Extracellular Matrix Glycoprotein-Derived Synthetic Peptides Differentially Modulate Glioma and Sarcoma Cell Migration.


:Glycoproteins of the extracellular matrix (ECM) regulate proliferation, migration, and differentiation in numerous cell lineages. ECM functions are initiated by small peptide sequences embedded in large constituents that are recognized by specific cellular receptors. In this study, we have investigated the biological effects of peptides derived from collagen type IV and tenascin-C compared to the well-known RGD peptide originally discovered in fibronectin. The influence of glycoproteins and corresponding peptides on the migration of the glioma cell lines U-251-MG and U-373-MG and the sarcoma line S-117 was studied. When the cell lines were tested in a modified Boyden chamber assay on filters coated with the ECM glycoproteins, glioma cells showed a strong migration response on tenascin-C and the basal lamina constituent collagen IV, in contrast to S-117 cells. In order to identify relevant stimulatory motifs, peptides derived from fibronectin (6NHX-GRGDSF), tenascin-C (TN-C, VSWRAPTA), and collagen type IV (MNYYSNS) were compared, either applied in solution in combination with ECM glycoprotein substrates, in solution in the presence of untreated membranes, or coated on the filters of the Boyden chambers. Using this strategy, we could identify the novel tenascin-C-derived peptide motif VSWRAPTA as a migration stimulus for glioma cells. Furthermore, while kin peptides generally blocked the effects of the respective homologous ECM proteins, unexpected effects were observed in heterologous situations. There, in several cases, addition of soluble peptides strongly boosted the response to the coated ECM proteins. We propose that peptides may synergize or antagonize each other by stimulating different signaling pathways.


Cell Mol Neurobiol


Brösicke N,Sallouh M,Prior LM,Job A,Weberskirch R,Faissner A




Has Abstract


2015-07-01 00:00:00












  • Neuronal analysis and behaviour in prenatally gamma-irradiated rats.

    abstract::The intrauterinal development in mammals represents a very sensitive period of life in relation to many environmental factors, including ionizing radiation (IR). The developing nervous system is particularly vulnerable to IR, and the consequences of exposure are of importance because of its potential health risks. The...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Kokošová N,Tomášová L,Kisková T,Šmajda B

    更新日期:2015-01-01 00:00:00

  • Effect of NMDA-induced lesion of the subfornical organ on the angiotensin II binding sites density and acetylcholinesterase or NADPH-diphorase activities in the lamina terminalis of the rat brain.

    abstract::1. Neural angiotensinergic circuitry located in the lamina terminalis has been proposed to be involved in blood pressure regulation and fluid homeostasis. 2. ANG II binding sites have been described to be localized throughout the lamina terminalis including the subfornical organ (SFO), the median preoptic nucleus (MnP...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Guilhaume SS,Corrêa FM

    更新日期:2001-02-01 00:00:00

  • Regulation of the biosynthesis of large dense-core vesicles in chromaffin cells and neurons.

    abstract::1. The proteins of large dense-core vesicles (LDV) in neuroendocrine tissues are well characterized. Secretory components comprise chromogranins and neuropeptides. Intrinsic membrane proteins include cytochrome b-561, transporters, SV2, synaptotagmin, and synaptobrevin. 2. The effects of stimulation and of second mess...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Winkler H,Fischer-Colbrie R

    更新日期:1998-04-01 00:00:00

  • Enhanced slow inactivation of the human skeletal muscle sodium channel causing normokalemic periodic paralysis.

    abstract::Normokalemic periodic paralysis (normoPP) is a type of skeletal muscle function disorder which is characterized by paralysis attack with concomitant normal serum potassium level. We previously reported that R675Q mutation of human skeletal muscle voltage-gated sodium channel α subunit (SCN4A) may be the novel mutation...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Wu L,Zhang B,Kang Y,Wu W

    更新日期:2014-07-01 00:00:00

  • Effects of phytoestrogen on mitochondrial structure and function of hippocampal CA1 region of ovariectomized rats.

    abstract::The present study was undertaken to evaluate whether estrogen deprivation might lead to mitochondrial alteration of hippocampal neurons of ovariectomized (OVX) rats, and to evaluate the protective effect of estrogen and phytoestrogen on the mitochondrial alteration. First, OVX rats were used to mimic the pathologic ch...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Xu XW,Shi C,He ZQ,Ma CM,Chen WH,Shen YP,Guo Q,Shen CJ,Xu J

    更新日期:2008-09-01 00:00:00

  • Dynamic change of Prohibitin2 expression in rat sciatic nerve after crush.

    abstract::As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (S...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Long L,Huang Y,Wu H,Luan W,Zhang Q,Wen H,Ding T,Wang Y

    更新日期:2013-07-01 00:00:00

  • Impact of Astrocytes on the Injury Induced by In Vitro Ischemia.

    abstract::Cell cultures are characterized by their simplicity, controllability, and ability to provide detailed basic information on how a particular cell population responds to specific stimuli or insult. These characteristics led to their extensive application in the study of molecular interactions and represent a valuable to...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Roque C,Baltazar G

    更新日期:2017-11-01 00:00:00

  • Involvement of spinal chemokine CCL2 in the hyperalgesia evoked by bone cancer in mice: a role for astroglia and microglia.

    abstract::The hypernociceptive role played by the chemokine CCL2, and its main receptor, CCR2, in pathological settings is being increasingly recognized. We aimed to characterize the involvement of spinal CCL2 in the hyperalgesia due to the intratibial inoculation of fibrosarcoma NCTC 2472 cells in mice. The intrathecal (i.t.) ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Pevida M,González-Rodríguez S,Lastra A,García-Suárez O,Hidalgo A,Menéndez L,Baamonde A

    更新日期:2014-01-01 00:00:00

  • Structural insights into the function of P2X4: an ATP-gated cation channel of neuroendocrine cells.

    abstract::The P2X4 receptor (P2X4R) is a member of a family of ATP-gated cation channels that are composed of three subunits. Each subunit has two transmembrane (TM) domains linked by a large extracellular loop and intracellularly located N- and C-termini. The receptors are expressed in excitable and non-excitable cells and hav...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Stojilkovic SS,Yan Z,Obsil T,Zemkova H

    更新日期:2010-11-01 00:00:00

  • Effect of cholinomimetics on the release and uptake of L-[3H] glutamic acid in rat neostriatum.

    abstract::The effects of cholinomimetics on the release and uptake of L-glutamic acid have been studied by means of local superfusion of rat neostriatum (in vivo) and using striatum slices (in vitro). Cholinomimetics regulate the release of L-glutamic acid through both muscarinic (m-) and nicotinic (n-) choline receptors accord...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Godukhin OV,Budantsev AYu,Selifanova OV,Agapova VN

    更新日期:1984-06-01 00:00:00

  • Aquaporin-mediated fluid regulation in the inner ear.

    abstract::1. The sensory functions of the inner ear (hearing and balance) critically depend on the precise regulation of two fluid compartments of highly desparate ion composition, i.e., the endolymph and the perilymph. 2. The parameters volume, ion composition, and pH need to be held at homeostasis irrespective of the hydratio...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Beitz E,Zenner HP,Schultz JE

    更新日期:2003-06-01 00:00:00

  • Metabolism and pharmacokinetics of selective serotonin reuptake inhibitors.

    abstract::1. Five drugs with the predominant pharmacologic effect of inhibiting the neuronal reuptake of serotonin are available worldwide for clinical use. This class of psychoactive drugs, known as selective serotonin reuptake inhibitors (SSRIs), is comprised of fluoxetine, sertraline, paroxetine, fluvoxamine, and citalopram....

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: DeVane CL

    更新日期:1999-08-01 00:00:00

  • Intrinsically disordered proteins in the neurodegenerative processes: formation of tau protein paired helical filaments and their analysis.

    abstract::1. Several intrinsically disordered proteins (IDPs) play principal role in the neurodegenerative processes of various types. Among them, alpha-synuclein is involved in Parkinson's disease, prion protein in transmissible spongiform encephalopathies, and tau protein in Alzheimer's disease (AD) and related tauopathies. N...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Skrabana R,Sevcik J,Novak M

    更新日期:2006-10-01 00:00:00

  • Predominant neuritic pathology induced by viral overexpression of alpha-synuclein in cell culture.

    abstract::1. Alpha-synuclein is known to play an important role in the pathogenesis of Parkinson's disease (PD). The pathogenicity of alpha-synuclein is related to its ability to form intraneuronal inclusions. The inclusions, which are found in brains of patients with PD and diffuse Lewy body disease consist partially of C-term...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zach S,Bueler H,Hengerer B,Gillardon F

    更新日期:2007-06-01 00:00:00

  • Function of Cathepsin K in the Central Nervous System of Male Mice is Independent of Its Role in the Thyroid Gland.

    abstract::Cathepsin K deficiency in male mice (Ctsk-/-) results in decreased numbers of hippocampal astrocytes and altered neuronal patterning as well as learning and memory deficits. Additionally, cathepsin K carries essential roles in the thyroid gland where it contributes to the liberation of thyroid hormones (TH). Because T...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Dauth S,Rakov H,Sîrbulescu RF,Ilieş I,Weber J,Batbajar Dugershaw B,Braun D,Rehders M,Wirth EK,Führer D,Schweizer U,Brix K

    更新日期:2020-07-01 00:00:00

  • Chromogranin A as a crucial factor in the sorting of peptide hormones to secretory granules.

    abstract::Chromogranin A (CgA) is a soluble glycoprotein stored along with hormones and neuropeptides in secretory granules of endocrine cells. In the last four decades, intense efforts have been concentrated to characterize the structure and the biological function of CgA. Besides, CgA has been widely used as a diagnostic mark...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Elias S,Delestre C,Courel M,Anouar Y,Montero-Hadjadje M

    更新日期:2010-11-01 00:00:00

  • Naturally occurring genetic variants in human chromogranin A (CHGA) associated with hypertension as well as hypertensive renal disease.

    abstract::Chromogranin A (CHGA) plays a fundamental role in the biogenesis of catecholamine secretory granules. Changes in storage and release of CHGA in clinical and experimental hypertension prompted us to study whether genetic variation at the CHGA locus might contribute to alterations in autonomic function, and hence hypert...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Chen Y,Rao F,Wen G,Gayen JR,Zhang K,Vaingankar SM,Biswas N,Mahata M,Friese RS,Fung MM,Salem RM,Nievergelt C,Bhatnagar V,Hook VY,Ziegler MG,Mahata SK,Hamilton BA,O'Connor DT

    更新日期:2010-11-01 00:00:00

  • The Regulation of GluN2A by Endogenous and Exogenous Regulators in the Central Nervous System.

    abstract::The NMDA receptor is the most widely studied ionotropic glutamate receptor, and it is central to many physiological and pathophysiological processes in the central nervous system. GluN2A is one of the two main types of GluN2 NMDA receptor subunits in the forebrain. The proper activity of GluN2A is important to brain f...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Sun Y,Zhan L,Cheng X,Zhang L,Hu J,Gao Z

    更新日期:2017-04-01 00:00:00

  • Effects of altered expression and localization of cyclophilin A on differentiation of p19 embryonic carcinoma cells.

    abstract::1. The retinoblastoma susceptibility gene product, p105Rb (RB), is an important regulator in the control of cell proliferation, differentiation, and apoptosis. Several cellular factors that complex with RB and exert their cellular regulatory functions have been identified, such as the RB:cyclophilin A (CypA) complex. ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Chiu R,Rey O,Zheng JQ,Twiss JL,Song J,Pang S,Yokoyama KK

    更新日期:2003-12-01 00:00:00

  • Correlations between cholinesterase activity and cognitive scores in post-ischemic rats and patients with vascular dementia.

    abstract::The biochemical changes such as the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were investigated in rats with global cerebral ischemia and in vascular dementia (VaD) subjects in this study. The AChE activity showed a significant decrease in plasma and a significant increase in the hipp...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Xiao Y,Guan ZZ,Wu CX,Li Y,Kuang SX,Pei JJ

    更新日期:2012-04-01 00:00:00

  • Thyroid hormone mediates syndecan expression in rat neonatal cerebellum.

    abstract::Thyroid hormone (T(3)) plays an essential role in the central nervous system development. Astrocytes mediate many of the T(3) effects in the growth and differentiation of cerebellum. In culture, T(3) induces cerebellar astrocytes to secrete growth factors, mainly FGF(2), and alters the expression and organization of t...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Mendes-de-Aguiar CB,Costa-Silva B,Alvarez-Silva M,Tasca CI,Trentin AG

    更新日期:2008-09-01 00:00:00

  • Dopamine: Functions, Signaling, and Association with Neurological Diseases.

    abstract::The dopaminergic system plays important roles in neuromodulation, such as motor control, motivation, reward, cognitive function, maternal, and reproductive behaviors. Dopamine is a neurotransmitter, synthesized in both central nervous system and the periphery, that exerts its actions upon binding to G protein-coupled ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,评审


    authors: Klein MO,Battagello DS,Cardoso AR,Hauser DN,Bittencourt JC,Correa RG

    更新日期:2019-01-01 00:00:00

  • Altered Sodium and Potassium, but not Calcium Currents in Cerebellar Granule Cells in an In Vitro Model of Neuronal Injury.

    abstract::Acute injury of central nervous system (CNS) starts a cascade of morphological, molecular, and functional changes including formation of a fibrotic scar, expression of transforming growth factor beta 1 (TGF-β1), and expression of extracellular matrix proteins leading to arrested neurite outgrowth and failed regenerati...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Ondáčová K,Jurkovičová D,Lacinová Ľ

    更新日期:2017-07-01 00:00:00

  • Correction to: Pigment Epithelium-Derived Factor Improves Paracellular Blood-Brain Barrier Integrity in the Normal and Ischemic Mouse Brain.

    abstract::The original version of the article unfortunately contained an error in the unit of the protein concentrations under 'Stereotactic Intraparenchymal Injections' subsection in 'Methods' section. ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章,已发布勘误


    authors: Riabinska A,Zille M,Terzi MY,Cordell R,Nieminen-Kelhä M,Klohs J,Piña AL

    更新日期:2020-07-01 00:00:00

  • Shengmai Formula Ameliorates Pathological Characteristics in AD C. elegans.

    abstract::Shengmai (SM) formula, a classical traditional Chinese medicine formula, is composed of Panax ginseng (Pg), Ophiopogon japonicus (Oj), and Schisandra Chinesis (Sc). SM has been clinically used to treat heart failure and ischemic heart disease. Although SM formula has been reported to be potential for fighting against ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Zhang W,Zhi D,Ren H,Wang D,Wang X,Zhang Z,Fei D,Zhu H,Li H

    更新日期:2016-11-01 00:00:00

  • Persistent calcium inward current in internally perfused snail neuron.

    abstract::The inactivation process of the calcium current (ICa) was investigated in a molluscan neuron which was perfused intracellularly and voltage-clamped using a suction pipette technique. The decay phase of the ICa contained a very slowly inactivated component (persistent inward current; PIC). The decay time constant of th...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Oyama Y,Akaike N,Nishi K

    更新日期:1986-03-01 00:00:00

  • Accumulation of different c-erbA transcripts during rat brain development and in cortical neurons cultured in a synthetic medium.

    abstract::1. Accumulation of different c-erbA transcripts was studied, during rat brain maturation and in cortical neurons differentiating in a serum-free medium, by quantitative Northern blot hybridization. 2. The alpha and beta forms of c-erbA mRNAs exhibit different patterns of accumulation, with a precocious increase in the...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Castiglia D,Cestelli A,Di Liegro C,Bonfanti L,Di Liegro I

    更新日期:1992-06-01 00:00:00

  • Expression of P2X3 and P2X 5 myenteric receptors varies during the intestinal postnatal development in the guinea pig.

    abstract::P2X3 receptor expression in various tissues appears to be modulated by age. In the present study, we used single cell RT-PCR to determine the number of P2X3 positive myenteric neurons at different stages of guinea pig postnatal development, and we tested if similar changes also occur to other myenteric P2X receptors. ...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Loera-Valencia R,Jiménez-Vargas NN,Villalobos EC,Juárez EH,Lomas-Ramos TL,Espinosa-Luna R,Montaño LM,Huizinga JD,Barajas-López C

    更新日期:2014-07-01 00:00:00

  • Pulsed radiofrequency reduced complete Freund's adjuvant-induced mechanical hyperalgesia via the spinal c-Jun N-terminal kinase pathway.

    abstract::Pulsed radiofrequency (PRF) treatment involves the pulsed application of a radiofrequency electric field to a nerve. The technology offers pain relief for patients suffering from chronic pain who do not respond well to conventional treatments. We tested whether PRF treatment attenuated complete Freund's adjuvant (CFA)...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Chen KH,Yang CH,Juang SE,Huang HW,Cheng JK,Sheen-Chen SM,Cheng JT,Lin CR

    更新日期:2014-03-01 00:00:00

  • Exocytosis in single chromaffin cells: regulation by a secretory granule-associated Go protein.

    abstract::1. Besides having a role in signal transduction, trimeric G proteins may also be involved in membrane trafficking events. In chromaffin cells, G alpha o has been found associated with the membrane of secretory granules. Here we examined the role of Go in regulated exocytosis using pressure microinjection combined with...

    journal_title:Cellular and molecular neurobiology

    pub_type: 杂志文章


    authors: Vitale N,Gonon F,Thiersé D,Aunis D,Bader MF

    更新日期:1997-02-01 00:00:00