Abstract：
:The aim of this study was to evaluate the biological efficacy of a unique perpendicular protrusion of type-I collagen (Col-I) from TiO2 nanotubes (NT-EPF surface). We hypothesized that the NT-EPF surface would play bifunctional roles in stimulating platelet-mediated fibroblast recruitment and anchoring fibroblast-derived Col-I to form a perpendicular collagen assembly, mimicking the connective tissue attachment around natural teeth for the long-term maintenance of dental implants. Ti surface modification was accomplished in two steps. First, TiO2 nanotubes (NT) array was fabricated via anodization. Diameters and depths of NTs were controlled by applied voltage and duration. Subsequently, an electrophoretic fusion (EPF) method was applied to fuse Col-I into nanotube arrays in a perpendicular fashion. Surface wettability was assessed by contact angle measurement. The bioactivity of modified TiO2 surfaces was evaluated in terms of NIH3T3 fibroblast attachment, platelet activation, and collagen extension. Early attachment, aggregation, and activation of platelets as well as release of platelet-related growth factors were demonstrated on NT-EPF surfaces. Platelet-mediated NIH3T3 cells migration toward NT-EPF was significantly increased and the attached cells showed a typical fibrous morphology with elongated spindle shape. A direct linkage between pseudopod-like processes of fibroblasts to NT-EPF surfaces was observed. Furthermore, the engineered EPF collagen protrusion linked with cell-derived collagen in a perpendicular fashion. Within the limitation of this in vitro study, the TiO2 nanotube with perpendicular Col-I surface (NT-EPF) promoted better cell attachment, induced a strong platelet activation which suggested the ability to create a more robust soft tissue seal.
journal_name
Int J Oral Scijournal_title
International journal of oral scienceauthors
Chen CY,Kim DM,Lee C,Da Silva J,Nagai S,Nojiri T,Nagai Mdoi
10.1038/s41368-020-00103-3subject
Has Abstractpub_date
2020-12-30 00:00:00pages
36issue
1eissn
1674-2818issn
2049-3169pii
10.1038/s41368-020-00103-3journal_volume
12pub_type
杂志文章abstract：:The aim of the present real time in vivo micro-computed tomography (µCT) and histologic experiment was to assess the efficacy of guided bone regeneration (GBR) around standardized calvarial critical size defects (CSD) using bone marrow-derived mesenchymal stem cells (BMSCs), and collagen membrane (CM) with and without...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2015.34
更新日期：2016-03-30 00:00:00
abstract：:Formation of the periodontium begins following onset of tooth-root formation in a coordinated manner after birth. Dental follicle progenitor cells are thought to form the cementum, alveolar bone and Sharpey's fibers of the periodontal ligament (PDL). However, little is known about the regulatory morphogens that contro...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2013.41
更新日期：2013-06-01 00:00:00
abstract：:This in vitro study aims to evaluate the crystal and surface microstructure of dental enamel after cold-light bleaching treatment. Twelve sound human premolars were cross-split into four specimens, namely, mesio-buccal (Group LP), disto-buccal (Group P), mesio-lingual (Group NP) and disto-lingual (Group L) specimens. ...
journal_title：International journal of oral science
pub_type： 杂志文章,随机对照试验
doi：10.1038/ijos.2012.70
更新日期：2012-12-01 00:00:00
abstract：:All-trans retinoic acid (ATRA) inhibits matrix metalloproteinase (MMP)-2 and MMP-9 in synovial fibroblasts, skin fibroblasts, bronchoalveolar lavage cells and cancer cells, but activates MMP-9 in neuroblast and leukemia cells. Very little is known regarding whether ATRA can activate or inhibit MMPs in human dental pul...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2013.63
更新日期：2014-09-01 00:00:00
abstract：:Drug-induced gingival overgrowth (DIGO) is recognized as a side effect of nifedipine (NIF); however, the underlying molecular mechanisms remain unknown. In this study, we found that overexpressed miR-4651 inhibits cell proliferation and induces G0/G1-phase arrest in gingival mesenchymal stem cells (GMSCs) with or with...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/s41368-020-0076-8
更新日期：2020-03-31 00:00:00
abstract：:It is known that human papillomavirus (HPV) infection can cause squamous cell neoplasms at several sites, such as cervix uteri carcinoma and oral squamous carcinoma. There is little information on the expression of HPV and its predictive markers in tumours of the major and minor salivary glands of the head and neck. W...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2014.28
更新日期：2015-03-23 00:00:00
abstract：AIM:The purpose of this study is to compare the effects of the two clutches on recording the condylar movement. METHODOLOGY:Ten subjects (6 women, 4 men; mean age 25.4 years) participated in the study. The mandibular movement, sagittal condylar inclination angle, and transversal condylar inclination angle of each subj...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.4248/IJOS10071
更新日期：2010-12-01 00:00:00
abstract：:After teeth are replanted, there are two possible healing responses: periodontal ligament healing or ankylosis with subsequent replacement resorption. The purpose of this study was to compare the fatigue resistance of vertically fractured teeth after bonding the fragments under conditions simulating both healing modes...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2014.51
更新日期：2015-06-26 00:00:00
abstract：:When adipose-derived stem cells (ASCs) are retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dediff...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.4248/IJOS11044
更新日期：2011-07-01 00:00:00
abstract：:For a long time, oral disease is one of the major problems of the public health for its high prevalence and incidence throughout the world, which is especially true for low-income populations. Since China's economic reform in 1978, great changes have taken place in China. These changes have significant impact on and h...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.4248/IJOS11006
更新日期：2011-01-01 00:00:00
abstract：:The human microbiome functions as an intricate and coordinated microbial network, residing throughout the mucosal surfaces of the skin, oral cavity, gastrointestinal tract, respiratory tract, and reproductive system. The oral microbiome encompasses a highly diverse microbiota, consisting of over 700 microorganisms, in...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/s41368-020-0082-x
更新日期：2020-04-30 00:00:00
abstract：:The root is crucial for the physiological function of the tooth, and a healthy root allows an artificial crown to function as required clinically. Tooth crown development has been studied intensively during the last few decades, but root development remains not well understood. Here we review the root development proc...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/ijos.2012.61
更新日期：2012-12-01 00:00:00
abstract：:Bone defects caused by trauma, tumour resection, infection and congenital deformities, together with articular cartilage defects and cartilage-subchondral bone complex defects caused by trauma and degenerative diseases, remain great challenges for clinicians. Novel strategies utilising cell sheet technology to enhance...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/s41368-019-0050-5
更新日期：2019-05-21 00:00:00
abstract：:Halitosis, bad breath or oral malodour are all synonyms for the same pathology. Halitosis has a large social and economic impact. For the majority of patients suffering from bad breath, it causes embarrassment and affects their social communication and life. Moreover,halitosis can be indicative of underlying diseases....
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/ijos.2012.39
更新日期：2012-06-01 00:00:00
abstract：:Saliva is secreted from the salivary glands and has multiple functions, including mouth cleaning and protection, antibacterial effects and digestion. With the rapid advancement in salivaomics, saliva is well recognized as a pool of biological markers. Saliva, as a non-invasive and safe source, could be a substitute fo...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/ijos.2016.38
更新日期：2016-09-29 00:00:00
abstract：:Tooth development is a complex process that involves precise and time-dependent orchestration of multiple genetic, molecular, and cellular interactions. Ameloblastin (AMBN, also named "amelin" or "sheathlin") is the second most abundant enamel matrix protein known to have a key role in amelogenesis. Amelogenesis imper...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/s41368-018-0027-9
更新日期：2018-09-03 00:00:00
abstract：:Bacterial biofilms can be viewed as a specific type of persistent bacterial infection. After initial invasion, microbes can attach to living and non-living surfaces, such as prosthetics and indwelling medical devices, and form a biofilm composed of extracellular polysaccharides, proteins, and other components. In host...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.4248/IJOS11022
更新日期：2011-04-01 00:00:00
abstract：:Apoptosis or programmed cell death plays an essential role in chemotherapy-induced tumor cell killing, and inducers of apoptosis are commonly used in cancer therapy. Treatment with Zelkova serrata extracts was performed in human gingival fibroblast (HGF), mouth epidermoid carcinoma cell (KB), lower gingival squamous c...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2012.14
更新日期：2012-06-01 00:00:00
abstract：:Biofilms at the tooth-restoration bonded interface can produce acids and cause recurrent caries. Recurrent caries is a primary reason for restoration failures. The objectives of this study were to synthesize a novel bioactive dental bonding agent containing dimethylaminohexadecyl methacrylate (DMAHDM) and 2-methacrylo...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/s41368-018-0019-9
更新日期：2018-06-21 00:00:00
abstract：:Effective control of oral biofilm infectious diseases represents a major global challenge. Microorganisms in biofilms exhibit increased drug tolerance compared with planktonic cells. The present review covers innovative antimicrobial strategies for controlling oral biofilm-related infections published predominantly ov...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/s41368-019-0062-1
更新日期：2019-10-01 00:00:00
abstract：:Secondary caries due to biofilm acids is a primary cause of dental composite restoration failure. To date, there have been no reports of dental composites that can repel protein adsorption and inhibit bacteria attachment. The objectives of this study were to develop a protein-repellent dental composite by incorporatin...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2014.77
更新日期：2015-06-26 00:00:00
abstract：:This study aimed to compare epithelial cells derived from human embryonic stem cells (hESCs) to human ameloblast-lineage cells (ALCs), as a way to determine their potential use as a cell source for ameloblast regeneration. Induced by various concentrations of bone morphogenetic protein 4 (BMP4), retinoic acid (RA) and...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2013.14
更新日期：2013-03-01 00:00:00
abstract：:Tooth decay is prevalent, and secondary caries causes restoration failures, both of which are related to demineralization. There is an urgent need to develop new therapeutic materials with remineralization functions. This article represents the first review on the cutting edge research of poly(amido amine) (PAMAM) in ...
journal_title：International journal of oral science
pub_type： 杂志文章,评审
doi：10.1038/s41368-019-0048-z
更新日期：2019-05-09 00:00:00
abstract：:To evaluate the feasibility and accuracy of a three-dimensional augmented reality system incorporating integral videography for imaging oral and maxillofacial regions, based on preoperative computed tomography data. Three-dimensional surface models of the jawbones, based on the computed tomography data, were used to c...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2013.26
更新日期：2013-06-01 00:00:00
abstract：AIM:To examine what impact the loss of funding had on the utilization of the oral pathology service. METHODOLOGY:Biopsy records were retrieved and examined in the two year period before and after the elimination of the subsidies in 2003. RESULTS:After the loss of funding, there was a 31% decrease in the number of spe...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.4248/IJOS.09032
更新日期：2009-09-01 00:00:00
abstract：:To modify two-step experimental etch-and-rinse dentin adhesive with different concentrations of riboflavin and to study its effect on the bond strength, degree of conversion, along with resin infiltration within the demineralized dentin substrate, an experimental adhesive-system was modified with different concentrati...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2014.49
更新日期：2015-06-26 00:00:00
abstract：:The presence of a large palatal or maxillary defect after partial or total maxillectomy for tumor, trauma or congenital deformation poses a challenge to prosthodontists, particularly when the use of an implant cannot be considered. This case report described the use of an air valve in a hollow silicone obturator to ma...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2012.22
更新日期：2012-06-01 00:00:00
abstract：:A previous study demonstrated that alexidine has greater affinity for the major virulence factors of bacteria than chlorhexidine. The aim of this study was to compare the antimicrobial activity of 1% alexidine with that of 2% chlorhexidine using Enterococcus faecalis-infected dentin blocks. Sixty bovine dentin blocks ...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2013.11
更新日期：2013-03-01 00:00:00
abstract：AIM:To evaluate the effect of single or dual field irradiation (IR) with the same dose on damage to miniature pig parotid glands. METHODOLOGY:Sixteen miniature pigs were divided into two IR groups (n=6) and a control group (n=4). The irradiation groups were subjected to 20 Gy X-radiation to one parotid gland using sin...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.4248/ijos.08001
更新日期：2009-03-01 00:00:00
abstract：:Both bone morphogenetic protein 2 (BMP2) and the wingless-type MMTV integration site (WNT)/β-catenin signalling pathway play important roles in odontoblast differentiation and dentinogenesis. Cross-talk between BMP2 and WNT/β-catenin in osteoblast differentiation and bone formation has been identified. However, the ro...
journal_title：International journal of oral science
pub_type： 杂志文章
doi：10.1038/ijos.2015.7
更新日期：2015-06-26 00:00:00
