Abstract:
:A method for isolating and characterizing intestinal lymphoid cells from colonoscopic biopsies is presented. Intraepithelial lymphocytes were separated from the lamina propria by incubation in edetic acid (EDTA) and lamina propria lymphoid cells isolated by incubation in collagenase followed by Ficoll-Hypaque density flotation. Quantitation of T lymphocyte helper (OKT4) and suppressor (OKT8) cells was performed using monoclonal antibodies to cell surface markers and analyzed on a flow cytometer. The isolation procedure yielded approximately 400,000 lamina propria cells and 100,000 intraepithelial cells per sample, with better than 90% viability. Surface marker analysis demonstrated significant differences in the ratios of helper to suppressor cells between the intraepithelial lymphocytes and the lamina propria lymphocytes. These demonstrate the feasibility of lymphoid cell isolation from colonoscopic biopsy specimens for surface marker analysis by flow cytofluorimetry. These techniques could prove important in the study of immune mechanisms in inflammatory bowel diseases.
journal_name
Dig Dis Scijournal_title
Digestive diseases and sciencesauthors
Comer GM,Ramey WG,Kotler DP,Holt PRdoi
10.1007/BF01300700subject
Has Abstractpub_date
1986-02-01 00:00:00pages
151-6issue
2eissn
0163-2116issn
1573-2568journal_volume
31pub_type
杂志文章abstract::The effects of ecabet sodium (ecabet), 12-sulfodehydroabietic acid monosodium salt, on gastric mucin biosynthesis in rat antrum were compared with those in the corpus. Intragastric administration of ecabet significantly increased [3H]glucosamine incorporation into antral mucin as well as into corpus mucin during five ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1005469913079
更新日期:2000-03-01 00:00:00
abstract::To evaluate the frequency and type of gastroduodenal mucosal inflammatory changes in patients with symptomatic acid peptic disease and nonulcer dyspepsia, 100 patients with gastrointestinal symptoms indicative of acid peptic disease or dyspepsia and 10 control subjects were clinically evaluated and underwent radiograp...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01308325
更新日期:1980-09-01 00:00:00
abstract::The major heat shock protein, HSP70, is known to be involved in cytoprotection against environmental stresses mediated by their function as a "molecular chaperone." However, the influence of HSP70 on gastric mucosal healing under physical stimulation or stress is not completely understood. Rat gastric mucosal cells (R...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-007-9795-3
更新日期:2007-11-01 00:00:00
abstract::Our aim was to determine the effect of intestinal transection and resection on the prevalence of enteric flora and evaluate whether any such changes alter luminal SCFA and lactic acid content. Dogs underwent either 50% proximal (PR, N = 6) or distal (DR, N = 7) resection, distal resection with bypass of the ileocecal ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1018831728734
更新日期:1998-03-01 00:00:00
abstract::A multitude of opportunistic infections has been documented in virtually every organ system of patients with the acquired immunodeficiency syndrome (AIDS). Prominent among these are infections of the gastrointestinal tract. However, studies of large numbers of patients documenting the frequency of such involvement are...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01535791
更新日期:1988-09-01 00:00:00
abstract::Activation of peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to inhibit the proliferation of gastric cancer cells. A common polymorphism at codon 12 of this gene (Pro12Ala) has been shown to confer protection against diabetes and colorectal cancer. We investigated the influence of PPARgamm...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-007-9944-8
更新日期:2008-03-01 00:00:00
abstract::The initiating mechanisms involved in colonic injury are currently unknown. The goal of the current study was to examine the role of the inflammatory mediators reactive oxygen metabolites and proteases in an ex vivo model of selective epithelial permeability. Rats were prepared with exteriorized colonic chambers to wh...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02209017
更新日期:1995-10-01 00:00:00
abstract:BACKGROUND:Triglyceride-rich lipoproteins are important in dietary lipid absorption and subsequent energy distribution in the body. Their importance in the gut-lymph may have been overlooked in sepsis, the most common cause of critical illness, and in gut ischemia-reperfusion injury, a common feature of many critical i...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-018-5270-6
更新日期:2018-12-01 00:00:00
abstract::Small intestinal bacterial overgrowth (SIBO) has a high incidence in dogs and, as in humans, is difficult to diagnose. The aim of this study was to determine the diagnostic significance of serum unconjugated bile acid concentrations in dogs with bacterial overgrowth. Fasting sera were obtained from 23 dogs: 10 with cu...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1005493416946
更新日期:2000-02-01 00:00:00
abstract::This analysis evaluated the association between sleep disturbance and gastrointestinal symptoms in women with and without irritable bowel syndrome (IBS), and examined the role of psychological distress in this relationship. Women with IBS (N = 82) reported considerably higher levels of sleep disturbance compared to co...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1005581226265
更新日期:2000-05-01 00:00:00
abstract::Rectally administered suspensions of 5-aminosalicylic acid (5-ASA) are topically effective in treating left-sided ulcerative colitis. The extent to which the contents of these enemas are distributed to inflamed mucosal linings has not previously been determined. This study was undertaken to validate a technique for la...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01540060
更新日期:1989-11-01 00:00:00
abstract::Many drugs have been used in the treatment of chronic hepatitis B, but with the exception of interferon, none have proved to be effective. Several studies have found that a sustained loss of viral replication occurs in approximately 40% of patients who started with a 16-week course of recombinant interferon alfa-2b gi...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,评审
doi:10.1007/BF01316785
更新日期:1993-04-01 00:00:00
abstract::The prevalence of lymphocytic gastritis, a specific form of chronic gastritis characterized by infiltration of gastric superficial epithelium with T lymphocytes, has been established in nonulcer dyspepsia. Among a population sample of 586 patients at risk for gastric carcinoma, 0.83% of patients with nonulcer dyspepsi...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01297450
更新日期:1991-08-01 00:00:00
abstract::H. pylori may colonize columnar-lined esophagus, although an etiologic role in esophageal adenocarcinoma is unproven. H. pylori can adhere to intestinal metaplasia in the stomach. This study was designed to examine if H. pylori adheres to specialized intestinal metaplasia in columnar-lined esophagus. Esophageal biopsi...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1026628307895
更新日期:1999-06-01 00:00:00
abstract::Liver transplantation is the only effective treatment of familial amyloidotic polyneuropathy type I (FAP). The aim of the present investigation was to identify factors at the time of submission for transplantation that had impact on survival, with special reference to gastrointestinal disturbances. All 28 liver-transp...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF02093589
更新日期:1996-10-01 00:00:00
abstract:INTRODUCTION:Periampullary diverticulum (PAD) is most often asymptomatically found in elderly population. ERCP in the presence of PAD is technically challenging since the location and orientation of the ampulla could be altered. Various studies have reported differing results on the technical success and safety outcome...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章,meta分析
doi:10.1007/s10620-018-5314-y
更新日期:2019-05-01 00:00:00
abstract::Sulfated and unsulfated bile acid composition was studied in serum and bile in 10 patients with alcoholic cirrhosis. Samples, collected before and 2 months after portacaval anastomosis, were analyzed using a gaschromatographic method. Mean total serum bile acid levels rose from 32.0 +/- 5.3 (SE) mumol/liter before to ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01308099
更新日期:1981-06-01 00:00:00
abstract::Using conventional statistical analysis and multiple regression analysis, we investigated the viral and host factors that influence the response to recombinant interferon-alpha 2a therapy in patients with chronic hepatitis C. A total of 36 patients was randomly assigned to three administration schedules, 12 patients i...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02093793
更新日期:1994-06-01 00:00:00
abstract::In view of the increased anticoagulant effect of acetaldehyde-treated heparin, other glycosaminoglycans (GAGs) such as chondroitin sulfates A and C, dermatan sulfate (chondroitin sulfate B), heparan sulfate, and hyaluronic acid were tested for anticoagulant activity before and after exposure to acetaldehyde. Clotting ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1010668005729
更新日期:2001-09-01 00:00:00
abstract::MR enteroclysis is becoming a very important imaging modality in the workup and follow-up of small bowel diseases. The technique has many advantages, including the absence of ionizing radiation, ability to control luminal distension, superior tissue characterization, multiplanar capabilities, and mural and extramural ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-005-2700-z
更新日期:2005-06-01 00:00:00
abstract::Orlistat (tetrahydrolipstatin) is a potent inhibitor of gastric and pancreatic lipase activity causing a diminution of free fatty acids in the intestinal lumen. The release of cholecystokinin (CCK) critically depends on the presence of free fatty acids in the small intestine. Postprandial CCK release and gallbladder c...
journal_title:Digestive diseases and sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF02100135
更新日期:1996-12-01 00:00:00
abstract::Octreotide is an effective therapeutic option in controlling secretory diarrhea of varied etiology. However, marked patient-to-patient differences in the antidiarrheal effects necessitate titration of octreotide dose in individual patients to achieve optimal symptom control. A consensus development panel established g...
journal_title:Digestive diseases and sciences
pub_type: 共识发展会议,杂志文章,评审
doi:10.1007/BF02285194
更新日期:1995-07-01 00:00:00
abstract::Dermatitis herpetiformis (DH) and isolated gluten-sensitive enteropathy (GSE) are gluten-sensitive diseases in which ingestion of dietary gluten results in the development of clinical disease. Patients with DH develop cutaneous IgA deposits and a severe skin disease, but rarely develop gastrointestinal symptoms. Patie...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/a:1005512513007
更新日期:2000-10-01 00:00:00
abstract::Both increased duodenogastric reflux and chronic aspirin ingestion are associated with the development of gastric ulcers in man. Animal studies suggest aspirin increases duodenogastric reflux. Prostaglandin E2 protects gastric mucosa from the effects of many injurious agents and inhibits gastric motility, but its effe...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01320256
更新日期:1985-06-01 00:00:00
abstract::Small bowel tumors are rare, accounting for 1-2% of all gastrointestinal neoplasms. We sought to determine the diagnostic and therapeutic impact of double-balloon enteroscopy (DBE) in patients with small bowel tumors. Between January 2005 and March 2008, 78 patients underwent 96 DBE. All nine patients (seven males; me...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-008-0512-7
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:Liver fibrosis is a common scarring response to chronic liver injury. It is a precursor to cirrhosis and liver carcinoma. Hepatic stimulator substance (HSS), a known liver-specific but species-nonspecific growth factor, has been shown to protect hepatocytes from various toxins. METHODS:We have investigated ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-012-2168-6
更新日期:2012-08-01 00:00:00
abstract::In the pathogenesis of gastroesophageal reflux disease (GERD), gastric acid is considered to be one of the most important factors, but little is known about the degree of gastric acid secretion in GERD patients. In this study, we evaluated it in GERD patients and control subjects by 24-h intragastric pH, and serologic...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1023/b:ddas.0000030090.84412.2a
更新日期:2004-05-01 00:00:00
abstract::Glutamine synthetase is responsible for the ATP-dependent amidation of glutamate to glutamine. In liver the enzyme is highly localized in perivenous hepatocytes; in brain the enzyme is localized in astrocytes. Portacaval anastomosis resulted in liver atrophy, hyperammonemia, and up to 90% loss of glutamine synthetase ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/BF01300297
更新日期:1992-07-01 00:00:00
abstract:INTRODUCTION:Increased nonacid reflux is diagnosed in a subgroup of patients with gastroesophageal reflux disease who often present with reflux symptoms refractory to proton-pump inhibitor therapy. Despite the prevalence of this condition, the management approach for patients with increased nonacid reflux can often be ...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-020-06348-6
更新日期:2020-05-23 00:00:00
abstract:BACKGROUND AND AIM:Dietary fiber shortens gut transit time, but data on the effects of fiber components (including resistant starch, RS) on intestinal contractility are limited. We have examined RS effects in male Sprague-Dawley rats fed either a high-amylose maize starch (HAMS) or a wholemeal made from high-amylose wh...
journal_title:Digestive diseases and sciences
pub_type: 杂志文章
doi:10.1007/s10620-015-3537-8
更新日期:2015-06-01 00:00:00