Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria.

Abstract:

:Microorganisms possess enormous highly specific metabolic activities, which enable them to utilize and transform nearly every known chemical class present in crude oil. In this context, one of the most studied biocatalytic processes is the biodesulfurization (BDS) of thiophenic sulfur-containing compounds such as benzothiophene (BT) and dibenzothiophene (DBT) in crude oils and refinery streams. Three newly isolated bacterial strains, which were affiliated as Rhodococcus sp. strain SA11, Stenotrophomonas sp. strain SA21, and Rhodococcus sp. strain SA31, were enriched from oil contaminated soil in the presence of DBT as the sole S source. GC-FID analysis of DBT-grown cultures showed consumption of DBT, transient formation of DBT sulfone (DBTO2) and accumulation of 2-hydroxybiphenyl (2-HBP). Molecular detection of the plasmid-borne dsz operon, which codes for the DBT desulfurization activity, revealed the presence of dszA, dszB, and dszC genes. These results point to the operation of the known 4S pathway in the BDS of DBT. The maximum consumption rate of DBT was 11 μmol/g dry cell weight (DCW)/h and the maximum formation rate of 2-HBP formation was 4 μmol/g DCW/h. Inhibition of both cell growth and DBT consumption by 2-HBP was observed for all isolates but SA11 isolate was the least affected. The isolated biocatalysts desulfurized other model DBT alkylated homologs. SA11 isolate was capable of desulfurizing BT as well. Resting cells of SA11 exhibited 10% reduction in total sulfur present in heavy crude oil and 18% reduction in total sulfur present in the hexane-soluble fraction of the heavy crude oil. The capabilities of the isolated bacteria to survive and desulfurize a wide range of S compounds present in crude oil are desirable traits for the development of a robust BDS biocatalyst to upgrade crude oils and refinery streams.

journal_name

Front Microbiol

authors

Mohamed Mel-S,Al-Yacoub ZH,Vedakumar JV

doi

10.3389/fmicb.2015.00112

subject

Has Abstract

pub_date

2015-02-13 00:00:00

pages

112

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • A Plasmid-Transposon Hybrid Mutagenesis System Effective in a Broad Range of Enterobacteria.

    abstract::Random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. Here we develop a system facilitating random transposon mutagenesis in a range of different Gram-negative bacterial strains, including Pectobacterium atrosepticum, Citrobacter rod...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01442

    authors: Monson R,Smith DS,Matilla MA,Roberts K,Richardson E,Drew A,Williamson N,Ramsay J,Welch M,Salmond GP

    更新日期:2015-12-22 00:00:00

  • Biosynthesis of archaeal membrane ether lipids.

    abstract::A vital function of the cell membrane in all living organism is to maintain the membrane permeability barrier and fluidity. The composition of the phospholipid bilayer is distinct in archaea when compared to bacteria and eukarya. In archaea, isoprenoid hydrocarbon side chains are linked via an ether bond to the sn-gly...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2014.00641

    authors: Jain S,Caforio A,Driessen AJ

    更新日期:2014-11-26 00:00:00

  • Vitamin b(1) and b(12) uptake and cycling by plankton communities in coastal ecosystems.

    abstract::While vitamin B(12) has recently been shown to co-limit the growth of coastal phytoplankton assemblages, the cycling of B-vitamins in coastal ecosystems is poorly understood as planktonic uptake rates of vitamins B(1) and B(12) have never been quantified in tandem in any aquatic ecosystem. The goal of this study was t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00363

    authors: Koch F,Hattenrath-Lehmann TK,Goleski JA,Sañudo-Wilhelmy S,Fisher NS,Gobler CJ

    更新日期:2012-10-12 00:00:00

  • IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner.

    abstract::The interferon (IFN) system is one of the most important defensive responses of mammals against viruses, and is rapidly evoked when the pathogen-associated molecular patterns (PAMPs) of viruses are sensed. Non-self, virus-derived RNA species have been identified as the PAMPs of RNA viruses. In the present study, we co...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00804

    authors: Yoshida A,Kawabata R,Honda T,Tomonaga K,Sakaguchi T,Irie T

    更新日期:2015-08-04 00:00:00

  • Enrichment and Characterisation of a Mixed-Source Ethanologenic Community Degrading the Organic Fraction of Municipal Solid Waste Under Minimal Environmental Control.

    abstract::The utilisation of the organic fraction of municipal solid waste as feedstock for bioethanol production could reduce the need for disposal of the ever-increasing amounts of municipal solid waste, especially in developing countries, and fits with the integrated goals of climate change mitigation and transport energy se...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00722

    authors: Carrillo-Barragan P,Bowler B,Dolfing J,Sallis P,Gray ND

    更新日期:2019-04-09 00:00:00

  • Potential Risk Areas of Aedes albopictus in South-Eastern Iran: A Vector of Dengue Fever, Zika, and Chikungunya.

    abstract::The possibility of the rapid and global spread of Zika, chikungunya, yellow fever, and dengue fever by Aedes albopictus is well documented and may be facilitated by changes in climate. To avert and manage health risks, climatic and topographic information can be used to model and forecast which areas may be most prone...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01660

    authors: Nejati J,Bueno-Marí R,Collantes F,Hanafi-Bojd AA,Vatandoost H,Charrahy Z,Tabatabaei SM,Yaghoobi-Ershadi MR,Hasanzehi A,Shirzadi MR,Moosa-Kazemi SH,Sedaghat MM

    更新日期:2017-09-05 00:00:00

  • Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus thermophilus.

    abstract::In streptococci, entry in competence is dictated by ComX abundance. In Streptococcus thermophilus, production of ComX is transient and tightly regulated during growth: it is positively regulated by the cell-cell communication system ComRS during the activation phase and negatively regulated during the shut-off phase b...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01413

    authors: Haustenne L,Bastin G,Hols P,Fontaine L

    更新日期:2015-12-22 00:00:00

  • Orf Virus 002 Protein Targets Ovine Protein S100A4 and Inhibits NF-κB Signaling.

    abstract::Orf virus (ORFV), a member of Parapoxvirus, has evolved various strategies to modulate the immune responses of host cells. The ORFV-encoded protein ORFV002, a regulator factor, has been found to inhibit the acetylation of NF-κB-p65 by blocking phosphorylation of NF-κB-p65 at Ser(276) and also to disrupt the binding of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01389

    authors: Chen D,Zheng Z,Xiao B,Li W,Long M,Chen H,Li M,Rock DL,Hao W,Luo S

    更新日期:2016-09-13 00:00:00

  • Description and Comparative Genomics of Macrococcus caseolyticus subsp. hominis subsp. nov., Macrococcus goetzii sp. nov., Macrococcus epidermidis sp. nov., and Macrococcus bohemicus sp. nov., Novel Macrococci From Human Clinical Material With Virulence P

    abstract::The genus Macrococcus is a close relative of the genus Staphylococcus. Whilst staphylococci are widespread as human pathogens, macrococci have not yet been reported from human clinical specimens. Here we investigated Gram-positive and catalase-positive cocci recovered from human clinical material and identified as Mac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01178

    authors: Mašlaňová I,Wertheimer Z,Sedláček I,Švec P,Indráková A,Kovařovic V,Schumann P,Spröer C,Králová S,Šedo O,Krištofová L,Vrbovská V,Füzik T,Petráš P,Zdráhal Z,Ružičková V,Doškař J,Pantuček R

    更新日期:2018-06-13 00:00:00

  • Stair-Step Pattern of Soil Bacterial Diversity Mainly Driven by pH and Vegetation Types Along the Elevational Gradients of Gongga Mountain, China.

    abstract::Ecological understandings of soil bacterial community succession and assembly mechanism along elevational gradients in mountains remain not well understood. Here, by employing the high-throughput sequencing technique, we systematically examined soil bacterial diversity patterns, the driving factors, and community asse...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00569

    authors: Li J,Shen Z,Li C,Kou Y,Wang Y,Tu B,Zhang S,Li X

    更新日期:2018-03-27 00:00:00

  • The GC Content as a Main Factor Shaping the Amino Acid Usage During Bacterial Evolution Process.

    abstract::Understanding how proteins evolve is important, and the order of amino acids being recruited into the genetic codons was found to be an important factor shaping the amino acid composition of proteins. The latest work about the last universal common ancestor (LUCA) makes it possible to determine the potential factors s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02948

    authors: Du MZ,Zhang C,Wang H,Liu S,Wei W,Guo FB

    更新日期:2018-12-07 00:00:00

  • Viral Lysis of Photosynthesizing Microbes As a Mechanism for Calcium Carbonate Nucleation in Seawater.

    abstract::Removal of carbon through the precipitation and burial of calcium carbonate in marine sediments constitutes over 70% of the total carbon on Earth and is partitioned between coastal and pelagic zones. The precipitation of authigenic calcium carbonate in seawater, however, has been hotly debated because despite being in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01958

    authors: Lisle JT,Robbins LL

    更新日期:2016-12-09 00:00:00

  • Draft Genome Analysis Offers Insights Into the Mechanism by Which Streptomyces chartreusis WZS021 Increases Drought Tolerance in Sugarcane.

    abstract::Drought directly affects sugarcane production. Plant growth-promoting bacteria have gained attention as growth promoters of plants under abiotic stresses. The present study focused on genome assessment of the plant-beneficial endophyte Streptomyces chartreusis WZS021 and its vital role in sugarcane plants under drough...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03262

    authors: Wang Z,Solanki MK,Yu ZX,Yang LT,An QL,Dong DF,Li YR

    更新日期:2019-01-09 00:00:00

  • The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori.

    abstract::The human gastric lumen is one of the most hostile environments of the human body suspected to be sterile until the discovery of Helicobacter pylori (H.p.). State of the art next generation sequencing technologies multiply the knowledge on H.p. functional genomics as well as on the colonization of supposed sterile hum...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02508

    authors: Klymiuk I,Bilgilier C,Stadlmann A,Thannesberger J,Kastner MT,Högenauer C,Püspök A,Biowski-Frotz S,Schrutka-Kölbl C,Thallinger GG,Steininger C

    更新日期:2017-12-14 00:00:00

  • A Systematic Evaluation of the Two-Component Systems Network Reveals That ArlRS Is a Key Regulator of Catheter Colonization by Staphylococcus aureus.

    abstract::Two-component systems (TCS) are modular signal transduction pathways that allow cells to adapt to prevailing environmental conditions by modifying cellular physiology. Staphylococcus aureus has 16 TCSs to adapt to the diverse microenvironments encountered during its life cycle, including host tissues and implanted med...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00342

    authors: Burgui S,Gil C,Solano C,Lasa I,Valle J

    更新日期:2018-03-07 00:00:00

  • In-depth Profiling of MvfR-Regulated Small Molecules in Pseudomonas aeruginosa after Quorum Sensing Inhibitor Treatment.

    abstract::Pseudomonas aeruginosa is a Gram-negative bacterium, which causes opportunistic infections in immuno-compromised individuals. Due to its multiple resistances toward antibiotics, the development of new drugs is required. Interfering with Quorum Sensing (QS), a cell-to-cell communication system, has shown to be highly e...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00924

    authors: Allegretta G,Maurer CK,Eberhard J,Maura D,Hartmann RW,Rahme L,Empting M

    更新日期:2017-05-24 00:00:00

  • Enhanced Viability and Anti-rotavirus Effect of Bifidobacterium longum and Lactobacillus plantarum in Combination With Chlorella sorokiniana in a Dairy Product.

    abstract::Microalgae and probiotics such as Bifidobacterium and Lactobacillus genera are associated with human beneficial effects. The aim of this study was to evaluate the activity of Chlorella sorokiniana on Bifidobacterium longum and Lactobacillus plantarum viability in a dairy product (flan) and its microbial effect against...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00875

    authors: Cantú-Bernal S,Domínguez-Gámez M,Medina-Peraza I,Aros-Uzarraga E,Ontiveros N,Flores-Mendoza L,Gomez-Flores R,Tamez-Guerra P,González-Ochoa G

    更新日期:2020-05-12 00:00:00

  • Therapy for Mycobacterium kansasii Infection: Beyond 2018.

    abstract::The current standard of care therapy for pulmonary Mycobacterium kansasii infection is isoniazid (300 mg/day), rifampin (600 mg/day), and ethambutol (15 mg/kg/day) for 12 months after achieving sputum culture negativity. Rifampin is the key drug in this regimen. The contribution of isoniazid is unclear since its in vi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02271

    authors: DeStefano MS,Shoen CM,Cynamon MH

    更新日期:2018-09-24 00:00:00

  • Quantitative RT-PCR assay for high-throughput screening (HTS) of drugs against the growth of Cryptosporidium parvum in vitro.

    abstract::Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA. This approach displayed up to four orders of magnitude of linear dynamic range and was much less labor-intensive than the traditional microscopi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00991

    authors: Zhang H,Zhu G

    更新日期:2015-09-22 00:00:00

  • Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens.

    abstract::Desulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria is poorly understood. Here, we investigated Fe(III) reduction with lactate, a non-fermentable substrate, as the electron donor. Lactate consumption is concomi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00432

    authors: Dalla Vecchia E,Shao PP,Suvorova E,Chiappe D,Hamelin R,Bernier-Latmani R

    更新日期:2014-08-19 00:00:00

  • Peptidoglycan Muropeptides: Release, Perception, and Functions as Signaling Molecules.

    abstract::Peptidoglycan (PG) is an essential molecule for the survival of bacteria, and thus, its biosynthesis and remodeling have always been in the spotlight when it comes to the development of antibiotics. The peptidoglycan polymer provides a protective function in bacteria, but at the same time is continuously subjected to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00500

    authors: Irazoki O,Hernandez SB,Cava F

    更新日期:2019-03-28 00:00:00

  • Microbial Small Talk: Volatiles in Fungal-Bacterial Interactions.

    abstract::There is increasing evidence that volatile organic compounds (VOCs) play an important role in the interactions between fungi and bacteria, two major groups of soil inhabiting microorganisms. Yet, most of the research has been focused on effects of bacterial volatiles on suppression of plant pathogenic fungi whereas li...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01495

    authors: Schmidt R,Etalo DW,de Jager V,Gerards S,Zweers H,de Boer W,Garbeva P

    更新日期:2016-01-05 00:00:00

  • Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay.

    abstract::Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow im...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.596005

    authors: Shi L,Xu L,Xiao R,Zhou Z,Wang C,Wang S,Gu B

    更新日期:2020-11-06 00:00:00

  • Effects of Lactobacillus fermentum CQPC04 on Lipid Reduction in C57BL/6J Mice.

    abstract::Probiotics are functional foods that can effectively regulate lipid reduction and maintain body health. In this study, a strain of Lactobacillus fermentum CQPC04 (LF-CQPC04) isolated from traditional naturally fermented vegetables (Sichuan pickles) was studied, and its effects on lipid reduction in mice, as well as it...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.573586

    authors: Yi R,Tan F,Zhou X,Mu J,Li L,Du X,Yang Z,Zhao X

    更新日期:2020-09-03 00:00:00

  • Increased Denitrification Rates Associated with Shifts in Prokaryotic Community Composition Caused by Varying Hydrologic Connectivity.

    abstract::While modern developments in agriculture have allowed for increases in crop yields and rapid human population growth, they have also drastically altered biogeochemical cycles, including the biotransformation of nitrogen. Denitrification is a critical process performed by bacteria and fungi that removes nitrate in surf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02304

    authors: Tomasek A,Staley C,Wang P,Kaiser T,Lurndahl N,Kozarek JL,Hondzo M,Sadowsky MJ

    更新日期:2017-11-22 00:00:00

  • Differential Regulation of Myeloid-Derived Suppressor Cells by Candida Species.

    abstract::Myeloid-derived suppressor cells (MDSCs) are innate immune cells characterized by their ability to suppress T-cell responses. Recently, we demonstrated that the human-pathogenic fungi Candida albicans and Aspergillus fumigatus induced a distinct subset of neutrophilic MDSCs. To dissect Candida-mediated MDSC induction ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01624

    authors: Singh A,Lelis F,Braig S,Schäfer I,Hartl D,Rieber N

    更新日期:2016-10-13 00:00:00

  • Characterization of Chemosynthetic Microbial Mats Associated with Intertidal Hydrothermal Sulfur Vents in White Point, San Pedro, CA, USA.

    abstract::The shallow-sea hydrothermal vents at White Point (WP) in Palos Verdes on the southern California coast support microbial mats and provide easily accessed settings in which to study chemolithoautotrophic sulfur cycling. Previous studies have cultured sulfur-oxidizing bacteria from the WP mats; however, almost nothing ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01163

    authors: Miranda PJ,McLain NK,Hatzenpichler R,Orphan VJ,Dillon JG

    更新日期:2016-07-27 00:00:00

  • Next Generation Sequencing for Detection and Discovery of Plant Viruses and Viroids: Comparison of Two Approaches.

    abstract::Next generation sequencing (NGS) technologies are becoming routinely employed in different fields of virus research. Different sequencing platforms and sample preparation approaches, in the laboratories worldwide, contributed to a revolution in detection and discovery of plant viruses and viroids. In this work, we are...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01998

    authors: Pecman A,Kutnjak D,Gutiérrez-Aguirre I,Adams I,Fox A,Boonham N,Ravnikar M

    更新日期:2017-10-13 00:00:00

  • The Novel Anaerobiosis-Responsive Overlapping Gene ano Is Overlapping Antisense to the Annotated Gene ECs2385 of Escherichia coli O157:H7 Sakai.

    abstract::Current notion presumes that only one protein is encoded at a given bacterial genetic locus. However, transcription and translation of an overlapping open reading frame (ORF) of 186 bp length were discovered by RNAseq and RIBOseq experiments. This ORF is almost completely embedded in the annotated L,D-transpeptidase g...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00931

    authors: Hücker SM,Vanderhaeghen S,Abellan-Schneyder I,Scherer S,Neuhaus K

    更新日期:2018-05-14 00:00:00

  • Engineering nanoparticles to silence bacterial communication.

    abstract::The alarming spread of bacterial resistance to traditional antibiotics has warranted the study of alternative antimicrobial agents. Quorum sensing (QS) is a chemical cell-to-cell communication mechanism utilized by bacteria to coordinate group behaviors and establish infections. QS is integral to bacterial survival, a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00189

    authors: Miller KP,Wang L,Chen YP,Pellechia PJ,Benicewicz BC,Decho AW

    更新日期:2015-03-10 00:00:00