Adaptation to Blue Light in Marine Synechococcus Requires MpeU, an Enzyme with Similarity to Phycoerythrobilin Lyase Isomerases.

Abstract:

:Marine Synechococcus has successfully adapted to environments with different light colors, which likely contributes to this genus being the second most abundant group of microorganisms worldwide. Populations of Synechococcus that grow in deep, blue ocean waters contain large amounts of the blue-light absorbing chromophore phycourobilin (PUB) in their light harvesting complexes (phycobilisomes). Here, we show that all Synechococcus strains adapted to blue light possess a gene called mpeU. MpeU is structurally similar to phycobilin lyases, enzymes that ligate chromophores to phycobiliproteins. Interruption of mpeU caused a reduction in PUB content, impaired phycobilisome assembly and reduced growth rate more strongly in blue than green light. When mpeU was reintroduced in the mpeU mutant background, the mpeU-less phenotype was complemented in terms of PUB content and phycobilisome content. Fluorescence spectra of mpeU mutant cells and purified phycobilisomes revealed red-shifted phycoerythrin emission peaks, likely indicating a defect in chromophore ligation to phycoerythrin-I (PE-I) or phycoerythrin-II (PE-II). Our results suggest that MpeU is a lyase-isomerase that attaches a phycoerythrobilin to a PEI or PEII subunit and isomerizes it to PUB. MpeU is therefore an important determinant in adaptation of Synechococcus spp. to capture photons in blue light environments throughout the world's oceans.

journal_name

Front Microbiol

authors

Mahmoud RM,Sanfilippo JE,Nguyen AA,Strnat JA,Partensky F,Garczarek L,Abo El Kassem N,Kehoe DM,Schluchter WM

doi

10.3389/fmicb.2017.00243

subject

Has Abstract

pub_date

2017-02-21 00:00:00

pages

243

issn

1664-302X

journal_volume

8

pub_type

杂志文章
  • Variability of Gut Microbiota Across the Life Cycle of Grapholita molesta (Lepidoptera: Tortricidae).

    abstract::Grapholita molesta, the oriental fruit moth, is a serious global pest of many Rosaceae fruit trees. Gut microorganisms play important roles in host nutrition, digestion, detoxification, and resistance to pathogens. However, there are few studies on the microbiota of G. molesta, particularly during metamorphosis. Here,...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01366

    authors: Wang X,Sun S,Yang X,Cheng J,Wei H,Li Z,Michaud JP,Liu X

    更新日期:2020-06-30 00:00:00

  • A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types.

    abstract::A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00476

    authors: Lever MA,Torti A,Eickenbusch P,Michaud AB,Šantl-Temkiv T,Jørgensen BB

    更新日期:2015-05-19 00:00:00

  • A Replisome's journey through the bacterial chromosome.

    abstract::Genome duplication requires the coordinated activity of a multi-component machine, the replisome. In contrast to the background of metabolic diversity across the bacterial domain, the composition and architecture of the bacterial replisome seem to have suffered few changes during evolution. This immutability underline...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00562

    authors: Beattie TR,Reyes-Lamothe R

    更新日期:2015-06-05 00:00:00

  • Estimating HIV-1 Genetic Diversity in Brazil Through Next-Generation Sequencing.

    abstract::Approximately 36.7 million people were living with the human immunodeficiency virus (HIV) at the end of 2016 according to UNAIDS, representing a global prevalence rate of 0.8%. In Brazil, an HIV prevalence of 0.24% has been estimated, which represents approximately 830,000 individuals living with the virus. As a touri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00749

    authors: Alves BM,Siqueira JD,Prellwitz IM,Botelho OM,Da Hora VP,Sanabani S,Recordon-Pinson P,Fleury H,Soares EA,Soares MA

    更新日期:2019-04-09 00:00:00

  • Genomic Analysis of Carbapenem-Resistant Acinetobacter baumannii Isolates Belonging to Major Endemic Clones in South America.

    abstract::Carbapenem-resistant Acinetobacter baumannii (CRAB) are emerging worldwide. In South America, clinical isolates presenting such a phenotype usually do not belong to the globally distributed international clone 2 (IC2). The majority of these isolates are also resistant to multiple other antimicrobials and are often des...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.584603

    authors: Nodari CS,Cayô R,Streling AP,Lei F,Wille J,Almeida MS,de Paula AI,Pignatari ACC,Seifert H,Higgins PG,Gales AC

    更新日期:2020-11-30 00:00:00

  • Update on Pyrin Functions and Mechanisms of Familial Mediterranean Fever.

    abstract::Mutations in the MEFV gene, which encodes the protein named pyrin (also called marenostrin or TRIM20), are associated with the autoinflammatory disease familial Mediterranean fever (FMF). Recent genetic and immunologic studies uncovered novel functions of pyrin and raised several new questions in relation to FMF patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.00456

    authors: Manukyan G,Aminov R

    更新日期:2016-03-31 00:00:00

  • Protection Mechanism of Clostridium butyricum against Salmonella Enteritidis Infection in Broilers.

    abstract::This study was designed to evaluate the protection mechanism of oral administration of Clostridium butyricum against Salmonella enteritidis (SE) colonization in broilers. In the current study, 180 one-day-old healthy Arbor Acres (AA) broilers were meanly grouped into three, with three replicates of 20 birds each. An n...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01523

    authors: Zhao X,Yang J,Wang L,Lin H,Sun S

    更新日期:2017-08-09 00:00:00

  • Comparison of methods for the enumeration of enterohemorrhagic Escherichia coli from veal hides and carcasses.

    abstract::The increased association of enterohemorrhagic Escherichia coli (EHEC) with veal calves has led the United States Department of Agriculture Food Safety and Inspection Service to report results of veal meat contaminated with the Top 7 serogroups separately from beef cattle. However, detection methods that can also prov...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01062

    authors: Luedtke BE,Bosilevac JM

    更新日期:2015-09-29 00:00:00

  • Evaluation of the biodegradation of Alaska North Slope oil in microcosms using the biodegradation model BIOB.

    abstract::We present the details of a numerical model, BIOB that is capable of simulating the biodegradation of oil entrapped in the sediment. The model uses Monod kinetics to simulate the growth of bacteria in the presence of nutrients and the subsequent consumption of hydrocarbons. The model was used to simulate experimental ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2014.00212

    authors: Torlapati J,Boufadel MC

    更新日期:2014-05-14 00:00:00

  • The Impact of ackA, pta, and ackA-pta Mutations on Growth, Gene Expression and Protein Acetylation in Escherichia coli K-12.

    abstract::Acetate is a characteristic by-product of Escherichia coli K-12 growing in batch cultures with glucose, both under aerobic as well as anaerobic conditions. While the reason underlying aerobic acetate production is still under discussion, during anaerobic growth acetate production is important for ATP generation by sub...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00233

    authors: Schütze A,Benndorf D,Püttker S,Kohrs F,Bettenbrock K

    更新日期:2020-02-21 00:00:00

  • Temperate Prophages Increase Bacterial Adhesin Expression and Virulence in an Experimental Model of Endocarditis Due to Staphylococcus aureus From the CC398 Lineage.

    abstract::Until 2007, Staphylococcus aureus from clonal complex 398 (CC398) was exclusively associated with livestock species and companion animals. Recently, several studies described the emergence of S. aureus CC398 as etiologies of severe infections in humans living in an animal-free environment. Recent sequencing efforts sh...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00742

    authors: Laumay F,Corvaglia AR,Diene SM,Girard M,Oechslin F,van der Mee-Marquet N,Entenza JM,François P

    更新日期:2019-04-24 00:00:00

  • Mechanism of Action of Surface Immobilized Antimicrobial Peptides Against Pseudomonas aeruginosa.

    abstract::Bacterial colonization and biofilm development on medical devices can lead to infection. Antimicrobial peptide-coated surfaces may prevent such infections. Melimine and Mel4 are chimeric cationic peptides showing broad-spectrum antimicrobial activity once attached to biomaterials and are highly biocompatible in animal...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.03053

    authors: Yasir M,Dutta D,Hossain KR,Chen R,Ho KKK,Kuppusamy R,Clarke RJ,Kumar N,Willcox MDP

    更新日期:2020-01-22 00:00:00

  • The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members.

    abstract::The Streptococcus bovis/Streptococcus equinus complex (SBSEC) comprises several species inhabiting the animal and human gastrointestinal tract (GIT). They match the pathobiont description, are potential zoonotic agents and technological organisms in fermented foods. SBSEC members are associated with multiple diseases ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.00603

    authors: Jans C,Boleij A

    更新日期:2018-04-10 00:00:00

  • Reconstruction and Analysis of a Genome-Scale Metabolic Model of Ganoderma lucidum for Improved Extracellular Polysaccharide Production.

    abstract::In this study, we reconstructed for the first time a genome-scale metabolic model (GSMM) of Ganoderma lucidum strain CGMCC5.26, termed model iZBM1060, containing 1060 genes, 1202 metabolites, and 1404 reactions. Important findings based on model iZBM1060 and its predictions are as follows: (i) The extracellular polysa...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.03076

    authors: Ma Z,Ye C,Deng W,Xu M,Wang Q,Liu G,Wang F,Liu L,Xu Z,Shi G,Ding Z

    更新日期:2018-12-11 00:00:00

  • Studying Biofilm and Clinical Issues in Orthopedics.

    abstract::The association between biofilm-forming microorganisms and prosthetic joint infection influences all aspect of management including approaches to diagnosis, management and prevention. This article will provide an overview of new anti-biofilm strategies for management of prosthetic joint infection. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2019.00359

    authors: Peel TN

    更新日期:2019-02-26 00:00:00

  • Comparative Functional Genomic Analysis of Two Vibrio Phages Reveals Complex Metabolic Interactions with the Host Cell.

    abstract::Sequencing and annotation was performed for two large double stranded DNA bacteriophages, φGrn1 and φSt2 of the Myoviridae family, considered to be of great interest for phage therapy against Vibrios in aquaculture live feeds. In addition, phage-host metabolic interactions and exploitation was studied by transcript pr...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01807

    authors: Skliros D,Kalatzis PG,Katharios P,Flemetakis E

    更新日期:2016-11-14 00:00:00

  • In Vitro/Vivo Activity of Potential MCR-1 Inhibitor in Combination With Colistin Againsts mcr-1-Positive Klebsiella pneumonia.

    abstract::Carbapenem resistance among strains of the nosocomial pathogen Klebsiella pneumoniae is increasing worldwide, causing serious clinical infections and higher mortality rates. Polymyxins are some of the few "last resort" options for treatment of carbapenem-resistant Enterobacteriaceae, including K. pneumoniae, however, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01615

    authors: Zhou Y,Wang T,Guo Y,Liu S,Wang J,Shen Y,Tang S,Wang Y,Deng X

    更新日期:2018-07-17 00:00:00

  • Species-Level Analysis of Human Gut Microbiota With Metataxonomics.

    abstract::The current understanding of human gut microbial community is mainly limited to taxonomic features at the genus level. Here, we examined the human gut microbial community at the species level by metataxonomics. To achieve this purpose, a high-throughput approach involving operational phylogenetic unit analysis of the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.02029

    authors: Yang J,Pu J,Lu S,Bai X,Wu Y,Jin D,Cheng Y,Zhang G,Zhu W,Luo X,Rosselló-Móra R,Xu J

    更新日期:2020-08-26 00:00:00

  • Antibacterial Effect of Copaifera duckei Dwyer Oleoresin and Its Main Diterpenes against Oral Pathogens and Their Cytotoxic Effect.

    abstract::This study evaluates the antibacterial activity of the Copaifera duckei Dwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00201

    authors: Abrão F,Alves JA,Andrade G,de Oliveira PF,Ambrósio SR,Veneziani RCS,Tavares DC,Bastos JK,Martins CHG

    更新日期:2018-02-21 00:00:00

  • Spatial Heterogeneity and Co-occurrence of Mucosal and Luminal Microbiome across Swine Intestinal Tract.

    abstract::Pigs are one of the most important economic livestock. Gut microbiota is not only critical to the health but also the production efficiency of pigs. Manipulating gut microbiota relies on the full view of gut microbiome and the understanding of drive forces shaping microbial communities. 16s rDNA sequencing was used to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00048

    authors: Zhang L,Wu W,Lee YK,Xie J,Zhang H

    更新日期:2018-01-26 00:00:00

  • Polyelectrolyte Multicomponent Colloidosomes Loaded with Nisin Z for Enhanced Antimicrobial Activity against Foodborne Resistant Pathogens.

    abstract::Food grade micro- or nano-carrier systems (NCS) are being developed to improve the controlled release of antimicrobial agents. To augment the stability of liposomal NCS and to overcome the limitations associated with the use of free bacteriocin (nisin) in the food system, multi-component colloidosomes (MCCS) were deve...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02700

    authors: Niaz T,Shabbir S,Noor T,Abbasi R,Raza ZA,Imran M

    更新日期:2018-01-15 00:00:00

  • Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated From Broiler Carcasses in Belgium.

    abstract::Human campylobacteriosis is the leading food-borne zoonosis in industrialized countries. This study characterized the clonal population structure, antimicrobial resistance profiles and occurrence of antimicrobial resistance determinants of a set of Campylobacter jejuni strains isolated from broiler carcasses in Belgiu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01014

    authors: Elhadidy M,Miller WG,Arguello H,Álvarez-Ordóñez A,Duarte A,Dierick K,Botteldoorn N

    更新日期:2018-05-17 00:00:00

  • Depth Influences Symbiodiniaceae Associations Among Montastraea cavernosa Corals on the Belize Barrier Reef.

    abstract::In Belize, shallow populations (10 and 16 m) of the coral species Montastraea cavernosa from the back reef and reef crest are genetically differentiated from deeper populations on the fore reef and reef wall (25 and 35 m). Like many species of scleractinian corals, M. cavernosa has an obligate symbiosis with dinoflage...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00518

    authors: Eckert RJ,Reaume AM,Sturm AB,Studivan MS,Voss JD

    更新日期:2020-04-09 00:00:00

  • Distinct Expression Levels of ALS, LIP, and SAP Genes in Candida tropicalis with Diverse Virulent Activities.

    abstract::Candia tropicalis is an increasingly important human pathogen, causing nosocomial fungemia among patients with neutropenia or malignancy. However, limited research has been published concerning its pathogenicity. Based on the phenotypes of C. tropicalis in our previous study, we selected nine representative strains wi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01175

    authors: Yu S,Li W,Liu X,Che J,Wu Y,Lu J

    更新日期:2016-07-29 00:00:00

  • Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol.

    abstract::Molasses are sub-products of the sugar industry, rich in sucrose and containing other sugars like raffinose, glucose, and fructose. Alpha-galactosidases (EC. 3.2.1.22) catalyze the hydrolysis of alpha-(1,6) bonds of galactose residues in galacto-oligosaccharides (melibiose, raffinose, and stachyose) and complex galact...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00405

    authors: Álvarez-Cao ME,Cerdán ME,González-Siso MI,Becerra M

    更新日期:2019-03-07 00:00:00

  • The Use of Transposon Insertion Sequencing to Interrogate the Core Functional Genome of the Legume Symbiont Rhizobium leguminosarum.

    abstract::The free-living legume symbiont Rhizobium leguminosarum is of significant economic value because of its ability to provide fixed nitrogen to globally important leguminous food crops, such as peas and lentils. Discovery based research into the genetics and physiology of R. leguminosarum provides the foundational knowle...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01873

    authors: Perry BJ,Akter MS,Yost CK

    更新日期:2016-11-22 00:00:00

  • Ammonia-Oxidizing Archaea Are More Resistant Than Denitrifiers to Seasonal Precipitation Changes in an Acidic Subtropical Forest Soil.

    abstract::Seasonal precipitation changes are increasingly severe in subtropical areas. However, the responses of soil nitrogen (N) cycle and its associated functional microorganisms to such precipitation changes remain unclear. In this study, two projected precipitation patterns were manipulated: intensifying the dry-season dro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01384

    authors: Chen J,Nie Y,Liu W,Wang Z,Shen W

    更新日期:2017-07-24 00:00:00

  • PBHMDA: Path-Based Human Microbe-Disease Association Prediction.

    abstract::With the advance of sequencing technology and microbiology, the microorganisms have been found to be closely related to various important human diseases. The increasing identification of human microbe-disease associations offers important insights into the underlying disease mechanism understanding from the perspectiv...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00233

    authors: Huang ZA,Chen X,Zhu Z,Liu H,Yan GY,You ZH,Wen Z

    更新日期:2017-02-22 00:00:00

  • Evaluation of a Major Surface Antigen of Babesia microti Merozoites as a Vaccine Candidate against Babesia Infection.

    abstract::Babesia species are tick-borne intraerythrocytic protozoa that cause babesiosis in humans worldwide. No vaccine has yet proven effective against Babesia infection. Surface antigens of merozoites are involved in the invasion of erythrocytes by Babesia. Surface antigens may be presented by both babesial sporozoites and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02545

    authors: Man S,Fu Y,Guan Y,Feng M,Qiao K,Li X,Gao H,Cheng X

    更新日期:2017-12-19 00:00:00

  • Fungal ITS1 Deep-Sequencing Strategies to Reconstruct the Composition of a 26-Species Community and Evaluation of the Gut Mycobiota of Healthy Japanese Individuals.

    abstract::The study of mycobiota remains relatively unexplored due to the lack of sufficient available reference strains and databases compared to those of bacterial microbiome studies. Deep sequencing of Internal Transcribed Spacer (ITS) regions is the de facto standard for fungal diversity analysis. However, results are often...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00238

    authors: Motooka D,Fujimoto K,Tanaka R,Yaguchi T,Gotoh K,Maeda Y,Furuta Y,Kurakawa T,Goto N,Yasunaga T,Narazaki M,Kumanogoh A,Horii T,Iida T,Takeda K,Nakamura S

    更新日期:2017-02-15 00:00:00