A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types.

Abstract:

:A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permutations of all nucleic acid extraction steps were compared. The final modular protocol is suitable for extractions from igneous rock, air, water, and sediments. Sediments range from high-biomass, organic rich coastal samples to samples from the most oligotrophic region of the world's oceans and the deepest borehole ever studied by scientific ocean drilling. Extraction yields of DNA and RNA are higher than with widely used commercial kits, indicating an advantage to optimizing extraction procedures to match specific sample characteristics. The ability to separate soluble extracellular DNA pools without cell lysis from intracellular and particle-complexed DNA pools may enable new insights into the cycling and preservation of DNA in environmental samples in the future. A general protocol is outlined, along with recommendations for optimizing this general protocol for specific sample types and research goals.

journal_name

Front Microbiol

authors

Lever MA,Torti A,Eickenbusch P,Michaud AB,Šantl-Temkiv T,Jørgensen BB

doi

10.3389/fmicb.2015.00476

subject

Has Abstract

pub_date

2015-05-19 00:00:00

pages

476

issn

1664-302X

journal_volume

6

pub_type

杂志文章
  • Functional Characterization of Two Putative DAHP Synthases of AroG1 and AroG2 and Their Links With Type III Secretion System in Ralstonia solanacearum.

    abstract::Type three secretion system (T3SS) is essential for Ralstonia solanacearum to cause disease in host plants and we previously screened AroG1 as a candidate with impact on the T3SS expression. Here, we focused on two putative DAHP synthases of AroG1 and AroG2, which control the first step of the shikimate pathway, a com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00183

    authors: Zhang W,Li J,Shi X,Hikichi Y,Zhang Y,Ohnishi K

    更新日期:2019-02-12 00:00:00

  • Development of Real-Time PCR Assay to Specifically Detect 22 Bifidobacterium Species and Subspecies Using Comparative Genomics.

    abstract::Bifidobacterium species are used as probiotics to provide beneficial effects to humans. These effects are specific to some species or subspecies of Bifidobacterium. However, some Bifidobacterium species or subspecies are not distinguished because similarity of 16S rRNA and housekeeping gene sequences within Bifidobact...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.02087

    authors: Kim HB,Kim E,Yang SM,Lee S,Kim MJ,Kim HY

    更新日期:2020-08-28 00:00:00

  • Ascertaining the relationship between Salmonella Typhimurium and Salmonella 4,[5],12:i:- by MLVA and inferring the sources of human salmonellosis due to the two serovars in Italy.

    abstract::The current picture of human salmonellosis shows Salmonella Typhimurium and S. 4,[5],12:i:- as the most common serovars in Italy. The aims of this study were to investigate the genetic relationship between these serovars, as well as to test the possibility of inferring sources of human salmonellosis due to S. Typhimur...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00301

    authors: Barco L,Barrucci F,Cortini E,Ramon E,Olsen JE,Luzzi I,Lettini AA,Ricci A

    更新日期:2015-04-27 00:00:00

  • AmrZ and FleQ Co-regulate Cellulose Production in Pseudomonas syringae pv. Tomato DC3000.

    abstract::Pseudomonas syringae pv. tomato DC3000 carries the wssABCDEFGHI operon for the synthesis of acetylated cellulose, whose production is stimulated by increasing the intracellular levels of the second messenger c-di-GMP. This enhances air-liquid biofilm formation and generates a wrinkly colony morphotype in solid media. ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00746

    authors: Pérez-Mendoza D,Felipe A,Ferreiro MD,Sanjuán J,Gallegos MT

    更新日期:2019-04-17 00:00:00

  • Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticle characterization and their activity against Bean Yellow Mosaic Virus and human pathogens.

    abstract::Extracellular agents produced by newly isolated bacterial strains were able to catalyze the synthesis of silver nanoparticles (AgNPs). The most effective isolates were identified as Bacillus pumilus, B. persicus, and Bacillus licheniformis using molecular identification. DLS analysis revealed that the AgNPs synthesize...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00453

    authors: Elbeshehy EK,Elazzazy AM,Aggelis G

    更新日期:2015-05-13 00:00:00

  • Fungal Diversity and Mycotoxins in Low Moisture Content Ready-To-Eat Foods in Nigeria.

    abstract::Low moisture content ready-to-eat foods vended in Nigerian markets could be pre-packaged or packaged at point of sale. These foods are widely and frequently consumed across Nigeria as quick foods. Despite their importance in the daily diets of Nigerians, a comprehensive study on the diversity of fungi, fungal metaboli...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00615

    authors: Ezekiel CN,Oyedele OA,Kraak B,Ayeni KI,Sulyok M,Houbraken J,Krska R

    更新日期:2020-04-09 00:00:00

  • Chemoorganotrophic Bacteria From Lake Fryxell, Antarctica, Including Pseudomonas Strain LFY10, a Cold-Adapted, Halotolerant Bacterium Useful in Teaching Labs.

    abstract::Lake Fryxell, situated in the McMurdo Dry Valleys of Antarctica, is an intriguing aquatic ecosystem because of its perennial ice cover, highly stratified water column, and extreme physicochemical conditions, which collectively restrict lake biodiversity to solely microbial forms. To expand our current understanding of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00156

    authors: Baker JM,Vander Schaaf NA,Cunningham AMG,Hang AC,Reeves CL,Huffman ER,Riester CJ,Madigan MT,Sattley WM

    更新日期:2019-02-06 00:00:00

  • Abundant Intergenic TAACTGA Direct Repeats and Putative Alternate RNA Polymerase β' Subunits in Marine Beggiatoaceae Genomes: Possible Regulatory Roles and Origins.

    abstract::The genome sequences of several giant marine sulfur-oxidizing bacteria present evidence of a possible post-transcriptional regulatory network that may have been transmitted to or from two distantly related bacteria lineages. The draft genome of a Cand. "Maribeggiatoa" filament from the Guaymas Basin (Gulf of Californi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01397

    authors: MacGregor BJ

    更新日期:2015-12-16 00:00:00

  • Biosurveillance of avian influenza and Newcastle disease viruses in the Barda region of Azerbaijan using real time RT-PCR and hemagglutination inhibition.

    abstract::The Azerbaijan State Veterinary Control Service (SVCS) has conducted active serological surveillance for avian influenza (AI) in poultry since 2006, when the first outbreak of AI H5N1 occurred in Azerbaijan. Samples are collected from September to May annually and tested using a hemagglutination inhibition (HI) assay ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01128

    authors: Zeynalova S,Guliyev F,Vatani M,Abbasov B

    更新日期:2015-11-06 00:00:00

  • To Construct an Engineered (S)-Equol Resistant E. coli for in Vitro (S)-Equol Production.

    abstract::(S)-equol is one of the major metabolites of daidzein that is produced by human and animal gut bacteria. Most of the physiological functions of soybean isoflavones, such as anti-oxidative activity, anti-cancer activity, and cardiovascular protection have been ascribed to (S)-equol. However, only 30-50% people contain ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01182

    authors: Li H,Mao S,Chen H,Zhu L,Liu W,Wang X,Yin Y

    更新日期:2018-06-04 00:00:00

  • Elucidating the Influence of Chromosomal Architecture on Transcriptional Regulation in Prokaryotes - Observing Strong Local Effects of Nucleoid Structure on Gene Regulation.

    abstract::Both intrinsic and extrinsic mechanisms regulating bacterial expression have been elucidated and described, however, such studies have mainly focused on local effects on the two-dimensional structure of the prokaryote genome while long-range as well as spatial interactions influencing gene expression are still only po...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.02002

    authors: Krogh TJ,Franke A,Møller-Jensen J,Kaleta C

    更新日期:2020-09-01 00:00:00

  • Modulation of Active Gut Microbiota by Lactobacillus rhamnosus GG in a Diet Induced Obesity Murine Model.

    abstract::Gut microbiota play a key role in the development of metabolic disorders. Defining and correlating structural shifts in gut microbial assemblages with conditions related to metabolic syndrome have, however, been proven difficult. Results from 16S genomic DNA and 16S ribosomal RNA analyses of fecal samples may differ w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00710

    authors: Ji Y,Park S,Park H,Hwang E,Shin H,Pot B,Holzapfel WH

    更新日期:2018-04-10 00:00:00

  • Depth Influences Symbiodiniaceae Associations Among Montastraea cavernosa Corals on the Belize Barrier Reef.

    abstract::In Belize, shallow populations (10 and 16 m) of the coral species Montastraea cavernosa from the back reef and reef crest are genetically differentiated from deeper populations on the fore reef and reef wall (25 and 35 m). Like many species of scleractinian corals, M. cavernosa has an obligate symbiosis with dinoflage...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00518

    authors: Eckert RJ,Reaume AM,Sturm AB,Studivan MS,Voss JD

    更新日期:2020-04-09 00:00:00

  • Multi-Locus Phylogeny and Taxonomy of the Fungal Complex Associated With Rusty Root Rot of Panax ginseng in China.

    abstract::Panax ginseng rusty root rot caused by the Ilyonectria species complex is a devastating disease, and it is one of the main factors contributing to the difficulty in continual cropping. Rusty root rot occurs in all ginseng fields, but little is known about the taxonomy of the fungal pathogen complex, especially Ilyonec...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.618942

    authors: Guan YM,Ma YY,Jin Q,Wang QX,Liu N,Fu YP,Zhang YY,Li Y

    更新日期:2020-12-16 00:00:00

  • Characteristics of Quinolone Resistance in Salmonella spp. Isolates from the Food Chain in Brazil.

    abstract::Salmonella spp. is an important zoonotic pathogen related to foodborne diseases. Despite that quinolones/fluoroquinolones are considered a relevant therapeutic strategy against resistant isolates, the increase in antimicrobial resistance is an additional difficulty in controlling bacterial infections caused by Salmone...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.00299

    authors: Pribul BR,Festivo ML,Rodrigues MS,Costa RG,Rodrigues EC,de Souza MM,Rodrigues DD

    更新日期:2017-03-14 00:00:00

  • Soil Microbiome Is More Heterogeneous in Organic Than in Conventional Farming System.

    abstract::Organic farming system and sustainable management of soil pathogens aim at reducing the use of agricultural chemicals in order to improve ecosystem health. Despite the essential role of microbial communities in agro-ecosystems, we still have limited understanding of the complex response of microbial diversity and comp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.02064

    authors: Lupatini M,Korthals GW,de Hollander M,Janssens TK,Kuramae EE

    更新日期:2017-01-04 00:00:00

  • Phage Therapy in Prostatitis: Recent Prospects.

    abstract::Prostatitis has various etiology including bacterial infection and dysregulated immunity; some of its forms remain a serious therapeutic challenge. Inflammation occurs in all forms of this disorder and is proposed to predispose to the development of prostate cancer (PC). There are reports that phage therapy is effecti...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.01434

    authors: Górski A,Jończyk-Matysiak E,Łusiak-Szelachowska M,Międzybrodzki R,Weber-Dąbrowska B,Borysowski J,Letkiewicz S,Bagińska N,Sfanos KS

    更新日期:2018-06-29 00:00:00

  • Prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan.

    abstract::A multidisciplinary, collaborative project was conducted to determine the prevalence and distribution of soil-borne zoonotic pathogens in Lahore district of Pakistan and ascertain its Public Health Significance. Using a grid-based sampling strategy, soil samples (n = 145) were collected from villages (n = 29, 5 sample...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00917

    authors: Shabbir MZ,Jamil T,Ali AA,Ahmad A,Naeem M,Chaudhary MH,Bilal M,Ali MA,Muhammad K,Yaqub T,Bano A,Mirza AI,Shabbir MA,McVey WR,Patel K,Francesconi S,Jayarao BM,Rabbani M

    更新日期:2015-09-10 00:00:00

  • Occurrence and Characterization of Methicillin Resistant Staphylococcus aureus in Processed Raw Foods and Ready-to-Eat Foods in an Urban Setting of a Developing Country.

    abstract::Infections by methicillin-resistant Staphylococcus aureus (MRSA) are gradually increasing in the community. In this study, we investigated a total of 162 food samples including 112 ready-to-eat (RTE) foods and 40 processed raw meat and fish samples collected from retail vendors in Dhaka, Bangladesh and determined the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00503

    authors: Islam MA,Parveen S,Rahman M,Huq M,Nabi A,Khan ZUM,Ahmed N,Wagenaar JA

    更新日期:2019-03-14 00:00:00

  • Fungal Community, Metabolic Diversity, and Glomalin-Related Soil Proteins (GRSP) Content in Soil Contaminated With Crude Oil After Long-Term Natural Bioremediation.

    abstract::Fungi have increased tolerance to environmental stress (also related to the access of pollutants, e.g., trace elements and polycyclic aromatic hydrocarbons PAHs). The aim of the study was to evaluate the mycobiome and functional diversity of fungi in long-term crude-oil contaminated soils as the potential bioremediato...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.572314

    authors: Gałązka A,Grządziel J,Gałązka R,Gawryjołek K,Ukalska-Jaruga A,Smreczak B

    更新日期:2020-09-17 00:00:00

  • Allergies, Helicobacter pylori and the continental enigmas.

    abstract::Helicobacter pylori, a gastric pathogen, is known to be associated with gastric and duodenal ulcers, and is also a strong risk factor for the development of gastric cancer and lymphoma of the mucosal-associated lymphoid tissue. Ordinarily, this should make a strong case for its eradication at par with any other infect...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00578

    authors: Sitaraman R

    更新日期:2015-06-09 00:00:00

  • Perspectives on the Impact of Sampling Design and Intensity on Soil Microbial Diversity Estimates.

    abstract::Soil bacterial communities have long been recognized as important ecosystem components, and have been the focus of many local and regional studies. However, there is a lack of data at large spatial scales, on the biodiversity of soil microorganisms; national or more extensive studies to date have typically consisted o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01820

    authors: Hermans SM,Buckley HL,Lear G

    更新日期:2019-08-07 00:00:00

  • Analysis of Two Complementary Single-Gene Deletion Mutant Libraries of Salmonella Typhimurium in Intraperitoneal Infection of BALB/c Mice.

    abstract::Two pools of individual single gene deletion (SGD) mutants of S. Typhimurium 14028s encompassing deletions of 3,923 annotated non-essential ORFs and sRNAs were screened by intraperitoneal (IP) injection in BALB/c mice followed by recovery from spleen and liver 2 days post infection. The relative abundance of each muta...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.01455

    authors: Silva-Valenzuela CA,Molina-Quiroz RC,Desai P,Valenzuela C,Porwollik S,Zhao M,Hoffman RM,Andrews-Polymenis H,Contreras I,Santiviago CA,McClelland M

    更新日期:2016-01-05 00:00:00

  • Antiviral Mechanism and Biochemical Basis of the Human APOBEC3 Family.

    abstract::The human APOBEC3 (A3) family (A, B, C, DE, F, G, and H) comprises host defense factors that potently inhibit the replication of diverse retroviruses, retrotransposons, and the other viral pathogens. HIV-1 has a counterstrategy that includes expressing the Vif protein to abrogate A3 antiviral function. Without Vif, A3...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00250

    authors: Imahashi M,Nakashima M,Iwatani Y

    更新日期:2012-07-09 00:00:00

  • Bacterial Community Composition in the Sea Surface Microlayer Off the Peruvian Coast.

    abstract::The sea surface microlayer (SML) is located at the air-sea interface, with microorganisms and organic matter in the SML influencing air-sea exchange processes. Yet understanding of the SML bacterial (bacterioneuston) community composition and assembly remains limited. Availability of organic matter, UV radiation and w...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02699

    authors: Zäncker B,Cunliffe M,Engel A

    更新日期:2018-11-15 00:00:00

  • Experimental Evolution as an Underutilized Tool for Studying Beneficial Animal-Microbe Interactions.

    abstract::Microorganisms play a significant role in the evolution and functioning of the eukaryotes with which they interact. Much of our understanding of beneficial host-microbe interactions stems from studying already established associations; we often infer the genotypic and environmental conditions that led to the existing ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2016.01444

    authors: Hoang KL,Morran LT,Gerardo NM

    更新日期:2016-09-13 00:00:00

  • Iterative Chemical Engineering of Vancomycin Leads to Novel Vancomycin Analogs With a High in Vitro Therapeutic Index.

    abstract::Vancomycin is a glycopeptide antibiotic that inhibits transpeptidation during cell wall synthesis by binding to the D-Ala-D-Ala termini of lipid II. For long, it has been used as a last resort antibiotic. However, since the emergence of the first vancomycin-resistant enterococci in 1987, vancomycin resistance has beco...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01175

    authors: Mishra NM,Stolarzewicz I,Cannaerts D,Schuermans J,Lavigne R,Looz Y,Landuyt B,Schoofs L,Schols D,Paeshuyse J,Hickenbotham P,Clokie M,Luyten W,Van der Eycken EV,Briers Y

    更新日期:2018-06-07 00:00:00

  • Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations.

    abstract::Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts during the course of a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00112

    authors: Carreté L,Ksiezopolska E,Gómez-Molero E,Angoulvant A,Bader O,Fairhead C,Gabaldón T

    更新日期:2019-02-12 00:00:00

  • Same, same but different: symbiotic bacterial associations in GBR sponges.

    abstract::Symbioses in marine sponges involve diverse consortia of microorganisms that contribute to the health and ecology of their hosts. The microbial communities of 13 taxonomically diverse Great Barrier Reef (GBR) sponge species were assessed by DGGE and 16S rRNA gene sequencing to determine intra and inter species variati...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00444

    authors: Webster NS,Luter HM,Soo RM,Botté ES,Simister RL,Abdo D,Whalan S

    更新日期:2013-01-18 00:00:00

  • Qualitative and quantitative proteomic analysis of Vitamin C induced changes in Mycobacterium smegmatis.

    abstract::Vitamin C is a critical dietary nutrient in human which has a wide range of regulatory effects on gene expression and physiology of Mycobacterium tuberculosis that leads to a dormant drug-tolerant phenotype. In the presence of iron, vitamin C shows a high bactericidal activity even in the drug resistant phenotype of M...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00451

    authors: Mishra A,Sarkar D

    更新日期:2015-05-18 00:00:00