Abstract：
:Atherosclerosis is now considered a chronic inflammatory disease, and glucosamine has the potential to exhibit an anti-inflammatory action. Thus, we investigated the effect of glucosamine on tumor necrosis factor alpha (TNF-alpha)-induced endothelial cell activation. Human umbilical vein endothelial cells (HUVECs) were stimulated by TNF-alpha in the presence or absence of glucosamine or its analogue, N-acetylglucosamine. mRNA expression of MCP-1 (a chemoattractant protein) and ICAM-1 (an adhesion molecule) was evaluated by real-time RT-PCR, and their protein levels were analyzed by ELISA and Western blotting, respectively. Furthermore, the effects of glucosamine on the phosphorylation of p38MAPK and NF-kappaB, and O-N-acetylglucosamine (O-GlcNAc) modification were evaluated by Western blotting. The results demonstrated that glucosamine but not N-acetylglucosamine suppressed TNF-alpha-induced expression of MCP-1 and ICAM-1 at both the mRNA and protein levels. Furthermore, glucosamine abrogated the phosphorylation of p38MAPK and NF-kappaB. To note, glucosamine induced O-GlcNAc modification, which was negatively correlated with the expression of MCP-1 and ICAM-1, and phosphorylation of p38MAPK and NF-kappaB. Thus, glucosamine is likely to suppress endothelial cell activation (TNF-alpha-induced ICAM-1 and MCP-1 expression) possibly by affecting p38MAPK and NF-kappaB signaling via O-GlcNAc modification.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Ju Y,Hua J,Sakamoto K,Ogawa H,Nagaoka Isubject
Has Abstractpub_date
2008-12-01 00:00:00pages
809-15issue
6eissn
1107-3756issn
1791-244Xjournal_volume
22pub_type
杂志文章abstract：:FGF and WNT signaling pathways network together during embryogenesis and carcinogenesis. Among 22 FGF family members within human and rodents genomes, FGF20 orthologs are evolutionarily conserved targets of the WNT/beta-catenin signaling pathway. FGF8, FGF17, and FGF18 constitute one of FGF subfamilies. Here, comparat...
journal_title：International journal of molecular medicine
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doi：
更新日期：2005-09-01 00:00:00
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journal_title：International journal of molecular medicine
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journal_title：International journal of molecular medicine
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journal_title：International journal of molecular medicine
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pub_type： 杂志文章,评审
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更新日期：2005-02-01 00:00:00
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journal_title：International journal of molecular medicine
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更新日期：2007-02-01 00:00:00
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journal_title：International journal of molecular medicine
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journal_title：International journal of molecular medicine
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更新日期：2001-09-01 00:00:00
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