Abstract：
:We have found that apparent Ki values of some, but not all, carboxyalkyl-dipeptide inhibitors of angiotensin converting enzyme decrease as a function of incubation time. The most potent of the ACE inhibitors tested so far is RAC-X-65 (N-[1(S)-carboxy-3-carboxanilidiopropyl]-L-Ala-L-Pro). When RAC-X-65 is not preincubated with human serum ACE (2.4 X 10(-11) M), the apparent Ki value is 4.4 X 10(-10) M. Preincubation of RAC-X-65 with ACE for 15 min before addition of substrate yields an apparent Ki of 4.1 X 10(-11) M. a 90 min preincubation of the inhibitor with ACE yields an apparent Ki of 1.2 X 10(-11) M, i.e., the reaction of the inhibitor with enzyme is virtually stoichiometric. The enzyme:inhibitor complex is poorly separated by molecular sieve chromatography or by dilution. That such tightly bound complexes are formed in vivo is suggested by the following results: The intravenous ED50 (anesthetized rats) of RAC-X-65 is 9.43 nmol/kg, and the time for half recovery (t1/2) of responsiveness to i.v. angiotensin I, 120 ng/kg, following a cumulative dose of 240 nmol/kg of the inhibitor is 165 min. For comparison, the i.v. ED50 of captopril is 105 nmol/kg, and its t1/2 following a cumulative dose of 240 nmol/kg is 16 min. Implied is the possibility that slow tight binding inhibitors of ACE may be used in a 1 pill per day regimen for the treatment of hypertension.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Ryan JW,Chung AY,Berryer P,Murray MA,Ryan JPdoi
10.1007/978-1-4684-5143-6_57subject
Has Abstractpub_date
1986-01-01 00:00:00pages
419-25eissn
0065-2598issn
2214-8019journal_volume
198 Pt Apub_type
杂志文章abstract：:Thermomechanical Analysis (TMA), using the parallel-plate-rheometer mode of the DuPont 943 TMA, was used to evaluate the softening properties of frozen aqueous carbohydrate model systems. A sample (of fixed dimension) of frozen solution was placed between the parallel plates and cooled to -80 degrees C. The sample was...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4899-0664-9_11
更新日期：1991-01-01 00:00:00
abstract：:NCDs including diabetes, heart disease and stroke are global epidemics of the 21st century. The greatest burden on health will be in developing countries and sub-Saharan Africa is an area of major challenge: We are concerned with planning for the adult victims of the new epidemic and this includes the development of a...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4615-1321-6_4
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abstract：:The 1st International Conference on Oral Mucosal Immunity and the Microbiome (OMIM) took place at the Avra Imperial Hotel, Chania, Crete, Greece, between 26th and 30th September 2018, under the auspices of the Aegean Conferences. This was the first Aegean Conference of its kind in thematic oral research, and a unique ...
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doi：10.1007/978-3-030-28524-1_1
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abstract：:The analysis of complex biological networks has traditionally relied on decomposition into smaller, semi-autonomous units such as individual signaling pathways. With the increased scope of systems biology (models), rational approaches to modularization have become an important topic. With increasing acceptance of de f...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4419-7210-1_1
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abstract：:Store-operated Ca2+ entry (SOCE) is a ubiquitous mechanism for Ca2+ influx in mammalian cells with important physiological implications. Since the discovery of SOCE more than three decades ago, the mechanism that communicates the information about the amount of Ca2+ accumulated in the intracellular Ca2+ stores to the ...
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doi：10.1007/978-3-030-12457-1_17
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abstract：:Biochemical imbalances, provoked by aging or a secondary illness, might directly affect the brain, causing severe problems, such as loss of memory or alteration of behavior patterns. Brain disorders are usually classified as injuries (such as stroke, hematomas, and concussions), tumors, and neurodegenerative (such as ...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-319-90143-5_2
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abstract：:The IGF binding proteins (IGFBPs) comprise at least six distinct species which may modulate the action of IGFs. IGFs are important regulators of fetal growth and differentiation. We have studied the mRNA expression of the six IGFBPs during post-implantation embryogenesis (day 11-18) by in situ hybridization techniques...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4615-2988-0_26
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abstract：:Maturation of the fetal lung is a complex process involving interactions between intrinsic biologic development of several cell types and hormonal triggering of biochemical and functional alterations. Catecholamines, corticosteroids, thyroid hormones and perhaps prolactin, all influence the transition from non-functio...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：1991-01-01 00:00:00
abstract：:Multiple myeloma is a hematological malignancy. It is characterized by the abnormal clonal proliferation of malignant plasma cells in the bone marrow and the secretion of a large number of monoclonal immunoglobulins or light chains, causing bone destruction, elevated blood calcium levels, anemia, and renal dysfunction...
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doi：10.1007/978-981-15-4272-5_45
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-319-75402-4_70
更新日期：2018-01-01 00:00:00
abstract：:The solar ultraviolet-B (UVB)/vitamin D/cancer hypothesis was proposed by the brothers Cedric and Frank Garland in 1980. In 2002, the list was increased to 15 types of cancer using data in the 1999 version of the atlas of cancer mortality rates in the United States. Ecological studies of cancer incidence and/or mortal...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4939-0437-2_4
更新日期：2014-01-01 00:00:00
abstract：:Computer-aided diagnosis provides a medical procedure that assists physicians in interpretation of medical images. This work focuses on computer-aided tongue image analysis specifically, based on Traditional Chinese Medicine (TCM). Tongue diagnosis is an important component of TCM. Computerized tongue diagnosis can ai...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-10-5717-5_5
更新日期：2017-01-01 00:00:00
abstract：:What is the function of the Hox genes? At first glance, it is a curious question. Indeed, the answer seems so obvious that several authors have spoken of 'the Hox function' about some of the Hox genes, namely Hox3/zen and Hox6/ftz that seem to have lost it during the evolution of Arthropods. What these authors meant i...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4419-6673-5_12
更新日期：2010-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-030-32633-3_10
更新日期：2020-01-01 00:00:00
abstract：:Based on the insights gleaned from decades of research, it seems clear that mechanistic target of rapamycin (mTOR) is an essential signaling node that integrates environmental clues for regulation of cell survival, metabolism and proliferation of the cells. However, overwhelmingly increasing scientific evidence has ad...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-20301-6_15
更新日期：2019-01-01 00:00:00
abstract：:Cross sectional segments were removed from the uteri of pregnant and pseudopregnant mice and incubated in vitro with labeled amino acids. Proteins that were synthesized and secreted by these explants were separated by two-dimensional polyacrylamide gel electrophoresis and localized by fluorography. As expected, the ov...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-1297-0_12
更新日期：1987-01-01 00:00:00
abstract：:Circular RNAs (circRNAs) are endogenously expressed non-coding RNAs discovered in the early 1990s as a transcriptional by-product of little importance. It was only recently that they were identified as a key player in regulating the gene expression by targeting and modulating the functions of microRNA, a process known...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-981-13-1426-1_18
更新日期：2018-01-01 00:00:00
abstract：:The aim of the study was to explore the possible interrelationship between reactive oxygen species (ROS) formation and cPLA2 activation and the mediator role that [Ca2+]i may play in these processes in the human keratinocyte cell line, HaCaT. HaCaT cells can be invoked to transiently produce ROS by epidermal growth fa...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-1810-9_7
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abstract：:Heterogeneously distributed hypoxic areas are a characteristic property of locally advanced breast cancers. Hypoxia results from an imbalance between the supply and consumption of oxygen (O2). Major pathogenetic mechanisms for the emergence of hypoxia are (i) structural and functional abnormalities in the tumor microv...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/0-387-26206-7_44
更新日期：2005-01-01 00:00:00
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abstract：:The ability to serially collect blood samples and conduct ultrasound examinations in Asian and African elephants has provided unique opportunities to study the biology of these endangered species. As a result, many unique aspects of elephant reproduction have been identified. For females, there are interesting differe...
journal_title：Advances in experimental medicine and biology
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abstract：:This review discusses new developments in Förster resonance energy transfer (FRET) microscopy and its application to cellular receptors. The method is based on the kinetic theory of FRET, which can be used to predict FRET not only in dimers, but also higher order oligomers of donor and acceptor fluorophores. Models ba...
journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-1280-2_13
更新日期：1987-01-01 00:00:00
abstract：:In normal and cancer cells, successful cell division requires accurate duplication of chromosomal DNA. All cells require a multiprotein DNA duplication system (replisomes) for their existence. However, death of normal cells in our body occurs through the apoptotic process. During apoptotic process several crucial gene...
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doi：10.1007/978-981-13-3065-0_15
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abstract：:An ovulated egg of vertebrates is surrounded by unique extracellular matrix, the egg coat or zona pellucida, playing important roles in fertilization and early development. The vertebrate egg coat is composed of two to six zona pellucida (ZP) glycoproteins that are characterized by the evolutionarily conserved ZP-doma...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-981-10-3975-1_5
更新日期：2017-01-01 00:00:00
abstract：:Nerve conduction in myelinated axons is a fascinating subject due to the intricate structure and complex properties of the axon and its relation to the equally complex Schwann cells surrounding it. This chapter first deals with normal functional aspects of voltage-gated ion channels in the axon and Schwann cell membra...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-981-32-9636-7_7
更新日期：2019-01-01 00:00:00
abstract：:V(D)J recombination not only comprises the molecular mechanism that insures diversity of the immune system but also constitutes a critical checkpoint in the developmental program of B- and T-lymphocytes. The analysis of human patients with Severe Combined Immune Deficiency (SCID) has contributed to the understanding o...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-0296-2_4
更新日期：2009-01-01 00:00:00
abstract：:Inhalation of high concentrations of zinc oxide (ZnO) particles may cause metal fume fever. A useful tool to characterize the reactivity of innate immune cells of an individual, e.g., after in vivo exposure, is the whole blood assay (WBA). The measurable outcome of WBA is the release of cytokines, especially pro-infla...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/5584_2019_471
更新日期：2020-01-01 00:00:00
abstract：:Despite diverse and changing extracellular environments, fungi maintain a relatively constant cytosolic pH and numerous organelles of distinct lumenal pH. Key players in fungal pH control are V-ATPases and the P-type proton pump Pma1. These two proton pumps act in concert with a large array of other transporters and a...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-25304-6_3
更新日期：2016-01-01 00:00:00
