The Human Gastric Microbiome Is Predicated upon Infection with Helicobacter pylori.

Abstract:

:The human gastric lumen is one of the most hostile environments of the human body suspected to be sterile until the discovery of Helicobacter pylori (H.p.). State of the art next generation sequencing technologies multiply the knowledge on H.p. functional genomics as well as on the colonization of supposed sterile human environments like the gastric habitat. Here we studied in a prospective, multicenter, clinical trial the 16S rRNA gene amplicon based bacterial microbiome in a total of 30 homogenized and frozen gastric biopsy samples from eight geographic locations. The evaluation of the samples for H.p. infection status was done by histopathology and a specific PCR assay. CagA status was determined by a CagA-specific PCR assay. Patients were grouped accordingly as H.p.-negative, H.p.-positive but CagA-negative and H.p.-positive and CagA-positive (n = 10, respectively). Here we show that H.p. infection of the gastric habitat dominates the gastric microbiota in most patients and is associated with a significant decrease of the microbial alpha diversity from H.p. negative to H.p. positive with CagA as a considerable factor. The genera Actinomyces, Granulicatella, Veillonella, Fusobacterium, Neisseria, Helicobacter, Streptococcus, and Prevotella are significantly different between the H.p.-positive and H.p.-negative sample groups. Differences in microbiota found between CagA-positive and CagA-negative patients were not statistically significant and need to be re-evaluated in larger sample cohorts. In conclusion, H.p. infection dominates the gastric microbiome in a multicentre cohort of patients with varying diagnoses.

journal_name

Front Microbiol

authors

Klymiuk I,Bilgilier C,Stadlmann A,Thannesberger J,Kastner MT,Högenauer C,Püspök A,Biowski-Frotz S,Schrutka-Kölbl C,Thallinger GG,Steininger C

doi

10.3389/fmicb.2017.02508

subject

Has Abstract

pub_date

2017-12-14 00:00:00

pages

2508

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • A Bioguided Approach for the Screening of Antibacterial Compounds Isolated From the Hydroalcoholic Extract of the Native Brazilian Bee's Propolis Using Mollicutes as a Model.

    abstract::Nature is a vast source of medicinal substances, including propolis, which has been extensively investigated. Propolis is a resinous substance produced by bees from the exudates of plants that they collect and modify in their jaws; it is a rich and complex matrix with secondary metabolites of diverse botanical origins...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00558

    authors: Hochheim S,Pacassa Borges P,Boeder AM,Scharf DR,Simionatto EL,Yamanaka CN,Alberton MD,Guedes A,de Cordova CMM

    更新日期:2020-04-07 00:00:00

  • Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection.

    abstract::Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03147

    authors: Lukianova AA,Shneider MM,Evseev PV,Shpirt AM,Bugaeva EN,Kabanova AP,Obraztsova EA,Miroshnikov KK,Senchenkova SN,Shashkov AS,Toschakov SV,Knirel YA,Ignatov AN,Miroshnikov KA

    更新日期:2020-01-23 00:00:00

  • Insights in the Global Genetics and Gut Microbiome of Black Soldier Fly, Hermetia illucens: Implications for Animal Feed Safety Control.

    abstract::The utilization of the black soldier fly (BSF) Hermetia illucens L. for recycling organic waste into high-quality protein and fat biomass for animal feeds has gained momentum worldwide. However, information on the genetic diversity and environmental implications on safety of the larvae is limited. This study delineate...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01538

    authors: Khamis FM,Ombura FLO,Akutse KS,Subramanian S,Mohamed SA,Fiaboe KKM,Saijuntha W,Van Loon JJA,Dicke M,Dubois T,Ekesi S,Tanga CM

    更新日期:2020-07-07 00:00:00

  • Molecular Characteristics of Burkholderia pseudomallei Collected From Humans in Hainan, China.

    abstract::Melioidosis is a common infectious disease in Southeast Asia and Northern Australia. In Hainan, several cases have been reported, but no systematic study has yet been done on the molecular epidemiology profiles of the organism. An investigation of the molecular epidemiology links and population structure of Burkholder...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00778

    authors: Zhu X,Chen H,Li S,Wang LC,Wu DR,Wang XM,Chen RS,Li ZJ,Liu ZG

    更新日期:2020-05-07 00:00:00

  • Selection of Competitive and Efficient Rhizobia Strains for White Clover.

    abstract::The practice of inoculating forage legumes with rhizobia strains is widespread. It is assumed that the inoculated strain determines the performance of the symbiosis and nitrogen fixation rates. However, native-naturalized strains can be competitive, and actual nodule occupancy is often scarcely investigated. In conseq...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00768

    authors: Irisarri P,Cardozo G,Tartaglia C,Reyno R,Gutiérrez P,Lattanzi FA,Rebuffo M,Monza J

    更新日期:2019-04-23 00:00:00

  • Cell aggregation promotes pyoverdine-dependent iron uptake and virulence in Pseudomonas aeruginosa.

    abstract::In Pseudomonas aeruginosa the Gac signaling system and the second messenger cyclic diguanylate (c-di-GMP) participate in the control of the switch between planktonic and biofilm lifestyles, by regulating the production of the two exopolysaccharides Pel and Psl. The Gac and c-di-GMP regulatory networks also coordinatel...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00902

    authors: Visaggio D,Pasqua M,Bonchi C,Kaever V,Visca P,Imperi F

    更新日期:2015-08-28 00:00:00

  • Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect.

    abstract::This study evaluates the antibacterial activity of the Copaifera duckei Dwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00201

    authors: Abrão F,Alves JA,Andrade G,de Oliveira PF,Ambrósio SR,Veneziani RCS,Tavares DC,Bastos JK,Martins CHG

    更新日期:2018-02-21 00:00:00

  • Human Microbe-Disease Association Prediction With Graph Regularized Non-Negative Matrix Factorization.

    abstract::A microbe is a microscopic organism which may exists in its single-celled form or in a colony of cells. In recent years, accumulating researchers have been engaged in the field of uncovering microbe-disease associations since microbes are found to be closely related to the prevention, diagnosis, and treatment of many ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02560

    authors: He BS,Peng LH,Li Z

    更新日期:2018-11-01 00:00:00

  • Probiotic Enterococcus mundtii Isolate Protects the Model Insect Tribolium castaneum against Bacillus thuringiensis.

    abstract::Enterococcus mundtii strains isolated from the larval feces of the Mediterranean flour moth Ephestia kuehniella show antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria. The in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01261

    authors: Grau T,Vilcinskas A,Joop G

    更新日期:2017-07-07 00:00:00

  • Diversity of D-Amino Acid Utilizing Bacteria From Kongsfjorden, Arctic and the Metabolic Pathways for Seven D-Amino Acids.

    abstract::D-amino acids (DAAs) are an important component of the refractory dissolved organic matter pool in the ocean. Microbes play a vital role in promoting the recycling of DAAs in the ocean. However, the diversity of marine DAA-utilizing bacteria and how they metabolize DAAs are seldom studied. Here, by enrichment culture ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02983

    authors: Yu Y,Yang J,Zheng LY,Sheng Q,Li CY,Wang M,Zhang XY,McMinn A,Zhang YZ,Song XY,Chen XL

    更新日期:2020-01-10 00:00:00

  • Estimating HIV-1 Genetic Diversity in Brazil Through Next-Generation Sequencing.

    abstract::Approximately 36.7 million people were living with the human immunodeficiency virus (HIV) at the end of 2016 according to UNAIDS, representing a global prevalence rate of 0.8%. In Brazil, an HIV prevalence of 0.24% has been estimated, which represents approximately 830,000 individuals living with the virus. As a touri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00749

    authors: Alves BM,Siqueira JD,Prellwitz IM,Botelho OM,Da Hora VP,Sanabani S,Recordon-Pinson P,Fleury H,Soares EA,Soares MA

    更新日期:2019-04-09 00:00:00

  • Seasonality and depth distribution of the abundance and activity of ammonia oxidizing microorganisms in marine coastal sediments (North Sea).

    abstract::Microbial processes such as nitrification and anaerobic ammonium oxidation (anammox) are important for nitrogen cycling in marine sediments. Seasonal variations of archaeal and bacterial ammonia oxidizers (AOA and AOB) and anammox bacteria, as well as the environmental factors affecting these groups, are not well stud...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00472

    authors: Lipsewers YA,Bale NJ,Hopmans EC,Schouten S,Sinninghe Damsté JS,Villanueva L

    更新日期:2014-09-05 00:00:00

  • Paradigms of Protist/Bacteria Symbioses Affecting Human Health: Acanthamoeba species and Trichomonas vaginalis.

    abstract::Ever since the publication of the seminal paper by Lynn Margulis in 1967 proposing the theory of the endosymbiotic origin of organelles, the study of the symbiotic relationships between unicellular eukaryotes and prokaryotes has received ever-growing attention by microbiologists and evolutionists alike. While the evol...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2020.616213

    authors: Henriquez FL,Mooney R,Bandel T,Giammarini E,Zeroual M,Fiori PL,Margarita V,Rappelli P,Dessì D

    更新日期:2021-01-07 00:00:00

  • Different Transcriptional Responses from Slow and Fast Growth Rate Strains of Listeria monocytogenes Adapted to Low Temperature.

    abstract::Listeria monocytogenes has become one of the principal foodborne pathogens worldwide. The capacity of this bacterium to grow at low temperatures has opened an interesting field of study in terms of the identification and classification of new strains of L. monocytogenes with different growth capacities at low temperat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00229

    authors: Cordero N,Maza F,Navea-Perez H,Aravena A,Marquez-Fontt B,Navarrete P,Figueroa G,González M,Latorre M,Reyes-Jara A

    更新日期:2016-03-01 00:00:00

  • Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR.

    abstract::Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathoge...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02454

    authors: Rothrock MJ Jr,Feye KM,Kim SA,Park SH,Locatelli A,Hiett KL,Gamble J,Sellers H,Ricke SC

    更新日期:2018-11-02 00:00:00

  • Sterilization of liquid foods by pulsed electric fields-an innovative ultra-high temperature process.

    abstract::The intention of this study was to investigate the inactivation of endospores by a combined thermal and pulsed electric field (PEF) treatment. Therefore, self-cultivated spores of Bacillus subtilis and commercial Geobacillus stearothermophilus spores with certified heat resistance were utilized. Spores of both strains...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00400

    authors: Reineke K,Schottroff F,Meneses N,Knorr D

    更新日期:2015-05-06 00:00:00

  • Silencing of Anopheles stephensi Heme Peroxidase HPX15 Activates Diverse Immune Pathways to Regulate the Growth of Midgut Bacteria.

    abstract::Anopheles mosquito midgut harbors a diverse group of endogenous bacteria that grow extensively after the blood feeding and help in food digestion and nutrition in many ways. Although, the growth of endogenous bacteria is regulated by various factors, however, the robust antibacterial immune reactions are generally sup...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01351

    authors: Kajla M,Choudhury TP,Kakani P,Gupta K,Dhawan R,Gupta L,Kumar S

    更新日期:2016-08-31 00:00:00

  • Occurrence of Hepatitis E Virus in Pigs and Pork Cuts and Organs at the Time of Slaughter, Spain, 2017.

    abstract::Zoonotic hepatitis E, mainly caused by hepatitis E virus (HEV) genotype (gt) 3, is a foodborne disease that has emerged in Europe in recent decades. The main animal reservoir for genotype 3 is domestic pigs. Pig liver and liver derivates are considered the major risk products, and studies focused on the presence of HE...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02990

    authors: García N,Hernández M,Gutierrez-Boada M,Valero A,Navarro A,Muñoz-Chimeno M,Fernández-Manzano A,Escobar FM,Martínez I,Bárcena C,González S,Avellón A,Eiros JM,Fongaro G,Domínguez L,Goyache J,Rodríguez-Lázaro D

    更新日期:2020-01-28 00:00:00

  • Molecular Characterization of Fecal Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli From Healthy Companion Animals and Cohabiting Humans in South Korea.

    abstract::This study aimed to describe the distribution and characterization of fecal extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from healthy companion animals and cohabiting humans. A total of 968 rectal swab samples from 340 participants, including healthy companion animals and cohabiti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00674

    authors: Hong JS,Song W,Park HM,Oh JY,Chae JC,Jeong S,Jeong SH

    更新日期:2020-04-15 00:00:00

  • The Naphthalene Catabolic Genes of Pseudomonas putida BS3701: Additional Regulatory Control.

    abstract::Pseudomonas microorganisms are used for bioremediation of soils contaminated with petroleum hydrocarbons. The overall remediation efficiency is largely dependent on the presence of macro- and micronutrients. Widely varying concentrations of available nitrogen and iron (Fe) in soils were shown to affect residual hydroc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01217

    authors: Pozdnyakova-Filatova I,Petrikov K,Vetrova A,Frolova A,Streletskii R,Zakharova M

    更新日期:2020-06-05 00:00:00

  • Combatting cyanobacteria with hydrogen peroxide: a laboratory study on the consequences for phytoplankton community and diversity.

    abstract::Experiments with different phytoplankton densities in lake samples showed that a high biomass increases the rate of hydrogen peroxide (HP) degradation and decreases the effectiveness of HP in the selective suppression of dominant cyanobacteria. However, selective application of HP requires usage of low doses only, acc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00714

    authors: Weenink EF,Luimstra VM,Schuurmans JM,Van Herk MJ,Visser PM,Matthijs HC

    更新日期:2015-07-22 00:00:00

  • Relationships between Cytokine Profiles and Signaling Pathways (IκB Kinase and p38 MAPK) in Parainfluenza Virus-Infected Lung Fibroblasts.

    abstract::Respiratory viruses such as parainfluenza virus (PIV) in individuals with certain genetic predispositions in early life are associated with the induction of wheezing, which can progress to the development of asthma. It has been suggested that aberrant production of various cytokines due to viral infection are associat...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2010.00124

    authors: Yoshizumi M,Kimura H,Okayama Y,Nishina A,Noda M,Tsukagoshi H,Kozawa K,Kurabayashi M

    更新日期:2010-11-15 00:00:00

  • Corrigendum: Impact of Salicylic Acid and PGPR on the Drought Tolerance and Phytoremediation Potential of Helianthus annus.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2018.02507.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2019.02222

    authors: Khan N,Zandi P,Ali S,Mehmood A,Adnan Shahid M,Yang J

    更新日期:2019-09-27 00:00:00

  • In situ Immune Signatures and Microbial Load at the Nasopharyngeal Interface in Children With Acute Respiratory Infection.

    abstract::Acute respiratory infection (ARI) is the most frequent cause for hospitalization in infants and young children. Using multiplexed nCounter technology to digitally quantify 600 human mRNAs in parallel with 14 virus- and 5 bacterium-specific RNAs, we characterized viral and bacterial presence in nasopharyngeal aspirates...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02475

    authors: Fukutani KF,Nascimento-Carvalho CM,Bouzas ML,Oliveira JR,Barral A,Dierckx T,Khouri R,Nakaya HI,Andrade BB,Van Weyenbergh J,de Oliveira CI

    更新日期:2018-11-09 00:00:00

  • Microcalorimetric study of the growth of Streptococcus thermophilus in renneted milk.

    abstract::The growth of Streptococcus thermophilus ST12 (ST12) in liquid milk, reconstituted from low-heat skim milk powder reconstituted skim milk (RSM) and in RSM with rennet addition (r-RSM) at 40°C was monitored by microcalorimetry. It was shown that the growth rate of bacteria decreased in renneted samples in comparison wi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00079

    authors: Stulova I,Kabanova N,Kriščiunaite T,Adamberg K,Laht TM,Vilu R

    更新日期:2015-02-10 00:00:00

  • Assessment of Ruminal Bacterial and Archaeal Community Structure in Yak (Bos grunniens).

    abstract::The aim of this study was to determine the microbial community composition in the rumen of yaks under different feeding regimes. Microbial communities were assessed by sequencing bacterial and archaeal 16S ribosomal RNA gene fragments obtained from yaks (Bos grunniens) from Qinghai-Tibetan Plateau, China. Samples were...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00179

    authors: Zhou Z,Fang L,Meng Q,Li S,Chai S,Liu S,Schonewille JT

    更新日期:2017-02-07 00:00:00

  • Stimulation of the Internal Ribosome Entry Site (IRES)-Dependent Translation of Enterovirus 71 by DDX3X RNA Helicase and Viral 2A and 3C Proteases.

    abstract::The translation of enterovirus 71 (EV71) is mediated by an internal ribosome entry site (IRES)-dependent manner. EV71 IRES comprises five highly structured domains (domains II-VI) in the 5'-untranslated region of the viral mRNA. A conserved AUG triplet residing in domain VI is proposed to be the ribosome entry site. I...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01324

    authors: Su YS,Tsai AH,Ho YF,Huang SY,Liu YC,Hwang LH

    更新日期:2018-06-19 00:00:00

  • Bacterial-Fungal Interactions in the Kelp Endomicrobiota Drive Autoinducer-2 Quorum Sensing.

    abstract::Brown macroalgae are an essential component of temperate coastal ecosystems and a growing economic sector. They harbor diverse microbial communities that regulate algal development and health. This algal holobiont is dynamic and achieves equilibrium via a complex network of microbial and host interactions. We now repo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01693

    authors: Tourneroche A,Lami R,Hubas C,Blanchet E,Vallet M,Escoubeyrou K,Paris A,Prado S

    更新日期:2019-07-31 00:00:00

  • Biodegradation of Deoxynivalenol by a Novel Microbial Consortium.

    abstract::Deoxynivalenol (DON), a common mycotoxin of type B trichothecene, is produced mainly by several Fusarium species. DON causes great losses in farming and poses severe safety risks to human and animal health. Thus, DON contamination in cereals and DON toxicity are of worldwide concern. In this study, we screened the bac...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02964

    authors: Wang Y,Wang G,Dai Y,Wang Y,Lee YW,Shi J,Xu J

    更新日期:2020-01-08 00:00:00

  • The CagRS Two-Component System Regulates Clavulanic Acid Metabolism via Multiple Pathways in Streptomyces clavuligerus F613-1.

    abstract::Streptomyces clavuligerus F613-1 produces a clinically important β-lactamase inhibitor, clavulanic acid (CA). Although the biosynthesis pathway of CA has essentially been elucidated, the global regulatory mechanisms of CA biosynthesis remain unclear. The paired genes cagS and cagR, which are annotated, respectively, a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00244

    authors: Fu J,Qin R,Zong G,Liu C,Kang N,Zhong C,Cao G

    更新日期:2019-02-14 00:00:00