Production of soluble recombinant proteins in Escherichia coli: effects of process conditions and chaperone co-expression on cell growth and production of xylanase.

Abstract:

:In this study, effects of temperature, inducer concentration, time of induction and co-expression of molecular chaperones (GroEL-GroES and DnaKJE), on cell growth and solubilization of model protein, xylanases, were investigated. The yield of soluble xylanases increased with decreasing cultivation temperature and inducer level. In addition, co-expression of DnaKJE chaperone resulted in increased soluble xylanases though the time of induction of chaperone and target protein had a bearing on this yield. A combination of chaperone co-expression and partial induction resulted in ∼40% (in DnaKJE) and 33% (in GroEL-GroES) of total xylanase yield in soluble fraction. However, the conditions for maximum yield of soluble r-XynB and maximum % soluble expression of r-XynB were different. Higher expression of soluble xylanases in a scalable semi-synthetic medium showed potential of the process for soluble enzyme production.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Jhamb K,Sahoo DK

doi

10.1016/j.biortech.2012.07.011

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

135-43

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(12)01058-9

journal_volume

123

pub_type

杂志文章
  • Removal of oxytetracycline (OTC) in a synthetic pharmaceutical wastewater by a sequential anaerobic multichamber bed reactor (AMCBR)/completely stirred tank reactor (CSTR) system: biodegradation and inhibition kinetics.

    abstract::An anaerobic multichamber bed reactor (AMCBR) was effective in removing both molasses-chemical oxygen demand (COD), and the antibiotic oxytetracycline (OTC). The maximum COD and OTC removals were 99% in sequential AMCBR/completely stirred tank reactor (CSTR) at an OTC concentration of 300 mg L(-1). 51%, 29% and 9% of ...

    journal_title:Bioresource technology

    pub_type: 杂志文章,收录出版

    doi:10.1016/j.biortech.2011.10.056

    authors: Sponza DT,Çelebi H

    更新日期:2012-01-01 00:00:00

  • Enhanced adsorption of phenolic compounds, commonly encountered in olive mill wastewaters, on olive husk derived activated carbons.

    abstract::Olive husk was used for the preparation of activated carbon by chemical activation with KOH. The effects of carbonization and activation time on carbon properties were evaluated. The surface area of the produced carbons was measured by means of N(2) adsorption at 77K. The carbons with the highest surface area were fur...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.11.057

    authors: Michailof C,Stavropoulos GG,Panayiotou C

    更新日期:2008-09-01 00:00:00

  • A new approach to study the decolorization of complex reactive dye bath effluent by biosorption technique.

    abstract::This work focused on the development of a practical biosorbent for the decolorization of textile effluents. The fermentation waste, Corynebacterium glutamicum biomass, when decarboxylated and immobilized in polysulfone matrix performed well in decolorization of simulated reactive dye bath effluent comprised of four di...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.10.012

    authors: Vijayaraghavan K,Lee MW,Yun YS

    更新日期:2008-09-01 00:00:00

  • Fatty acid shifts and metabolic activity changes of Schizochytrium sp. S31 cultured on glycerol.

    abstract::DHA production by Schizochytrium sp. S31 was studied in batch cultures on glycerol with stepwise dissolved oxygen strategy. Three growth stages were identified as cell growth, lipid accumulation and lipid turnover. It was revealed that fatty acid (FA) shifts during the three growth stages involved the activity changes...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.05.030

    authors: Chang G,Luo Z,Gu S,Wu Q,Chang M,Wang X

    更新日期:2013-08-01 00:00:00

  • Supercritical CO2 pretreatment of lignocellulose enhances enzymatic cellulose hydrolysis.

    abstract::The supercritical carbon dioxide (SC-CO2) pretreatment of lignocellulose for enzymatic hydrolysis of cellulose was investigated. Aspen (hardwood) and southern yellow pine (softwood) with moisture contents in the range of 0-73% (w/w) were pretreated with SC-CO2 at 3100 and 4000 psi and at 112-165 degrees C for 10-60 mi...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/s0960-8524(00)00147-4

    authors: Kim KH,Hong J

    更新日期:2001-04-01 00:00:00

  • Pretreatment of yellow pine in an acidic ionic liquid: extraction of hemicellulose and lignin to facilitate enzymatic digestion.

    abstract::The acidic ionic liquid 1-H-3-methylimidazolium chloride can effectively pretreat yellow pine wood chips under mild conditions for enzymatic saccharification. Wood samples were treated at temperatures between 110 and 150°C for up to 5 h in the ionic liquid and three fractions collected; a cellulose rich fraction, lign...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.01.081

    authors: Cox BJ,Ekerdt JG

    更新日期:2013-04-01 00:00:00

  • Characteristics of hydrogen production and substrate consumption of Rhodopseudomonas palustris CQK 01 in an immobilized-cell photobioreactor.

    abstract::Effects of operation parameters on hydrogen production rate, substrate utilization efficiency and hydrogen bioconversion yield were investigated in a photobioreactor packed with sodium alginate/polyvinyl alcohol-124/carrageenan granules containing Rhodopseudomonas palustris CQK 01. Results demonstrate that an increase...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.01.045

    authors: Wang YZ,Liao Q,Zhu X,Tian X,Zhang C

    更新日期:2010-06-01 00:00:00

  • Salinity enhances high optically active L-lactate production from co-fermentation of food waste and waste activated sludge: Unveiling the response of microbial community shift and functional profiling.

    abstract::Lactic acid (LA), a versatile platform molecule, can be fermented from organic wastes, such as food waste and waste activated sludge. In this study, an efficient approach using salt, a component of food waste as an additive, was proposed to increase LA production. The LA productivity was increased at 10 g NaCl/L and o...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2020.124124

    authors: Li X,Sadiq S,Zhang W,Chen Y,Xu X,Abbas A,Chen S,Zhang R,Xue G,Sobotka D,Makinia J

    更新日期:2021-01-01 00:00:00

  • Effects of organic loading rates on the continuous electricity generation from fermented wastewater using a single-chamber microbial fuel cell.

    abstract::Novel coupling of single-chamber microbial fuel cells (MFCs) with granular activated carbon anodes were constructed, and their ability to produce electricity from fermented wastewater operating in continuous mode was investigated. MFCs treating real fermented wastewater can generate a power density of approximately 18...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.03.062

    authors: Nam JY,Kim HW,Lim KH,Shin HS

    更新日期:2010-01-01 00:00:00

  • Enhanced enzymatic saccharification of sugarcane bagasse pretreated by combining O2 and NaOH.

    abstract::Sugarcane bagasse pretreated by combining O2 and NaOH with different variables was conducted to improve its enzymatic digestibility and sugar recovery, and the results were compared with sole NaOH pretreatment. Lignin removal for O2-NaOH pretreatment was around 10% higher than that for sole NaOH pretreatment under the...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.05.041

    authors: Bi S,Peng L,Chen K,Zhu Z

    更新日期:2016-08-01 00:00:00

  • Dominant thermogravimetric signatures of lignin in cashew shell as compared to cashew shell cake.

    abstract::Dominant thermogravimetric signatures related to lignin were observed in cashew shell as compared to these signatures in cashew shell cake. The phenomenon of weakening of lignin from cashew shell to cashew shell cake was explained on the basis of changes in the activation energies. The pertinent temperature regimes re...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2013.12.087

    authors: Gangil S

    更新日期:2014-03-01 00:00:00

  • Degradation properties of protein and carbohydrate during sludge anaerobic digestion.

    abstract::Degradation of protein and carbohydrate is vital for sludge anaerobic digestion performance. However, few studies focused on degradation properties of protein and carbohydrate. This study investigated detailed degradation properties of sludge protein and carbohydrate in order to gain insight into organics removal duri...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.05.076

    authors: Yang G,Zhang P,Zhang G,Wang Y,Yang A

    更新日期:2015-09-01 00:00:00

  • Environmental sustainability of bioethanol produced from sweet sorghum stem on saline-alkali land.

    abstract::Life cycle assessment was conducted to evaluate the energy efficiency and environmental impacts of a bioethanol production system that uses sweet sorghum stem on saline-alkali land as feedstock. The system comprises a plant cultivation unit, a feedstock transport unit, and a bioethanol conversion unit, with 1000L of b...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.03.103

    authors: Wang M,Pan X,Xia X,Xi B,Wang L

    更新日期:2015-01-01 00:00:00

  • Utilization of bivalve shell-treated Zea mays L. (maize) husk leaf as a low-cost biosorbent for enhanced adsorption of malachite green.

    abstract::In this work, two low-cost wastes, bivalve shell (BS) and Zea mays L. husk leaf (ZHL), were investigated to adsorb malachite green (MG) from aqueous solutions. The ZHL was treated with calcined BS to give the BS-ZHL, and its ability to adsorb MG was compared with untreated ZHL, calcined BS and Ca(OH)(2)-treated ZHL un...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.06.066

    authors: Jalil AA,Triwahyono S,Yaakob MR,Azmi ZZ,Sapawe N,Kamarudin NH,Setiabudi HD,Jaafar NF,Sidik SM,Adam SH,Hameed BH

    更新日期:2012-09-01 00:00:00

  • Variation in bacterial community structure of aerobic granular and suspended activated sludge in the presence of the antibiotic sulfamethoxazole.

    abstract::The treatment performance and bacterial community structure of conventional activated sludge and aerobic granules exposed to antibiotic sulfamethoxazole (SMX) was studied. For three months, two sets of sequencing batch reactors inoculated with conventional and granular biomass were fed with a synthetic municipal waste...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2018.04.054

    authors: Kang AJ,Brown AK,Wong CS,Huang Z,Yuan Q

    更新日期:2018-08-01 00:00:00

  • In-situ injection of potassium hydroxide into briquetted wheat straw and meadow grass - Effect on biomethane production.

    abstract::Alkaline pretreatment of lignocellulosic biomass has been intensively investigated but heavy water usage and environmental pollution from wastewater limits its industrial application. This study presents a pretreatment technique by in-situ injection of potassium hydroxide concentrations ranging from 0.8% to 10% (w/w) ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2017.05.032

    authors: Feng L,Moset V,Li W,Chen C,Møller HB

    更新日期:2017-09-01 00:00:00

  • Influence of supports on catalytic behavior of nickel catalysts in carbon dioxide reforming of toluene as a model compound of tar from biomass gasification.

    abstract::A series of supported Ni catalysts including Ni/MgO, Ni/γ-Al2O3, Ni/α-Al2O3, Ni/SiO2 and Ni/ZrO2 was tested in CO2 reforming of toluene as a model compound of tar from biomass gasification in a fluidized bed reactor, and characterized by the means of temperature programmed reduction with hydrogen (H2-TPR), XRD, TEM an...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2010.09.054

    authors: Kong M,Fei J,Wang S,Lu W,Zheng X

    更新日期:2011-01-01 00:00:00

  • Gas controlled hydrogen fermentation.

    abstract::Acidogenic fermentation is an anaerobic process of double purpose, while treating organic residues it produces chemical compounds, such as hydrogen, ethanol and organic acids. Therefore, acidogenic fermentation arises as an attractive biotechnology process towards the biorefinery concept. Moreover, this process does n...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.01.122

    authors: Bastidas-Oyanedel JR,Mohd-Zaki Z,Zeng RJ,Bernet N,Pratt S,Steyer JP,Batstone DJ

    更新日期:2012-04-01 00:00:00

  • Recovery of humic-like susbtances from low quality composts.

    abstract::The quality of four commercial composts produced from poultry litter and municipal solid wastes was assessed based on their physic-chemical, stability and maturity parameters. These properties varied among the analysed composts; it was found that electric conductivity, heavy metals content and maturity were the parame...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2012.11.013

    authors: Silva ME,Lemos LT,Bastos MM,Nunes OC,Cunha-Queda AC

    更新日期:2013-01-01 00:00:00

  • Techno-economic and Monte Carlo probabilistic analysis of microalgae biofuel production system.

    abstract::This study focuses on the characterization of the technical and economic feasibility of an enclosed photobioreactor microalgae system with annual production of 37.85 million liters (10 million gallons) of biofuel. The analysis characterizes and breaks down the capital investment and operating costs and the production ...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.07.085

    authors: Batan LY,Graff GD,Bradley TH

    更新日期:2016-11-01 00:00:00

  • Robust enzymatic hydrolysis of Formiline-pretreated oil palm empty fruit bunches (EFB) for efficient conversion of polysaccharide to sugars and ethanol.

    abstract::Oil palm empty fruit bunch (EFB) was pretreated by Formiline process to overcome biomass recalcitrance and obtain hemicellulosic syrup and lignin. Higher formic acid concentration led to more lignin removal but also higher degree of cellulose formylation. Cellulose digestibility could be well recovered after deformyla...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2014.05.102

    authors: Cui X,Zhao X,Zeng J,Loh SK,Choo YM,Liu D

    更新日期:2014-08-01 00:00:00

  • Harvesting of microalgal biomass: Efficient method for flocculation through pH modulation.

    abstract::Harvesting of the micro alga Chlorococcum sp. R-AP13 through autoflocculation, chemical flocculants or by change in medium pH was evaluated. Surface charge of algal cells changed in response to the method used and affected flocculation efficiency. While aluminum sulfate and FeCl3 supported 87% and 92% efficiency, auto...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.03.114

    authors: Ummalyma SB,Mathew AK,Pandey A,Sukumaran RK

    更新日期:2016-08-01 00:00:00

  • Application of a horizontal subsurface flow constructed wetland on treatment of dairy parlor wastewater.

    abstract::Two horizontal subsurface flow reed beds of 75 m(2) each, treating dairy parlor effluent and domestic sewage (about 6.5 m(3)/day), were set-up to determine the efficiency of this system in reducing the polluting load in an isolated mountain rural settlement.A total suspended solids value of about 0.70 g/l and chemical...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/s0960-8524(02)00291-2

    authors: Mantovi P,Marmiroli M,Maestri E,Tagliavini S,Piccinini S,Marmiroli N

    更新日期:2003-06-01 00:00:00

  • Influence of microbial diversity and plant growth hormones in compost and vermicompost from fermented tannery waste.

    abstract::This study focuses on the effect of the epigeic earthworm Eudrilus eugeniae (with and without addition) to transform solid state fermented (SSF) and submerged (SmF) state fermented TFL mixed with cow dung and leaf litter into value added products in compost and vermicompost bioreactors respectively. The significant ro...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2016.03.032

    authors: Ravindran B,Wong JW,Selvam A,Sekaran G

    更新日期:2016-10-01 00:00:00

  • Heterologous expression of phosphoenolpyruvate carboxylase enhances the phosphate solubilizing ability of fluorescent pseudomonads by altering the glucose catabolism to improve biomass yield.

    abstract::The Synechococcus elongatus PCC 6301 phosphoenolpyruvate carboxylase (ppc) gene was constitutively overexpressed in fluorescent pseudomonads, to increase the supply of oxaloacetate, a crucial anabolic precursor and an intermediate in biosynthesis of organic acids implicated in phosphate (P) solubilization. Pseudomonas...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.08.075

    authors: Buch A,Archana G,Naresh Kumar G

    更新日期:2010-01-01 00:00:00

  • Effect of temperatures and pH on methyl violet biosorption by Mansonia wood sawdust.

    abstract::In this study, the effect of temperature on the equilibrium biosorption of methyl violet dye from aqueous solution using Mansonia wood sawdust was studied. The equilibrium biosorption data were analyzed using three widely applied isotherm models; Langmuir, Freundlich and Redlich-Peterson isotherm. The fit of three lin...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2007.11.018

    authors: Ofomaja AE,Ho YS

    更新日期:2008-09-01 00:00:00

  • Tolerance and removal of arsenic by a facultative marine fungus Aspergillus candidus.

    abstract::The objective of the work was to investigate tolerance to and removal of arsenic by a facultative marine fungus Aspergillus candidus. The fungus showed luxuriant growth in different concentrations (25 and 50mg/L) of trivalent and pentavalent forms of arsenic. Biomass accumulation data substantiate tolerance of A. cand...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2009.11.084

    authors: Vala AK

    更新日期:2010-04-01 00:00:00

  • Larvicidal activity of Tagetes patula essential oil against three mosquito species.

    abstract::Larvicidal activity of Tagetes patula essential oil was tested against the fourth instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefaciatus. Five different concentrations of essential oil were studied and the results were compared with that of synthetic insecticide, malathion. A. aegypti (LC(50) 1...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2004.10.020

    authors: Dharmagadda VS,Naik SN,Mittal PK,Vasudevan P

    更新日期:2005-07-01 00:00:00

  • Carbon recovery by fermentation of CO-rich off gases - Turning steel mills into biorefineries.

    abstract::Technological solutions to reduce greenhouse gas (GHG) emissions from anthropogenic sources are required. Heavy industrial processes, such as steel making, contribute considerably to GHG emissions. Fermentation of carbon monoxide (CO)-rich off gases with wild-type acetogenic bacteria can be used to produce ethanol, ac...

    journal_title:Bioresource technology

    pub_type: 杂志文章,评审

    doi:10.1016/j.biortech.2016.03.094

    authors: Molitor B,Richter H,Martin ME,Jensen RO,Juminaga A,Mihalcea C,Angenent LT

    更新日期:2016-09-01 00:00:00

  • Characterization of the anaerobic denitrification bacterium Acinetobacter sp. SZ28 and its application for groundwater treatment.

    abstract::Acinetobacter sp. SZ28 exhibited efficient autotrophic denitrification ability using Mn(2+) as an electron donor. Sequence amplification identified the presence of the nirS gene. Meteorological chromatography analysis showed that N2 was produced as an end product. Response surface methodology experiments showed that t...

    journal_title:Bioresource technology

    pub_type: 杂志文章

    doi:10.1016/j.biortech.2015.06.020

    authors: Su Jf,Zheng SC,Huang Tl,Ma F,Shao SC,Yang SF,Zhang Ln

    更新日期:2015-09-01 00:00:00