Solvent and Water Mediated Structural Variations in Deoxynivalenol and Their Potential Implications on the Disruption of Ribosomal Function.

Abstract:

:Fusarium head blight (FHB) is a disease of cereal crops caused by trichothecene producing Fusarium species. Trichothecenes, macrocylicic fungal metabolites composed of three fused rings (A-C) with one epoxide functionality, are a class of mycotoxins known to inhibit protein synthesis in eukaryotic ribosomes. These toxins accumulate in the kernels of infected plants rendering them unsuitable for human and animal consumption. Among the four classes of trichothecenes (A-D) A and B are associated with FHB, where the type B trichothecene deoxynivalenol (DON) is most relevant. While it is known that these toxins inhibit protein synthesis by disrupting peptidyl transferase activity, the exact mechanism of this inhibition is poorly understood. The three-dimensional structures and H-bonding behavior of DON were evaluated using one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy techniques. Comparisons of the NMR structure presented here with the recently reported crystal structure of DON bound in the yeast ribosome reveal insights into the possible toxicity mechanism of this compound. The work described herein identifies a water binding pocket in the core structure of DON, where the 3OH plays an important role in this interaction. These results provide preliminary insights into how substitution at C3 reduces trichothecene toxicity. Further investigations along these lines will provide opportunities to develop trichothecene remediation strategies based on the disruption of water binding interactions with 3OH.

journal_name

Front Microbiol

authors

Foroud NA,Shank RA,Kiss D,Eudes F,Hazendonk P

doi

10.3389/fmicb.2016.01239

subject

Has Abstract

pub_date

2016-08-17 00:00:00

pages

1239

issn

1664-302X

journal_volume

7

pub_type

杂志文章
  • Urea Amendment Decreases Microbial Diversity and Selects for Specific Nitrifying Strains in Eight Contrasting Agricultural Soils.

    abstract::Application of nitrogen (N) fertilizers, predominantly as urea, is a major source of reactive N in the environment, with wide ranging effects including increased greenhouse gas accumulation in the atmosphere and aquatic eutrophication. The soil microbial community is the principal driver of soil N cycling; thus, impro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00634

    authors: Staley C,Breuillin-Sessoms F,Wang P,Kaiser T,Venterea RT,Sadowsky MJ

    更新日期:2018-04-04 00:00:00

  • A Pilin Region Affecting Host Range of the Pseudomonas aeruginosa RNA Phage, PP7.

    abstract::The host range of a phage is determined primarily by phage-receptor interaction. Here, we profiled the host range of an RNA leviphage, PP7 that requires functional type IV pilus (TFP) in order to enter into its host bacterium, Pseudomonas aeruginosa. Out of 25 twitching-proficient P. aeruginosa strains, 4 with group I...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00247

    authors: Kim ES,Bae HW,Cho YH

    更新日期:2018-02-16 00:00:00

  • Efficient Biofilm-Based Fermentation Strategies for L-Threonine Production by Escherichia coli.

    abstract::Biofilms provide cells favorable growth conditions, which have been exploited in industrial biotechnological processes. However, industrial application of the biofilm has not yet been reported in Escherichia coli, one of the most important platform strains, though the biofilm has been extensively studied for pathogeni...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01773

    authors: Chen T,Liu N,Ren P,Xi X,Yang L,Sun W,Yu B,Ying H,Ouyang P,Liu D,Chen Y

    更新日期:2019-08-02 00:00:00

  • Sterol Synthesis in Diverse Bacteria.

    abstract::Sterols are essential components of eukaryotic cells whose biosynthesis and function has been studied extensively. Sterols are also recognized as the diagenetic precursors of steranes preserved in sedimentary rocks where they can function as geological proxies for eukaryotic organisms and/or aerobic metabolisms and en...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00990

    authors: Wei JH,Yin X,Welander PV

    更新日期:2016-06-24 00:00:00

  • Programming Bacteria With Light-Sensors and Applications in Synthetic Biology.

    abstract::Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices in eukaryotic cells have been relatively established, trials in bacterial cells have only been emerging in the past few years. A number of light sensors have ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02692

    authors: Liu Z,Zhang J,Jin J,Geng Z,Qi Q,Liang Q

    更新日期:2018-11-08 00:00:00

  • Staphylococcus aureus Isolates from Goat and Sheep Milk Seem to Be Closely Related and Differ from Isolates Detected from Bovine Milk.

    abstract::Dairy goat and sheep farms suffer severe economic losses due to intramammary infections, with Staphylococcus aureus representing the main cause of clinical mastitis in small ruminants. In addition, S. aureus contamination of goat and sheep milk may cause staphylococcal food poisoning, as many traditional caprine and o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00319

    authors: Merz A,Stephan R,Johler S

    更新日期:2016-03-14 00:00:00

  • Changes in the Fungal Microbiome of Maize During Hermetic Storage in the United States and Kenya.

    abstract::Prior to harvest, maize kernels are invaded by a diverse population of fungal organisms that comprise the microbiome of the grain mass. Poor post-harvest practices and improper drying can lead to the growth of mycotoxigenic storage fungi and deterioration of grain quality. Hermetic storage bags are a low-cost technolo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02336

    authors: Lane B,Sharma S,Niu C,Maina AW,Wagacha JM,Bluhm BH,Woloshuk CP

    更新日期:2018-10-02 00:00:00

  • Peeling the Layers Away: The Genomic Characterization of Bacillus pumilus 64-1, an Isolate With Antimicrobial Activity From the Marine Sponge Plakina cyanorosea (Porifera, Homoscleromorpha).

    abstract::Bacillus pumilus 64-1, a bacterial strain isolated from the marine sponge Plakina cyanorosea, which exhibits antimicrobial activity against both pathogenic and drug-resistant Gram-positive and Gram-negative bacteria. This study aimed to conduct an in-depth genomic analysis of this bioactive sponge-derived strain. The ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592735

    authors: Freitas-Silva J,de Oliveira BFR,Vigoder FM,Muricy G,Dobson ADW,Laport MS

    更新日期:2021-01-08 00:00:00

  • Subversion of mucosal barrier polarity by pseudomonas aeruginosa.

    abstract::The lumenal surfaces of human body are lined by a monolayer of epithelia that together with mucus secreting cells and specialized immune cells form the mucosal barrier. This barrier is one of the most fundamental components of the innate immune system, protecting organisms from the vast environmental microbiota. The m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2011.00114

    authors: Engel J,Eran Y

    更新日期:2011-05-26 00:00:00

  • The GC Content as a Main Factor Shaping the Amino Acid Usage During Bacterial Evolution Process.

    abstract::Understanding how proteins evolve is important, and the order of amino acids being recruited into the genetic codons was found to be an important factor shaping the amino acid composition of proteins. The latest work about the last universal common ancestor (LUCA) makes it possible to determine the potential factors s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02948

    authors: Du MZ,Zhang C,Wang H,Liu S,Wei W,Guo FB

    更新日期:2018-12-07 00:00:00

  • Strong Inference in Mathematical Modeling: A Method for Robust Science in the Twenty-First Century.

    abstract::While there are many opinions on what mathematical modeling in biology is, in essence, modeling is a mathematical tool, like a microscope, which allows consequences to logically follow from a set of assumptions. Only when this tool is applied appropriately, as microscope is used to look at small items, it may allow to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01131

    authors: Ganusov VV

    更新日期:2016-07-22 00:00:00

  • Gut Microbial Dysbiosis and Plasma Metabolic Profile in Individuals With Vitiligo.

    abstract::Autoimmune diseases are increasingly linked to aberrant gut microbiome and relevant metabolites. However, the association between vitiligo and the gut microbiome remains to be elucidated. Thus, we conducted a case-control study through 16S rRNA sequencing and serum untargeted-metabolomic profiling based on 30 vitiligo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.592248

    authors: Ni Q,Ye Z,Wang Y,Chen J,Zhang W,Ma C,Li K,Liu Y,Liu L,Han Z,Gao T,Jian Z,Li S,Li C

    更新日期:2020-12-14 00:00:00

  • Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria.

    abstract::Microorganisms possess enormous highly specific metabolic activities, which enable them to utilize and transform nearly every known chemical class present in crude oil. In this context, one of the most studied biocatalytic processes is the biodesulfurization (BDS) of thiophenic sulfur-containing compounds such as benz...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00112

    authors: Mohamed Mel-S,Al-Yacoub ZH,Vedakumar JV

    更新日期:2015-02-13 00:00:00

  • Variability of Gut Microbiota Across the Life Cycle of Grapholita molesta (Lepidoptera: Tortricidae).

    abstract::Grapholita molesta, the oriental fruit moth, is a serious global pest of many Rosaceae fruit trees. Gut microorganisms play important roles in host nutrition, digestion, detoxification, and resistance to pathogens. However, there are few studies on the microbiota of G. molesta, particularly during metamorphosis. Here,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01366

    authors: Wang X,Sun S,Yang X,Cheng J,Wei H,Li Z,Michaud JP,Liu X

    更新日期:2020-06-30 00:00:00

  • Morpholino-Mediated Knockdown of Ciliary Genes in Euplotes vannus, a Novel Marine Ciliated Model Organism.

    abstract::Cilia are highly conserved organelles present in almost all types of eukaryotic cells, and defects in cilia structure and/or function are related to many human genetic disorders. Single-celled ciliated protists, which possess diverse types of cilia, are remarkable model organisms for studying cilia structures and func...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.549781

    authors: Tang D,Wang X,Dong J,Li Y,Gao F,Xie H,Zhao C

    更新日期:2020-10-19 00:00:00

  • Post-translational Modification-Based Regulation of HIV Replication.

    abstract::Human immunodeficiency virus (HIV) relies heavily on the host cellular machinery for production of viral progeny. To exploit cellular proteins for replication and to overcome host factors with antiviral activity, HIV has evolved a set of regulatory and accessory proteins to shape an optimized environment for its repli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.02131

    authors: Chen L,Keppler OT,Schölz C

    更新日期:2018-09-11 00:00:00

  • Complementary DNA/RNA-Based Profiling: Characterization of Corrosive Microbial Communities and Their Functional Profiles in an Oil Production Facility.

    abstract::DNA and RNA-based sequencing of the 16S rRNA gene and transcripts were used to assess the phylogenetic diversity of microbial communities at assets experiencing corrosion in an oil production facility. The complementary methodological approach, coupled with extensive bioinformatics analysis, allowed to visualize diffe...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02587

    authors: Salgar-Chaparro SJ,Machuca LL

    更新日期:2019-11-07 00:00:00

  • The Compromised Mucosal Immune System of β7 Integrin-Deficient Mice Has Only Minor Effects on the Fecal Microbiota in Homeostasis.

    abstract::The gastrointestinal tract is an ideal habitat for diverse bacterial species that reside in a homeostatic balance with local tissue and significantly contribute to host health. Negative shifts in gut microbiota profiles, also known as dysbiosis, may be implicated in the development of chronic disorders such as inflamm...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02284

    authors: Babbar A,Hitch TCA,Pabst O,Clavel T,Hübel J,Eswaran S,Wagner N,Schippers A

    更新日期:2019-10-04 00:00:00

  • Prioritized Expression of BDH2 under Bulk Translational Repression and Its Contribution to Tolerance to Severe Vanillin Stress in Saccharomyces cerevisiae.

    abstract::Vanillin is a potent fermentation inhibitor derived from the lignocellulosic biomass in biofuel production, and high concentrations of vanillin result in the pronounced repression of bulk translation in Saccharomyces cerevisiae. Studies on genes that are efficiently translated even in the presence of high concentratio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01059

    authors: Ishida Y,Nguyen TT,Kitajima S,Izawa S

    更新日期:2016-07-06 00:00:00

  • Responses of beneficial Bacillus amyloliquefaciens SQR9 to different soilborne fungal pathogens through the alteration of antifungal compounds production.

    abstract::Bacillus amyloliquefaciens SQR9 exhibited predominantly antagonistic activities against a broad range of soilborne pathogens. The fungi-induced SQR9 extracts possess stronger antifungal activities compared with SQR9 monoculture extracts. To investigate how SQR9 fine-tunes lipopeptides (LPs) and a siderophore bacilliba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00636

    authors: Li B,Li Q,Xu Z,Zhang N,Shen Q,Zhang R

    更新日期:2014-11-21 00:00:00

  • Cellular Responses of the Lichen Circinaria gyrosa in Mars-Like Conditions.

    abstract::Lichens are extremely resistant organisms that colonize harsh climatic areas, some of them defined as "Mars-analog sites." There still remain many unsolved questions as to how lichens survive under such extreme conditions. Several studies have been performed to test the resistance of various lichen species under space...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00308

    authors: de la Torre Noetzel R,Miller AZ,de la Rosa JM,Pacelli C,Onofri S,García Sancho L,Cubero B,Lorek A,Wolter D,de Vera JP

    更新日期:2018-03-05 00:00:00

  • Beyond 16S rRNA Community Profiling: Intra-Species Diversity in the Gut Microbiota.

    abstract::Interactions with microbes affect many aspects of animal biology, including immune system development, nutrition and health. In vertebrates, the gut microbiota is dominated by a small subset of phyla, but the species composition within these phyla is typically not conserved. Moreover, several recent studies have shown...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01475

    authors: Ellegaard KM,Engel P

    更新日期:2016-09-21 00:00:00

  • Trends and future challenges in sampling the deep terrestrial biosphere.

    abstract::Research in the deep terrestrial biosphere is driven by interest in novel biodiversity and metabolisms, biogeochemical cycling, and the impact of human activities on this ecosystem. As this interest continues to grow, it is important to ensure that when subsurface investigations are proposed, materials recovered from ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00481

    authors: Wilkins MJ,Daly RA,Mouser PJ,Trexler R,Sharma S,Cole DR,Wrighton KC,Biddle JF,Denis EH,Fredrickson JK,Kieft TL,Onstott TC,Peterson L,Pfiffner SM,Phelps TJ,Schrenk MO

    更新日期:2014-09-12 00:00:00

  • Negative Plant-Soil Feedback Driven by Re-assemblage of the Rhizosphere Microbiome With the Growth of Panax notoginseng.

    abstract::There is a concerted understanding of the accumulation of soil pathogens as the major driving factor of negative plant-soil feedback (NPSF). However, our knowledge of the connection between plant growth, pathogen build-up and soil microbiome assemblage is limited. In this study, significant negative feedback between t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01597

    authors: Luo L,Guo C,Wang L,Zhang J,Deng L,Luo K,Huang H,Liu Y,Mei X,Zhu S,Yang M

    更新日期:2019-07-26 00:00:00

  • Biotechnology and pasta-making: lactic Acid bacteria as a new driver of innovation.

    abstract::Cereals-derived foods represent a key constituent in the diet of many populations. In particular, pasta is consumed in large quantities throughout the world in reason of its nutritive importance, containing significant amounts of complex carbohydrates, proteins, B-vitamins, and iron. Lactic acid bacteria (LAB) are a h...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00094

    authors: Capozzi V,Russo P,Fragasso M,De Vita P,Fiocco D,Spano G

    更新日期:2012-03-15 00:00:00

  • Diet-Induced Obesity Does Not Alter Tigecycline Treatment Efficacy in Murine Lyme Disease.

    abstract::Obese individuals more frequently suffer from infections, as a result of increased susceptibility to a number of bacterial pathogens. Furthermore, obesity can alter antibiotic treatment efficacy due to changes in drug pharmacokinetics which can result in under-dosing. However, studies on the treatment of bacterial inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00292

    authors: Pětrošová H,Eshghi A,Anjum Z,Zlotnikov N,Cameron CE,Moriarty TJ

    更新日期:2017-02-24 00:00:00

  • The Complex Essential Oils Highly Control the Toxigenic Fungal Microbiome and Major Mycotoxins During Storage of Maize.

    abstract::The contamination of maize with fungi and subsequent mycotoxins is a pivotal and long-standing safety concern in the maize industry. In this study, the inhibitory effects of the complex essential oils (cinnamaldehyde, citral, eugenol, and menthol, 3:3:2:2, v/v) on fungal growth and mycotoxins production in stored maiz...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01643

    authors: Wang L,Liu B,Jin J,Ma L,Dai X,Pan L,Liu Y,Zhao Y,Xing F

    更新日期:2019-07-16 00:00:00

  • Reduced vancomycin susceptibility in porcine ST9 MRSA isolates.

    abstract::Porcine strains of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) have been recognized in many countries and have been shown to be able to cause human infection. Resistance to non-beta lactam antibiotics has been reported but non-susceptibility to vancomycin, which is known to occur in huma...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2013.00316

    authors: Kwok GM,O'Donoghue MM,Doddangoudar VC,Ho J,Boost MV

    更新日期:2013-10-25 00:00:00

  • Increased Denitrification Rates Associated with Shifts in Prokaryotic Community Composition Caused by Varying Hydrologic Connectivity.

    abstract::While modern developments in agriculture have allowed for increases in crop yields and rapid human population growth, they have also drastically altered biogeochemical cycles, including the biotransformation of nitrogen. Denitrification is a critical process performed by bacteria and fungi that removes nitrate in surf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02304

    authors: Tomasek A,Staley C,Wang P,Kaiser T,Lurndahl N,Kozarek JL,Hondzo M,Sadowsky MJ

    更新日期:2017-11-22 00:00:00

  • Characterization and Metabolism Effect of Seed Endophytic Bacteria Associated With Peanut Grown in South China.

    abstract::Endophytes are considered to be excellent biocontrol agents and biofertilizers, and are associated with plant growth promotion and health. In particular, seed-endophytic bacteria benefit the host plant's progeny via vertical transmission, and can play a role in plant growth and defense. However, seed-associated endoph...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.02659

    authors: Li L,Zhang Z,Pan S,Li L,Li X

    更新日期:2019-11-13 00:00:00