Abstract:
:When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dedifferentiated into lipid-free fibroblast-like cells, named dedifferentiated fat (DFAT) cells. DFAT cells re-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells are introduced and the current profiles of their cellular nature are summarized. Under proper induction culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenic and myogenic potentials. In angiogenic conditions, DFAT cells could exhibit perivascular characteristics and elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine.
journal_name
Int J Oral Scijournal_title
International journal of oral scienceauthors
Shen JF,Sugawara A,Yamashita J,Ogura H,Sato Sdoi
10.4248/IJOS11044subject
Has Abstractpub_date
2011-07-01 00:00:00pages
117-24issue
3eissn
1674-2818issn
2049-3169journal_volume
3pub_type
杂志文章,评审abstract::After teeth are replanted, there are two possible healing responses: periodontal ligament healing or ankylosis with subsequent replacement resorption. The purpose of this study was to compare the fatigue resistance of vertically fractured teeth after bonding the fragments under conditions simulating both healing modes...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.51
更新日期:2015-06-26 00:00:00
abstract::Bone remodelling keeps going through the lifespan of human by bone formation and bone resorption. In the craniofacial region, mandibles act as the main force for biting and chewing, and also become susceptible to a common bone-loss disease, namely, apical periodontitis, once infected dental pulp is not treated timely,...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-019-0058-x
更新日期:2019-08-26 00:00:00
abstract::For a long time, oral disease is one of the major problems of the public health for its high prevalence and incidence throughout the world, which is especially true for low-income populations. Since China's economic reform in 1978, great changes have taken place in China. These changes have significant impact on and h...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS11006
更新日期:2011-01-01 00:00:00
abstract::Dentin matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes trapped within mineralized dentin matrix, which have the ability to hydrolyze the organic matrix of demineralized dentin. After bonding with resins to dentin there are usually some exposed collagen fibrils at the bottom of the hyb...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS.09044
更新日期:2009-12-01 00:00:00
abstract::Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects and digestion. With the rapid advancement in salivaomics, saliva is well recognized as a pool of biological markers. Saliva, as a non-invasive and safe source, could be a substitute fo...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/ijos.2016.38
更新日期:2016-09-29 00:00:00
abstract::A previous study demonstrated that alexidine has greater affinity for the major virulence factors of bacteria than chlorhexidine. The aim of this study was to compare the antimicrobial activity of 1% alexidine with that of 2% chlorhexidine using Enterococcus faecalis-infected dentin blocks. Sixty bovine dentin blocks ...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.11
更新日期:2013-03-01 00:00:00
abstract::Although a few studies have shown that vascularity is increased from normal mucosa to dysplasia to carcinoma suggesting that disease progression in the oral mucosa is accompanied by angiogenesis. The role in lymph node metastasis in oral squamous cell carcinoma (OSCC) is equivocal. Role of angiogenesis in OSCC develop...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS11077
更新日期:2011-10-01 00:00:00
abstract::In this study, we evaluate the influence of post surface pre-treatments on the bond strength of four different cements to glass fiber posts. Eighty extracted human maxillary central incisors and canines were endodontically treated and standardized post spaces were prepared. Four post pre-treatments were tested: (i) no...
journal_title:International journal of oral science
pub_type: 杂志文章,随机对照试验
doi:10.1038/ijos.2013.83
更新日期:2014-03-01 00:00:00
abstract::To modify two-step experimental etch-and-rinse dentin adhesive with different concentrations of riboflavin and to study its effect on the bond strength, degree of conversion, along with resin infiltration within the demineralized dentin substrate, an experimental adhesive-system was modified with different concentrati...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.49
更新日期:2015-06-26 00:00:00
abstract::Along with the development of new materials, advanced medical imaging and surgical techniques, osseointegrated dental implants are considered a successful and constantly evolving treatment modality for the replacement of missing teeth in patients with complete or partial edentulism. The importance of restoring the per...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/ijos.2017.1
更新日期:2017-03-01 00:00:00
abstract::Tooth development is a complex process that involves precise and time-dependent orchestration of multiple genetic, molecular, and cellular interactions. Ameloblastin (AMBN, also named "amelin" or "sheathlin") is the second most abundant enamel matrix protein known to have a key role in amelogenesis. Amelogenesis imper...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-018-0027-9
更新日期:2018-09-03 00:00:00
abstract::Bacterial biofilms can be viewed as a specific type of persistent bacterial infection. After initial invasion, microbes can attach to living and non-living surfaces, such as prosthetics and indwelling medical devices, and form a biofilm composed of extracellular polysaccharides, proteins, and other components. In host...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS11022
更新日期:2011-04-01 00:00:00
abstract::The aim of the study was a determination of the levels of nitric oxide (NO) and its biological markers such as malonyldialdehyde (MDA) and nitrotyrosine in the serum of patients with squamous cell carcinoma (SCC) of the oral cavity and identification of the relationships between NO and those markers. These studies wer...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.59
更新日期:2013-09-01 00:00:00
abstract::Osteoporosis is a serious public bone metabolic disease. However, the mechanisms underlying bone loss combined with ageing, which is known as senile osteoporosis, remains unknown. Here we show the detailed phenotype of this disease caused by SIRT6 knock out (KO) in mice. To the best of our knowledge, this is the first...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2015.57
更新日期:2016-06-30 00:00:00
abstract::Sites of implantation with compromised biology may be unable to achieve the required level of angiogenic and osteogenic regeneration. The specific function and contribution of different cell types to the formation of prevascularized, osteogenic networks in co-culture remains unclear. To determine how bone marrow-deriv...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2016.39
更新日期:2016-12-16 00:00:00
abstract::This in vitro study aims to evaluate the crystal and surface microstructure of dental enamel after cold-light bleaching treatment. Twelve sound human premolars were cross-split into four specimens, namely, mesio-buccal (Group LP), disto-buccal (Group P), mesio-lingual (Group NP) and disto-lingual (Group L) specimens. ...
journal_title:International journal of oral science
pub_type: 杂志文章,随机对照试验
doi:10.1038/ijos.2012.70
更新日期:2012-12-01 00:00:00
abstract::The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO2 nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-deri...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-020-00103-3
更新日期:2020-12-30 00:00:00
abstract::Both bone morphogenetic protein 2 (BMP2) and the wingless-type MMTV integration site (WNT)/β-catenin signalling pathway play important roles in odontoblast differentiation and dentinogenesis. Cross-talk between BMP2 and WNT/β-catenin in osteoblast differentiation and bone formation has been identified. However, the ro...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2015.7
更新日期:2015-06-26 00:00:00
abstract:AIM:To examine what impact the loss of funding had on the utilization of the oral pathology service. METHODOLOGY:Biopsy records were retrieved and examined in the two year period before and after the elimination of the subsidies in 2003. RESULTS:After the loss of funding, there was a 31% decrease in the number of spe...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS.09032
更新日期:2009-09-01 00:00:00
abstract::By removing a part of the structure, the tooth preparation provides restorative space, bonding surface, and finish line for various restorations on abutment. Preparation technique plays critical role in achieving the optimal result of tooth preparation. With successful application of microscope in endodontics for >30 ...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-019-0057-y
更新日期:2019-10-02 00:00:00
abstract::The aim of the present real time in vivo micro-computed tomography (µCT) and histologic experiment was to assess the efficacy of guided bone regeneration (GBR) around standardized calvarial critical size defects (CSD) using bone marrow-derived mesenchymal stem cells (BMSCs), and collagen membrane (CM) with and without...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2015.34
更新日期:2016-03-30 00:00:00
abstract:AIM:To evaluate the effect of single or dual field irradiation (IR) with the same dose on damage to miniature pig parotid glands. METHODOLOGY:Sixteen miniature pigs were divided into two IR groups (n=6) and a control group (n=4). The irradiation groups were subjected to 20 Gy X-radiation to one parotid gland using sin...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/ijos.08001
更新日期:2009-03-01 00:00:00
abstract::Bone defects caused by trauma, tumour resection, infection and congenital deformities, together with articular cartilage defects and cartilage-subchondral bone complex defects caused by trauma and degenerative diseases, remain great challenges for clinicians. Novel strategies utilising cell sheet technology to enhance...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/s41368-019-0050-5
更新日期:2019-05-21 00:00:00
abstract::All-trans retinoic acid (ATRA) inhibits matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fibroblasts, skin fibroblasts, bronchoalveolar lavage cells and cancer cells, but activates MMP-9 in neuroblast and leukemia cells. Very little is known regarding whether ATRA can activate or inhibit MMPs in human dental pul...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2013.63
更新日期:2014-09-01 00:00:00
abstract::Optical spectroscopy devices are being developed and tested for the screening and diagnosis of oral precancer and cancer lesions. This study reports a device that uses white light for detection of suspicious lesions and green-amber light at 545 nm that detect tissue vascularity on patients with several suspicious oral...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/ijos.2014.39
更新日期:2014-09-01 00:00:00
abstract::The oral microbial community is widely regarded as a latent reservoir of antibiotic resistance genes. This study assessed the molecular epidemiology, susceptibility profile, and resistance mechanisms of 35 methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from the dental plaque of a healthy huma...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-020-0079-5
更新日期:2020-05-09 00:00:00
abstract:AIM:The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. METHODOLOGY:Ten subjects (6 women, 4 men; mean age 25.4 years) participated in the study. The mandibular movement, sagittal condylar inclination angle, and transversal condylar inclination angle of each subj...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.4248/IJOS10071
更新日期:2010-12-01 00:00:00
abstract::Tooth decay is prevalent, and secondary caries causes restoration failures, both of which are related to demineralization. There is an urgent need to develop new therapeutic materials with remineralization functions. This article represents the first review on the cutting edge research of poly(amido amine) (PAMAM) in ...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.1038/s41368-019-0048-z
更新日期:2019-05-09 00:00:00
abstract::Biofilms at the tooth-restoration bonded interface can produce acids and cause recurrent caries. Recurrent caries is a primary reason for restoration failures. The objectives of this study were to synthesize a novel bioactive dental bonding agent containing dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacrylo...
journal_title:International journal of oral science
pub_type: 杂志文章
doi:10.1038/s41368-018-0019-9
更新日期:2018-06-21 00:00:00
abstract::Tissue engineering allows the design of functionally active cells within supportive bio-scaffolds to promote the development of new tissues such as cartilage and bone for the restoration of pathologically altered tissues. However, all bone tissue engineering applications are limited by a shortage of stem cells. The ad...
journal_title:International journal of oral science
pub_type: 杂志文章,评审
doi:10.4248/IJOS10045
更新日期:2010-09-01 00:00:00