Abstract:
:A monodispersed and ideal solution is a central (unique?) requirement of SAXS to allow one to extract structural information from the recorded pattern. On-line Size Exclusion Chromatography (SEC) marked a major breakthrough, separating particles present in solution according to their size. Identical frames under an elution peak can be averaged and further processed free from contamination. However, this is not always straightforward, separation is often incomplete and software have been developed to deconvolve the contributions from the different species (molecules or oligomeric forms) within the sample. In this chapter, we present the general workflow of a SEC-SAXS experiment. We present recent instrumental and data analysis improvements that have improved the quality of recorded data, extended its potential and turn it into a mainstream approach. We describe into some details two specific applications of SEC-SAXS that provide more than just separating associated forms from the particle of interest.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Pérez J,Vachette Pdoi
10.1007/978-981-10-6038-0_11subject
Has Abstractpub_date
2017-01-01 00:00:00pages
183-199eissn
0065-2598issn
2214-8019journal_volume
1009pub_type
杂志文章abstract::TRPV1 is a non-selective cation channel gated by noxious heat, vanilloids and extracellular protons, and act as an important signal integrator in sensory nociceptors. Because of its integrative signaling properties in response to inflammatory stimuli, TRPV1 antagonists are predicted to inhibit the sensation of ongoing...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-9373-7_19
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:
更新日期:1995-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-69194-7_6
更新日期:2017-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/978-3-030-42667-5_13
更新日期:2020-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-25474-6_21
更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-94-007-2555-3_14
更新日期:2012-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
doi:10.1007/5584_2019_476
更新日期:2020-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-0135-0_50
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abstract::The effects of ANTU-induced acute pulmonary capillary injury on lung and serum ACE functional activity and the specific accumulation of radio-labelled anti-ACE in lung were explored. Rats were injected either with ANTU or the solvent and sacrificed at various intervals up to one week after injection. All ANTU-injected...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-5143-6_67
更新日期:1986-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-3-319-42316-6_9
更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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abstract::Pigment cells in developing vertebrates are derived from a transient and pluripotent population of cells called neural crest. The neural crest delaminates from the developing neural tube and overlying ectoderm early in development. The pigment cells are the only derivative to migrate along the dorso-lateral pathway. A...
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pub_type: 杂志文章,评审
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-981-13-8023-5_10
更新日期:2019-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4615-4863-8_61
更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
doi:10.1007/978-1-4684-6039-1_23
更新日期:1998-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2017-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2002-01-01 00:00:00