DAPT enhances the apoptosis of human tongue carcinoma cells.

Abstract:

AIM:To investigate the effect of DAPT (gamma-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. METHODOLOGY:Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence (IF) were employed to determine the intracellular expression levels. RESULTS:DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis. The mRNA levels of Hairy/Enhancer of Split-1 (Hes-1), a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. CONCLUSION:DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3.

journal_name

Int J Oral Sci

authors

Grottkau BE,Chen XR,Friedrich CC,Yang XM,Jing W,Wu Y,Cai XX,Liu YR,Huang YD,Lin YF

doi

10.4248/ijos.08025

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

81-9

issue

2

eissn

1674-2818

issn

2049-3169

journal_volume

1

pub_type

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