Abstract:
:The aim of the present study was to investigate the effect of metformin on endothelial progenitor cell (EPC) migration and to explore the possible mechanisms. EPCs were treated with metformin, and the migration of EPCs was evaluated by wound healing and Matrigel invasion assays. We also examined the expression levels of of MMP-2 and MMP-9 in EPCs with or without metformin treatment via RT-PCR and western blot analysis, and activities of MMP-2 and MMP-9 in EPCs under different conditions was examined by zymography. Moreover, we also assessed the AMPK/mTOR/autophagy pathway to explore the possible mechanisms. Metformin treatment significantly downregulated matrix metalloproteinase-2 (MMP-2) and MMP-9 expression, and subsequently decreased the migration of EPCs. Increased levels of phosphorylated (p)-AMPK and LC3II expression, as well as decreased levels of p-mTOR and p62 contributed to this phenomenon. The AMPK inhibitor compound C reversed the effect exerted by metformin. In conclusion, our results showed that metformin inhibited the migration of EPCs by decreasing MMP-2 and MMP-9. The AMPK/mTOR/autophagy pathway was demonstrated to be involved in the regulatory mechanisms.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Li WD,Li NP,Song DD,Rong JJ,Qian AM,Li XQdoi
10.3892/ijmm.2017.2929subject
Has Abstractpub_date
2017-05-01 00:00:00pages
1262-1268issue
5eissn
1107-3756issn
1791-244Xjournal_volume
39pub_type
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journal_title:International journal of molecular medicine
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doi:10.3892/ijmm.2018.3664
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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journal_title:International journal of molecular medicine
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