Abstract：
:Treatment of tendon-bone junction injuries is a challenge because tendon-bone interface often heals poorly and the fibrocartilage zone, which reduces stress concentration, at the interface is not formed. In this study, we used a compound called kartogenin (KGN) with platelet-rich plasma (PRP) to induce the formation of fibrocartilage zone in a rat tendon graft-bone tunnel model. The experimental rats received KGN-PRP or PRP injections in the tendon graft-bone tunnel interface. The control group received saline. After 4, 8 and 12 weeks, Safranin O staining of the tendon graft-bone tunnels revealed abundant proteoglycans in the KGN-PRP group indicating the formation of cartilage-like transition zone. Immunohistochemical and immuno-fluorescence staining revealed collagen types I (Col-I) and II (Col-II) in the newly formed fibrocartilage zone. Both fibrocartilage zone formation and maturation were healing time dependent. In contrast, the PRP and saline control groups had no cartilage-like tissues and minimal Col-I and Col-II staining. Some gaps were also present in the saline control group. Finally, pull-out strength in the KGN-PRP-treated group at 8 weeks was 1.4-fold higher than the PRP-treated group and 1.6-fold higher than the saline control group. These findings indicate that KGN, with PRP as a carrier, promotes the formation of fibrocartilage zone between the tendon graft and bone interface. Thus, KGN-PRP may be used as a convenient cell-free therapy in clinics to promote fibrocartilage zone formation in rotator calf repair and anterior cruciate ligament reconstruction, thereby enhancing the mechanical strength of the tendon-bone interface and hence the clinical outcome of these procedures. Copyright © 2017 John Wiley & Sons, Ltd.
journal_name
J Tissue Eng Regen Medjournal_title
Journal of tissue engineering and regenerative medicineauthors
Zhou Y,Zhang J,Yang J,Narava M,Zhao G,Yuan T,Wu H,Zheng N,Hogan MV,Wang JHdoi
10.1002/term.2258subject
Has Abstractpub_date
2017-12-01 00:00:00pages
3445-3456issue
12eissn
1932-6254issn
1932-7005journal_volume
11pub_type
杂志文章abstract：:In order to examine the differentiation potential of the tenocytes expanded in our defined culture medium (reported previously) and the effect of sequential combination of the two culture conditions on human tenocytes, a two-dimensional and three-dimensional experimental approach was used. Human tenocytes were sequent...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1791
更新日期：2016-03-01 00:00:00
abstract：:Biodegradable porous scaffolds have been investigated as an alternative approach to current metal, ceramic, and polymer bone graft substitutes for lost or damaged bone tissues. Although there have been many studies investigating the effects of scaffold architecture on bone formation, many of these scaffolds were fabri...
journal_title：Journal of tissue engineering and regenerative medicine
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doi：10.1002/term.497
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.2326
更新日期：2018-01-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.110
更新日期：2008-10-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
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doi：10.1002/term.2595
更新日期：2018-03-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1653
更新日期：2015-09-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2936
更新日期：2019-10-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
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更新日期：2016-02-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
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更新日期：2016-09-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1837
更新日期：2016-10-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2512
更新日期：2018-03-01 00:00:00
abstract：:Dermal papilla cells (DPCs) interact with epithelial stem cells and induce hair folliculogenesis. Cell-based therapies using expanded DPCs for hair regeneration have been unsuccessful in humans. Two major challenges remain: first, expanded DPCs obtained from adult hair follicles have functional limitations; second, a ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.400
更新日期：2012-02-01 00:00:00
abstract：:Elastin, a major component of vascular matrices, critically determines vascular mechanics and maintains the quiescence of smooth muscle cells (SMCs). Attempts to regenerate elastin in elastin-compromised blood vessels using tissue-engineering approaches is limited by the unavailability of elastogenic cues to upregulat...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.70
更新日期：2008-03-01 00:00:00
abstract：:Cartilage tissue engineering is making progress, but the competing available strategies still leave room for improvement and consensual overviews regarding the best combinations of scaffolds and cell sources are limited by the capacity to compare them directly. In addition, because most strategies involve autologous c...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2454
更新日期：2018-03-01 00:00:00
abstract：:Enhancement of osteogenic capacity was achieved in a mineralized collagen composite, nano-hydroxyapatite/collagen (nHAC), by loading with synthetic peptides derived from BMP-2 residues 32-48 (P17-BMP-2). Rabbit marrow stromal cells (MSCs) were used in vitro to study cell biocompatibility, attachment and differentiatio...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1705
更新日期：2016-02-01 00:00:00
abstract：:Macroporous calcium phosphate cement (CPC) with stem cell seeding is promising for bone regeneration. The objective of this study was to investigate the effects of co-delivering autologous bone marrow mesenchymal stem cells (BMSCs) and autologous platelet-rich plasma (PRP) in CPC scaffold for bone regeneration in mini...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2416
更新日期：2018-02-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.150
更新日期：2009-02-01 00:00:00
abstract：:In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compr...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2938
更新日期：2020-01-01 00:00:00
abstract：:Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bo...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3124
更新日期：2020-11-01 00:00:00
abstract：:Many studies have highlighted the role of silicon in human bone formation and maintenance. Silicon, in fact, is considered to nucleate the precipitation of hydroxyapatite and to reduce the bone resorption. For this reason, we have combined silk fibroin (SF) with silicon-releasing diatom particles (DPs), as potential m...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2373
更新日期：2018-01-01 00:00:00
abstract：:Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues an...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2092
更新日期：2017-07-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.1973
更新日期：2015-09-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3094
更新日期：2020-09-01 00:00:00
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journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.1923
更新日期：2016-01-01 00:00:00
abstract：:'Off-the-shelf' tissue-engineered skin alternatives for epidermal and dermal skin layers are available; however, no such alternative for the subdermal fat layer exists. Without this well-vascularized layer, skin graft take is variable and grafts may have reduced mobility, contracture and contour defects. In this study...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2025
更新日期：2017-04-01 00:00:00
abstract：:Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ three-dimensional (3D) printing is an exciting and innovative biofabrication technology that enables the surgeon to deliver tissue-engineering techniques at the time and location of need. We ha...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2476
更新日期：2018-03-01 00:00:00
abstract：:Cartilage defects heal imperfectly and osteoarthritic changes develop frequently as a result. Although the existence of specific behaviours of chondrocytes derived from various depth-related zones in vitro has been known for over 20 years, only a relatively small body of in vitro studies has been performed with zonal ...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章,评审
doi：10.1002/term.1638
更新日期：2015-06-01 00:00:00
abstract：BACKGROUND:According to experimental studies, mesh implants may reduce in size up to 50.8% during their integration into soft tissues. This results in impaired mobility of the anterior abdominal wall and hernia recurrences, as well as affects patients' quality of life. Due to unsatisfactory radiographic contrast of pol...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.3029
更新日期：2020-05-01 00:00:00
abstract：:Developing and regenerating neurites respond to a variety of biophysical and biochemical cues in their micro-environment to reach target cells and establish appropriate synapses. Defining the hierarchal relationship of both types of cues to direct neurite growth carries broad significance for neural development, regen...
journal_title：Journal of tissue engineering and regenerative medicine
pub_type： 杂志文章
doi：10.1002/term.2527
更新日期：2018-03-01 00:00:00
abstract：:Whole body vibration (WBV) is a very widespread mechanical stimulus used in physical therapy, rehabilitation and fitness centres. It has been demonstrated that vibration induces improvements in muscular strength and performance and increases bone density. We investigated the effects of low-amplitude, high frequency vi...
journal_title：Journal of tissue engineering and regenerative medicine
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doi：10.1002/term.1533
更新日期：2014-05-01 00:00:00
