Abstract:
:Secondary tumours in the brain account for 40 % of triple negative breast cancer patients, and the percentage may be higher at the time of autopsy. The use of in vivo models allow us to recapitulate the molecular mechanisms potentially used by circulating breast tumour cells to proliferate within the brain.Metastasis is a multistep process that depends on the success of several stages including cell evasion from the primary tumour, distribution and survival within the blood stream and cerebral microvasculature, penetration of the blood-brain barrier and proliferation within the brain microenvironment. Cellular adhesion molecules are key proteins involved in all of the steps in the metastatic process. Our group has developed two different in vivo models to encompass both seeding and colonisation stages of the metastatic process: (1) haematogenous dissemination of tumour cells by direct injection into the left ventricle of the heart, and (2) direct implantation of the tumour cells into the mouse brain.This chapter describes, in detail, the practical implementation of the intracerebral model, which can be used to analyse tumour proliferation within a specific area of the central nervous system and tumour-host cell interactions. We also describe the use of immunohistochemistry techniques to identify, at the molecular scale, tumour-host cell interactions, which may open new windows for brain metastasis therapy.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Soto MS,Sibson NRdoi
10.1007/978-3-319-26666-4_13subject
Has Abstractpub_date
2016-01-01 00:00:00pages
231-44eissn
0065-2598issn
2214-8019journal_volume
899pub_type
杂志文章abstract::A high frequency of recombination has been shown to occur during replication of the coronavirus mouse hepatitis virus (MHV) in vitro as well as in vivo. Although sequencing of field strains of coronavirus infectious bronchitis virus (IBV) has indicated that IBV strains also undergo recombination, there has been no exp...
journal_title:Advances in experimental medicine and biology
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abstract::Before a messenger RNA (mRNA) is translated into a protein in the cytoplasm, its pre-mRNA precursor is extensively processed through capping, splicing and polyadenylation in the nucleus. Defects in the processing of pre-mRNAs due to mutations in RNA sequences often cause disease. Traditional small molecules or protein...
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abstract::The virus-derived protein Apoptin has the ability to induce p53-independent apoptosis in a variety of human cancer cells while leaving normal cells unharmed. It thus represents a potential anti-cancer therapeutic agent of the future but a proper understanding of Apoptin-induced signalling events is necessary prior to ...
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journal_title:Advances in experimental medicine and biology
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journal_title:Advances in experimental medicine and biology
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章
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pub_type: 杂志文章
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abstract::In the second half of the 20th century it became obvious that a relentless increase in Type 2 diabetes mellitus (T2DM), affecting the economically affluent countries, is gradually afflicting also the developing world. This chapter shows the threat that the T2DM epidemic represents to mankind, with the astonishing rece...
journal_title:Advances in experimental medicine and biology
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doi:10.1007/978-1-4614-5441-0_6
更新日期:2012-01-01 00:00:00
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abstract::Respiratory failure is one of the most important risk factors for diagnostic bronchofiberoscopy (BF), whereas therapeutic bronchoscopies are typically performed in intubated patients. Only a few published studies analyzed the outcomes of noninvasive mechanical ventilation (NIV)-facilitated BF. In this case series, we ...
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journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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abstract::The perception of the timing of milk arrival has been identified as an important predictor of breast-feeding success. Because this indicator has the potential to be used as a proxy for the onset of lactogenesis stage II and is relatively simple to measure, it is possible to include it as part of large-scale surveys. I...
journal_title:Advances in experimental medicine and biology
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abstract::Endometrial cancer is a frequently occurring gynecological disorder. Estrogen-dependent endometrioid carcinoma is the most common type of gynecological cancer. One of the major pathologic phenomena of endometrial cancer is the loss of estrogen (E2) and progesterone (P4) control over uterine epithelial cell proliferati...
journal_title:Advances in experimental medicine and biology
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abstract::This study surveyed that the relationship between the frequencies of intake of taurine-contained nutritional drinks (TCND), and lifestyle and the purposes of intake it. The study was conducted a cross-sectional survey using 265 people (203 male, 62 female) aged 18-64 worked in two companies in Mie Prefecture, Japan be...
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abstract::Erythropoietin producing cells were identified in the murine hypoxic kidney by in situ hybridization. The positive cells were peritubular cells, most likely endothelial cells of the cortex and outer medulla. Glomerular and tubular cells were not labelled. In three patients with renal adenocarcinomas associated with po...
journal_title:Advances in experimental medicine and biology
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abstract::A single compartment model of the arterial circulation was used to generate an arterial blood pressure waveform from pre-determined stroke volume (SV) and arterial resistance (R). With fixed stroke volume and varying resistances blood pressure waveforms showed mean values proportional to resistance but amplitude lesse...
journal_title:Advances in experimental medicine and biology
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abstract::This chapter gives an overview of present knowledge and clinical aspects of antidiabetic drugs according to the recently available research evidence and clinical expertise.Many agents are acting on eight groups of pathophysiological mechanisms, which is commonly called as "Ominous Octet" by DeFronzo. The muscle, liver...
journal_title:Advances in experimental medicine and biology
pub_type: 杂志文章,评审
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更新日期:2021-01-01 00:00:00
abstract::Forty-eight patients with adenocarcinoma of the gastrointestinal tract were treated on this trial. The MTD of 5-FU given as a 72 hour infusion with high-dose leucovorin was initially determined to be 2000 mg/m2/d. Patients were treated at PALA dose levels ranging from 250 to 2848 mg/m2. Biochemical assessment of targe...
journal_title:Advances in experimental medicine and biology
pub_type: 临床试验,杂志文章
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更新日期:1993-01-01 00:00:00