlated to early osteogenic differentiation.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Ho MH,Li CH,Hsiao SW,Thien DVdoi
10.1007/s10439-014-1162-xsubject
Has Abstractpub_date
2015-04-01 00:00:00pages
1024-35issue
4eissn
0090-6964issn
1573-9686journal_volume
43pub_type
杂志文章abstract：:Literature review articles provide a valuable mechanism for remaining informed amidst an ever-increasing body of scientific work. Condensing current advances into this disseminatable form is a critical activity for any research trainee. To systematize this multifaceted process, we present the "why, when, who, what, ho...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02290-5
更新日期：2019-11-01 00:00:00
abstract：:Analysis and classification of esophageal motility records were investigated using signal processing and fuzzy-set pattern recognition techniques. A set of parameters has been extracted from the raw records and has previously been used as characterizing features. Improvements to these procedures were obtained by extra...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368227
更新日期：1994-01-01 00:00:00
abstract：:Arterial responses to diverse pathologies and insults likely occur via similar mechanisms. For example, many studies suggest that the natural process of aging and isolated systolic hypertension share many characteristics in arteries, including loss of functional elastin, decreased smooth muscle tone, and altered rates...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0287-4
更新日期：2011-07-01 00:00:00
abstract：:Lung cancer is a leading cause of death worldwide. Radiation therapy (RT) is one method to treat this disease. A common side effect of RT for lung cancer is radiation-induced lung damage (RILD) which leads to loss of lung function. RILD often compounds pre-existing smoking-related regional lung function impairment. It...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02697-5
更新日期：2020-11-30 00:00:00
abstract：:In an effort to share best practices in undergraduate engineering design education, we describe the origin, evolution and the current status of the undergraduate biomedical engineering design team program at Johns Hopkins University. Specifically, we describe the program and judge the quality of the pedagogy by relati...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0781-y
更新日期：2013-09-01 00:00:00
abstract：:A method of investigating head acceleration and intracranial dynamics from stick impacts in girls' and women's lacrosse was developed using headform impact experiments and a finite element head model. Assessing the likelihood of head injury due to stick-head impacts is of interest in girls' and women's lacrosse due to...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-014-1091-8
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9652-y
更新日期：2009-05-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368415
更新日期：1990-01-01 00:00:00
abstract：:Correct development of neuronal tracts requires the coordination of multiple permissive and inhibitory signals. By generating an in vitro microenvironment using soft lithography and microfluidic techniques, multiple guidance cues can be presented in a spatially defined way. Here we evaluated how neurites of dorsal roo...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9486-z
更新日期：2008-06-01 00:00:00
abstract：:We investigate the potential of prosthetic heart valves to generate abnormal flow and stress patterns, which can contribute to platelet activation and lysis according to blood damage accumulation mechanisms. High-resolution velocity measurements of the unsteady flow field, obtained with a standard particle image veloc...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0329-y
更新日期：2011-09-01 00:00:00
abstract：:In this paper, we present the application of a semi-global inverse method for determining material parameters of biological tissues. The approach is based on the successive response surface method, and is illustrated by fitting constitutive parameters to two nonlinear anisotropic constitutive equations, one for aortic...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-8338-3
更新日期：2005-12-01 00:00:00
abstract：:This experimental work focused on the sensor selection for the development of a needle-like instrument to treat small isolated cartilage defects with hydrogels. The aim was to identify the most accurate and sensitive imaging method to determine the location and size of defects compared to a gold standard (µCT). Only i...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-2073-z
更新日期：2018-11-01 00:00:00
abstract：:Lung cancer patients often suffer from severe airway stenosis, the symptoms of which can be relieved by the implantation of stents. Different respiratory stents are commercially available, but the impact of their mechanical performance on tissue responses is not well understood. Two novel laser-cut and hand-braided ni...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02278-1
更新日期：2019-08-01 00:00:00
abstract：:Of the many models of peripheral nerve in the literature, essentially all have relied on experimentally derived values for the anisotropic resistivity of the nerve bundle interstitial space. This paper is a comprehensive mathematical analysis of the most referenced experiment for the longitudinal and radial resistivit...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368054
更新日期：1989-01-01 00:00:00
abstract：:Quantitative analysis of breast morphometry is critical to breast plastic surgery. Recently, three-dimensional (3D) photography has emerged as a strong new alternative for breast morphometry analysis in comparison to other existing techniques. 3D photography enables the capture of the entire breast surface topology vi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9971-z
更新日期：2010-05-01 00:00:00
abstract：:This paper reviews the applications of accelerometers on the detection of physiological acoustic signals such as heart sounds, respiratory sounds, and gastrointestinal sounds. These acoustic signals contain a rich reservoir of vital physiological and pathological information. Accelerometer-based systems enable continu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-014-1111-8
更新日期：2014-11-01 00:00:00
abstract：:The blood-brain barrier (BBB) is a major obstacle for drug delivery to the brain. To seek for in vitro BBB models that are more accessible than animals for investigating drug transport across the BBB, we compared four in vitro cultured cell models: endothelial monoculture (bEnd3 cell line), coculture of bEnd3 and prim...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0023-5
更新日期：2010-08-01 00:00:00
abstract：:We developed muscle-tendon models incorporating Hill-type structure and length-dependent coupling between activation and velocity. The models were evaluated in electrically stimulated cat soleus muscles. Dynamic model parameters were estimated by a nonlinear parameter estimation algorithm from input-output data obtain...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.93
更新日期：1998-05-01 00:00:00
abstract：:Finite element analysis is a powerful tool for investigating the biomechanics of atherosclerosis and has thereby provided an improved understanding of acute myocardial infarction. Structural analysis of arterial walls is traditionally performed using geometry contours derived from histology. In this paper we demonstra...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/b:abme.0000049034.75368.4a
更新日期：2004-11-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9756-4
更新日期：2009-10-01 00:00:00
abstract：:Continuous measurement of arterial blood density after bolus injection of fluids of different density into the right atrium has been used to measure cardiac output and mean transit time through the central circulation. The transit time distribution for density, however, differs from that for plasma-phase tracers such ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368628
更新日期：1993-07-01 00:00:00
abstract：:Fox and Lu derived an algorithm based on stochastic differential equations for approximating the kinetics of ion channel gating that is simpler and faster than "exact" algorithms for simulating Markov process models of channel gating. However, the approximation may not be sufficiently accurate to predict statistics of...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9635-z
更新日期：2009-04-01 00:00:00
abstract：:Conventional nanoporous hydrogels often lead to slow cartilage deposition by MSCs in 3D due to physical constraints and requirement for degradation. Our group has recently reported macroporous gelatin microribbon (μRB) hydrogels, which substantially accelerate MSC-based cartilage formation in vitro compared to convent...
journal_title：Annals of biomedical engineering
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doi：10.1007/s10439-020-02522-z
更新日期：2020-07-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9202-9
更新日期：2007-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9212-7
更新日期：2007-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:Deep tissue injury (DTI) is a type of pressure ulcer in which tissue breakdown initiates at the bone-muscle interface under intact skin. Excessive deformation in the soft tissue, particularly around bony prominences, is believed to be one of the causes leading to the development of DTI. The main goal of this study was...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0539-y
更新日期：2012-08-01 00:00:00
abstract：:Our 3-D model for a junctional complex (JC) in the erythrocyte membrane skeleton proposed that the helical actin protofilament functions as a mechanical axis for three pairs of αβ spectrin (Sp), and each pair wraps around the protofilament in a back-to-back fashion. The distal end of each Sp is further associated with...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0293-6
更新日期：2011-07-01 00:00:00
abstract：:Membrane introduction mass spectrometry has been applied to inert gas measurements in blood and tissue, but gases with low blood solubility are associated with reduced sensitivity. Countercurrent extraction of inert gases from a blood sample into a water carrier phase has the potential to extract most of the gas sampl...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684170
更新日期：1997-09-01 00:00:00
abstract：:Skeletal muscles can be injured by their own contractions. Such contraction-induced injury, often accompanied by delayed onset of muscle soreness, is a leading cause of the loss of mobility in the rapidly increasing population of elderly people. Unlike other types of muscle injuries which hurt almost exclusively those...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-014-1173-7
更新日期：2015-02-01 00:00:00
