Abstract:
:In our previous studies, heme was bound into honeybee silk to generate materials that could function as nitric oxide sensors or as recoverable heterogeneous biocatalysts. In this study, we sought to increase the heme-binding capacity of the silk protein by firstly redesigning the heme binding site to contain histidine as the coordinating residue and secondly, by adding multiple histidine residues within the core of the coiled coil core region of the modified silk protein. We used detergent and a protein denaturant to confirm the importance of the helical structure of the silk for heme coordination. Aqueous methanol treatment, which was used to stabilize the materials, transformed the low-spin, six-coordinate heme to a five-coordinate high-spin complex, thus providing a vacant site for ligand binding. The optimal aqueous methanol treatment time that simultaneously maintains the helical protein structure and stabilizes the silk material without substantial leaching of heme from the system was determined.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Rapson TD,Liu JW,Sriskantha A,Musameh M,Dunn CJ,Church JS,Woodhead A,Warden AC,Riley MJ,Harmer JR,Noble CJ,Sutherland TDdoi
10.1016/j.jinorgbio.2017.08.031subject
Has Abstractpub_date
2017-12-01 00:00:00pages
219-227eissn
0162-0134issn
1873-3344pii
S0162-0134(17)30290-8journal_volume
177pub_type
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/s0162-0134(01)00184-2
更新日期:2001-04-01 00:00:00
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2018.11.015
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2016.10.003
更新日期:2016-12-01 00:00:00
abstract::The synthesis and characterization of ruthenium compounds of the type [RuCl(2)(NO)(dppp)(L)]PF(6) [dppp=1,3-bis(diphenylphosphino)propane; L=pyridine, 4-methylpyridine, 4-phenylpyridine and dimethyl sulfoxide] are described. The complexes were characterized by elemental analysis, UV/Vis and infrared spectroscopy, cycl...
journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2009.12.015
更新日期:2010-05-01 00:00:00
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2016.12.005
更新日期:2017-03-01 00:00:00
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journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/j.jinorgbio.2010.11.010
更新日期:2011-02-01 00:00:00
abstract::Electron paramagnetic resonance (e.p.r.) studies of thin DNA-chelate films indicate that both the water soluble planar macrocyclic chelates, cobalt (II) tetra (2,3-N-methylpyridinium) porphyrazine (cobalt (II) TMTPPA), and vanadyl (II) 5,10,15,20-tetra-(4-methylpyridinium) porphyrin (Vo (II) TMTPyP) can bind to calf t...
journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/0162-0134(88)85021-9
更新日期:1988-10-01 00:00:00
abstract::Iron deficiency anemia is a relatively common illness that can arise from a number of different causes. Three ferrous salts are usually used in its treatment: ferrous fumarate, gluconate, and sulfate. They are administered orally and are relatively well tolerated. These hematinics have been studied by Mössbauer spectr...
journal_title:Journal of inorganic biochemistry
pub_type: 杂志文章
doi:10.1016/0162-0134(94)00062-f
更新日期:1995-06-01 00:00:00