Urinary free cortisol assessment by liquid chromatography tandem mass spectrometry: a case study of ion suppression due to unacquainted administration of piperacillin.

Abstract:

Introduction:Liquid chromatography coupled to atmospheric pressure ionization tandem mass spectrometry (LC-ESI-MS/MS) is currently considered the reference method for quantitative determination of urinary free cortisol (UFC). One of the major drawbacks of this measurement is a particular form of matrix effect, conventionally known as ion suppression. Materials and methods:We describe here the case of a 66-year-old-patient referred to the daily service of general medicine for intravenous antibiotic administration due to a generalized Staphylococcus aureus infection and for routine 24 hours UFC monitoring in the setting of glucocorticoid replacement therapy. Results:The observation of 10-fold decrease of internal standard of cortisol signal led us to hypothesize the presence of an ion suppression effect due to a co-eluting endogenous compound. Screening analysis of tandem mass spectrometry (MS/MS) spectra of the interfering molecule, along with in vitro confirmation analyses, were suggestive of the presence of high concentration of piperacillin. The problem was then easily solved with minor modifications of the chromatographic technique. Conclusions:According to our findings, antibiotic therapy with piperacillin/tazobactam should be regarded as an important interference in UFC assessment, which may potentially affect detection capability, precision and accuracy of this measurement. This case report emphasizes that accurate anamnesis and standardization of all phases of urine collection are essential aspects for preventing potential interference in laboratory testing.

journal_name

Biochem Med (Zagreb)

journal_title

Biochemia medica

authors

Danese E,Salvagno GL,Guzzo A,Scurati S,Fava C,Lippi G

doi

10.11613/BM.2017.031001

subject

Has Abstract

pub_date

2017-10-15 00:00:00

pages

031001

issue

3

eissn

1330-0962

issn

1846-7482

pii

bm-27-3-031001

journal_volume

27

pub_type

杂志文章,评审
  • Molecular pathways in cancer-related inflammation.

    abstract::Accumulating evidence shows that chronic inflammation is associated to increased risk of cancer. An inflammatory component is present also in the microenvironment of tumours epidemiologically unrelated to inflammation. Extensive investigations over the past decade have uncovered many of the important mechanistic pathw...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/bm.2011.036

    authors: Del Prete A,Allavena P,Santoro G,Fumarulo R,Corsi MM,Mantovani A

    更新日期:2011-01-01 00:00:00

  • The logic of equivalence testing and its use in laboratory medicine.

    abstract::Hypothesis testing is a methodological paradigm widely popularized outside the field of pure statistics, and nowadays more or less familiar to the largest part of biomedical researchers. Conversely, the equivalence testing is still somehow obscure and misunderstood, although it represents a conceptual mainstay for som...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2017.001

    authors: Ialongo C

    更新日期:2017-02-15 00:00:00

  • IMPACT Observatory: tracking the evolution of clinical trial data sharing and research integrity.

    abstract:INTRODUCTION:The opening of research data is emerging thanks to the increasing possibilities of digital technology. The opening of clinical trial (CT) data is a part of this process, expected to have positive scientific, ethical, health, and economic impacts thus contributing to research integrity. The January 2016 pro...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2016.035

    authors: Krleža-Jerić K,Gabelica M,Banzi R,Martinić MK,Pulido B,Mahmić-Kaknjo M,Reveiz L,Šimić J,Utrobičić A,Hrgović I

    更新日期:2016-10-15 00:00:00

  • Analytical robustness of nine common assays: frequency of outliers and extreme differences identified by a large number of duplicate measurements.

    abstract:INTRODUCTION:Duplicate measurements can be used to describe the performance and analytical robustness of assays and to identify outliers. We performed about 235,000 duplicate measurements of nine routinely measured quantities and evaluated the observed differences between the replicates to develop new markers for analy...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2017.021

    authors: Neubig S,Grotevendt A,Kallner A,Nauck M,Petersmann A

    更新日期:2017-02-15 00:00:00

  • Economic evaluation of different screening alternatives for patients with clinically suspected acute deep vein thrombosis.

    abstract:INTRODUCTION:We examined the cost-effectiveness of the three different D-dimer measurements in the screening of DVT in models with and without calculation of pre-test probability (PTP) score. Moreover, we calculated the minimal cost in DVT detection. MATERIAL AND METHODS:In the group of 192 patients with clinically su...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/bm.2013.012

    authors: Bogavac-Stanojević N,Dopsaj V,Jelić-Ivanović Z,Lakić D,Vasić D,Petrova G

    更新日期:2013-01-01 00:00:00

  • Preanalytical phase--a continuous challenge for laboratory professionals.

    abstract::Preanalytical phase is the most vulnerable part of the total testing process and is considered to be among the greatest challenges to the laboratory professionals. However, preanalytical activities, management of unsuitable specimens and reporting policies are not fully standardized, nor harmonized worldwide. Several ...

    journal_title:Biochemia medica

    pub_type: 社论

    doi:10.11613/bm.2012.017

    authors: Simundic AM,Lippi G

    更新日期:2012-01-01 00:00:00

  • Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: 
a systematic review.

    abstract::This systematic review presents the latest trends in salivary research and its applications in health and disease. Among the large number of analytes present in saliva, many are affected by diverse physiological and pathological conditions. Further, the non-invasive, easy and cost-effective collection methods prompt a...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2015.018

    authors: Nunes LA,Mussavira S,Bindhu OS

    更新日期:2015-06-05 00:00:00

  • The diagnostic accuracy of clinical and laboratory parameters in the diagnosis of acute appendicitis in the adult emergency department population - a case control pilot study.

    abstract:Introduction:The evaluation of patients with suspected appendicitis strives to identify all patients with presenting symptoms while minimizing negative appendectomy rate. The aim of the study was to identify the optimal combination of clinical and laboratory parameters that should facilitate the emergency department su...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2018.030712

    authors: Soldo I,Radisic Biljak V,Bakula B,Bakula M,Simundic AM

    更新日期:2018-10-15 00:00:00

  • Scientific author names: errors, corrections, and identity profiles.

    abstract::Authorship problems are deep-rooted in the field of science communication. Some of these relate to lack of specific journal instructions. For decades, experts in journal editing and publishing have been exploring the authorship criteria and contributions deserving either co-authorship or acknowledgment. The issue of i...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2016.017

    authors: Gasparyan AY,Yessirkepov M,Gerasimov AN,Kostyukova EI,Kitas GD

    更新日期:2016-01-01 00:00:00

  • How to conduct External Quality Assessment Schemes for the pre-analytical phase?

    abstract::In laboratory medicine, several studies have described the most frequent errors in the different phases of the total testing process, and a large proportion of these errors occur in the pre-analytical phase. Schemes for registration of errors and subsequent feedback to the participants have been conducted for decades ...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2014.013

    authors: Kristensen GB,Aakre KM,Kristoffersen AH,Sandberg S

    更新日期:2014-02-15 00:00:00

  • Inflammatory and angiogenic biomarkers in diabetic retinopathy.

    abstract::Diabetic retinopathy (DR) is one of the most common microvascular complications of diabetes mellitus (DM) and a leading cause of blindness in working-age adults in developed countries. Numerous investigations have recognised inflammation and angiogenesis as important factors in the development of this complication of ...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2020.030502

    authors: Kaštelan S,Orešković I,Bišćan F,Kaštelan H,Gverović Antunica A

    更新日期:2020-10-15 00:00:00

  • The chi-square test of independence.

    abstract::The Chi-square statistic is a non-parametric (distribution free) tool designed to analyze group differences when the dependent variable is measured at a nominal level. Like all non-parametric statistics, the Chi-square is robust with respect to the distribution of the data. Specifically, it does not require equality o...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/bm.2013.018

    authors: McHugh ML

    更新日期:2013-01-01 00:00:00

  • Determining biological variation of serum parathyroid hormone in healthy adults.

    abstract:Introduction:Measurement of parathyroid hormone (PTH) is essential in the investigation and management of calcium metabolism disorders. To assess the significance of any assay result when clinical decision making biological variation (BV) of the measurand must be taken into consideration. The aim of the present study i...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2019.030702

    authors: Ercan M,Akbulut ED,Avcı E,Yücel Ç,Oğuz EF,Turhan T,Serdar M

    更新日期:2019-10-15 00:00:00

  • External Quality Assessment in Croatia: problems, challenges, and specific circumstances.

    abstract::In Croatian medical laboratories (ML), external quality assessment (EQA) has a long tradition of almost half of a century. At national level, EQA is provided by Croatian Centre for Quality Assessment in Laboratory Medicine (CROQALM) which is a part of Croatian Society of Medical Biochemistry and Laboratory Medicine.
T...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2017.011

    authors: Krleza JL,Celap I,Tanaskovic JV

    更新日期:2017-02-15 00:00:00

  • Oxidative status parameters in children with urinary tract infection.

    abstract:INTRODUCTION:Urinary tract infection (UTI) is one of the most common bacterial infectious diseases in children. The aim of this study was to determine the total prooxidant and antioxidant capacity of children with UTI, as well as changes of oxidative status parameters according to acute inflammation persistence and acu...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2014.029

    authors: Petrovic S,Bogavac-Stanojevic N,Kotur-Stevuljevic J,Peco-Antic A,Ivanisevic I,Ivanisevic J,Paripovic D,Jelic-Ivanovic Z

    更新日期:2014-01-01 00:00:00

  • Effect of winter swimming on haematological parameters.

    abstract:INTRODUCTION:Winter swimming represents an intensive short-term exposure to cold, and thus it is considered a strong physical stress. Cold-based treatments, i.e. immersions in cold water, are spreading in sport medicine for improving recovery following muscle traumas, although a universal acceptance of that method is n...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/bm.2011.014

    authors: Lombardi G,Ricci C,Banfi G

    更新日期:2011-01-01 00:00:00

  • A review of urinary angiotensin converting enzyme 2 in diabetes and diabetic nephropathy.

    abstract::Urinary angiotensin converting enzyme 2 (ACE2) is significantly increased in diabetes and diabetic nephropathy. While studies on its clinical significance are still underway, its urinary expression, association with metabolic and renal parameters has been in the recent past considerably studied. The recent studies hav...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2019.010501

    authors: Gilbert A,Liu J,Cheng G,An C,Deo K,Gorret AM,Qin X

    更新日期:2019-02-15 00:00:00

  • A paradigmatic case of haemolysis and pseudohyperkalemia in blood gas analysis.

    abstract::A 51-year old male patient was admitted to the hospital with acute dyspnea and history of chronic asthma. Venous blood was drawn into a 3.0 mL heparinized syringe and delivered to the laboratory for blood gas analysis (GEM Premier 4000, Instrumentation Laboratory), which revealed high potassium value (5.2 mmol/L; refe...

    journal_title:Biochemia medica

    pub_type:

    doi:10.11613/BM.2019.011003

    authors: Salvagno GL,Demonte D,Lippi G

    更新日期:2019-02-15 00:00:00

  • In vitro diagnostic company recalls and medical laboratory practices: an Italian case.

    abstract:INTRODUCTION:In vitro human diagnostic (IVD) company recalls are a common practice aimed to either minimize a potential error or eliminate an existing failure. In this case report, we aim to provide a critical analysis of a recent IVD recall and to provide a practical framework about what to do when an IVD company reca...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2015.028

    authors: Lima-Oliveira G,Lippi G,Salvagno GL,Brocco G,Guidi GC

    更新日期:2015-06-05 00:00:00

  • Laboratory diagnostics of chronic kidney disease in Croatia: state of the art.

    abstract:INTRODUCTION:Early identification and management of chronic kidney disease (CKD) is highly cost-effective and can reduce the risk of kidney failure progression and cardiovascular disease. In 2014, the Joint Croatian Working Group (JCWG) for laboratory diagnostic of CKD on the behalf of Croatian society of medical bioch...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2015.009

    authors: Biljak VR,Honović L,Matica J,Knežević B,Vojak SŠ

    更新日期:2015-01-01 00:00:00

  • Detection of acute kidney injury in premature asphyxiated neonates by serum neutrophil gelatinase-associated lipocalin (sNGAL)--sensitivity and specificity of a potential new biomarker.

    abstract:INTRODUCTION:Acute kidney injury (AKI) is common in neonatal intensive care units (NICU). In recent years, every effort is made for early detection of AKI. Our hypothesis was that serum neutrophil gelatinase-associated lipocalin (sNGAL) may be a reliable screening test for early diagnosis of AKI in premature neonates a...

    journal_title:Biochemia medica

    pub_type: 临床试验,杂志文章

    doi:10.11613/BM.2015.046

    authors: Pejović B,Erić-Marinković J,Pejović M,Kotur-Stevuljević J,Peco-Antić A

    更新日期:2015-10-15 00:00:00

  • Storage stability of commonly used haematological parameters at 33 °C: Electronic supplementary material available online for this article.

    abstract:Introduction:This study aimed to investigate the analytical bias in haematological parameters induced by storage at 33 ºC. Materials and methods:Blood from the diversion pouch of 20 blood donors were collected in K 2 EDTA vials and stored at 33 ºC. Readings from each vial ...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2018.020901

    authors: Jain A,Jain S,Singh N,Aswal P,Pal S,Meinia SK,Chowdhury N

    更新日期:2018-06-15 00:00:00

  • The most common nonconformities encountered during the assessments of medical laboratories in Hong Kong using ISO 15189 as accreditation criteria.

    abstract:INTRODUCTION:ISO 15189 was a new standard published in 2003 for accrediting medical laboratories. We believe that some requirements of the ISO 15189 standard are especially difficult to meet for majority of laboratories. The aim of this article was to present the frequency of nonconformities to requirements of the ISO ...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/bm.2012.027

    authors: Ho B,Ho E

    更新日期:2012-01-01 00:00:00

  • Optimizing laboratory defined macroprolactin algorithm.

    abstract:Introduction:Macroprolactinaemia is a well-known analytical problem in diagnostics of hyperprolactinaemia usually detected with polyethylene glycol (PEG) precipitation method. Since there is no harmonization in macroprolactin detection and reporting results, this study proposes and evaluates the usefulness of in-house ...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2019.020706

    authors: Šostarić M,Bokulić A,Marijančević D,Zec I

    更新日期:2019-06-15 00:00:00

  • Additional technician tasks and turnaround time in the clinical Stat laboratory.

    abstract:INTRODUCTION:Many additional tasks in the Stat laboratory (SL) increase the workload. It is necessary to control them because they can affect the service provided by the laboratory. Our aim is to calculate these tasks, study their evolution over a 10 year period, and compare turnaround times (TAT) in summer period to t...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2016.027

    authors: Salinas M,López-Garrigós M,Flores E,Leiva-Salinas M,Lillo R,Leiva-Salinas C

    更新日期:2016-01-01 00:00:00

  • ICMJE authorship criteria are not met in a substantial proportion of manuscripts submitted to Biochemia Medica.

    abstract:INTRODUCTION:Our aim was to investigate if: (a) authors of Biochemia Medica meet authorship criteria given by International Committee of Medical Journal Editors (ICMJE), (b) authorship violations are more frequent in submissions containing some type of scientific misconduct. MATERIALS AND METHODS:Self-reported authors...

    journal_title:Biochemia medica

    pub_type: 杂志文章,评审

    doi:10.11613/BM.2015.033

    authors: Šupak-Smolčić V,Mlinarić A,Antončić D,Horvat M,Omazić J,Šimundić AM

    更新日期:2015-10-15 00:00:00

  • Evaluating analytical quality in clinical biochemistry laboratory using Six Sigma.

    abstract:Introduction:In recent years, Six Sigma metrics has became the hotspot in all trades and professions, which contributes a general procedure to explain the performance on sigma scale. Nowadays, many large companies, such as General Healthcare, Siemens, etc., have applied Six Sigma to clinical medicine and achieved satis...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2018.020904

    authors: Mao X,Shao J,Zhang B,Wang Y

    更新日期:2018-06-15 00:00:00

  • Understanding logistic regression analysis.

    abstract::Logistic regression is used to obtain odds ratio in the presence of more than one explanatory variable. The procedure is quite similar to multiple linear regression, with the exception that the response variable is binomial. The result is the impact of each variable on the odds ratio of the observed event of interest....

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2014.003

    authors: Sperandei S

    更新日期:2014-02-15 00:00:00

  • Topiramate induced metabolic acidosis and kidney stones - a case study.

    abstract:INTRODUCTION:The aim of this study is to present a case of 44 years old woman with topiramate induced metabolic acidosis and kidney stones. MATERIALS AND METHODS:The laboratory features of topiramate caused renal tubular acidosis in blood and urine during topiramate treatment, with correction of metabolic acidosis by ...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2017.042

    authors: Salek T,Andel I,Kurfurstova I

    更新日期:2017-06-15 00:00:00

  • Peer-review policy and guidelines for Biochemia Medica Journal.

    abstract::Peer review is widely used system for evaluating manuscripts prior to publication. It has been and still is widely used tool for making justified and fair editorial decision. However, the evidence of its efficacy is limited and it has been criticized to be time-consuming, biased, inconsistent, conservative, and open t...

    journal_title:Biochemia medica

    pub_type: 杂志文章

    doi:10.11613/BM.2014.034

    authors: Smolčić VŠ,Simundić AM

    更新日期:2014-10-15 00:00:00