Abstract：
:Angiotensin-converting enzyme (ACE) activity in the intestinal whole homogenate was showed as three peaks on a column of Sephacryl S-300 HR gel filteration. Over 90% of total ACE activity was found in a soluble fraction separated with an ultracentrifuge of the intestinal homogenate, and the ACE activities were detected as two peaks on the same column. On the other hand, two peaks of ACE activities were found in a membrane-bound fraction of treated with trypsin on the Sephacryl column and confirmed with the two peaks of the soluble fraction, while the fraction extracted with Triton X-100 of the membrane-bound fraction showed only one peak as major peak. All ACE peaks were inhibited by addition of EDTA or captopril and by absence of chloride ion completely. We purified one ACE from the soluble fraction by lisinopril-linked Sepharose 6B affinity column chromatography and Cellulofine GCL-200 gel filteration. This enzyme was a 1323-fold purification and its final recovery was 25%. The molecular weight of this enzyme (180,000) was larger than that of ACE from human kidney (170,000), estimated by 7.5% SDS-PAGE. The Km value of the enzyme for HHL was 2.1 mM. The enzyme activity was competitively inhibited by captopril.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Hayakari M,Amano K,Izumi H,Murakami Sdoi
10.1007/978-1-4615-9546-5_60subject
Has Abstractpub_date
1989-01-01 00:00:00pages
365-70eissn
0065-2598issn
2214-8019journal_volume
247Bpub_type
杂志文章abstract：:The rates of ethanol oxidation in various species are linked to the rates of general metabolism, and more specifically that of O2 utilization by the liver. After chronic ethanol administration, increased oxidation of ethanol is accompanied by increased total hepatic O2 utilization, as studied in the whole animal, the ...
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journal_title：Advances in experimental medicine and biology
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abstract：:Curcumin was found to be cytotoxic in nature to a wide variety of tumor cell lines of different tissue origin. The action of curcumin is dependent on with the cell type, the concentration of curcumin (IC50: 2-40 microg/mL), and the time of the treatment. The major mechanism by which curcumin induces cytotoxicity is th...
journal_title：Advances in experimental medicine and biology
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abstract：:A monodispersed and ideal solution is a central (unique?) requirement of SAXS to allow one to extract structural information from the recorded pattern. On-line Size Exclusion Chromatography (SEC) marked a major breakthrough, separating particles present in solution according to their size. Identical frames under an el...
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journal_title：Advances in experimental medicine and biology
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