Extracellular Matrix Glycoprotein-Derived Synthetic Peptides Differentially Modulate Glioma and Sarcoma Cell Migration.


:Glycoproteins of the extracellular matrix (ECM) regulate proliferation, migration, and differentiation in numerous cell lineages. ECM functions are initiated by small peptide sequences embedded in large constituents that are recognized by specific cellular receptors. In this study, we have investigated the biological effects of peptides derived from collagen type IV and tenascin-C compared to the well-known RGD peptide originally discovered in fibronectin. The influence of glycoproteins and corresponding peptides on the migration of the glioma cell lines U-251-MG and U-373-MG and the sarcoma line S-117 was studied. When the cell lines were tested in a modified Boyden chamber assay on filters coated with the ECM glycoproteins, glioma cells showed a strong migration response on tenascin-C and the basal lamina constituent collagen IV, in contrast to S-117 cells. In order to identify relevant stimulatory motifs, peptides derived from fibronectin (6NHX-GRGDSF), tenascin-C (TN-C, VSWRAPTA), and collagen type IV (MNYYSNS) were compared, either applied in solution in combination with ECM glycoprotein substrates, in solution in the presence of untreated membranes, or coated on the filters of the Boyden chambers. Using this strategy, we could identify the novel tenascin-C-derived peptide motif VSWRAPTA as a migration stimulus for glioma cells. Furthermore, while kin peptides generally blocked the effects of the respective homologous ECM proteins, unexpected effects were observed in heterologous situations. There, in several cases, addition of soluble peptides strongly boosted the response to the coated ECM proteins. We propose that peptides may synergize or antagonize each other by stimulating different signaling pathways.


Cell Mol Neurobiol


Brösicke N,Sallouh M,Prior LM,Job A,Weberskirch R,Faissner A




Has Abstract


2015-07-01 00:00:00












  • Response of substances co-expressed in hypothalamic magnocellular neurons to osmotic challenges in normal and Brattleboro rats.

    abstract::The intention of this review is to emphasize the current knowledge about the extent and importance of the substances co-localized with magnocellular arginine vasopressin (AVP) and oxytocin (OXY) as potential candidates for the gradual clarification of their actual role in the regulation of hydromineral homeostasis. Ma...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Bundzikova J,Pirnik Z,Zelena D,Mikkelsen JD,Kiss A

    更新日期:2008-12-01 00:00:00

  • Fos protein expression in sacral spinal cord in relation to early phase of cauda equina syndrome in dogs.

    abstract::1. The aim of the present study is to map the incipient phase of Fos expression in the sacral spinal cord neuronal pools of multiple cauda equina constrictions canine model. 2. Fos-positive neurons were found bilaterally in the lateral portion of superficial dorsal horn layers (Laminae I-III) and along the lateral edg...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Orendácová J,Marsala M,Cízková D,Kafka J,Raceková E,Sulla I,Vanický I,Marsala J

    更新日期:2001-08-01 00:00:00

  • Age-Dependency of Levetiracetam Effects on Exocytotic GABA Release from Nerve Terminals in the Hippocampus and Cortex in Norm and After Perinatal Hypoxia.

    abstract::Perinatal hypoxia can lead to multiple chronic neurological deficits, e.g., mental retardation, behavioral abnormalities, and epilepsy. Levetiracetam (LEV), 2S-(2-oxo-1-pyrrolidiny1) butanamide, is an anticonvulsant drug with proven efficiency in treating patients with focal and generalized seizures. Rats were underwe...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Pozdnyakova N,Dudarenko M,Borisova T

    更新日期:2019-07-01 00:00:00

  • Alterations of tubulin function caused by chronic antidepressant treatment in rat brain.

    abstract::1. Antidepressants have been used clinically for many years; however, the neurochemical mechanism for their therapeutic effect has not been clarified yet. Recent reports indicate that chronic antidepressant treatment directly affects the postsynaptic membrane to increase the coupling between the stimulatory GTP-bindin...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Kamada H,Saito T,Hatta S,Toki S,Ozawa H,Watanabe M,Takahata N

    更新日期:1999-02-01 00:00:00

  • Evidence for the production and action of interleukin-10 in pituitary cells.

    abstract::1. Interleukin-10 (IL-10) has a wide range of activities in the immune system such as modulation of interferon-gamma (IFN-gamma) and antibody production. The neuropeptide hormone corticotropin (ACTH) has similar activities, suggesting that a bidirectional communication mechanism operates between the immune and the neu...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Hughes TK,Cadet P,Rady PL,Tyring SK,Chin R,Smith EM

    更新日期:1994-02-01 00:00:00

  • EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells.

    abstract::1. Hypothalamic magnocellular neurons synthesize, store, and secrete large quantities of the neuropeptides, vasopressin (VP) and oxytocin (OT), which are synthesized as protein precursors also containing proteins called neurophysins. These protein precursors are sorted through the regulated secretory pathway (RSP), pa...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zhang BJ,Yamashita M,Fields R,Kusano K,Gainer H

    更新日期:2005-06-01 00:00:00

  • Evidence for site selection during synaptogenesis: the surface distribution of synaptic sites in photoreceptor terminals of the files Musca and Drosophila.

    abstract::1. Photoreceptor terminals in the flies Musca domestica and Drosophila melanogaster have been reconstructed in three dimensions from serial EM to reveal the surface distributions of afferent tetrad synapses. 2. The terminals are cylindrical and surround two target cells; they have synaptic sites distributed along thei...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Meinertzhagen IA,Hu X

    更新日期:1996-12-01 00:00:00

  • Functional coupling of G proteins to endothelin receptors is ligand and receptor subtype specific.

    abstract::1. The aims of the present study were (a) to determine the identity of the G proteins with which the endothelin receptor interacts and whether this interaction is subtype specific and (b) to determine whether agonist exposure can result in specific coupling between the endothelin receptor and G proteins. 2. Coupling b...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Shraga-Levine Z,Sokolovsky M

    更新日期:2000-06-01 00:00:00

  • DNA immunization against amyloid beta 42 has high potential as safe therapy for Alzheimer's disease as it diminishes antigen-specific Th1 and Th17 cell proliferation.

    abstract::The pathogenesis of Alzheimer's disease (AD) has been strongly associated with the accumulation of amyloid beta (Aβ) peptides in brain, and immunotherapy targeting Aβ provides potential for AD prevention. A clinical trial in which AD patients were immunized with Aβ42 peptide was stopped when 6% of participants showed ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Lambracht-Washington D,Qu BX,Fu M,Anderson LD Jr,Stüve O,Eagar TN,Rosenberg RN

    更新日期:2011-08-01 00:00:00

  • Involvement of Fra-1 in Retinal Ganglion Cell Apoptosis in Rat Light-Induced Retina Damage Model.

    abstract::Cell cycle re-entry, in which Fra-1 (transcription factor FOS-related antigen 1) plays an important role, is a key process in neuronal apoptosis. However, the expression and function of Fra-1 in retinal ganglion cell (RGC) apoptosis are unknown. To investigate whether Fra-1 was involved in RGC apoptosis, we performed ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Liu X,Yang X,Zhu R,Dai M,Zhu M,Shen Y,Fang H,Sang A,Chen H

    更新日期:2017-01-01 00:00:00

  • Mice with disrupted TGFbeta signaling have normal cerebella development, but exhibit facial dysmorphogenesis and strain-dependent deficits in their body wall.

    abstract::The transforming growth factor betas (TGFbetas) are context-dependent regulators of neurons in vitro, but their physiological functions in the brain are unclear. Haploinsufficiency of either Tgfbeta1 or Tgfbeta2 leads to age-related deterioration of neurons, but the development of the brain is normal in the full absen...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Asano Y,Koishi K,Frugier T,McLennan IS

    更新日期:2009-07-01 00:00:00

  • Increase of c-Fos and c-Jun expression in spinal and cranial motoneurons of the degenerating muscle mouse (Scn8a(dmu)).

    abstract::The degenerating muscle (dmu) mouse harbors a loss-of-function mutation in the Scn8a gene, which encodes the alpha subunit of the voltage-gated sodium channel (VGSC) Na(V)1.6. The distribution of c-Fos and c-Jun was examined in spinal and cranial motoneurons of the dmu mouse. In the cervical spinal cord, trigeminal mo...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Ichikawa H,Kano M,Shimizu Y,Suzuki T,Sawada E,Ono W,Chu LW,Côté PD

    更新日期:2010-07-01 00:00:00

  • Cholinesterases during development of the avian nervous system.

    abstract::1. Long before onset of synaptogenesis in the chicken neural tube, the closely related enzymes butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) are expressed in a mutually exclusive manner. Accordingly, neuroblasts on the ventricular side of the neural tube transiently express BChE before they abruptly acc...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Layer PG

    更新日期:1991-02-01 00:00:00

  • Early changes in behavior deficits, amyloid beta-42 deposits and MAPK activation in doubly transgenic mice co-expressing NSE-controlled human mutant PS2 and APPsw.

    abstract::1. Doubly transgenic mice were some differences in the period proceeding of the development of Abeta-42 deposits and behavioral deficits. It was not characterized human mutant PS2 (hPS2) with APPsw in the brains of double transgenic mice. The aim of this study was to examine whether doubly transgenic mice co-expressin...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Hwang DY,Cho JS,Oh JH,Shim SB,Jee SW,Lee SH,Seo SJ,Song CW,Lee SH,Kim YK

    更新日期:2005-08-01 00:00:00

  • Intracellular localization of the HCS2 gene products in identified snail neurons in vivo and in vitro.

    abstract::1. The HCS2 (Helix command specific 2) gene expressed in giant command neurons for withdrawal behavior of the terrestrial snail Helix lucorum encodes a unique hybrid precursor protein that contains a Ca-binding (EF-hand motif) protein and four small peptides (CNP1-CNP4) with similar Tyr-Pro-Arg-X aminoacid sequence at...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Ivanova JL,Leonova OG,Popenko VI,Ierusalimsky VN,Korshunova TA,Boguslavsky DV,Malyshev AY,Balaban PM,Belyavsky AV

    更新日期:2006-03-01 00:00:00

  • Insulin-like Growth Factor Binding Protein6 Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage in Rats.

    abstract::The insulin-like growth factor (IGF) system is linked to CNS pathological states. The functions of IGFs are modulated by a family of binding proteins termed insulin-like growth factor binding proteins (IGFBPs). Here, we demonstrate that IGFBP-6 may be associated with neuronal apoptosis in the processes of intracerebra...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Dai Q,Jiang P,Gu Y,Zhu L,Dai H,Yao Z,Liu H,Ma X,Duan C,Qu L

    更新日期:2017-10-01 00:00:00

  • Effect of Induction Time on the Proliferation and Differentiation of Induced Schwann-Like Cells from Adipose-Derived Stem Cells.

    abstract::To compare how different induction time takes effect on the proliferation and secretion ability of adipose-derived stem cell (ADSC)-induced Schwann-like cells (iSCs), ADSCs were isolated from healthy adult female rats. Flow cytometry (FCM) was performed to detect the ADSC-positive markers CD29, CD44, and CD90 and the ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Wong CW,Xu Y,Liu X,Xu S,Zhang Y,Zhu Z,He B

    更新日期:2020-10-01 00:00:00

  • Deficiency in endothelin receptor B reduces proliferation of neuronal progenitors and increases apoptosis in postnatal rat cerebellum.

    abstract::Endothelins regulate cellular functions in the mammalian brain through the endothelin receptors A and B (EDNRA and EDNRB). In this study, we investigated the role of EDNRB on cell proliferation in the cerebellum by using the spotting lethal (sl) rat, which carries a naturally occurring deletion in the EDNRB gene. Prol...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Vidovic M,Chen MM,Lu QY,Kalloniatis KF,Martin BM,Tan AH,Lynch C,Croaker GD,Cass DT,Song ZM

    更新日期:2008-12-01 00:00:00

  • Ischemia-induced mitochondrial apoptosis is significantly attenuated by ischemic preconditioning.

    abstract::Ischemic preconditioning (IPC) represents an important adaptation of CNS to sub-lethal ischemia, which results in increased tolerance of CNS to the lethal ischemia. Ischemia-induced mitochondrial apoptosis is considered to be an important event leading to neuronal cell death after cerebral blood flow arrest. In presen...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Racay P,Chomova M,Tatarkova Z,Kaplan P,Hatok J,Dobrota D

    更新日期:2009-09-01 00:00:00

  • 3-mercaptopropionic acid-induced seizures decrease NR2B expression in Purkinje cells: cyclopentyladenosine effect.

    abstract::Inhibitory mechanism of cerebellum epileptic activity can be involved depending on the intensity and frequency of seizure convulsions. N-methyl-D-aspartate receptors (NMDARs) play key roles in excitatory synaptic transmission and have been implicated in neurological disorders: in cerebellum, they have specific charact...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Girardi E,Auzmendi J,Charó N,Gori MB,Castro M

    更新日期:2010-10-01 00:00:00

  • Pre-clinical analysis of changes in intra-cellular biochemistry of glioblastoma multiforme (GBM) cells due to c-Myc silencing.

    abstract::Glioblastoma Multiforme (GBM) is an aggressive form of brain Tumor that has few cures. In this study, we analyze the anti-proliferative effects of a new molecule JQ1 against GBMs induced in Wistar Rats. JQ1 is essentially a Myc inhibitor. c-Myc is also known for altering the biochemistry of a tumor cell. Therefore, th...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Rajagopalan V,Vaidyanathan M,Janardhanam VA,Bradner JE

    更新日期:2014-10-01 00:00:00

  • Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.

    abstract::(1) A Little information exists on the distribution of Slit-Robo-GTPase activating proteins (srGAPs), particularly about their intracellular locations, which may provide further clues to their functions. The purpose of this study is to elucidate the expression patterns of the three srGAPs in wild-type rat brains at ad...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Yao Q,Jin WL,Wang Y,Ju G

    更新日期:2008-02-01 00:00:00

  • Effects of various kinds of stimulation on ornithine decarboxylase activity in superior cervical sympathetic and nodose ganglia of rats.

    abstract::Levels of cyclic nucleotides and ornithine decarboxylase (ODC) activity were examined following the application of various kinds of stimuli to superior cervical sympathetic ganglia (SCG), nodose ganglia, and vagus nerve fibers excised from the rat. The level of cyclic GMP in the SCG rose rapidly to about 4.5- to 7.5-f...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Ando M,Miwa M,Nagata Y

    更新日期:1984-03-01 00:00:00

  • Alterations in cerebrospinal fluid uridine, hypoxanthine, and xanthine in head-injured patients.

    abstract::1. Examination of the cerebrospinal fluid (CSF) of head-injured patients reveals that the concentration of intraventricular xanthine is elevated and that of uridine is decreased relative to those of adult lumbar CSF. 2. No correlations were observed between CSF lactate and CSF hypoxanthine, xanthine, or uridine, sugge...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Mueller RA,Rosner MJ,Ghia JN,Powers SK,Kafer ER,Hunt RD

    更新日期:1988-06-01 00:00:00

  • Protective effects of Arctium lappa L. roots against hydrogen peroxide-induced cell injury and potential mechanisms in SH-SY5Y cells.

    abstract::Accumulated evidence has shown that excessive reactive oxygen species (ROS) have been implicated in neuronal cell death related with various chronic neurodegenerative disorders. This study was designed to explore neuroprotective effects of ethyl acetate extract of Arctium lappa L. roots (EAL) on hydrogen peroxide (H2O...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Tian X,Guo LP,Hu XL,Huang J,Fan YH,Ren TS,Zhao QC

    更新日期:2015-04-01 00:00:00

  • Glial cells are involved in the exciting effects of doxapram on brainstem slices in vitro.

    abstract::This study tested whether the glial cells are involved in the exciting effects of doxapram on brainstem slice in vitro. Experiments were performed in brainstem slice preparations from neonatal rats. The medial area of nucleus retrofacialis (mNRF) and the hypoglossal nerve (XII nerve) were contained in the preparations...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Li GC,Zhang HT,Jiao YG,Wu ZH,Fang F,Cheng J

    更新日期:2010-07-01 00:00:00

  • Complexity of Compensatory Effects in Nrf1 Knockdown: Linking Undeveloped Anxiety-Like Behavior to Prevented Mitochondrial Dysfunction and Oxidative Stress.

    abstract::Anxiety-related disorders are complex illnesses that underlying molecular mechanisms need to be understood. Mitochondria stand as an important link between energy metabolism, oxidative stress, and anxiety. The nuclear factor, erythroid-derived 2,-like 1(Nrf1) is a member of the cap "n" collar subfamily of basic region...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Khalifeh S,Oryan S,Khodagholi F,Digaleh H,Shaerzadeh F,Maghsoudi N,Zarrindast MR

    更新日期:2016-05-01 00:00:00

  • S100, a brain-specific protein: localization and possible role in the snail nervous system.

    abstract::Immunofluorescence techniques were used to show that S100 is present on the surface of neuronal and glial membranes of Helix pomatia in vitro. By the method of rocket immunoelectrophoresis of aqueous , Trition, and n-pentanol extracts of snail nervous tissue, S100 was demonstrated to be mainly in the membrane fraction...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Shtark MB,Gainutdinov KL,Khichenko VI,Starostina MV

    更新日期:1981-09-01 00:00:00

  • Adoptive Regulatory T-cell Therapy Attenuates Perihematomal Inflammation in a Mouse Model of Experimental Intracerebral Hemorrhage.

    abstract::The CD4+CD25+ regulatory T cells (Tregs), an innate immunomodulator, suppress cerebral inflammation and maintain immune homeostasis in multiple central nervous system injury, but its role in intracerebral hemorrhage (ICH) has not been fully characterized. This study investigated the effect of Tregs on brain injury usi...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Mao LL,Yuan H,Wang WW,Wang YJ,Yang MF,Sun BL,Zhang ZY,Yang XY

    更新日期:2017-07-01 00:00:00

  • Impact of Astrocytes on the Injury Induced by In Vitro Ischemia.

    abstract::Cell cultures are characterized by their simplicity, controllability, and ability to provide detailed basic information on how a particular cell population responds to specific stimuli or insult. These characteristics led to their extensive application in the study of molecular interactions and represent a valuable to...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Roque C,Baltazar G

    更新日期:2017-11-01 00:00:00