当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL IMMUNOLOGY期刊下所有文献 > Evaluation of the safety and adjuvant effect of a detoxified listeriolysin O mutant on the humoral response to dengue virus antigens.

Evaluation of the safety and adjuvant effect of a detoxified listeriolysin O mutant on the humoral response to dengue virus antigens.

Abstract：

:Listeriolysin O (LLO) has been proposed as a potential carrier or adjuvant molecule in the vaccination field. However, the cytotoxic and pro-apoptotic effects of LLO are the major limitations for this purpose. Here, we have performed a preclinical safety evaluation and characterized a new potential adjuvant application for a non-cytolytic LLO mutant (dtLLO) to enhance and modulate the immune response against the envelope (E) protein from dengue virus. In addition, we have studied the adjuvant effects of dtLLO on human immune cells and the role of membrane cholesterol for the binding and proinflammatory property of the toxoid. Our in-vivo results in the murine model confirmed that dtLLO is a safer molecule than wild-type LLO (wtLLO), with a significantly increased survival rate for mice challenged with dtLLO compared with mice challenged with wtLLO (P < 0·001). Histopathological analysis showed non-toxic effects in key target organs such as brain, heart, liver, spleen, kidney and lung after challenge with dtLLO. In vitro, dtLLO retained the capacity of binding to plasma membrane cholesterol on the surface of murine and human immune cells. Immunization of 6-8-week-old female BALB/c mice with a combination of dtLLO mixed with E protein elicited a robust specific humoral response with isotype diversification of immunoglobulin (Ig)G antibodies (IgG1 and IgG2a). Finally, we demonstrated that cholesterol and lipid raft integrity are required to induce a proinflammatory response by human cells. Taken together, these findings support a potential use of the dtLLO mutant as a safe and effective adjuvant molecule in vaccination.

journal_name

Clin Exp Immunol

journal_title

Clinical and experimental immunology

authors

Hernández-Flores KG,Calderón-Garcidueñas AL,Mellado-Sánchez G,Ruiz-Ramos R,Sánchez-Vargas LA,Thomas-Dupont P,Izaguirre-Hernández IY,Téllez-Sosa J,Martínez-Barnetche J,Wood L,Paterson Y,Cedillo-Barrón L,López-Franco O,Vivanco-Ci

doi

10.1111/cei.12906

subject

Has Abstract

pub_date

2017-04-01 00:00:00

pages

109-126

issue

1

eissn

0009-9104

issn

1365-2249

journal_volume

188

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL IMMUNOLOGY文献大全
  • Multiple sclerosis: II. Effects of prothymosin alpha on the autologous and allogeneic MLR in patients with multiple sclerosis.

    abstract：:We have recently demonstrated that peripheral blood monocytes from patients with multiple sclerosis (MS) have a defect in stimulating autologous and allogeneic T lymphocytes. This defect was found to correlate with disease activity. In this report we demonstrate that prothymosin alpha (ProT alpha), a rat thymus fracti...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Reclos GJ,Baxevanis CN,Sfagos C,Papageorgiou C,Tsokos GC,Papamichail M

    更新日期：1987-11-01 00:00:00

  • Immunology of membranous nephropathy: from animal models to humans.

    abstract：:Membranous nephropathy (MN), the leading cause of nephrotic syndrome in adults, is characterized by the deposition of subepithelial immune deposits that consist mainly of immunoglobulin (Ig)G and complement. Most of the cases are primary or idiopathic (iMN), while only approximately 25% of the cases are secondary to s...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.12729

    authors： Sinico RA,Mezzina N,Trezzi B,Ghiggeri GM,Radice A

    更新日期：2016-02-01 00:00:00

  • Delayed disease progression in HIV-2: the importance of TRIM5α and the retroviral capsid.

    abstract：:HIV-2 is thought to have entered the human population in the 1930s through cross-species transmission of SIV from sooty mangabeys in West Africa. Unlike HIV-1, HIV-2 has not led to a global pandemic, and recent data suggest that HIV-2 prevalence is declining in some West African states where it was formerly endemic. A...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/cei.13280

    authors： Boswell MT,Rowland-Jones SL

    更新日期：2019-06-01 00:00:00

  • NK cells, displaying early activation, cytotoxicity and adhesion molecules, are associated with mild dengue disease.

    abstract：:During the innate immune response against infections, Natural Killer (NK) cells are as important effector cells as are Cytotoxic T lymphocytes (CTL) generated after antigenic stimulation in the adaptative response. NK cells increase in numbers, after viral infection or vaccination. We investigated the NK cell and CD8 ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.02996.x

    authors： Azeredo EL,De Oliveira-Pinto LM,Zagne SM,Cerqueira DI,Nogueira RM,Kubelka CF

    更新日期：2006-02-01 00:00:00

  • Clinical significance of immunopathological findings in patients with post-pericardiotomy syndrome. II. The significance of serum inhibition and rosette inhibitory factors.

    abstract：:Serum inhibition factors (SIF) that suppress phytohaemagglutinin-induced blast transformation of normal lymphocytes, and lymphocyte E-rosette inhibitory factors (RIF) that inhibit the T cell-specific property of E-rosette formation were determined in sixty-five patients before and after cardiac surgery. SIF was found ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Maisch B,Schuff-Werner P,Berg PA,Kochsiek K

    更新日期：1979-11-01 00:00:00

  • The effects of purified mitogenic proteins (Pa-1 and Pa-2) from pokeweed on human T and B lymphocytes in vitro.

    abstract：:Purified proteins (Pa-1 and Pa-2) from pokeweed have been compared with commercial pokeweed mitogen (PWM-G) and other mitogens in their ability to stimulate human lymphocytes. With cultures of T and B cells separated from tonsil lymphocytes, thymidine uptake, blast transformation and immunoglobulin (Ig) synthesis have...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Janossy G,Gomez De La Concha E,Waxdal MJ,Platts-Mills T

    更新日期：1976-10-01 00:00:00

  • Monocyte-mediated suppression of anti-DNA antibody production in healthy subjects.

    abstract：:Monocyte-depleted mononuclear cells (MNC) from healthy subjects synthesized IgM-class antibody to single-stranded DNA (anti-ssDNA) at significantly higher levels than unfractionated MNC. The increased production of IgM anti-ssDNA by monocyte-depleted normal MNC was inhibited to a greater degree than total IgM by addit...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Okawa-Takatsuji M,Aotsuka S,Uwatoko S,Kinoshita M,Yokohari R

    更新日期：1989-09-01 00:00:00

  • Purification of normal human eosinophils using the different binding capacities of blood leucocytes for complexed rabbit IgG.

    abstract：:A method has been developed for separating eosinophils from other types of leucocyte in normal individuals. Erythrocytes are sedimented with dextran, and mononuclear cells are removed on an isotonic density gradient of ficoll and sodium diatrizoate. The eosinophils and neutrophils are then washed and sedimented onto p...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Tai PC,Spry CJ

    更新日期：1977-05-01 00:00:00

  • Haptenic antibody induced by N-phthaloylated ox insulin in the Hartley guinea-pig.

    abstract：:Female Hartley guinea-pigs were immunized with N-phthaloylated ox insulin (96% NA1,NB1,NB29-triphthaloyl ox insulin) using H. pertussis as adjuvant. Haptenic antibody, which was found in antisera taken 20 days after secondary immunization, was assessed for cross-reaction with a series of N-acylated ox insulins. Cross-...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Hollins PJ,Nathan MM

    更新日期：1978-03-01 00:00:00

  • Effect of non-operative management (NOM) of splenic rupture versus splenectomy on the distribution of peripheral blood lymphocyte populations and cytokine production by T cells.

    abstract：:Post-traumatic splenectomy is associated with increased postoperative morbidity and mortality and long-term impairment of humoral and cellular immunity. Alternatives to surgery have been developed to minimize or avoid the immediate and/or long-term complications of splenectomy. Herein we investigated the long-term eff...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2007.03517.x

    authors： Theodorou GL,Mouzaki A,Tsiftsis D,Apostolopoulou A,Mougiou A,Theodori E,Vagianos C,Karakantza M

    更新日期：2007-12-01 00:00:00

  • Immune complexome analysis reveals the presence of immune complexes and identifies disease-specific immune complex antigens in saliva samples from patients with Sjögren's syndrome.

    abstract：:Sjögren's syndrome (SS) is a chronic autoimmune disease that mainly damages the salivary and lacrimal glands. Immune complex (IC) formation triggers local inflammation through IC deposition and decreased antigen function. Some ICs can leak from the lesion and into the saliva but no salivary ICs have been reported to d...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.13574

    authors： Yamane K,Nakamura H,Hamasaki M,Minei Y,Aibara N,Shimizu T,Kawakami A,Nakashima M,Kuroda N,Ohyama K

    更新日期：2021-01-12 00:00:00

  • Relationships between humoral factors in HIV-1-infected mothers and the occurrence of HIV infection in their infants.

    abstract：:Based on what is known about the biology of HIV-1 vertical transmission, the HIV burden of the mother, maternal immune factors and the integrity of the placental barrier are likely to play major roles. We therefore sought to determine whether the presence of antibodies in sera from 47 HIV-1-infected mothers, including...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1995.tb03840.x

    authors： Mabondzo A,Rouvier P,Raoul H,Le Naour R,Courpotin C,Hervé F,Parnet-Mathieu F,Lasfargues G,Dormont D

    更新日期：1995-12-01 00:00:00

  • Expression of high-affinity IL-4 receptors on human melanoma, ovarian and breast carcinoma cells.

    abstract：:It has previously been shown that murine sarcoma cells express high-affinity IL-4 receptors (IL-4R) which are internalized after binding to the ligand (Puri et al., Cancer Res 1991; 51:3011-7). We have also reported that human renal cell carcinoma cells express high-affinity IL-4R, and IL-4 inhibits tumour growth in v...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.1994.tb06029.x

    authors： Obiri NI,Siegel JP,Varricchio F,Puri RK

    更新日期：1994-01-01 00:00:00

  • Heymann nephritis in rats induced by human renal tubular antigens: characterization of antigen and antibody specificities.

    abstract：:An injection of human renal tubular fraction, Fx1A, with Freund's complete adjuvant in rats induces Heymann nephritis (HN) with clinical and morphological features similar to rat Fx1A-induced HN. The present study was undertaken to characterize antibody specificities and the corresponding antigen involved in human Fx1...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Natori Y,Hayakawa I,Shibata S

    更新日期：1987-07-01 00:00:00

  • Membrane-bound interleukin-21 and CD137 ligand induce functional human natural killer cells from peripheral blood mononuclear cells through STAT-3 activation.

    abstract：:Natural killer (NK) cell adoptive transfer is a promising approach for cancer immunotherapy; however, its development has been hindered by the lack of efficient methods to produce large numbers of functional NK cells. In this study, we engineered the leukaemia cell line K562 to express CD137 ligand (CD137L) and membra...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12034

    authors： Wang X,Lee DA,Wang Y,Wang L,Yao Y,Lin Z,Cheng J,Zhu S

    更新日期：2013-04-01 00:00:00

  • Surface-reactive antibodies to human adrenal cells in Addison's disease.

    abstract：:Organ-specific surface-reactive antibodies to viable human adrenal cell suspensions from adult or fetal glands were detected by indirect immunofluorescence (IFL) in 24 out of 28 idiopathic Addison's disease sera with adrenal cytoplasmic antibodies. Cell-surface reactions were also present in nine out of 10 cases of po...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Khoury EL,Hammond L,Bottazzo GF,Doniach D

    更新日期：1981-07-01 00:00:00

  • Characterization and demonstration of human liver-specific protein (LSP) and apo-LSP.

    abstract：:Liver-specific protein (LSP) prepared by standard methods from five normal human livers showed significant variations in terms of quantitative yield, lipid/protein ratio and migration characteristics of different components on SDS-polyacrylamide gel electrophoresis. This heterogeneity is probably related to varying am...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Lebwohl N,Gerber MA

    更新日期：1981-11-01 00:00:00

  • Blastogenic responses to Pneumocystis carinii among patients with human immunodeficiency (HIV) infection.

    abstract：:Proliferative responses by human peripheral blood mononuclear cells and rat spleen cells were measured to Pneumocystis carinii in a blastogenic assay using organisms obtained from rat lungs and propagated in tissue culture as the antigen. Responses occurred only in subjects with known prior exposure to P. carinii and ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Hagler DN,Deepe GS,Pogue CL,Walzer PD

    更新日期：1988-10-01 00:00:00

  • Macrophage activation switching: an asset for the resolution of inflammation.

    abstract：:Macrophages play a central role in inflammation and host defence against microorganisms, but they also participate actively in the resolution of inflammation after alternative activation. However, it is not known whether the resolution of inflammation requires alternative activation of new resting monocytes/macrophage...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02934.x

    authors： Porcheray F,Viaud S,Rimaniol AC,Léone C,Samah B,Dereuddre-Bosquet N,Dormont D,Gras G

    更新日期：2005-12-01 00:00:00

  • Correlations between serum factor B and C3b inactivator levels in normal subjects and in patients with infections, nephrosis and hypocomplementaemic glomerulonephritis.

    abstract：:In normal subjects, factor B and C3b inactivator (KAF) levels were linearly related (r = 0-71); factor B levels in subjects with low normal levels of KAF were significantly lower than in those with high normal levels of KAF. This relationship is postulated to be the result of modulation by the level of KAF of the avai...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Forristal J,Iitaka K,Vallota EH,West CD

    更新日期：1977-04-01 00:00:00

  • The distribution of a specific HLA-B27-associated cell surface component on the tissues of patients with ankylosing spondylitis.

    abstract：:In this paper, we report the presence on Epstein-Barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an HLA-B27-associated cell surface complex (antigenically related to some antigens of Klebsiella K43 and K21) which is identical to or cross-reactive with the determinant present on the ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Alexander K,Edwards C,Misko IS,Geczy AF,Bashir HV,Edmonds JP

    更新日期：1981-07-01 00:00:00

  • Onchocerca volvulus-specific antibody and cytokine responses in onchocerciasis patients after 16 years of repeated ivermectin therapy.

    abstract：:The recommended control option against onchocerciasis is repeated ivermectin treatment, which will need to be implemented for decades, and it remains unknown how repeated ivermectin therapy might affect immunity against Onchocerca volvulus in the long term. O. volvulus-specific antibody reactivity and cellular cytokin...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2006.03312.x

    authors： Mai CS,Hamm DM,Banla M,Agossou A,Schulz-Key H,Heuschkel C,Soboslay PT

    更新日期：2007-03-01 00:00:00

  • Immunosuppression by seminal prostaglandins.

    abstract：:In this paper we report studies undertaken to determine the contribution of seminal prostaglandins to some of the known immunosuppressive properties of human seminal plasma. Initial studies revealed that fractions of seminal plasma enriched in E series prostaglandins, obtained by reverse phase chromatography, had a pr...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Quayle AJ,Kelly RW,Hargreave TB,James K

    更新日期：1989-03-01 00:00:00

  • Blunted expression of miR-199a-5p in regulatory T cells of patients with chronic obstructive pulmonary disease compared to unaffected smokers.

    abstract：:Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal regulatory T cell (T(reg)) response and increases in T helper type 1 (Th1) and Th17 cell responses. It is unclear if dysregulation of microRNAs (miRNA) within T(reg) cells contributes to the abnormal inflammatory response in COPD. In this stu...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12325

    authors： Chatila WM,Criner GJ,Hancock WW,Akimova T,Moldover B,Chang JK,Cornwell W,Santerre M,Rogers TJ

    更新日期：2014-07-01 00:00:00

  • Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses: position statement of the T-Cell Workshop Committee of the Immunology of Diabetes Society.

    abstract：:Autoimmune T cell responses directed against insulin-producing β cells are central to the pathogenesis of type 1 diabetes (T1D). Detection of such responses is therefore critical to provide novel biomarkers for T1D 'immune staging' and to understand the mechanisms underlying the disease. While different T cell assays ...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2249.2010.04272.x

    authors： Mallone R,Mannering SI,Brooks-Worrell BM,Durinovic-Belló I,Cilio CM,Wong FS,Schloot NC,T-Cell Workshop Committee, Immunology of Diabetes Society.

    更新日期：2011-01-01 00:00:00

  • Cellular immune responses against hepatitis C virus: the evidence base 2002.

    abstract：:Hepatitis C virus (HCV) is an RNA virus which is estimated to persistently infect about 170 million people worldwide. After acute infection, there is an initial period during which long-term outcome is decided. There is strong evidence that the cellular immune responses, involving both CD4+ and CD8+ T lymphocytes, are...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-2249.2002.01840.x

    authors： Ward S,Lauer G,Isba R,Walker B,Klenerman P

    更新日期：2002-05-01 00:00:00

  • Systemic inflammation in acute intermittent porphyria: a case-control study.

    abstract：:This study aimed to examine whether acute intermittent porphyria (AIP) is associated with systemic inflammation and whether the inflammation correlates with disease activity. A case-control study with 50 AIP cases and age-, sex- and place of residence-matched controls was performed. Plasma cytokines, insulin and C-pep...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/cei.12899

    authors： Storjord E,Dahl JA,Landsem A,Fure H,Ludviksen JK,Goldbeck-Wood S,Karlsen BO,Berg KS,Mollnes TE,W Nielsen E,Brekke OL

    更新日期：2017-03-01 00:00:00

  • Suppression of natural and activated human antitumour cytotoxicity by human seminal plasma.

    abstract：:The influence of human seminal plasma (SP) and whole semen (S) on the expression of natural cytotoxicity by human peripheral blood mononuclear cells (PBMNC) was examined. Marked suppression of natural cytotoxicity against K562 targets was observed when effectors were pre-treated for 1 h with SP or S diluted up to 1:40...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：

    authors： Rees RC,Vallely P,Clegg A,Potter CW

    更新日期：1986-03-01 00:00:00

  • T cell clones from a Sjögren's syndrome salivary gland biopsy produce high levels of IL-10.

    abstract：:Sjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory CD4+ Th1-like phenotype and express high levels of class II, though CD8+ cells are also present. We have studied 17 labial salivary gland and 15 periphe...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2249.1996.d01-623.x

    authors： Brookes SM,Cohen SB,Price EJ,Webb LM,Feldmann M,Maini RN,Venables PJ

    更新日期：1996-02-01 00:00:00

  • Impaired interleukin (IL)-4-associated generation of CCR4-expressing T cells in neonates with hereditary allergy risk.

    abstract：:Reduced microbial exposure in early life may contribute to the increase of atopic diseases in 'westernized' societies but the underlying mechanisms remain elusive. The objective of this study was to examine how exposure to bacterial lipopolysaccharide (LPS) during early antigen encounter might influence the maturation...

    journal_title：Clinical and experimental immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2249.2005.02706.x

    authors： Haddeland U,Sletten GB,Brandtzaeg P,Nakstad B

    更新日期：2005-02-01 00:00:00