Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens.

Abstract:

:Desulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria is poorly understood. Here, we investigated Fe(III) reduction with lactate, a non-fermentable substrate, as the electron donor. Lactate consumption is concomitant to Fe(III) reduction, but does not support significant growth, suggesting that little energy can be conserved from this process and that it may occur fortuitously. D. reducens can reduce both soluble [Fe(III)-citrate] and insoluble (hydrous ferric oxide, HFO) Fe(III). Because physically inaccessible HFO was not reduced, we concluded that reduction requires direct contact under these experimental conditions. This implies the presence of a surface exposed reductase capable of transferring electrons from the cell to the extracellular electron acceptor. With the goal of characterizing the role of surface proteins in D. reducens and of identifying candidate Fe(III) reductases, we carried out an investigation of the surface proteome (surfaceome) of D. reducens. Cell surface exposed proteins were extracted by trypsin cell shaving or by lysozyme treatment, and analyzed by liquid chromatography-tandem mass spectrometry. This investigation revealed that the surfaceome fulfills many functions, including solute transport, protein export, maturation and hydrolysis, peptidoglycan synthesis and modification, and chemotaxis. Furthermore, a few redox-active proteins were identified. Among these, three are putatively involved in Fe(III) reduction, i.e., a membrane-bound hydrogenase 4Fe-4S cluster subunit (Dred_0462), a heterodisulfide reductase subunit A (Dred_0143) and a protein annotated as alkyl hydroperoxide reductase but likely functioning as a thiol-disulfide oxidoreductase (Dred_1533).

journal_name

Front Microbiol

authors

Dalla Vecchia E,Shao PP,Suvorova E,Chiappe D,Hamelin R,Bernier-Latmani R

doi

10.3389/fmicb.2014.00432

subject

Has Abstract

pub_date

2014-08-19 00:00:00

pages

432

issn

1664-302X

journal_volume

5

pub_type

杂志文章
  • A hundred years of activated sludge: time for a rethink.

    abstract::Biological wastewater treatment plants (BWWTPs) based on the activated sludge (AS) process have dramatically improved worldwide water sanitation despite increased urbanization and industrialization. However, current AS-based operations are considered economically and environmentally unsustainable. In this Perspective,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00047

    authors: Sheik AR,Muller EE,Wilmes P

    更新日期:2014-03-03 00:00:00

  • Chloroplast symbiosis in a marine ciliate: ecophysiology and the risks and rewards of hosting foreign organelles.

    abstract::Simultaneous use of both heterotrophic and autotrophic metabolism ("mixotrophy") is common among protists. Strombidium rassoulzadegani is a planktonic mixotrophic marine ciliate that saves chloroplasts from its algal food and obtains a nutritional subsidy via photosynthesis. Cultures from the northeast, northwest, and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00321

    authors: McManus GB,Schoener DM,Haberlandt K

    更新日期:2012-09-04 00:00:00

  • Identification and Characterization of a Novel Basic Helix-Loop-Helix Transcription Factor of Phospholipid Synthesis Regulation in Aspergillus niger.

    abstract::The synthesis of phospholipids relies on a sort of genes, whose promoter regions contain inositol-sensitive upstream activation sequence (UASINO) and are regulated by the basic helix-loop-helix (bHLH)-type ino2/ino4 transcription factor (TF) pair. Ten putative bHLH TFs have been found through whole genome sequencing o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02985

    authors: Dong H,Yu D,Wang B,Pan L

    更新日期:2020-01-09 00:00:00

  • Metagenomics-Based, Strain-Level Analysis of Escherichia coli From a Time-Series of Microbiome Samples From a Crohn's Disease Patient.

    abstract::Dysbiosis of the gut microbiome, including elevated abundance of putative leading bacterial triggers such as E. coli in inflammatory bowel disease (IBD) patients, is of great interest. To date, most E. coli studies in IBD patients are focused on clinical isolates, overlooking their relative abundances and turnover ove...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02559

    authors: Fang X,Monk JM,Nurk S,Akseshina M,Zhu Q,Gemmell C,Gianetto-Hill C,Leung N,Szubin R,Sanders J,Beck PL,Li W,Sandborn WJ,Gray-Owen SD,Knight R,Allen-Vercoe E,Palsson BO,Smarr L

    更新日期:2018-10-30 00:00:00

  • Toxicological and Medical Aspects of Aspergillus-Derived Mycotoxins Entering the Feed and Food Chain.

    abstract::Due to Earth's changing climate, the ongoing and foreseeable spreading of mycotoxigenic Aspergillus species has increased the possibility of mycotoxin contamination in the feed and food production chain. These harmful mycotoxins have aroused serious health and economic problems since their first appearance. The most p...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.02908

    authors: Ráduly Z,Szabó L,Madar A,Pócsi I,Csernoch L

    更新日期:2020-01-09 00:00:00

  • Inactivation Effect of Violet and Blue Light on ESKAPE Pathogens and Closely Related Non-pathogenic Bacterial Species - A Promising Tool Against Antibiotic-Sensitive and Antibiotic-Resistant Microorganisms.

    abstract::Due to the globally observed increase in antibiotic resistance of bacterial pathogens and the simultaneous decline in new antibiotic developments, the need for alternative inactivation approaches is growing. This is especially true for the treatment of infections with the problematic ESKAPE pathogens, which include En...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.612367

    authors: Hoenes K,Bauer R,Meurle T,Spellerberg B,Hessling M

    更新日期:2021-01-13 00:00:00

  • May the Phage be With You? Prophage-Like Elements in the Genomes of Soft Rot Pectobacteriaceae: Pectobacterium spp. and Dickeya spp.

    abstract::Soft Rot Pectobacteriaceae (SRP; Pectobacterium spp. and Dickeya spp., formerly known as pectinolytic Erwinia spp.) are necrotrophic bacterial pathogens infecting a large number of plant species worldwide, including agriculturally-important crops. Despite the SRP importance in agriculture, little is known about the ba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00138

    authors: Czajkowski R

    更新日期:2019-02-14 00:00:00

  • Diversity-Stability Dynamics of the Amphibian Skin Microbiome and Susceptibility to a Lethal Viral Pathogen.

    abstract::Variation among animals in their host-associated microbial communities is increasingly recognized as a key determinant of important life history traits including growth, metabolism, and resistance to disease. Quantitative estimates of the factors shaping the stability of host microbiomes over time at the individual le...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02883

    authors: Harrison XA,Price SJ,Hopkins K,Leung WTM,Sergeant C,Garner TWJ

    更新日期:2019-12-20 00:00:00

  • Plant and Microbial Responses to Repeated Cu(OH)2 Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.

    abstract::The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realisti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01769

    authors: Simonin M,Colman BP,Tang W,Judy JD,Anderson SM,Bergemann CM,Rocca JD,Unrine JM,Cassar N,Bernhardt ES

    更新日期:2018-07-31 00:00:00

  • Quorum Sensing Inhibiting Activity of Streptomyces coelicoflavus Isolated from Soil.

    abstract::Quorum sensing (QS) systems communicate bacterial population and stimulate microbial pathogenesis through signaling molecules. Inhibition of QS signals potentially suppresses microbial infections. Antimicrobial properties of Streptomyces have been extensively studied, however, less is known about quorum sensing inhibi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00659

    authors: Hassan R,Shaaban MI,Abdel Bar FM,El-Mahdy AM,Shokralla S

    更新日期:2016-05-13 00:00:00

  • Biophysical Characterization of Membrane Phase Transition Profiles for the Discrimination of Outer Membrane Vesicles (OMVs) From Escherichia coli Grown at Different Temperatures.

    abstract::Dynamic Light Scattering (DLS), Small Angle X-ray Scattering (SAXS) and Transmission Electron Microscopy (TEM) are physical techniques widely employed to characterize the morphology and the structure of vesicles such as liposomes or human extracellular vesicles (exosomes). Bacterial extracellular vesicles are similar ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00290

    authors: Sarra A,Celluzzi A,Bruno SP,Ricci C,Sennato S,Ortore MG,Casciardi S,Del Chierico F,Postorino P,Bordi F,Masotti A

    更新日期:2020-02-27 00:00:00

  • Different Transcriptional Responses from Slow and Fast Growth Rate Strains of Listeria monocytogenes Adapted to Low Temperature.

    abstract::Listeria monocytogenes has become one of the principal foodborne pathogens worldwide. The capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of L. monocytogenes with different growth capacities at low temperat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00229

    authors: Cordero N,Maza F,Navea-Perez H,Aravena A,Marquez-Fontt B,Navarrete P,Figueroa G,González M,Latorre M,Reyes-Jara A

    更新日期:2016-03-01 00:00:00

  • Effect of Dietary Forage to Concentrate Ratios on Dynamic Profile Changes and Interactions of Ruminal Microbiota and Metabolites in Holstein Heifers.

    abstract::A better understanding of global ruminal microbiota and metabolites under extensive feeding conditions is a prerequisite for optimizing rumen function and improving ruminant feed efficiency. Furthermore, the gap between the information on the ruminal microbiota and metabolites needs to be bridged. The aim of this stud...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02206

    authors: Zhang J,Shi H,Wang Y,Li S,Cao Z,Ji S,He Y,Zhang H

    更新日期:2017-11-09 00:00:00

  • FDA Approved Drug Library Screening Identifies Robenidine as a Repositionable Antifungal.

    abstract::Due to the increasing prevalence of pathogenic fungal infections, the emergence of antifungal resistant clinical isolates worldwide, and the limited arsenal of available antifungals, developing new antifungal strategies is imperative. In this study, we screened a library of 1068 FDA-approved drugs to identify hits tha...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00996

    authors: Mei Y,Jiang T,Zou Y,Wang Y,Zhou J,Li J,Liu L,Tan J,Wei L,Li J,Dai H,Peng Y,Zhang L,Lopez-Ribot JL,Shapiro RS,Chen C,Liu NN,Wang H

    更新日期:2020-06-03 00:00:00

  • Response to Trypanosoma cruzi by Human Blood Cells Enriched with Dentritic Cells Is Controlled by Cyclooxygenase-2 Pathway.

    abstract::Chagas disease (Cd) or American human trypanosomiasis is caused by Trypanosoma cruzi and affects ~7 million people, mostly in Latin America. The infective trypomastigote forms of the parasite can invade several human blood cell populations, including monocytes and dendritic cells (DC). Although these cells display a w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02020

    authors: Lonien SCH,Malvezi AD,Suzukawa HT,Yamauchi LM,Yamada-Ogatta SF,Rizzo LV,Bordignon J,Pinge-Filho P

    更新日期:2017-10-25 00:00:00

  • The Antifungal Plant Defensin HsAFP1 from Heuchera sanguinea Induces Apoptosis in Candida albicans.

    abstract::Plant defensins are active against plant and human pathogenic fungi (such as Candida albicans) and baker's yeast. However, they are non-toxic to human cells, providing a possible source for treatment of fungal infections. In this study, we characterized the mode of action of the antifungal plant defensin HsAFP1 from c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00047

    authors: Aerts AM,Bammens L,Govaert G,Carmona-Gutierrez D,Madeo F,Cammue BP,Thevissen K

    更新日期:2011-03-16 00:00:00

  • Pseudomonas aeruginosa Quorum-Sensing and Type VI Secretion System Can Direct Interspecific Coexistence During Evolution.

    abstract::It is reported that a wide range of bacterial infections are polymicrobial, and the members in a local microcommunity can influence the growth of neighbors through physical and chemical interactions. Pseudomonas aeruginosa is an important opportunistic pathogen that normally causes a variety of acute and chronic infec...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02287

    authors: Zhao K,Du L,Lin J,Yuan Y,Wang X,Yue B,Wang X,Guo Y,Chu Y,Zhou Y

    更新日期:2018-10-11 00:00:00

  • Contrasting Soil Bacterial Community, Diversity, and Function in Two Forests in China.

    abstract::Bacteria are the highest abundant microorganisms in the soil. To investigate bacteria community structures, diversity, and functions, contrasting them in four different seasons all the year round with/within two different forest type soils of China. We analyzed soil bacterial community based on 16S rRNA gene sequencin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01693

    authors: Wei H,Peng C,Yang B,Song H,Li Q,Jiang L,Wei G,Wang K,Wang H,Liu S,Liu X,Chen D,Li Y,Wang M

    更新日期:2018-07-31 00:00:00

  • Regulation of the ER Stress Response by the Ion Channel Activity of the Infectious Bronchitis Coronavirus Envelope Protein Modulates Virion Release, Apoptosis, Viral Fitness, and Pathogenesis.

    abstract::Coronavirus (CoV) envelope (E) protein is a small structural protein critical for virion morphogenesis and release. The recently characterized E protein ion channel activity (EIC) has also been implicated in modulating viral pathogenesis. In this study, we used infectious bronchitis coronavirus (IBV) as a model to stu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03022

    authors: Li S,Yuan L,Dai G,Chen RA,Liu DX,Fung TS

    更新日期:2020-01-24 00:00:00

  • Detection and Genetic Characterization of Atypical Porcine Pestivirus in Piglets With Congenital Tremors in Southern China.

    abstract::Atypical porcine pestivirus (APPV) is an RNA virus newly discovered from swine in Asia, Europe, and the Americas. This novel virus has been confirmed as the cause of congenital tremor (CT) in piglets, which causes extensive economic losses to the swine industry. To investigate the genetic diversity and evolutionary re...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01406

    authors: Xie Y,Wang X,Su D,Feng J,Wei L,Cai W,Li J,Lin S,Yan H,He D

    更新日期:2019-06-20 00:00:00

  • Marinobacter subterrani, a genetically tractable neutrophilic Fe(II)-oxidizing strain isolated from the Soudan Iron Mine.

    abstract::We report the isolation, characterization, and development of a robust genetic system for a halophilic, Fe(II)-oxidizing bacterium isolated from a vertical borehole originating 714 m below the surface located in the Soudan Iron Mine in northern Minnesota, USA. Sequence analysis of the 16S rRNA gene places the isolate ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00719

    authors: Bonis BM,Gralnick JA

    更新日期:2015-07-16 00:00:00

  • Designing and Engineering Methylorubrum extorquens AM1 for Itaconic Acid Production.

    abstract::Methylorubrum extorquens (formerly Methylobacterium extorquens) AM1 is a methylotrophic bacterium with a versatile lifestyle. Various carbon sources including acetate, succinate and methanol are utilized by M. extorquens AM1 with the latter being a promising inexpensive substrate for use in the biotechnology industry....

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01027

    authors: Lim CK,Villada JC,Chalifour A,Duran MF,Lu H,Lee PKH

    更新日期:2019-05-09 00:00:00

  • Understanding the Effects of High Pressure on Bacterial Spores Using Synchrotron Infrared Spectroscopy.

    abstract::Bacterial spores are extremely resistant life-forms that play an important role in food spoilage and foodborne disease. The return of spores to a vegetative cell state is a three-step process, these being activation, germination, and emergence. High-pressure (HP) processing is known to induce germination in part of th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03122

    authors: Modugno C,Peltier C,Simonin H,Dujourdy L,Capitani F,Sandt C,Perrier-Cornet JM

    更新日期:2020-01-31 00:00:00

  • High-Throughput Sequence Analyses of Bacterial Communities and Multi-Mycotoxin Profiling During Processing of Different Formulations of Kunu, a Traditional Fermented Beverage.

    abstract::Kunu is a traditional fermented single or mixed cereals-based beverage popularly consumed in many parts of West Africa. Presently, the bacterial community and mycotoxin contamination profiles during processing of various kunu formulations have never been comprehensively studied. This study, therefore, investigated the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03282

    authors: Ezekiel CN,Ayeni KI,Ezeokoli OT,Sulyok M,van Wyk DAB,Oyedele OA,Akinyemi OM,Chibuzor-Onyema IE,Adeleke RA,Nwangburuka CC,Hajšlová J,Elliott CT,Krska R

    更新日期:2019-01-09 00:00:00

  • Diversity and Activity of Alternative Nitrogenases in Sequenced Genomes and Coastal Environments.

    abstract::The nitrogenase enzyme, which catalyzes the reduction of N2 gas to NH4+, occurs as three separate isozyme that use Mo, Fe-only, or V. The majority of global nitrogen fixation is attributed to the more efficient 'canonical' Mo-nitrogenase, whereas Fe-only and V-('alternative') nitrogenases are often considered 'backup'...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00267

    authors: McRose DL,Zhang X,Kraepiel AM,Morel FM

    更新日期:2017-02-28 00:00:00

  • MALDI-TOF MS Affords Discrimination of Deinococcus aquaticus Isolates Obtained From Diverse Biofilm Habitats.

    abstract::Matrix-assisted Laser Desorption Ionization-Time of Flight Mass Spectroscopy (MALDI-TOF MS) has been used routinely over the past decade in clinical microbiology laboratories to rapidly characterize diverse microorganisms of medical importance both at the genus and species levels. Currently, there is keen interest in ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02442

    authors: Tuohy JM,Mueller-Spitz SR,Albert CM,Scholz-Ng SE,Wall ME,Noutsios GT,Gutierrez AJ,Sandrin TR

    更新日期:2018-10-15 00:00:00

  • Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms.

    abstract::Benthic diatoms are dominant primary producers in intertidal mudflats and constitute a major source of organic carbon to consumers and decomposers residing within these ecosystems. They typically form biofilms whose species richness, community composition and productivity can vary in response to environmental drivers ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01255

    authors: Koedooder C,Stock W,Willems A,Mangelinckx S,De Troch M,Vyverman W,Sabbe K

    更新日期:2019-06-05 00:00:00

  • Resistance to Biocides in Listeria monocytogenes Collected in Meat-Processing Environments.

    abstract::The emergence of microorganisms exerting resistance to biocides is a challenge to meat-processing environments. Bacteria can be intrinsically resistant to biocides but resistance can also be acquired by adaptation to their sub-lethal concentrations. Moreover, the presence of biocide resistance determinants, which is c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01627

    authors: Conficoni D,Losasso C,Cortini E,Di Cesare A,Cibin V,Giaccone V,Corno G,Ricci A

    更新日期:2016-10-19 00:00:00

  • Macaque-tropic human immunodeficiency virus type 1: breaking out of the host restriction factors.

    abstract::Macaque monkeys serve as important animal models for understanding the pathogenesis of lentiviral infections. Since human immunodeficiency virus type 1 (HIV-1) hardly replicates in macaque cells, simian immunodeficiency virus (SIV) or chimeric viruses between HIV-1 and SIV (SHIV) have been used as challenge viruses in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00187

    authors: Saito A,Akari H

    更新日期:2013-07-09 00:00:00

  • Transcriptomics Indicates Active and Passive Metronidazole Resistance Mechanisms in Three Seminal Giardia Lines.

    abstract::Giardia duodenalis is an intestinal parasite that causes 200-300 million episodes of diarrhoea annually. Metronidazole (Mtz) is a front-line anti-giardial, but treatment failure is common and clinical resistance has been demonstrated. Mtz is thought to be activated within the parasite by oxidoreductase enzymes, and to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00398

    authors: Ansell BR,Baker L,Emery SJ,McConville MJ,Svärd SG,Gasser RB,Jex AR

    更新日期:2017-03-17 00:00:00