Abstract：
:Arachidonic acid (ARA) is one of the most abundant polyunsaturated fatty acids (PUFAs) in the mammalian brain. Many enzymatically- and nonenzymatically-produced metabolic products have important and potent pharmacological properties. However, uniformly isotope labeled forms of ARA are not commercially available for studying the metabolic fates of ARA. This study describes a simple and efficient protocol for the biosynthesis of U-13C-ARA from U-13C-glucose, and U-14C-ARA from U-14C-glucose by Mortierella alpina. The protocols yield approximately 100nmol quantities of U-13C-ARA with an isotopic purity of 95% from a 500μl batch volume, and approximately 2μCi quantities of U-14C-ARA with an apparent specific activity in excess of 1200Ci/mol from a 250μl batch volume.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Lee JV,Furman R,Axelsen PHdoi
10.1016/j.biortech.2016.12.050subject
Has Abstractpub_date
2017-03-01 00:00:00pages
142-146eissn
0960-8524issn
1873-2976pii
S0960-8524(16)31720-5journal_volume
227pub_type
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