Abstract:
PURPOSE:Coagulase-negative staphylococci have been reported to be the most frequent cause of bacterial postoperative endophthalmitis. Biofilm formation is the major virulence factor of Staphylococcus epidermidis and is often associated with methicillin resistance. This study aims at evaluating the presence of biofilm-producing and methicillin resistance genes (mecA) in S. epidermidis. METHODS:S. epidermidis isolated from clinically infected sites (group 1) and from normal human conjunctiva (group 2) were studied. All the isolates were tested for their ability to produce biofilm by the conventional Christensen´s method and the presence of mecA by PCR using the 22-mer oligonucleotides as primers. RESULTS:In total 20 isolates from group 1 and 22 from group 2 were studied. Biofilm and mecA were detected in 15 (75 %) and in 14 (70 %) in group 1 as compared to 8 (36.3 %) and 4 (18.2 %) in group 2 (p = 0.016). Simultaneously, biofilm production and presence of mecA genes were observed in 13/20 (65.0 %) in group 1, and 4/22 (18.2 %) in group 2 (p = 0.002). Multi-resistance was observed in 55 % in group 1 and 9 % in group 2 (p = 0.002); 57 % of the biofilm-producing strains was multi-resistant in contrast to none of the non-producing strains. In all multi-resistant strains, biofilm production was seen. CONCLUSIONS:Biofilm formation capacity was widely distributed, particularly among mecA (+) S. epidermidis strains, which also displayed a high diversity of antibiotic resistance profiles.
journal_name
Int Ophthalmoljournal_title
International ophthalmologyauthors
Fariña N,Samudio M,Carpinelli L,Nentwich MM,de Kaspar HMdoi
10.1007/s10792-016-0339-8subject
Has Abstractpub_date
2017-08-01 00:00:00pages
819-825issue
4eissn
0165-5701issn
1573-2630pii
10.1007/s10792-016-0339-8journal_volume
37pub_type
杂志文章abstract:PURPOSE:The purpose of this article was to introduce a novel surgical technique for the management of peripheral Descemet's membrane perforation during deep anterior lamellar keratoplasty (DALK). METHODS:First, a thin stromal patch was prepared either from the anterior stromal lamella cut during DALK or from the anter...
journal_title:International ophthalmology
pub_type: 杂志文章
doi:10.1007/s10792-018-01065-6
更新日期:2019-10-01 00:00:00
abstract::Primary lacrimal sac melanoma is an extremely rare clinical entity with fewer than 25 cases reported in the literature. We present a well-documented case of lacrimal sac melanoma with anterior lacrimal crest infiltration and discuss the clinical, radiological and histopathological features along with surgical challeng...
journal_title:International ophthalmology
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更新日期:2014-02-01 00:00:00
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journal_title:International ophthalmology
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doi:10.1007/s10792-020-01585-0
更新日期:2021-01-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章,评审
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更新日期:2012-08-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
abstract::To report a unusual case of an isolated traumatic stellated tarso-conjunctival laceration located in the upper left eyelid without eyelid margin involvement and with normal ocular examination. A 19 year-old male wounded by a bokken (wooden katana) while practicing a sham duel had an isolated eyelid tarso-conjunctival ...
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pub_type: 杂志文章,随机对照试验
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更新日期:2019-07-01 00:00:00
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更新日期:1987-02-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章,随机对照试验
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更新日期:2010-10-01 00:00:00
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更新日期:2017-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/a:1014417606396
更新日期:2001-01-01 00:00:00
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更新日期:2011-10-01 00:00:00
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更新日期:2014-04-01 00:00:00
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journal_title:International ophthalmology
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更新日期:2014-06-01 00:00:00
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更新日期:2013-04-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章
doi:10.1023/a:1006055918101
更新日期:1997-01-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章
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journal_title:International ophthalmology
pub_type: 杂志文章,评审
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更新日期:1991-01-01 00:00:00
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journal_title:International ophthalmology
pub_type: 杂志文章
doi:10.1007/BF00130696
更新日期:1981-05-01 00:00:00
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更新日期:2018-02-01 00:00:00