Abstract:
:The purpose of the study was to examine the effects of arterial coculture conditions on the transport properties of several in vitro endothelial cell (EC)-smooth muscle cell (SMC)-porous filter constructs in which SMC were grown to confluence first and then EC were inoculated. This order of culturing simulates the environment of a blood vessel wall after endothelial layer damage due to stenting, vascular grafting or other vascular wall insult. For all coculture configurations examined, we observed that hydraulic conductivity (L(p)) values were significantly higher than predicted by a resistances-in-series (RIS) model accounting for the L(p) of EC and SMC measured separately. The greatest increases were observed when EC were plated directly on top of a confluent SMC layer without an intervening filter, presumably mediated by direct EC-SMC contacts that were observed under confocal microscopy. The results are the opposite of a previous study that showed L(p) was significantly reduced compared to an RIS model when EC were grown to confluency first. The physiological, pathophysiological and tissue engineering implications of these results are discussed.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Mathura RA,Russell-Puleri S,Cancel LM,Tarbell JMdoi
10.1007/s10439-015-1421-5subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1721-33issue
5eissn
0090-6964issn
1573-9686pii
10.1007/s10439-015-1421-5journal_volume
44pub_type
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