Chondrogenic differentiation of human bone marrow mesenchymal stem cells in chitosan-based scaffolds using a flow-perfusion bioreactor.

Abstract:

:Native articular cartilage is subjected to synovial fluid flow during normal joint function. Thus, it is believed that the morphogenesis of articular cartilage may be positively regulated by the application of similar stimulation in vitro. In the present study, the effect of fluid flow over the chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) was investigated. We intended to find out whether the shear stress caused by perfusion of the medium through the constructs was capable of augmenting the differentiation process. Human BMSCs were isolated from bone marrow aspirates and were characterized by flow cytometry. After expansion, hBM-MSCs were seeded statically onto fibre mesh scaffolds, consisting of a blend of 50:50 chitosan:poly(butylene terephthalate adipate) (CPBTA). Constructs were cultured in a flow-perfusion bioreactor for 28 days, using complete medium for chondrogenesis supplemented by TGFβ3. An enhanced ECM deposition and collagen type II production was observed in the bioreactor samples when compared to the static controls. Moreover, it was observed that hBM-MSCs, in static cultures, take longer to differentiate. ECM accumulation in these samples is lower than in the bioreactor sections, and there is a significant difference in the expression of collagen type I. We found that the flow-induced shear stress has a beneficial effect on the chondrogenic differentiation of hMSCs.

journal_name

J Tissue Eng Regen Med

authors

Alves da Silva ML,Martins A,Costa-Pinto AR,Correlo VM,Sol P,Bhattacharya M,Faria S,Reis RL,Neves NM

doi

10.1002/term.372

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

722-32

issue

9

eissn

1932-6254

issn

1932-7005

journal_volume

5

pub_type

杂志文章
  • Asymmetrical seeding of MSCs into fibrin-poly(ester-urethane) scaffolds and its effect on mechanically induced chondrogenesis.

    abstract::Mesenchymal stem cells (MSCs) are currently being investigated as candidate cells for regenerative medicine approaches for the repair of damaged articular cartilage. For these cells to be used clinically, it is important to understand how they will react to the complex loading environment of a joint in vivo. In additi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2194

    authors: Gardner OFW,Musumeci G,Neumann AJ,Eglin D,Archer CW,Alini M,Stoddart MJ

    更新日期:2017-10-01 00:00:00

  • Three-dimensional 10% cyclic strain reduces bovine aortic endothelial cell angiogenic sprout length and augments tubulogenesis in tubular fibrin hydrogels.

    abstract::The development of a functional microvasculature is critical to the long-term survival of implanted tissue-engineered constructs. Dynamic culture conditions have been shown to significantly modulate phenotypic characteristics and stimulate proliferation of cells within hydrogel-based tissue engineered blood vessels. A...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.323

    authors: Gassman AA,Kuprys T,Ucuzian AA,Brey E,Matsumura A,Pang Y,Larson J,Greisler HP

    更新日期:2011-05-01 00:00:00

  • Biosilicate® and low-level laser therapy improve bone repair in osteoporotic rats.

    abstract::The aim of this study was to investigate the effects of a novel bioactive material (Biosilicate®) and low-level laser therapy (LLLT) on bone fracture consolidation in osteoporotic rats. Forty female Wistar rats were submitted to ovariectomy (OVX) to induce osteopenia. Eight weeks after surgery, the animals were random...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.309

    authors: Bossini PS,Rennó AC,Ribeiro DA,Fangel R,Peitl O,Zanotto ED,Parizotto NA

    更新日期:2011-03-01 00:00:00

  • Mesenchymal stem cells: Cell therapy and regeneration potential.

    abstract::Rapid advances in the isolation of multipotent progenitor cells, routinely called mesenchymal stromal/stem cells (MSCs), from various human tissues and organs have provided impetus to the field of cell therapy and regenerative medicine. The most widely studied sources of MSCs include bone marrow, adipose, muscle, peri...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章,评审

    doi:10.1002/term.2914

    authors: Brown C,McKee C,Bakshi S,Walker K,Hakman E,Halassy S,Svinarich D,Dodds R,Govind CK,Chaudhry GR

    更新日期:2019-09-01 00:00:00

  • 3D ingrowth of bovine articular chondrocytes in biodegradable cryogel scaffolds for cartilage tissue engineering.

    abstract::A feasibility study was undertaken to examine the potential of biodegradable HEMA-lactate-dextran (HEMA-LLA-D)-based cryogels as scaffolds for cartilage tissue engineering. This was a preliminary in vitro study giving essential information on the biocompatibility of cryogels with cartilage cells. HEMA-lactate (HEMA-LL...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.375

    authors: Bölgen N,Yang Y,Korkusuz P,Güzel E,El Haj AJ,Pişkin E

    更新日期:2011-11-01 00:00:00

  • EDTA enhances high-throughput two-dimensional bioprinting by inhibiting salt scaling and cell aggregation at the nozzle surface.

    abstract::Tissue-engineering strategies may be employed in the development of in vitro breast tissue models for use in testing regimens of drug therapies and vaccines. The physical and chemical interactions that occur among cells and extracellular matrix components can also be elucidated with these models to gain an understandi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.162

    authors: Parzel CA,Pepper ME,Burg T,Groff RE,Burg KJ

    更新日期:2009-06-01 00:00:00

  • Human osteoarthritic chondrons outnumber patient- and joint-matched chondrocytes in hydrogel culture-Future application in autologous cell-based OA cartilage repair?

    abstract::Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA chondrons may outperform OA...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2516

    authors: Rothdiener M,Uynuk-Ool T,Südkamp N,Aurich M,Grodzinsky AJ,Kurz B,Rolauffs B

    更新日期:2018-02-01 00:00:00

  • Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages.

    abstract::Prudent choices of cell sources and biomaterials, as well as meticulous cultivation of the tissue microenvironment, are essential to improving outcomes of tissue engineering treatments. With the goal of providing a high-quality alternative for bone and cartilage tissue engineering, we investigated the capability of bo...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.3124

    authors: Baracho Trindade Hill A,Speri Alves AA,da Silva Nunes Barreto R,Fernandes Bressan F,Miglino MA,Mansano Garcia J

    更新日期:2020-11-01 00:00:00

  • Virus immobilization on biomaterial scaffolds through biotin-avidin interaction for improving bone regeneration.

    abstract::To spatially control therapeutic gene delivery for potential tissue engineering applications, a biotin-avidin interaction strategy was applied to immobilize viral vectors on biomaterial scaffolds. Both adenoviral vectors and gelatin sponges were biotinylated and avidin was applied to link them in a virus-biotin-avidin...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1774

    authors: Hu WW,Wang Z,Krebsbach PH

    更新日期:2016-02-01 00:00:00

  • In vivo analysis of vascularization and biocompatibility of electrospun polycaprolactone fibre mats in the rat femur chamber.

    abstract::In orthopaedic medicine, connective tissues are often affected by traumatic or degenerative injuries, and surgical intervention is required. Rotator cuff tears are a common cause of shoulder pain and disability among adults. The development of graft materials for bridging the gap between tendon and bone after chronic ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2868

    authors: Gniesmer S,Brehm R,Hoffmann A,de Cassan D,Menzel H,Hoheisel AL,Glasmacher B,Willbold E,Reifenrath J,Wellmann M,Ludwig N,Tavassol F,Zimmerer R,Gellrich NC,Kampmann A

    更新日期:2019-07-01 00:00:00

  • Numerical assessment on the effective mechanical stimuli for matrix-associated metabolism in chondrocyte-seeded constructs.

    abstract::The self-regeneration capacity of articular cartilage is limited, due to its avascular and aneural nature. Loaded explants and cell cultures demonstrated that chondrocyte metabolism can be regulated via physiologic loading. However, the explicit ranges of mechanical stimuli that correspond to favourable metabolic resp...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.307

    authors: Tasci A,Ferguson SJ,Büchler P

    更新日期:2011-03-01 00:00:00

  • Bone regeneration in minipigs via calcium phosphate cement scaffold delivering autologous bone marrow mesenchymal stem cells and platelet-rich plasma.

    abstract::Macroporous calcium phosphate cement (CPC) with stem cell seeding is promising for bone regeneration. The objective of this study was to investigate the effects of co-delivering autologous bone marrow mesenchymal stem cells (BMSCs) and autologous platelet-rich plasma (PRP) in CPC scaffold for bone regeneration in mini...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2416

    authors: Qiu G,Shi Z,Xu HHK,Yang B,Weir MD,Li G,Song Y,Wang J,Hu K,Wang P,Zhao L

    更新日期:2018-02-01 00:00:00

  • Delivering stem cells to the healthy heart on biological sutures: effects on regional mechanical function.

    abstract::Current cardiac cell therapies cannot effectively target and retain cells in a specific area of the heart. Cell-seeded biological sutures were previously developed to overcome this limitation, demonstrating targeted delivery with > 60% cell retention. In this study, both cell-seeded and non-seeded fibrin-based biologi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1904

    authors: Tao ZW,Favreau JT,Guyette JP,Hansen KJ,Lessard J,Burford E,Pins GD,Gaudette GR

    更新日期:2017-01-01 00:00:00

  • Biological performance of cell-encapsulated methacrylated gellan gum-based hydrogels for nucleus pulposus regeneration.

    abstract::Limitations of current treatments for intervertebral disc (IVD) degeneration have promoted interest in the development of tissue-engineering approaches. Injectable hydrogels loaded with cells can be used as a substitute material for the inner IVD part, the nucleus pulposus (NP), and provide an opportunity for minimall...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1959

    authors: Tsaryk R,Silva-Correia J,Oliveira JM,Unger RE,Landes C,Brochhausen C,Ghanaati S,Reis RL,Kirkpatrick CJ

    更新日期:2017-03-01 00:00:00

  • In vitro two-dimensional and three-dimensional tenocyte culture for tendon tissue engineering.

    abstract::In order to examine the differentiation potential of the tenocytes expanded in our defined culture medium (reported previously) and the effect of sequential combination of the two culture conditions on human tenocytes, a two-dimensional and three-dimensional experimental approach was used. Human tenocytes were sequent...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1791

    authors: Qiu Y,Wang X,Zhang Y,Carr AJ,Zhu L,Xia Z,Sabokbar A

    更新日期:2016-03-01 00:00:00

  • Three-dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells.

    abstract::To establish an ex vivo expansion method of haematopoietic progenitor cells (HPCs) and erythroid cells, three-dimensional (3D) cultures of mouse bone marrow cells were performed, employing a porous polyvinyl formal (PVF) resin as a scaffold. In these cultures, the effects of oxygen concentration and co-cultures with s...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.295

    authors: Miyoshi H,Murao M,Ohshima N,Tun T

    更新日期:2011-02-01 00:00:00

  • Bone regeneration by means of a three-dimensional printed scaffold in a rat cranial defect.

    abstract::Recently, computer-designed three-dimensional (3D) printing techniques have emerged as an active research area with almost unlimited possibilities. In this study, we used a computer-designed 3D scaffold to drive new bone formation in a bone defect. Poly-L-lactide (PLLA) and bioactive β-tricalcium phosphate (TCP) were ...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2532

    authors: Kwon DY,Park JH,Jang SH,Park JY,Jang JW,Min BH,Kim WD,Lee HB,Lee J,Kim MS

    更新日期:2018-02-01 00:00:00

  • Morphological transformation of hBMSC from 2D monolayer to 3D microtissue on low-crystallinity SF-PCL patch with promotion of cardiomyogenesis.

    abstract::The effects of the stiffness of substrates on the cell behaviours of human bone marrow-derived mesenchymal stem cells (hBMSC) have been investigated, but the effects of the secondary structures of proteins in the substrates on the morphological transformation and differentiation of hBMSC have yet been elucidated. To i...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2616

    authors: Lo HY,Huang AL,Lee PC,Chung TW,Wang SS

    更新日期:2018-04-01 00:00:00

  • An in vitro expansion score for tissue-engineering applications with human bone marrow-derived mesenchymal stem cells.

    abstract::Human bone marrow-derived mesenchymal stem cells (MSCs) have limited growth potential in vitro and cease to divide due to replicative senescence, which from a tissue-engineering perspective has practical implications, such as defining the correct starting points for differentiation and transplantation. Time spent in c...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1734

    authors: Bertolo A,Mehr M,Janner-Jametti T,Graumann U,Aebli N,Baur M,Ferguson SJ,Stoyanov JV

    更新日期:2016-02-01 00:00:00

  • Hepatogenic engineering from human bone marrow mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture.

    abstract::Bone marrow mesenchymal stem cells (MSCs) are promising candidates for cell therapy and tissue engineering. We used mesenchymal stem cells from human bone marrow (hMSCs) as the seeding cells to investigate the potential of hepatocytic differentiation of hMSCs in porous polylactic glycolic acid (PLGA) scaffolds under p...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.393

    authors: Wang J,Zong C,Shi D,Wang W,Shen D,Liu L,Tong X,Zheng Q,Gao C

    更新日期:2012-01-01 00:00:00

  • A biological approach to assembling tissue modules through endothelial capillary network formation.

    abstract::To create functional tissues having complex structures, bottom-up approaches to assembling small tissue modules into larger constructs have been emerging. Most of these approaches are based on chemical reactions or physical interactions at the interface between tissue modules. Here we report a biological assembly appr...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2008

    authors: Riesberg JJ,Shen W

    更新日期:2015-09-01 00:00:00

  • Epidermal-like architecture obtained from equine keratinocytes in three-dimensional cultures.

    abstract::Despite the high prevalence of skin conditions in the horse, there is a dearth of literature on the culture and biology of equine skin cells, and this is partially attributable to the lack of suitable in vitro skin models. The objective of this study was to develop a three-dimensional (3D) culture system that would su...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1788

    authors: Sharma R,Barakzai SZ,Taylor SE,Donadeu FX

    更新日期:2016-08-01 00:00:00

  • In vitro biocompatibility of 45S5 Bioglass-derived glass-ceramic scaffolds coated with poly(3-hydroxybutyrate).

    abstract::The aim of this work was to study the in vitro biocompatibility of glass-ceramic scaffolds based on 45S5 Bioglass, using a human osteosarcoma cell line (HOS-TE85). The highly porous scaffolds were produced by the foam replication technique. Two different types of scaffolds with different porosities were analysed. They...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.150

    authors: Bretcanu O,Misra S,Roy I,Renghini C,Fiori F,Boccaccini AR,Salih V

    更新日期:2009-02-01 00:00:00

  • Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery.

    abstract::Postoperative mucosal regeneration of the middle ear cavity and the mastoid cavity is of great importance after middle ear surgery. However, the epithelialization of the mucosa in the middle ear is retarded because chronic inflammation without epithelialization aggravates gas exchange and clinical function. These envi...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2012

    authors: Hama T,Yamamoto K,Yaguchi Y,Murakami D,Sasaki H,Yamato M,Okano T,Kojima H

    更新日期:2017-04-01 00:00:00

  • Phenotype-based selection of bone marrow mesenchymal stem cell-derived smooth muscle cells for elastic matrix regenerative repair in abdominal aortic aneurysms.

    abstract::Chronic proteolytic disruption of elastic fibres within the abdominal aortic wall results in wall vessel expansion to form rupture-prone abdominal aortic aneurysms (AAA). Arresting AAA growth is not possible as adult vascular smooth muscle cells (SMCs) poorly auto-regenerate and repair elastic fibres. Thus, there is a...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2349

    authors: Swaminathan G,Stoilov I,Broekelmann T,Mecham R,Ramamurthi A

    更新日期:2018-01-01 00:00:00

  • Effects of designed PLLA and 50:50 PLGA scaffold architectures on bone formation in vivo.

    abstract::Biodegradable porous scaffolds have been investigated as an alternative approach to current metal, ceramic, and polymer bone graft substitutes for lost or damaged bone tissues. Although there have been many studies investigating the effects of scaffold architecture on bone formation, many of these scaffolds were fabri...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.497

    authors: Saito E,Liao EE,Hu WW,Krebsbach PH,Hollister SJ

    更新日期:2013-02-01 00:00:00

  • A papain-induced disc degeneration model for the assessment of thermo-reversible hydrogel-cells therapeutic approach.

    abstract::Nucleus pulposus (NP) regeneration by the application of injectable cell-embedded hydrogels is an appealing approach for tissue engineering. We investigated a thermo-reversible hydrogel (TR-HG), based on a modified polysaccharide with a thermo-reversible polyamide [poly(N-isopropylacrylamide), pNIPAM], which is made t...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.1667

    authors: Malonzo C,Chan SC,Kabiri A,Eglin D,Grad S,Bonél HM,Benneker LM,Gantenbein-Ritter B

    更新日期:2015-12-01 00:00:00

  • Preservation of microvascular integrity and immunomodulatory property of prevascularized human mesenchymal stem cell sheets.

    abstract::Prevascularization is essential to ensure the viability, functionality, and successful integration of tissue-engineered three-dimensional (3D) constructs with surrounding host tissues after transplantation. Human mesenchymal stem cell (hMSC) sheet can be prevascularized by coculturing with endothelial cells (ECs), and...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.3167

    authors: Jia W,Sharma D,He W,Xing Q,Zhao F

    更新日期:2021-01-11 00:00:00

  • Injectable chitosan-platelet-rich plasma implants to promote tissue regeneration: in vitro properties, in vivo residence, degradation, cell recruitment and vascularization.

    abstract::The purpose of this study was to develop freeze-dried chitosan formulations that can be solubilized in platelet-rich plasma (PRP) to form injectable implants for tissue repair. A systematic approach to adjust formulation parameters, including chitosan number average molar mass (Mn ), chitosan concentration and lyoprot...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.2403

    authors: Chevrier A,Darras V,Picard G,Nelea M,Veilleux D,Lavertu M,Hoemann CD,Buschman MD

    更新日期:2018-01-01 00:00:00

  • Controlled collagen crosslinking process in tissue-engineered fibroblast sheets for preventing scar contracture on the surface of lungs.

    abstract::For preventing the scar contracture of host tissue and adjusting the tensile strength of covering cell sheets, a controlled collagen crosslinking step process in the preparation of skin-fibroblast sheets for repairing wound was investigated by using β-aminopropionitrile (BAPN), a collagen crosslinking inhibitor, in th...

    journal_title:Journal of tissue engineering and regenerative medicine

    pub_type: 杂志文章

    doi:10.1002/term.533

    authors: Kanzaki M,Yamato M,Takagi R,Kikkawa T,Isaka T,Okano T,Onuki T

    更新日期:2013-05-01 00:00:00