Abstract:
:The purpose of this study is to characterize synovial fluid- (SF-) derived exosomes of patients with gonarthrosis comparing two methods of isolation and to investigate their immune regulatory properties. Extracellular vesicles (EVs) have been isolated from inflamed SF by polymer precipitation method and quantified by Exocet kit and by nanoparticle tracking analysis. Vesicles expressed all the specific exosomal markers by immunoblot and FACS. After isolation with Exoquick, a relevant contamination by immune complexes was detected, which required further magnetic bead-based purification to remove. SF-derived exosomes significantly stimulated the release of several inflammatory cytokines and chemokines and metalloproteinases by M1 macrophages but did not influence the expression of CD80 and CD86 costimulatory molecules. In conclusion, we characterized purified exosomes isolated from inflamed SF and demonstrate that purified exosomes are functionally active in their ability to stimulate the release of proinflammatory factors from M1 macrophages. Our data indicate that SF-derived exosomes from gonarthrosis patients play a role in disease progression.
journal_name
Mediators Inflammjournal_title
Mediators of inflammationauthors
Domenis R,Zanutel R,Caponnetto F,Toffoletto B,Cifù A,Pistis C,Di Benedetto P,Causero A,Pozzi M,Bassini F,Fabris M,Niazi KR,Soon-Shiong P,Curcio Fdoi
10.1155/2017/4814987subject
Has Abstractpub_date
2017-01-01 00:00:00pages
4814987eissn
0962-9351issn
1466-1861journal_volume
2017pub_type
杂志文章abstract::Multiple sclerosis is an autoimmune disease with classical traits of demyelination, axonal damage, and neurodegeneration. The migration of autoimmune T cells and macrophages from blood to central nervous system as well as the destruction of blood brain barrier are thought to be the major processes in the development o...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2014/659206
更新日期:2014-01-01 00:00:00
abstract::CXCL8 displays several tumor-promoting effects. Targeting and/or lowering CXCL8 concentrations within the tumor microenvironment would produce a therapeutic benefit. Aim of this study was to test the effect of IFNγ on the basal and TNFα-stimulated secretion of CXCL8 in TCP-1 and BCPAP thyroid cancer cell lines (harbor...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/8512417
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abstract::An underlying endothelial dysfunction plays a fundamental role in the pathogenesis of cardiovascular events and is the central feature of atherosclerosis. The protein-based communication between leukocytes and inflamed endothelial cells leading to diapedesis has been largely investigated and several key players such a...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2015/737310
更新日期:2015-01-01 00:00:00
abstract::Since iron can contribute to detrimental radical generating processes through the Fenton and Haber-Weiss reactions, it seems to be a reasonable approach to modulate iron-related pathways in inflammation. In the human organism a counterregulatory reduction in iron availability is observed during inflammatory diseases. ...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2015/516740
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abstract::Thymosin beta(4) is a polypeptide isolated from thymosin fraction 5. This peptide exhibits important activities in the regulation and differentiation of thymus-dependent lymphocytes. An analogue of thymosin beta(4), [Phe(4F)(12)] deacetyl- thymosin beta(4), was synthesized by a solution method, followed by deprotectio...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S096293519200019X
更新日期:1992-01-01 00:00:00
abstract::Cardiovascular (CV) disease is the most common cause of premature mortality in patients with rheumatoid arthritis (RA). It is the result of an accelerated atherosclerotic process. Both RA and atherosclerosis are complex polygenic diseases. Besides traditional CV risk factors and chronic inflammation, a number of studi...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2012/574817
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abstract::The causes of retinal hypoxia are many and varied. Under hypoxic conditions, a variety of soluble factors are secreted into the vitreous cavity including growth factors, cytokines, and chemokines. Cytokines, which usually serve as signals between neighboring cells, are involved in essentially every important biologica...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/935301
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abstract::Obesity characterized by increased mass of adipose tissue leads to systemic inflammation. Calorie restriction (CR) improves parameters associated with immune response and antioxidant defense. We hypothesized that CR with a high fat diet (HFCR) regulates local and systemic inflammation and oxidative stress damage in a ...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2012/984643
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Influenza virus is a worldwide health problem with significant economic consequences. To study the gene expression pattern induced by influenza virus infection, it is useful to reveal the pathogenesis of influenza virus infection; but this has not been well examined, especially in vivo study. AIMS:To assess...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/0962935021000051557
更新日期:2002-12-01 00:00:00
abstract::We previously demonstrated that Bothrops jararaca venom (BjV) has an antitumor effect on Ehrlich ascites tumor (EAT) cells and induces an increase of polymorphonuclear leukocytes in early stages of tumor growth. It has been reported that this venom presents an important inflammatory effect when inoculated in animal mo...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/0962935029000041
更新日期:2002-08-01 00:00:00
abstract::Stimulation of phosphoinositide hydrolysis in myocardium from autoimmune myocarditis mice by ThEA and histamine was assayed. Myocardium from autoimmune heart, but not the normal forms, specifically increased phosphoinositide turnover in the presence of histaminergic agonists. This increment was blocked by a specific H...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935193000444
更新日期:1993-01-01 00:00:00
abstract:Objective:Renal ischemia/reperfusion injury (RI/RI) is the main cause of acute kidney injury. Total glucosides of paeony (TGP) are a traditional Chinese medicine. This study was aimed at exploring the role of TGP in RI/RI and its underlying mechanism of action. Methods:Rat RI/RI models were constructed by surgical ope...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2020/8869511
更新日期:2020-11-24 00:00:00
abstract::Human respiratory syncytial virus (RSV) sometimes causes acute and severe lower respiratory tract illness in infants and young children. RSV strongly upregulates proinflammatory cytokines and the platelet-activating factor (PAF) receptor, which is a receptor for Streptococcus pneumoniae, in the pulmonary epithelial ce...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2012/528568
更新日期:2012-01-01 00:00:00
abstract::Systemic sclerosis (SSc) is an autoimmune connective tissue disorder of unknown etiology. This disease is characterized by a large variety of clinical patterns, which include the fibrosis of skin and visceral organs causing a variety of clinical manifestations. Genetic and environmental factors participate in the etio...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2014/389582
更新日期:2014-01-01 00:00:00
abstract::Tuberculosis is characterized by the presence of activated mononuclear cells both in the peripheral circulation and in pleural fluid. Expression and up-regulation of adhesion molecules is the basis of cell-cell adhesion in granuloma formation and in leukocyte migration to the inflammatory site. Soluble isoforms of adh...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935196000403
更新日期:1996-01-01 00:00:00
abstract::This study was designated to evaluate the influence of behavioral depression on the airway leukocyte recruitment in allergic animals. To achieve this, total and differential cell counts in bronchoalveolar (BAL) fluid of ovalbumin (OVA)-sensitized and depressed rats was evaluated. Inescapable electric footshock, applie...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/09629350124118
更新日期:2001-08-01 00:00:00
abstract::Our understanding of inflammation's role in the pathogenesis of myeloproliferative neoplasm (MPN) is evolving. The impact of chronic inflammation, a characteristic feature of MPN, likely goes far beyond its role as a driver of constitutional symptoms. An inflammatory response to the neoplastic clone may be responsible...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2015/606819
更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:In the present study, the ability of magnolol, a hydroxylated biphenyl compound isolated from Magnolia officinalis, to stimulate osteoblast function and inhibit the release of bone-resorbing mediators was investigated in osteoblastic MC3T3-E1 cells. METHODS:Osteoblast function was measured by cell growth, a...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2012/829650
更新日期:2012-01-01 00:00:00
abstract::Early onset sepsis due to group B streptococcus leads to neonatal morbidity, increased mortality, and long-term neurological deficencies. Interaction between septicemic GBS and confluent monolayers of human coronary artery endothelial cells (HCAECs) was analyzed by genome wide expression profiling. In total, 124 genes...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2011/971502
更新日期:2011-01-01 00:00:00
abstract::The generation of ROS and lipid peroxidation has been considered to play an important role in the pathogenesis of chronic fluoride toxicity. In the present study, we observed that fluoride activated BV-2 microglia cell line by observing OX-42 expression in immunocytochemistry. Intracellular superoxide dismutase (SOD),...
journal_title:Mediators of inflammation
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doi:10.1155/2012/102954
更新日期:2012-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic inflammation of multiple joints. The central pathogenesis of RA is the proliferation of synovial fibroblasts in response to inflammatory cytokines. However, some of the targeted therapies for inflammation reactions do not display signi...
journal_title:Mediators of inflammation
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doi:10.1155/2015/460310
更新日期:2015-01-01 00:00:00
abstract::Osteoporosis is a civilization disease which is still challenging for contemporary medicine in terms of treatment and prophylaxis. It results from excessive activation of the osteoclastic cell line and immune cells like macrophages and lymphocytes. Cell-to-cell inflammatory information transfer occurs via factors incl...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2019/7570452
更新日期:2019-11-12 00:00:00
abstract::A TP II analogue, [1-Nal3] TP II, was synthesized by a conventional solution method, followed by deprotection with 1M TFMSA-thioanisole (molar ratio 1:1) in TFA in the presence of Me2Se and m-cresol as scavengers. The synthetic [1-Nal3] TP II, TP II and [Phe (4 F)3] TP II were tested for comparative effect on the impa...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/09629350020002930
更新日期:2000-01-01 00:00:00
abstract::Interleukin-5 (IL-5) is a cytokine that preferentially effects the development and function of eosinophils, and is considered important in the pathophysiology of allergic inflammation. In this study, we evaluated the ability of recombinant human IL-5 (rHu IL-5) to promote tissue eosinophilia and the importance of this...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935195000536
更新日期:1995-01-01 00:00:00
abstract::It is hypothesized that central nervous system inflammation induced by systematic inflammation due to surgical trauma plays a critical role in postoperative cognitive dysfunction. The potential inhibitory effect of nerve blockage with local anesthetics on peripheral inflammatory response has been reported. We hypothes...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2015/460125
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND/AIM:Coronary artery ectasia (CAE) was thought of as a variant of atherosclerosis. C-reactive protein (CRP) which is among the most sensitive markers of systemic inflammation, and elevation of systemic and local levels of this inflammatory marker which has been associated with an increased risk for cardiovasc...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2007/89649
更新日期:2007-01-01 00:00:00
abstract::As one of the ligands of aryl hydrocarbon receptor (AhR), baicalein, isolated from Scutellaria baicalensis Georgi, has been proved to exert potential therapeutic effects on ulcerative colitis (UC), but its therapeutic mechanism remains obscure. Authentically, ulcerative colitis can be alleviated by regulating the diff...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2020/5918587
更新日期:2020-10-05 00:00:00
abstract::Sanguisorbae Radix (SR) is well known as herbal medicine named "Zi-Yu" in Korea, which is the dried roots of Sanguisorba officinalis L. (Rosacease). We investigated the underlying mechanism on the inhibition of atopic dermatitis (AD) of an ethanol extract of SR (ESR) using 2,4-dinitrochlorobenzene- (DNCB-) induced AD ...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/2947390
更新日期:2016-01-01 00:00:00
abstract::Interleukin 35 (IL-35) is a heterodimeric cytokine involved in the development of atherosclerosis. The aim of the present study was to establish if the polymorphisms of IL-12A and EBI3 genes that encode the IL-35 subunits are associated with the development of premature coronary artery disease (CAD) in Mexican individ...
journal_title:Mediators of inflammation
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更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Asthma is characterized by airway remodeling. Follistatin-like protein 1 (FSTL1) is an extracellular glycoprotein. Recent studies suggest that FSTL1 may participate in the pathogenesis of asthma. OBJECTIVES:To analyze the association between FSTL1 and some parameters and inspect the role of FSTL1 in asthma....
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2017/7918472
更新日期:2017-01-01 00:00:00