Abstract：
:Purified proteins (Pa-1 and Pa-2) from pokeweed have been compared with commercial pokeweed mitogen (PWM-G) and other mitogens in their ability to stimulate human lymphocytes. With cultures of T and B cells separated from tonsil lymphocytes, thymidine uptake, blast transformation and immunoglobulin (Ig) synthesis have been measured. IgM and IgG was measured in supernates of stimulated cultures by radioimmunoassay. Pa-1, Pa-2 and PWM-G were found to be potent mitogens for unseparated tonsil lymphocytes or nylon column purified T cells. Pa-2 was found to be active at lower concentrations than Pa-1, and PWM-G was less potent than the purified mitogens. These three mitogens all stimulated unseparated lymphocytes to secrete large quantities of Ig (20-100 mug/ml) during 7 days in culture. With increasing amounts of mitogens severe decreases in immunoglobulin synthesis were observed at day 6 even with doses which were still optimal for stimulation of thymidine uptake at days 3 and 6. With purified B cells (less than 2% T cells) Pa-1 was the best mitogen for thymidine incorporation. However, the secretory response was very variable. In some experiments B cells did not secrete Ig in response to mitogens; in others Pa-1 was clearly more effective at stimulating secretion than Pa-2 or PWM-G and in some experiments B cells were stimulated by all three. In one experiment Pa-1 stimulated prolymphocytic leukaemia cells to blast transformation and the secretion of IgM. It is concluded that Pa-1, Pa-2 and PWM-G are much better activators of Ig synthesis in human cultures than either PHA or LPS and that Pa-1 is the most reliable B-cell stimulant of the three.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Janossy G,Gomez De La Concha E,Waxdal MJ,Platts-Mills Tsubject
Has Abstractpub_date
1976-10-01 00:00:00pages
108-17issue
1eissn
0009-9104issn
1365-2249journal_volume
26pub_type
杂志文章abstract：:Vaccination against meningococcal serogroup B is recommended for patients with a complement deficiency; however, although immunogenicity in this patient group has been shown, efficacy has not yet been established. In this study, we collected serum from children with a complement deficiency in the alternative pathway o...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13368
更新日期：2019-12-01 00:00:00
abstract：:Common variable immunodeficiency (CVID) is the name given to a clinically heterogeneous group of hypogammaglobulinaemic immunodeficiency states. Bronchiectasis is a feature of this disease and is believed to be the result of recurrent bacterial infection affecting the respiratory tract. Bronchiectasis is also a featur...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01995.x
更新日期：2002-12-01 00:00:00
abstract：:The value of the MLR in predicting graft outcome was evaluated in forty cadaveric grafts. The MLR was assessed by the one and two way stimulation index (SI), the relative response (RR), and the stabilized relative response (SRR). Graft function was assessed by function at 3 and 6 months after transplantation, and a re...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Growth of a methylcholanthrene-induced fibrosarcoma in BALB/c mice was accompanied by an increase in the activation state of tumour-associated macrophages (TAM), as measured by their FcIgG receptor expression, phagocytic index and beta-glucuronidase levels. All of these parameters were markedly higher in TAM than in p...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05816.x
更新日期：1991-11-01 00:00:00
abstract：:The role of T cells in the pathophysiology of chronic obstructive pulmonary disease (COPD) is not yet certain, although varying reports have shown increases in T helper 1 (Th1) and/or Th2 cytokines in peripheral blood and bronchoalveolar lavage (BAL). No studies have examined cytokine production by intraepithelial T c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2007.03451.x
更新日期：2007-10-01 00:00:00
abstract：:Specific assays for 5'-nucleotidase, adenosine diphosphatase (ADPase) and Mg2+-dependent adenosine triphosphatase (Mg2+-ATPase) have been optimized for human lymphocytes and their subcellular localizations determined by sucrose density gradient centrifugation. 5'-Nucleotidase was localized solely to the plasma membran...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-08-01 00:00:00
abstract：:A complete functional deficiency of C1q is described in a patient suffering from SLE. From reduced plasma C1 activity of the parents a hereditary trait was assumed. The defective C1q molecule was haemolytically inactive, did not bind to immune complexes, and was not recognized by the monocyte C1q receptor. C1 activity...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb03442.x
更新日期：1993-11-01 00:00:00
abstract：:This study was undertaken to determine if Brazilian Pemphigus foliaceus (BPF) autoantibodies will fix complement as do P. vulgaris (PV) autoantibodies. When sections of human skin were examined following indirect immunofluorescence (IF) staining, BPF autoantibodies reacted with the upper layers of epidermis, while PV ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
abstract：:In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril preparations, including three from patients with amyloidosis secondary to lepromatous leprosy and one from an unusual juvenile c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:Human immunodeficiency virus (HIV)-1 protease is a known target of CD8+ T cell responses, but it is the only HIV-1 protein in which no fully characterized HIV-1 protease CD4 epitopes have been identified to date. We investigated the recognition of HIV-1 protease by CD4+ T cells from 75 HIV-1-infected, protease inhibit...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04319.x
更新日期：2011-04-01 00:00:00
abstract：:One of the hallmarks of coeliac disease, both active and treated, is an increased number and proportion of gamma/delta intraepithelial T lymphocytes in the small intestinal mucosa, and an increased number of gamma/delta T cells in the small intestinal mucosa of coeliac disease patients has been associated with the inh...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1997.d01-887.x
更新日期：1997-01-01 00:00:00
abstract：:The synthesis of some class 1 acute-phase proteins (APP), including C-reactive protein (CRP) and serum amyloid A (SAA) protein is completely blocked by the IL-1 receptor antagonist (IL-1Ra), whereas the production of fibrinogen, a class 2 APP, is increased by IL-1Ra in hepatoma cells, but this has never been tested in...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb03669.x
更新日期：1995-05-01 00:00:00
abstract：:Activation of the interferon (IFN) system is an early defence mechanism against viral infections. The virus stimulates production of IFN by nucleated cells including the peripheral blood mononuclear cells (PBMC), and this IFN in turn activates several IFN-dependent immune mechanisms including the induction of an anti-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-05-01 00:00:00
abstract：:The c-Jun N-terminal kinase (JNK) participates in intracellular signalling cascades that mediate inflammatory responses. Therefore, the JNK signalling may be involved in gastric injury and inhibition of this pathway may form the basis of a new strategy for the treatment of gastric injury. The aim of this study was to ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02959.x
更新日期：2006-01-01 00:00:00
abstract：:Several parameters of lymphocyte interleukin 2 receptor (IL-2R) expression in response to tetanus toxoid (TT) were monitored using a receptor-specific monoclonal antibody and flow cytometry in patients with acquired immunodeficiency syndrome (AIDS, n = 6), AIDS-related complex (ARC, n = 8), or lymphadenopathy syndrome...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:Intraperitoneal infection of mice with mycobacteria induces the persistent mobilization of neutrophils to the infected peritoneal cavities. The recruitment of the neutrophils was mediated by the immune system since it was enhanced by immunization and reduced in T cell-deficient nude and SCID mice. Anti-mitotic treatme...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb06889.x
更新日期：1992-07-01 00:00:00
abstract：:The risk of premature death is high in haemodialysis (HD) patients. Antibodies against cardiolipin (anti-CL) are thrombogenic in diseases such as systemic lupus erythematosus (SLE). CL is easily oxidized (Ox) and plays a role in apoptosis. In this work we studied immunoglobulin (Ig)M anti-CL and anti-OxCL in HD-patien...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12181
更新日期：2013-12-01 00:00:00
abstract：:A new antimitochondrial antibody (AMA) against an outer membrane associated antigen on liver mitochondria was detected by ELISA in sera from patients with primary biliary cirrhosis (PBC). This antibody was named anti-M9. There is evidence that it is a partial organ-specific antibody as shown by absorption studies usin...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-10-01 00:00:00
abstract：:EAU is a model of ocular inflammatory disease. EAU resembles another T cell-mediated autoimmune disease--experimental allergic encephalomyelitis--since both have increased expression of MHC class II molecules in the target tissue, can be adoptively transferred by activated CD4+ T cells and are inhibited by cyclosporin...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.1992.tb06926.x
更新日期：1992-08-01 00:00:00
abstract：:Using a sensitive IL-1-dependent T cell clone we estimated interleukin 1 (IL-1)-like activity in a variety of synovial fluids (SF). Although we demonstrate that SF contains potent inhibitors of endogenous and exogenous IL-1 we were able to estimate that samples from rheumatoid arthritis, osteoarthritis and other arthr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:Parasite antigens released from Trypanosoma cruzi-infected cells were adsorbed to infected and uninfected mammalian cells thus rendering them susceptible to immune lysis by antibody and cell-mediated immunity directed against the parasite. BALB/c mice infected with T. cruzi for 15 days developed cytotoxic T lymphocyte...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:HAART may increase CD4+ T cell counts despite a persistently detectable HIV load. The impact of HAART on apoptosis, which may play a role in the disease process in HIV-infected patients, has not been extensively studied. We performed a study to compare the level of spontaneous T cell apoptosis and anti-retroviral trea...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.01076.x
更新日期：1999-12-01 00:00:00
abstract：:Experimental autoimmune hepatitis (EAH) is an animal model for autoimmune hepatitis. The disease is T cell-mediated and runs a subacute course, with maximal disease activity around week four after disease induction and a slow ensuing recovery. The aim of the present study was to investigate the immunoregulatory mechan...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb05995.x
更新日期：1993-10-01 00:00:00
abstract：:Members of the European Society for Immunodeficiencies (ESID) and other colleagues have updated the multi-stage expert-opinion-based diagnostic protocol for non-immunologists incorporating newly defined primary immunodeficiency diseases (PIDs). The protocol presented here aims to increase the awareness of PIDs among d...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2249.2011.04461.x
更新日期：2012-01-01 00:00:00
abstract：:Protein kinases mediate protein phosphorylation, which is a fundamental component of cell signalling, with crucial roles in most signal transduction cascades: from controlling cell growth and proliferation to the initiation and regulation of immunological responses. Aberrant kinase activity is implicated in an increas...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
doi：10.1111/cei.12248
更新日期：2014-04-01 00:00:00
abstract：:The functional integrity of the local immune system in vitamin A-deficient (A-) rats was investigated. Secretory IgA levels in the intestinal fluid of A- rats were significantly lower than in controls. This and the decrease in intensity of immunofluorescent staining for secretory component (SC) in the intestinal cells...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Recent evidence suggests that T cell apoptosis could be involved in the pathogenesis of HIV-1 infection. As the progression of HIV-2 associated disease appears to be slower than that of HIV-1, we investigated whether there were differences in the degree of T cell death and apoptosis in peripheral blood mononuclear cel...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1994.tb06123.x
更新日期：1994-11-01 00:00:00
abstract：:The recent development of salivary proteomics has led to the identification of potential biomarkers for diagnosing patients with primary Sjögren's syndrome (pSS). Here we sought to identify differentially produced salivary metabolites from pSS patients and healthy controls (HCs) that might be used to characterize this...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12683
更新日期：2015-11-01 00:00:00
abstract：:Expression of CD4 or CD8 on the cell surface is an important guide for discriminating the immunological functions of T cells. However, a minor T cell subset, which lacks both CD4 and CD8 molecules but bears the usual form of T cell receptor (TCR) alpha beta (CD4-CD8-TCR alpha beta+ T cells), has recently been found no...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05761.x
更新日期：1991-09-01 00:00:00
abstract：:A more complete understanding of immune-mediated damage to the coronary arteries in children with Kawasaki disease (KD) is required for improvements in patient treatment and outcomes. We recently reported the transcriptional profile of KD coronary arteritis, and in this study sought to determine protein expression of ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13010
更新日期：2017-11-01 00:00:00
