Quantitative RT-PCR assay for high-throughput screening (HTS) of drugs against the growth of Cryptosporidium parvum in vitro.

abstract：:Halophilic organisms inhabit hypersaline environments where the extreme ionic conditions and osmotic pressure have driven the evolution of molecular adaptation mechanisms. Understanding such mechanisms is limited by the common difficulties encountered in cultivating such organisms. Within the Euryarchaeota, for exampl...

journal_title：Frontiers in microbiology

authors： Gonzalez-Ordenes F,Cea PA,Fuentes-Ugarte N,Muñoz SM,Zamora RA,Leonardo D,Garratt RC,Castro-Fernandez V,Guixé V

更新日期：2018-06-26 00:00:00

abstract：:The current picture of human salmonellosis shows Salmonella Typhimurium and S. 4,[5],12:i:- as the most common serovars in Italy. The aims of this study were to investigate the genetic relationship between these serovars, as well as to test the possibility of inferring sources of human salmonellosis due to S. Typhimur...

journal_title：Frontiers in microbiology

authors： Barco L,Barrucci F,Cortini E,Ramon E,Olsen JE,Luzzi I,Lettini AA,Ricci A

更新日期：2015-04-27 00:00:00

abstract：:Proteus spp. are commensal Enterobacterales of the human digestive tract. At the same time, P. mirabilis is commonly involved in urinary tract infections (UTI). P. mirabilis is naturally resistant to several antibiotics including colistin and shows reduced susceptibility to imipenem. However higher levels of resistanc...

journal_title：Frontiers in microbiology

authors： Girlich D,Bonnin RA,Dortet L,Naas T

更新日期：2020-02-21 00:00:00

abstract：:Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) have important therapeutic and nutritional benefits in humans. In the biosynthesis pathways of these LC-PUFAs, omega-3 desaturase plays a critical role. In this study, we report a new omega-3 desaturase (PPD17) from Phytophthora parasitica. This desaturase shar...

journal_title：Frontiers in microbiology

authors： Tang X,Chen H,Mei T,Ge C,Gu Z,Zhang H,Chen YQ,Chen W

更新日期：2018-08-14 00:00:00

abstract：:Invertebrate immune priming is a growing field in immunology. This phenomenon refers to the ability of invertebrates to generate a more vigorous immune response to a second encounter with a specific pathogen and can occur within and across generations. Although the precise mechanism has not been elucidated, it has bee...

journal_title：Frontiers in microbiology

authors： Castro-Vargas C,Linares-López C,López-Torres A,Wrobel K,Torres-Guzmán JC,Hernández GA,Wrobel K,Lanz-Mendoza H,Contreras-Garduño J

更新日期：2017-03-28 00:00:00

abstract：:Perennial ryegrass (Lolium perenne) is an important forage grass with high yield and superior quality in temperate regions which is widely used in parks, sport field, and other places. However, perennial ryegrass is moderately tolerant to salinity stress compared to other commercial cultivars and salt stress reduces t...

journal_title：Frontiers in microbiology

authors： Li X,Han S,Wang G,Liu X,Amombo E,Xie Y,Fu J

更新日期：2017-09-04 00:00:00

abstract：:Microbial community composition and stability affect pollutant removal for biological/granular activated carbon (BAC/GAC) processes. Here, we pre-loaded the organic carbon substrates sucrose, lactose, and Lysogeny Broth (LB) medium onto new GAC prior to use and then tested whether this substrate pre-loading promoted d...

journal_title：Frontiers in microbiology

authors： Qin W,Hammes F

更新日期：2021-01-12 00:00:00

abstract：:Background/objectives: Whole genome sequencing (WGS) has proven to be a powerful subtyping tool for foodborne pathogenic bacteria like L. monocytogenes. The interests of genome-scale analysis for national surveillance, outbreak detection or source tracking has been largely documented. The genomic data however can be e...

journal_title：Frontiers in microbiology

authors： Henri C,Leekitcharoenphon P,Carleton HA,Radomski N,Kaas RS,Mariet JF,Felten A,Aarestrup FM,Gerner Smidt P,Roussel S,Guillier L,Mistou MY,Hendriksen RS

更新日期：2017-11-29 00:00:00

abstract：:In the present work, loop-mediated isothermal amplification (LAMP) and hyperbranched rolling circle amplification (HRCA) methods were developed to detect and distinguish different lethal Amanita species. Specific LAMP primers and HRCA padlock probes for species-specific identification and a set of universal LAMP prime...

journal_title：Frontiers in microbiology

authors： He Z,Su Y,Li S,Long P,Zhang P,Chen Z

更新日期：2019-07-03 00:00:00

abstract：:Streptococcus pneumoniae colonizes the upper airways of children and the elderly. Colonization progresses to persistent carriage when S. pneumoniae forms biofilms, a feature required for the development of pneumococcal disease. Nasopharyngeal biofilms are structured with a matrix that includes extracellular DNA (eDNA)...

journal_title：Frontiers in microbiology

authors： Angulo-Zamudio UA,Vidal JE,Nazmi K,Bolscher JGM,Leon-Sicairos C,Antezana BS,Canizalez-Roman A,León-Sicairos N

更新日期：2019-10-18 00:00:00

abstract：:Shewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble substrate by acting as...

journal_title：Frontiers in microbiology

authors： Arinda T,Philipp LA,Rehnlund D,Edel M,Chodorski J,Stöckl M,Holtmann D,Ulber R,Gescher J,Sturm-Richter K

更新日期：2019-02-05 00:00:00

abstract：:Current knowledge about the relationships between ruminal bacterial communities and metabolite profiles in the yak rumen is limited. This is due to differences in the nutritional and metabolic features between yak and other ordinary cattle combined with difficulties associated with farm-based research and a lack of te...

journal_title：Frontiers in microbiology

authors： Liu C,Wu H,Liu S,Chai S,Meng Q,Zhou Z

更新日期：2019-05-22 00:00:00

abstract：:Woodchip bioreactor technology removes nitrate from agricultural subsurface drainage by using denitrifying microorganisms. Although woodchip bioreactors have demonstrated success in many field locations, low water temperature can significantly limit bioreactor efficiency and performance. To improve bioreactor performa...

journal_title：Frontiers in microbiology

authors： Jang J,Anderson EL,Venterea RT,Sadowsky MJ,Rosen CJ,Feyereisen GW,Ishii S

更新日期：2019-04-02 00:00:00

abstract：:Agricultural ponds have a great potential as a means of capture and storage of water for irrigation. However, pond topography (small size, shallow depth) leaves them susceptible to environmental, agricultural, and anthropogenic exposures that may influence microbial dynamics. Therefore, the aim of this project was to ...

journal_title：Frontiers in microbiology

authors： Chopyk J,Allard S,Nasko DJ,Bui A,Mongodin EF,Sapkota AR

更新日期：2018-04-24 00:00:00

abstract：:Despite their potential importance as analogs of primitive microbial metabolisms, the knowledge of the structure and functioning of the deep ecosystems associated with serpentinizing environments is hampered by the lack of accessibility to relevant systems. These hyperalkaline environments are depleted in dissolved in...

journal_title：Frontiers in microbiology

authors： Pisapia C,Gérard E,Gérard M,Lecourt L,Lang SQ,Pelletier B,Payri CE,Monnin C,Guentas L,Postec A,Quéméneur M,Erauso G,Ménez B

更新日期：2017-01-31 00:00:00

abstract：:Coastal eutrophication is a key driver of shifts in bacterial communities on coral reefs. With fringing and patch reefs at varying distances from the coast the Spermonde Archipelago in southern Sulawesi, Indonesia offers ideal conditions to study the effects of coastal eutrophication along a spatially defined gradient...

journal_title：Frontiers in microbiology

authors： Kegler HF,Lukman M,Teichberg M,Plass-Johnson J,Hassenrück C,Wild C,Gärdes A

更新日期：2017-04-20 00:00:00

abstract：:Acinetobacter baumannii, a Gram-negative opportunistic pathogen, is known to cause multidrug resistant infections. This organism has primarily been isolated from clinical environments and its environmental reservoirs remain largely unknown. In the present study, we recovered seven isolates of A. baumannii growing unde...

journal_title：Frontiers in microbiology

authors： Fernando DM,Khan IU,Patidar R,Lapen DR,Talbot G,Topp E,Kumar A

更新日期：2016-11-18 00:00:00

abstract：:Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathoge...

journal_title：Frontiers in microbiology

authors： Rothrock MJ Jr,Feye KM,Kim SA,Park SH,Locatelli A,Hiett KL,Gamble J,Sellers H,Ricke SC

更新日期：2018-11-02 00:00:00

abstract：:Aeolian prokaryotic communities (APC) are important components of bioaerosols that are transported freely or attached to dust particles suspended in the atmosphere. Terrestrial and marine ecosystems are known to release and receive significant prokaryote loads into and from the surrounded atmospheric air. However, com...

journal_title：Frontiers in microbiology

authors： Aalismail NA,Díaz-Rúa R,Ngugi DK,Cusack M,Duarte CM

更新日期：2020-11-12 00:00:00

abstract：:In this study we addressed the question how a mevalonate (MVA)-auxotrophic Staphylococcus aureusΔmvaS mutant can revert to prototrophy. This mutant couldn't grow in the absence of MVA. However, after a long lag-phase of 4-6 days the mutant adapted from auxotrophic to prototrophic phenotype. During that time, it acquir...

journal_title：Frontiers in microbiology

authors： Reichert S,Ebner P,Bonetti EJ,Luqman A,Nega M,Schrenzel J,Spröer C,Bunk B,Overmann J,Sass P,François P,Götz F

更新日期：2018-07-12 00:00:00

abstract：:Approximately four billion years ago, the first microorganisms to thrive on earth were anaerobic chemoautotrophic thermophiles, a specific group of extremophiles that survive and operate at temperatures ∼50 - 125°C and do not use molecular oxygen (O2) for respiration. Instead, these microorganisms performed respiratio...

journal_title：Frontiers in microbiology

authors： Lusk BG

更新日期：2019-04-26 00:00:00

abstract：:Ionic liquids discovery has celebrated 100 years. They consist solely of ions, one of which is typically organic and asymmetrical. Remarkable physical and chemical properties stirred their use as alternative solvents in many chemical processes. The recent demonstration of their occurrence in nature might boost their i...

journal_title：Frontiers in microbiology

authors： Hartmann DO,Petkovic M,Silva Pereira C

更新日期：2016-02-08 00:00:00

abstract：:Cactaceae represents one of the most species-rich families of succulent plants native to arid and semi-arid ecosystems, yet the associations Cacti establish with microorganisms and the rules governing microbial community assembly remain poorly understood. We analyzed the composition, diversity, and factors influencing...

journal_title：Frontiers in microbiology

authors： Fonseca-García C,Coleman-Derr D,Garrido E,Visel A,Tringe SG,Partida-Martínez LP

更新日期：2016-02-12 00:00:00

abstract：:Pharmaceutical contaminants (PCs) have been recognized as emerging contaminants causing unexpected consequences to environment and humans. There is an urgent need for development of efficient technologies to treat these PCs from water. The current study has investigated the removal capacity of a green microalgal speci...

journal_title：Frontiers in microbiology

authors： Xiong JQ,Cui P,Ru S

更新日期：2020-11-03 00:00:00

abstract：:Iron is an essential but, in excess, toxic nutrient. Therefore, fungi evolved fine-tuned mechanisms for uptake and storage of iron, such as the production of siderophores (low-molecular mass iron-specific chelators). In Aspergillus fumigatus, iron starvation causes extensive transcriptional remodeling involving two ce...

journal_title：Frontiers in microbiology

authors： Haas H

更新日期：2012-02-06 00:00:00

abstract：:Edaphic cyanobacteria and algae have been extensively studied in dryland soils because they play key roles in the formation of biological soil crusts and the stabilization of soil surfaces. Yet, in temperate agricultural crop soils, little is understood about the functional significance of indigenous photosynthetic mi...

journal_title：Frontiers in microbiology

authors： Crouzet O,Consentino L,Pétraud JP,Marrauld C,Aguer JP,Bureau S,Le Bourvellec C,Touloumet L,Bérard A

更新日期：2019-06-14 00:00:00

abstract：:Fecal microbiota transplants (FMT) may be used to improve chicken's feed efficiency (FE) via modulation of the intestinal microbiota and microbe-host signaling. This study investigated the effect of the administration of FMT from highly feed efficient donors early in life on the jejunal and cecal microbiota, visceral ...

journal_title：Frontiers in microbiology

authors： Metzler-Zebeli BU,Siegerstetter SC,Magowan E,Lawlor PG,O Connell NE,Zebeli Q

更新日期：2019-07-09 00:00:00

abstract：:Plant rhizospheres encompass a dynamic zone of interactions between microorganisms and their respective plant hosts. For decades, researchers have worked to understand how these complex interactions influence different aspects of plant growth, development, and evolution. Studies of plant-microbial interactions in the ...

journal_title：Frontiers in microbiology

authors： Garcia J,Kao-Kniffin J

更新日期：2018-07-11 00:00:00

abstract：:Extremely cold environments are a challenge for all organisms. They are mostly inhabited by psychrophilic and psychrotolerant bacteria, which employ various strategies to cope with the cold. Such harsh environments are often highly vulnerable to the influence of external factors and may undergo frequent dynamic change...

journal_title：Frontiers in microbiology

authors： Dziewit L,Bartosik D

更新日期：2014-11-06 00:00:00

abstract：:A multidisciplinary, collaborative project was conducted to determine the prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan and ascertain its Public Health Significance. Using a grid-based sampling strategy, soil samples (n = 145) were collected from villages (n = 29, 5 sample...

journal_title：Frontiers in microbiology

authors： Shabbir MZ,Jamil T,Ali AA,Ahmad A,Naeem M,Chaudhary MH,Bilal M,Ali MA,Muhammad K,Yaqub T,Bano A,Mirza AI,Shabbir MA,McVey WR,Patel K,Francesconi S,Jayarao BM,Rabbani M

更新日期：2015-09-10 00:00:00