Human osteoarthritic chondrons outnumber patient- and joint-matched chondrocytes in hydrogel culture-Future application in autologous cell-based OA cartilage repair?

Abstract:

:Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA chondrons may outperform OA chondrocytes. We compared patient- and joint surface-matched human OA chondrons with OA chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, Collagen II synthesis (CPII) and degradation (C2C), and sulfated glycosaminoglycan. Ex vivo, mRNA expression was comparable. Over time, significant differences in survival led to 3.4-fold higher OA chondron numbers in hydrogels after 2 weeks (p = .002). Significantly, more enzymatically active GAPDH protein indicated higher metabolic activity. The number of cultures that expressed mRNA for Collagen Types I and VI, COMP, aggrecan, VEGF, TGF-β1, and FGF-2 (but not Collagen Types II and X) was different, resulting in a 3.5-fold higher number of expression-positive OA chondron cultures (p < .05). Measuring CPII and C2C per hydrogel, OA chondron hydrogels synthesized more than they degraded Collagen Type II, the opposite was true for OA chondrocytes. Per cell, OA chondrons but not OA chondrocytes displayed more synthesis than degradation. Thus, OA chondrons displayed superior biosynthesis and mRNA expression of tissue engineering and phenotype-relevant genes. Moreover, human OA chondrons displayed a significant survival advantage in hydrogel culture, whose presence, drastic extent, and timescale was novel and is clinically significant. Collectively, these data highlight the high potential of human OA chondrons for OA ACI, as they would outnumber and, thus, surpass OA chondrocytes.

journal_name

J Tissue Eng Regen Med

authors

Rothdiener M,Uynuk-Ool T,Südkamp N,Aurich M,Grodzinsky AJ,Kurz B,Rolauffs B

doi

10.1002/term.2516

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

e1206-e1220

issue

2

eissn

1932-6254

issn

1932-7005

journal_volume

12

pub_type

杂志文章
  • Optimal timing and frequency of bone marrow soup therapy for functional restoration of salivary glands injured by single-dose or fractionated irradiation.

    abstract::Injections of bone marrow (BM) cell extract, known as 'BM soup', were previously reported to mitigate ionizing radiation (IR) injury to salivary glands (SGs). However, the optimal starting time and frequency to maintain BM soup therapeutic efficacy remains unknown. This study tested the optimal starting time and frequ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2513

    authors: Fang D,Shang S,Liu Y,Bakkar M,Sumita Y,Seuntjens J,Tran SD

    更新日期:2018-02-01 00:00:00

  • Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.

    abstract::The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair. A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprot...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2403

    authors: Chevrier A,Darras V,Picard G,Nelea M,Veilleux D,Lavertu M,Hoemann CD,Buschman MD

    更新日期:2018-01-01 00:00:00

  • Rapid treatment of full-thickness skin loss using ovine tendon collagen type I scaffold with skin cells.

    abstract::The full-thickness skin wound is a common skin complication affecting millions of people worldwide. Delayed treatment of this condition causes the loss of skin function and integrity that could lead to the development of chronic wounds or even death. This study was aimed to develop a rapid wound treatment modality usi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2842

    authors: Mh Busra F,Rajab NF,Tabata Y,Saim AB,B H Idrus R,Chowdhury SR

    更新日期:2019-05-01 00:00:00

  • Biodegrading highly porous elastomeric graft regenerates muscular and innervated carotid artery-Comparative study with vein graft.

    abstract::This study aims to investigate the superiorities of fast degrading elastomeric poly(glycerol sebacate) (PGS)/polycaprolactone (PCL) grafts over autologous vein grafts in the reconstruction of carotid artery, thus providing more suitable vascular grafts for carotid artery replacement. We fabricated small arterial graft...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2856

    authors: Yang X,Gao Z,Liu H,Wu W

    更新日期:2019-07-01 00:00:00

  • Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery.

    abstract::Postoperative mucosal regeneration of the middle ear cavity and the mastoid cavity is of great importance after middle ear surgery. However, the epithelialization of the mucosa in the middle ear is retarded because chronic inflammation without epithelialization aggravates gas exchange and clinical function. These envi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2012

    authors: Hama T,Yamamoto K,Yaguchi Y,Murakami D,Sasaki H,Yamato M,Okano T,Kojima H

    更新日期:2017-04-01 00:00:00

  • Kartogenin with PRP promotes the formation of fibrocartilage zone in the tendon-bone interface.

    abstract::Treatment of tendon-bone junction injuries is a challenge because tendon-bone interface often heals poorly and the fibrocartilage zone, which reduces stress concentration, at the interface is not formed. In this study, we used a compound called kartogenin (KGN) with platelet-rich plasma (PRP) to induce the formation o...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2258

    authors: Zhou Y,Zhang J,Yang J,Narava M,Zhao G,Yuan T,Wu H,Zheng N,Hogan MV,Wang JH

    更新日期:2017-12-01 00:00:00

  • PGA-associated heterotopic chondrocyte cocultures: implications of nasoseptal and auricular chondrocytes in articular cartilage repair.

    abstract::The availability of autologous articular chondrocytes remains a limiting issue in matrix assisted autologous chondrocyte transplantation. Non-articular heterotopic chondrocytes could be an alternative autologous cell source. The aims of this study were to establish heterotopic chondrocyte cocultures to analyze cell-ce...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.496

    authors: El Sayed K,Marzahn U,John T,Hoyer M,Zreiqat H,Witthuhn A,Kohl B,Haisch A,Schulze-Tanzil G

    更新日期:2013-01-01 00:00:00

  • Coating bone-like apatite onto organic substrates using solutions mimicking body fluid.

    abstract::Bone-like apatite is a carbonated hydroxyapatite with a small crystallite and low crystallinity. The formation of a layer consisting of bone-like apatite is an essential condition for bioactive materials to achieve direct bonding with living bone. A bone-like apatite layer can be formed on the surface of organic subst...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.3

    authors: Ohtsuki C,Kamitakahara M,Miyazaki T

    更新日期:2007-01-01 00:00:00

  • Label-free relative quantification of secreted proteins as a non-invasive method for the quality control of chondrogenesis in bioengineered substitutes for cartilage repair.

    abstract::Cartilage tissue engineering is making progress, but the competing available strategies still leave room for improvement and consensual overviews regarding the best combinations of scaffolds and cell sources are limited by the capacity to compare them directly. In addition, because most strategies involve autologous c...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2454

    authors: Henrionnet C,Gillet P,Mainard D,Vincourt JB,Pinzano A

    更新日期:2018-03-01 00:00:00

  • Engineered neural tissue with Schwann cell differentiated human dental pulp stem cells: potential for peripheral nerve repair?

    abstract::Despite the spontaneous regenerative capacity of the peripheral nervous system, large gap peripheral nerve injuries (PNIs) require bridging strategies. The limitations and suboptimal results obtained with autografts or hollow nerve conduits in the clinic urge the need for alternative treatments. Recently, we have desc...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2249

    authors: Sanen K,Martens W,Georgiou M,Ameloot M,Lambrichts I,Phillips J

    更新日期:2017-12-01 00:00:00

  • EDTA enhances high-throughput two-dimensional bioprinting by inhibiting salt scaling and cell aggregation at the nozzle surface.

    abstract::Tissue-engineering strategies may be employed in the development of in vitro breast tissue models for use in testing regimens of drug therapies and vaccines. The physical and chemical interactions that occur among cells and extracellular matrix components can also be elucidated with these models to gain an understandi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.162

    authors: Parzel CA,Pepper ME,Burg T,Groff RE,Burg KJ

    更新日期:2009-06-01 00:00:00

  • Hybrid PGS-PCL microfibrous scaffolds with improved mechanical and biological properties.

    abstract::Poly(glycerol sebacate) (PGS) is a biodegradable elastomer that has generated great interest as a scaffold material due to its desirable mechanical properties. However, the use of PGS in tissue engineering is limited by difficulties in casting micro- and nanofibrous structures, due to high temperatures and vacuum requ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.313

    authors: Sant S,Hwang CM,Lee SH,Khademhosseini A

    更新日期:2011-04-01 00:00:00

  • Improved physical and osteoinductive properties of demineralized bone matrix by gelatin methacryloyl formulation.

    abstract::The demineralized bone matrix (DBM) is the most widely used bone allograft, which is obtained by removing the mineral component of bone, leading to exposure of the proteins responsible for osteoinduction. For clinical use, DBM shall be formulated with a carrier that provides consistency and improves its osteoinduction...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.3012

    authors: Ramis JM,Blasco-Ferrer M,Calvo J,Villa O,Cladera MM,Corbillo C,Gayà A,Monjo M

    更新日期:2020-03-01 00:00:00

  • Cyclic tension promotes fibroblastic differentiation of human MSCs cultured on collagen-fibre scaffolds.

    abstract::Mesenchymal stem cells (MSCs) have been suggested as a potential cell source for tendon/ligament tissue engineering. Extrinsic cues, such as the chemical and physical properties of scaffolds, as well as external forces, play an important role in fibroblastic differentiation of these cells. In this study, we employed a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1880

    authors: Qiu Y,Lei J,Koob TJ,Temenoff JS

    更新日期:2016-12-01 00:00:00

  • Mesenchymal stem cells: Cell therapy and regeneration potential.

    abstract::Rapid advances in the isolation of multipotent progenitor cells, routinely called mesenchymal stromal/stem cells (MSCs), from various human tissues and organs have provided impetus to the field of cell therapy and regenerative medicine. The most widely studied sources of MSCs include bone marrow, adipose, muscle, peri...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2914

    authors: Brown C,McKee C,Bakshi S,Walker K,Hakman E,Halassy S,Svinarich D,Dodds R,Govind CK,Chaudhry GR

    更新日期:2019-09-01 00:00:00

  • Prevascularization of natural nanofibrous extracellular matrix for engineering completely biological three-dimensional prevascularized tissues for diverse applications.

    abstract::Self-sustainability after implantation is one of the critical obstacles facing large engineered tissues. A preformed functional vascular network provides an effective solution for solving the mass transportation problem. With the support of mural cells, endothelial cells (ECs) can form microvessels within engineered t...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2512

    authors: Zhang L,Qian Z,Tahtinen M,Qi S,Zhao F

    更新日期:2018-03-01 00:00:00

  • In vitro biocompatibility of 45S5 Bioglass-derived glass-ceramic scaffolds coated with poly(3-hydroxybutyrate).

    abstract::The aim of this work was to study the in vitro biocompatibility of glass-ceramic scaffolds based on 45S5 Bioglass, using a human osteosarcoma cell line (HOS-TE85). The highly porous scaffolds were produced by the foam replication technique. Two different types of scaffolds with different porosities were analysed. They...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.150

    authors: Bretcanu O,Misra S,Roy I,Renghini C,Fiori F,Boccaccini AR,Salih V

    更新日期:2009-02-01 00:00:00

  • Posterior talar process as a suitable cell source for treatment of cartilage and osteochondral defects of the talus.

    abstract::Osteochondral defects of the ankle are common lesions affecting the talar cartilage and subchondral bone. Current treatments include cell-based therapies but are frequently associated with donor-site morbidity. Our objective is to characterize the posterior process of the talus (SP) and the os trigonum (OT) tissues an...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2092

    authors: Correia SI,Silva-Correia J,Pereira H,Canadas RF,da Silva Morais A,Frias AM,Sousa RA,van Dijk CN,Espregueira-Mendes J,Reis RL,Oliveira JM

    更新日期:2017-07-01 00:00:00

  • Testosterone propionate can promote effects of acellular nerve allograft-seeded bone marrow mesenchymal stem cells on repairing canine sciatic nerve.

    abstract::Peripheral human nerves fail to regenerate across long tube implants (>2 cm), and tissue-engineered nerve grafts represent a promising treatment alternative. The present study aims to investigate the testosterone propionate (TP) repair effect of acellular nerve allograft (ANA) seeded with allogeneic bone marrow mesenc...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2922

    authors: Chen C,Tian Y,Wang J,Zhang X,Nan L,Dai P,Gao Y,Zheng S,Liu W,Zhang Y

    更新日期:2019-09-01 00:00:00

  • Differential expression of GAP-43 and neurofilament during peripheral nerve regeneration through bio-artificial conduits.

    abstract::Nerve conduits are promising alternatives for repairing nerve gaps; they provide a close microenvironment that supports nerve regeneration. In this sense, histological analysis of axonal growth is a determinant to achieve successful nerve regeneration. To evaluate this process, the most-used immunohistochemical marker...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1949

    authors: Carriel V,Garzón I,Campos A,Cornelissen M,Alaminos M

    更新日期:2017-02-01 00:00:00

  • Bone regeneration by means of a three-dimensional printed scaffold in a rat cranial defect.

    abstract::Recently, computer-designed three-dimensional (3D) printing techniques have emerged as an active research area with almost unlimited possibilities. In this study, we used a computer-designed 3D scaffold to drive new bone formation in a bone defect. Poly-L-lactide (PLLA) and bioactive β-tricalcium phosphate (TCP) were ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2532

    authors: Kwon DY,Park JH,Jang SH,Park JY,Jang JW,Min BH,Kim WD,Lee HB,Lee J,Kim MS

    更新日期:2018-02-01 00:00:00

  • Thymosin β4 has a major role in dermal burn wound healing that involves actin cytoskeletal remodelling via heat-shock protein 70.

    abstract::Rapid vascular remodelling of damaged dermal tissue is required to heal burn wounds. Thymosin β4 (Tβ4) is a growth factor that has been shown to promote angiogenesis and dermal wound repair. However, the underlying mechanisms based on Tβ4 function have not yet been fully investigated. In the present study, we investig...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2028

    authors: Kim S,Kwon J

    更新日期:2017-04-01 00:00:00

  • Rapid vascularization of starch-poly(caprolactone) in vivo by outgrowth endothelial cells in co-culture with primary osteoblasts.

    abstract::The successful integration of in vitro-generated tissues is dependent on adequate vascularization in vivo. Human outgrowth endothelial cells (OECs) isolated from the mononuclear cell fraction of peripheral blood represent a potent population of circulating endothelial progenitors that could provide a cell source for r...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.373

    authors: Ghanaati S,Fuchs S,Webber MJ,Orth C,Barbeck M,Gomes ME,Reis RL,Kirkpatrick CJ

    更新日期:2011-06-01 00:00:00

  • Cranial reconstruction using allogeneic mesenchymal stromal cells: A phase 1 first-in-human trial.

    abstract::Cranioplasty is necessary for patients that have undergone craniectomy following trauma, stroke or other causes of elevated intracranial pressure. This study assessed the effectiveness of treating cranial defects with allogeneic mesenchymal stromal cells (MSC) on a ceramic carrier and polymer scaffold, to produce viab...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type:

    doi:10.1002/term.2459

    authors: Morrison DA,Kop AM,Nilasaroya A,Sturm M,Shaw K,Honeybul S

    更新日期:2018-02-01 00:00:00

  • Subnormothermic short-term cultivation improves the vascularization capacity of adipose tissue-derived microvascular fragments.

    abstract::Adipose tissue-derived microvascular fragments (ad-MVFs) are promising vascularization units for tissue engineering. In this study, we analysed the effects of normothermic (37°C) and subnormothermic (20°C) short-term cultivation on their viability and network forming capacity. Ad-MVFs from green fluorescent protein (G...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2774

    authors: Laschke MW,Heß A,Scheuer C,Karschnia P,Menger MD

    更新日期:2019-02-01 00:00:00

  • Capturing the stem cell paracrine effect using heparin-presenting nanofibres to treat cardiovascular diseases.

    abstract::The mechanism for stem cell-mediated improvement following acute myocardial infarction has been actively debated. We support hypotheses that the stem cell effect is primarily paracrine factor-linked. We used a heparin-presenting injectable nanofibre network to bind and deliver paracrine factors derived from hypoxic co...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.273

    authors: Webber MJ,Han X,Murthy SN,Rajangam K,Stupp SI,Lomasney JW

    更新日期:2010-12-01 00:00:00

  • Cellular versus acellular matrix devices in the treatment of diabetic foot ulcers: Interim results of a comparative efficacy randomized controlled trial.

    abstract::Healing of diabetic foot ulcers is a major challenge. Despite adhering to optimal standard of care (SOC), less than 30% of wounds heal after 20 weeks. Advanced cellular tissue-based products have shown better healing over SOC, albeit with great cost and modest improvement. We hypothesized no difference in healing effe...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,随机对照试验

    doi:10.1002/term.2884

    authors: Tchanque-Fossuo CN,Dahle SE,Lev-Tov H,West KIM,Li CS,Rocke DM,Isseroff RR

    更新日期:2019-08-01 00:00:00

  • Low-amplitude high frequency vibration down-regulates myostatin and atrogin-1 expression, two components of the atrophy pathway in muscle cells.

    abstract::Whole body vibration (WBV) is a very widespread mechanical stimulus used in physical therapy, rehabilitation and fitness centres. It has been demonstrated that vibration induces improvements in muscular strength and performance and increases bone density. We investigated the effects of low-amplitude, high frequency vi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1533

    authors: Ceccarelli G,Benedetti L,Galli D,Prè D,Silvani G,Crosetto N,Magenes G,Cusella De Angelis MG

    更新日期:2014-05-01 00:00:00

  • Dendrimers: Breaking the paradigm of current musculoskeletal autoimmune therapies.

    abstract::The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2597

    authors: Rodrigues DB,Oliveira JM,Santos TC,Reis RL

    更新日期:2018-03-01 00:00:00

  • Phenotype-based selection of bone marrow mesenchymal stem cell-derived smooth muscle cells for elastic matrix regenerative repair in abdominal aortic aneurysms.

    abstract::Chronic proteolytic disruption of elastic fibres within the abdominal aortic wall results in wall vessel expansion to form rupture-prone abdominal aortic aneurysms (AAA). Arresting AAA growth is not possible as adult vascular smooth muscle cells (SMCs) poorly auto-regenerate and repair elastic fibres. Thus, there is a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2349

    authors: Swaminathan G,Stoilov I,Broekelmann T,Mecham R,Ramamurthi A

    更新日期:2018-01-01 00:00:00