Abstract：
:The treatment of Alzheimer's disease (AD) has been hampered by a lack of sensitive and specific non-invasive diagnostic methods. Quantum dots (QD) are nano-crystals with unique photo-physical properties that bypass some of the limitations of conventional dyes and imaging tools. This study is aimed to evaluate the fluorescence properties of a QD probe conjugated with an anti-Aβ antibody (QD-Aβ-Ab). Healthy mice and mice bearing mutated human APP695swe and APP717 V-F transgenes received intracerebroventricular injection of the probe for subsequent imaging. Immunohistochemistry revealed that Aβ1-42 was distributed in the hippocampus CA1 area in the APP transgenic mice. Fluorescence microscopy demonstrated that fluorescence was mainly observed in the hippocampus area, the cerebral cortex, sagittal septum and striatum of APP transgenic mice. In vivo imaging of mice receiving the QD-Aβ-Ab probe showed that healthy mice exhibited a narrow range of fluorescence and lower fluorescence intensity compared with APP transgenic mice. The mean fluorescence intensity of brain tissues of healthy C57BL mice was 12.3784 ± 3.9826, which was significantly lower than that of 10- and 16-month-old APP transgenic mice (45.03 ± 2.66 and 46.69 ± 3.22, respectively; P < 0.05). In this study we present the first direct evidence that QD-Aβ-Ab conjugate probes can track in vivo state of Aβ accumulation in mice and the findings suggest that such probes may be of potential use for early molecular diagnostic imaging of AD.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Feng L,Long HY,Liu RK,Sun DN,Liu C,Long LL,Li Y,Chen S,Xiao Bdoi
10.1007/s10571-013-9943-6subject
Has Abstractpub_date
2013-08-01 00:00:00pages
759-65issue
6eissn
0272-4340issn
1573-6830journal_volume
33pub_type
杂志文章abstract：:We previously reported that inhibition of Rho-kinase (ROCK) by hydroxyl fasudil improves cognitive deficit and neuronal damage in rats with chronic cerebral ischemia (Huang et al., Cell Mol Neurobiol 28:757-768, 2008). In this study, fasudil mesylate (FM) was investigated for its neuroprotective potential in rats with...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-008-9308-8
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abstract：:Compound 511 (511) is specially developed for opioid addiction treatment based on the Ancient Chinese drug rehabilitation literature, and its composition has profound effects in the treatment of drug addiction in various clinical trials and animal experiments. The effect of 511 on the rewarding properties of morphine ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-020-00848-9
更新日期：2020-04-22 00:00:00
abstract：:1. The original concept of the ischemic penumbra surrounding a focus of dense cerebral ischemia is based on electrophysiological observations. In the cortex of baboons following middle cerebral artery occlusion, complete failure of the cortical evoked potential was observed at a cerebral blood flow (CBF) threshold lev...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1023/a:1020629818207
更新日期：1998-12-01 00:00:00
abstract：:Spinal cord libraries subtracted against visual cortex using suppression subtractive hybridization SSH are dominated by abundant gene sequences derived from myelin elements. We compared our subtracted library results of three of these abundant sequences to published expressed sequence tag libraries that are not normal...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9408-0
更新日期：2009-12-01 00:00:00
abstract：:Metastasis of lung cancer to the brain is associated with poor outcomes and limited therapeutic options. The blood-brain barrier (BBB) plays a major role in brain metastasis. However, little is known about the role of pericytes in brain metastasis formation. This study aimed to reveal the interaction between pericytes...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00725-0
更新日期：2020-01-01 00:00:00
abstract：:Immunofluorescence techniques were used to show that S100 is present on the surface of neuronal and glial membranes of Helix pomatia in vitro. By the method of rocket immunoelectrophoresis of aqueous , Trition, and n-pentanol extracts of snail nervous tissue, S100 was demonstrated to be mainly in the membrane fraction...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00710684
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0172-z
更新日期：2015-08-01 00:00:00
abstract：:The effects of cholinomimetics on the release and uptake of L-glutamic acid have been studied by means of local superfusion of rat neostriatum (in vivo) and using striatum slices (in vitro). Cholinomimetics regulate the release of L-glutamic acid through both muscarinic (m-) and nicotinic (n-) choline receptors accord...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00710999
更新日期：1984-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9427-x
更新日期：2010-01-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9004-5
更新日期：2006-07-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-020-00922-2
更新日期：2020-07-18 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0161-2
更新日期：2015-07-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-020-00836-z
更新日期：2021-01-01 00:00:00
abstract：:1. 125I-Endothelin (ET)-1 binding to the rat anterior pituitary gland was saturable and single, with a Kd of 71 pM and a Bmax of 120 fmol/mg. 2. When 1.0 microM BQ-123 (ETA antagonist) was added to the incubation buffer, the binding parameters were 8.3 pM and 8.0 fmol/mg, whereas 10 nM sarafotoxin S6c (ETB agonist) ex...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1022557717481
更新日期：1998-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1023/a:1006934807375
更新日期：1999-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02088713
更新日期：1994-08-01 00:00:00
abstract：:1. Chitin is known to promote skin wound healing. In this study, chitin, prepared from Zuwai crab shell, was used as a bridge between the proximal and distal stumps of cut hypoglossal nerves in shrews. We compared the effects of chitin on the regeneration of transected right hypoglossal nerve axons, with those of porc...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1007055626632
更新日期：2000-10-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9037-9
更新日期：2006-10-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02152058
更新日期：1996-10-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9618-5
更新日期：2011-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1007125413308
更新日期：2001-02-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/BF00711177
更新日期：1990-09-01 00:00:00
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journal_title：Cellular and molecular neurobiology
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doi：10.1007/s10571-010-9565-1
更新日期：2010-11-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00765-6
更新日期：2020-07-01 00:00:00
abstract：:1. Nitric oxide (NO) is thought to play a neuromodulatory role in the nervous system of vertebrate and invertebrate species. In the hornworm Manduca sexta, NO-mediated signaling has been implicated in behavioral and developmental processes, but its exact function in neurons is unknown. In this study, we identify speci...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9091-3
更新日期：2007-03-01 00:00:00
abstract：:Chromogranin A (CHGA) plays a fundamental role in the biogenesis of catecholamine secretory granules. Changes in storage and release of CHGA in clinical and experimental hypertension prompted us to study whether genetic variation at the CHGA locus might contribute to alterations in autonomic function, and hence hypert...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9600-2
更新日期：2010-11-01 00:00:00
abstract：:(1) Following acute spinal cord injury, progesterone modulates several molecules essential for motoneuron function, although the morphological substrates for these effects are unknown. (2) The present study analyzed morphological changes in motoneurons distal to the lesion site from rats with or without progesterone t...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-008-9291-0
更新日期：2009-02-01 00:00:00
abstract：:Calmodulin is known to transduce Ca(2+) signals by interacting with specific target proteins. In order to determine the role of calmodulin in regulating neuronal survival and death, we examined, whether calmodulin inhibitors induce caspase-dependent apoptotic cell death, and whether glycogen synthase kinase-3 is invol...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-007-9172-y
更新日期：2007-09-01 00:00:00
abstract：:1. The effect of lead (in vivo) on the uptake of GABA, dopamine, and histidine as a precursor of histamine in synaptosomes obtained from chronically lead-treated rats was studied. 2. Lead decreased the uptake of GABA, increased the uptake of dopamine, and did not change the uptake of histidine. These effects were inde...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02088678
更新日期：1994-12-01 00:00:00
abstract：:The biochemical changes such as the activities of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were investigated in rats with global cerebral ischemia and in vascular dementia (VaD) subjects in this study. The AChE activity showed a significant decrease in plasma and a significant increase in the hipp...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-011-9770-6
更新日期：2012-04-01 00:00:00
