Insulin-like Growth Factor Binding Protein6 Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage in Rats.

Abstract:

:The insulin-like growth factor (IGF) system is linked to CNS pathological states. The functions of IGFs are modulated by a family of binding proteins termed insulin-like growth factor binding proteins (IGFBPs). Here, we demonstrate that IGFBP-6 may be associated with neuronal apoptosis in the processes of intracerebral hemorrhage (ICH). We obtained a significant upregulation of IGFBP-6 in neurons adjacent to the hematoma following ICH with the results of Western blot, immunohistochemistry, and immunofluorescence. Increasing IGFBP-6 level was found to be accompanied by the upregulation of Bax, Bcl-2, and active caspase-3. Besides, IGFBP-6 co-localized well with active caspase-3 in neurons, indicating its potential role in neuronal apoptosis. Knocking down IGFBP-6 by RNA-interference in PC12 cells reduced active caspase-3 expression. Thus, IGFBP-6 may play a role in promoting the brain secondary damage following ICH.

journal_name

Cell Mol Neurobiol

authors

Dai Q,Jiang P,Gu Y,Zhu L,Dai H,Yao Z,Liu H,Ma X,Duan C,Qu L

doi

10.1007/s10571-016-0453-1

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

1207-1216

issue

7

eissn

0272-4340

issn

1573-6830

pii

10.1007/s10571-016-0453-1

journal_volume

37

pub_type

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