Abstract:
:In this study, effects of temperature, inducer concentration, time of induction and co-expression of molecular chaperones (GroEL-GroES and DnaKJE), on cell growth and solubilization of model protein, xylanases, were investigated. The yield of soluble xylanases increased with decreasing cultivation temperature and inducer level. In addition, co-expression of DnaKJE chaperone resulted in increased soluble xylanases though the time of induction of chaperone and target protein had a bearing on this yield. A combination of chaperone co-expression and partial induction resulted in ∼40% (in DnaKJE) and 33% (in GroEL-GroES) of total xylanase yield in soluble fraction. However, the conditions for maximum yield of soluble r-XynB and maximum % soluble expression of r-XynB were different. Higher expression of soluble xylanases in a scalable semi-synthetic medium showed potential of the process for soluble enzyme production.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Jhamb K,Sahoo DKdoi
10.1016/j.biortech.2012.07.011subject
Has Abstractpub_date
2012-11-01 00:00:00pages
135-43eissn
0960-8524issn
1873-2976pii
S0960-8524(12)01058-9journal_volume
123pub_type
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