Abstract:
:Sites of implantation with compromised biology may be unable to achieve the required level of angiogenic and osteogenic regeneration. The specific function and contribution of different cell types to the formation of prevascularized, osteogenic networks in co-culture remains unclear. To determine how bone marrow-derived mesenchymal stromal cells (BMSCs) and endothelial cells (ECs) contribute to cellular proangiogenic differentiation, we analysed the differentiation of BMSCs and ECs in standardized monolayer, Transwell and co-cultures. BMSCs were derived from the iliac bone marrow of five patients, characterized and differentiated in standardized monolayers, permeable Transwells and co-cultures with human umbilical vein ECs (HUVECs). The expression levels of CD31, von Willebrand factor, osteonectin (ON) and Runx2 were assessed by quantitative reverse transcriptase polymerase chain reaction. The protein expression of alkaline phosphatase, ON and CD31 was demonstrated via histochemical and immunofluorescence analysis. The results showed that BMSCs and HUVECs were able to retain their lineage-specific osteogenic and angiogenic differentiation in direct and indirect co-cultures. In addition, BMSCs demonstrated a supportive expression of angiogenic function in co-culture, while HUVEC was able to improve the expression of osteogenic marker molecules in BMSCs.
journal_name
Int J Oral Scijournal_title
International journal of oral scienceauthors
Böhrnsen F,Schliephake Hdoi
10.1038/ijos.2016.39subject
Has Abstractpub_date
2016-12-16 00:00:00pages
223-230issue
4eissn
1674-2818issn
2049-3169pii
ijos201639journal_volume
8pub_type
杂志文章abstract::Dentin matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes trapped within mineralized dentin matrix, which have the ability to hydrolyze the organic matrix of demineralized dentin. After bonding with resins to dentin there are usually some exposed collagen fibrils at the bottom of the hyb...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS.09044
更新日期:2009-12-01 00:00:00
abstract:AIM:The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. METHODOLOGY:Ten subjects (6 women, 4 men; mean age 25.4 years) participated in the study. The mandibular movement, sagittal condylar inclination angle, and transversal condylar inclination angle of each subj...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS10071
更新日期:2010-12-01 00:00:00
abstract::Halitosis, bad breath or oral malodour are all synonyms for the same pathology. Halitosis has a large social and economic impact. For the majority of patients suffering from bad breath, it causes embarrassment and affects their social communication and life. Moreover,halitosis can be indicative of underlying diseases....
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/ijos.2012.39
更新日期:2012-06-01 00:00:00
abstract::Bone remodelling keeps going through the lifespan of human by bone formation and bone resorption. In the craniofacial region, mandibles act as the main force for biting and chewing, and also become susceptible to a common bone-loss disease, namely, apical periodontitis, once infected dental pulp is not treated timely,...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-019-0058-x
更新日期:2019-08-26 00:00:00
abstract::By removing a part of the structure, the tooth preparation provides restorative space, bonding surface, and finish line for various restorations on abutment. Preparation technique plays critical role in achieving the optimal result of tooth preparation. With successful application of microscope in endodontics for >30 ...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-019-0057-y
更新日期:2019-10-02 00:00:00
abstract::Optical spectroscopy devices are being developed and tested for the screening and diagnosis of oral precancer and cancer lesions. This study reports a device that uses white light for detection of suspicious lesions and green-amber light at 545 nm that detect tissue vascularity on patients with several suspicious oral...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.39
更新日期:2014-09-01 00:00:00
abstract::Although a few studies have shown that vascularity is increased from normal mucosa to dysplasia to carcinoma suggesting that disease progression in the oral mucosa is accompanied by angiogenesis. The role in lymph node metastasis in oral squamous cell carcinoma (OSCC) is equivocal. Role of angiogenesis in OSCC develop...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS11077
更新日期:2011-10-01 00:00:00
abstract::Drug-induced gingival overgrowth (DIGO) is recognized as a side effect of nifedipine (NIF); however, the underlying molecular mechanisms remain unknown. In this study, we found that overexpressed miR-4651 inhibits cell proliferation and induces G0/G1-phase arrest in gingival mesenchymal stem cells (GMSCs) with or with...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-020-0076-8
更新日期:2020-03-31 00:00:00
abstract::Secondary caries due to biofilm acids is a primary cause of dental composite restoration failure. To date, there have been no reports of dental composites that can repel protein adsorption and inhibit bacteria attachment. The objectives of this study were to develop a protein-repellent dental composite by incorporatin...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.77
更新日期:2015-06-26 00:00:00
abstract::Osteoporosis is a serious public bone metabolic disease. However, the mechanisms underlying bone loss combined with ageing, which is known as senile osteoporosis, remains unknown. Here we show the detailed phenotype of this disease caused by SIRT6 knock out (KO) in mice. To the best of our knowledge, this is the first...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2015.57
更新日期:2016-06-30 00:00:00
abstract::Biofilms at the tooth-restoration bonded interface can produce acids and cause recurrent caries. Recurrent caries is a primary reason for restoration failures. The objectives of this study were to synthesize a novel bioactive dental bonding agent containing dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacrylo...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-018-0019-9
更新日期:2018-06-21 00:00:00
abstract::Retention wires permanently bonded to the anterior teeth are used after orthodontic treatment to prevent the teeth from relapsing to pre-treatment positions. A disadvantage of bonded retainers is biofilm accumulation on the wires, which produces a higher incidence of gingival recession, increased pocket depth and blee...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.69
更新日期:2015-03-23 00:00:00
abstract::To evaluate the feasibility and accuracy of a three-dimensional augmented reality system incorporating integral videography for imaging oral and maxillofacial regions, based on preoperative computed tomography data. Three-dimensional surface models of the jawbones, based on the computed tomography data, were used to c...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.26
更新日期:2013-06-01 00:00:00
abstract::The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO2 nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-deri...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-020-00103-3
更新日期:2020-12-30 00:00:00
abstract::In this study, we evaluate the influence of post surface pre-treatments on the bond strength of four different cements to glass fiber posts. Eighty extracted human maxillary central incisors and canines were endodontically treated and standardized post spaces were prepared. Four post pre-treatments were tested: (i) no...
journal_title:International journal of oral science
pub_type: 杂志文章,随机对照试验
doi:10.1038/ijos.2013.83
更新日期:2014-03-01 00:00:00
abstract:AIM:To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. METHODOLOGY:Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growt...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/ijos.08025
更新日期:2009-06-01 00:00:00
abstract::This study aimed to compare epithelial cells derived from human embryonic stem cells (hESCs) to human ameloblast-lineage cells (ALCs), as a way to determine their potential use as a cell source for ameloblast regeneration. Induced by various concentrations of bone morphogenetic protein 4 (BMP4), retinoic acid (RA) and...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.14
更新日期:2013-03-01 00:00:00
abstract::When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dediff...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS11044
更新日期:2011-07-01 00:00:00
abstract::Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulatory morphogens that contro...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.41
更新日期:2013-06-01 00:00:00
abstract::All-trans retinoic acid (ATRA) inhibits matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fibroblasts, skin fibroblasts, bronchoalveolar lavage cells and cancer cells, but activates MMP-9 in neuroblast and leukemia cells. Very little is known regarding whether ATRA can activate or inhibit MMPs in human dental pul...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.63
更新日期:2014-09-01 00:00:00
abstract::The aim of the present real time in vivo micro-computed tomography (µCT) and histologic experiment was to assess the efficacy of guided bone regeneration (GBR) around standardized calvarial critical size defects (CSD) using bone marrow-derived mesenchymal stem cells (BMSCs), and collagen membrane (CM) with and without...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2015.34
更新日期:2016-03-30 00:00:00
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journal_title:International journal of oral science
pub_type: 杂志文章
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更新日期:2015-06-26 00:00:00
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journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS.09032
更新日期:2009-09-01 00:00:00
abstract::The aim of the study was a determination of the levels of nitric oxide (NO) and its biological markers such as malonyldialdehyde (MDA) and nitrotyrosine in the serum of patients with squamous cell carcinoma (SCC) of the oral cavity and identification of the relationships between NO and those markers. These studies wer...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.59
更新日期:2013-09-01 00:00:00
abstract::Bacterial biofilms can be viewed as a specific type of persistent bacterial infection. After initial invasion, microbes can attach to living and non-living surfaces, such as prosthetics and indwelling medical devices, and form a biofilm composed of extracellular polysaccharides, proteins, and other components. In host...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS11022
更新日期:2011-04-01 00:00:00
abstract::The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to ma...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2012.22
更新日期:2012-06-01 00:00:00
abstract::Tooth decay is prevalent, and secondary caries causes restoration failures, both of which are related to demineralization. There is an urgent need to develop new therapeutic materials with remineralization functions. This article represents the first review on the cutting edge research of poly(amido amine) (PAMAM) in ...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/s41368-019-0048-z
更新日期:2019-05-09 00:00:00
abstract::The human microbiome functions as an intricate and coordinated microbial network, residing throughout the mucosal surfaces of the skin, oral cavity, gastrointestinal tract, respiratory tract, and reproductive system. The oral microbiome encompasses a highly diverse microbiota, consisting of over 700 microorganisms, in...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/s41368-020-0082-x
更新日期:2020-04-30 00:00:00
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journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-020-0079-5
更新日期:2020-05-09 00:00:00
abstract::Along with the development of new materials, advanced medical imaging and surgical techniques, osseointegrated dental implants are considered a successful and constantly evolving treatment modality for the replacement of missing teeth in patients with complete or partial edentulism. The importance of restoring the per...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/ijos.2017.1
更新日期:2017-03-01 00:00:00