Abstract:
:The suprachiasmatic nucleus (SCN) and arcuate nucleus (ARC) have reciprocal connections; catabolic metabolic information activates the ARC and inhibits SCN neuronal activity. Little is known about the influence of the SCN on the ARC. Here, we investigated whether the SCN modulated the sensitivity of the ARC to catabolic metabolic conditions. ARC neuronal activity, as determined by c-Fos immunoreactivity, was increased after a hypoglycemic stimulus by 2-deoxyglucose (2DG). The highest ARC neuronal activity after 2DG was found at the end of the light period (zeitgeber 11, ZT11) with a lower activity in the beginning of the light period (zeitgeber 2, ZT2), suggesting the involvement of the SCN. The higher activation of ARC neurons after 2DG at ZT11 was associated with higher 2DG induced blood glucose levels as compared with ZT2. Unilateral SCN-lesioned animals, gave a mainly ipsilateral activation of ARC neurons at the lesioned side, suggesting an inhibitory role of the SCN on ARC neurons. The 2DG-induced counterregulatory glucose response correlated with increased ARC neuronal activity and was significantly higher in unilateral SCN-lesioned animals. Finally, the ARC as site where 2DG may, at least partly, induce a counterregulatory response was confirmed by local microdialysis of 2DG. 2DG administration in the ARC produced a higher increase in circulating glucose compared with 2DG administration in surrounding areas such as the ventromedial nucleus of the hypothalamus (VMH). We conclude that the SCN uses neuronal pathways to the ARC to gate sensory metabolic information to the brain, regulating ARC glucose sensitivity and counterregulatory responses to hypoglycemic conditions.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Herrera-Moro Chao D,León-Mercado L,Foppen E,Guzmán-Ruiz M,Basualdo MC,Escobar C,Buijs RMdoi
10.1210/en.2015-1751subject
Has Abstractpub_date
2016-09-01 00:00:00pages
3439-51issue
9eissn
0013-7227issn
1945-7170journal_volume
157pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::MCF-7 cells migrate through vitronectin-coated filters in response to insulin-like growth factor I (IGF-I); migration is inhibited by the matrix metalloproteinase (MMP) inhibitor BB-94, but not by the serine proteinase inhibitor aprotinin. MMP-9 was identified in the conditioned medium of MCF-7 cells; in addition, flu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.4.6623
更新日期:1999-04-01 00:00:00
abstract::Chronic exposure to stressors impairs the function of multiple organ systems and has been implicated in increased disease risk. In the rodent, the chronic variable stress (CVS) paradigm has successfully modeled several stress-related illnesses. Despite striking disparities between men and women in the prevalence and e...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00217
更新日期:2018-07-01 00:00:00
abstract::Amplification of insulin secretion by cAMP is mediated by protein kinase A (PKA) and exchange protein directly activated by cAMP (Epac). Using selective activators, we determined how each effector influences the cytosolic free Ca(2+) concentration ([Ca(2+)]c) and insulin secretion in mouse islets. Alone PKA activator ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1247
更新日期:2014-09-01 00:00:00
abstract::Meiotically arrested mammalian oocytes are stimulated to resume meiosis by LH. This response, which can be reversed by elevation of intraoocyte cAMP levels, is associated with interruption of gap junctional communication (GJC) within the ovarian follicle. In the present study, we examined the hypothesis that disruptio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1011
更新日期:2006-05-01 00:00:00
abstract::Corticotropin-releasing factor receptor type 2beta, expressed in the rodent cardiovascular system, is a member of the G protein-coupled receptor family. This receptor is coupled positively to adenylate cyclase and is bound preferentially by the urocortin (Ucn)-related peptides (Uncs): Ucn, Ucn II, and Ucn III. In the ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0023
更新日期:2003-06-01 00:00:00
abstract::We have recently demonstrated that fibroblast growth factor (FGF)-2 promotes neuroblastoma cell differentiation and overrides their mitogenic response to IGF-I. However, the mechanisms involved are unknown. SK-N-MC cells were cultured with FGF-2 (50 ng/ml) and/or IGF-I (100 ng/ml) up to 48 h. Fluorescence-activated ce...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1797
更新日期:2009-09-01 00:00:00
abstract::Newly discovered kisspeptin (metastin), encoded by the Kiss1/KISS1 gene, is considered as a major gatekeeper of puberty through the regulation of GnRH. In the present study, we cloned a novel kisspeptin gene (kiss2) in the zebrafish Danio rerio and the medaka Oryzias latipes, which encodes a sequence of 125 and 115 am...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0940
更新日期:2009-02-01 00:00:00
abstract::The G protein α-subunit G(s)α mediates receptor-stimulated cAMP generation. Heterozygous inactivating G(s)α mutations on the maternal allele result in obesity primarily due to reduced energy expenditure in Albright hereditary osteodystrophy patients and in mice. We previously showed that mice with central nervous syst...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1113
更新日期:2012-09-01 00:00:00
abstract::VEGF is produced by osteoblasts and has been postulated to function as an angiogenic stimulus during normal skeletal development and in fracture repair. In this study, we characterized the molecular mechanisms by which experimental hypoxia increases VEGF mRNA in human MG63 osteoblast-like cells. Exposure of MG63 cells...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.8112
更新日期:2001-02-01 00:00:00
abstract::In vivo, fibroblast growth factor-2 (FGF-2) inhibits longitudinal bone growth. Similarly, activating FGF receptor 3 mutations impair growth in achondroplasia and thanatophoric dysplasia. To investigate the underlying mechanisms, we chose a fetal rat metatarsal organ culture system that would maintain growth plate hist...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.6.6032
更新日期:1998-06-01 00:00:00
abstract::Tuberoinfundibular peptide is a recently discovered agonist for the PTH receptor-2; the latter has a wide distribution including the external zone of the median eminence of the hypothalamus, suggesting a role in neuroendocrine function. We have investigated the effects of tuberoinfundibular peptide on the hypothalamo-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8308
更新日期:2001-08-01 00:00:00
abstract::Two receptors (CRH receptor type 1 and CRH receptor type 2) have been identified for the stress-induced neuropeptide, CRH and related peptides, urocortin, and urocortin II. We previously found marked down-regulation of cardiac CRH receptor type 2 expression following administration of bacterial endotoxin, lipopolysacc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8342
更新日期:2001-08-01 00:00:00
abstract::Enhanced epinephrine secretion from adrenal chromaffin cells (ACCs) is an important homeostatic response to severe systemic inflammation during sepsis. Evidence suggests that increased activation of ACCs by preganglionic sympathetic neurons and direct alterations in ACC function contribute to this response. However, t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1623
更新日期:2014-01-01 00:00:00
abstract::In this study, we demonstrate that disruption of neuroendocrine signaling is a major factor driving disease progression in myelin oligodendrocyte glycoprotein-induced chronic relapsing experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. Although the initial episode of chronic relapsing ex...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.8.8292
更新日期:2001-08-01 00:00:00
abstract::To investigate trafficking in neuroendocrine cells, green fluorescent protein (GFP) tags were fused to various portions of the preproneuropeptide Y (NPY) precursor. Two neuroendocrine cell lines, AtT-20 corticotrope tumor cells and PC-12 pheochromocytoma cells, along with primary anterior pituitary cells, were examine...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.2.7929
更新日期:2001-02-01 00:00:00
abstract::Previous experiments showed that LSH/SsLak female hamsters cease ovarian cyclicity after 2-4 weeks of short photoperiod (SP) treatment. This study was designed to reveal early hormonal and histological changes on day 1 of the estrous cycle (estrus) in SP-treated animals and changes brought about by the SP-induced acyc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-1-239
更新日期:1987-01-01 00:00:00
abstract::Homogenates from male ddy mouse sublingual glands were incubated with 100 nM [125I]rT3 in the presence of 2 mM dithiothreitol (DTT). Metabolites were analyzed by HPLC or Dowex-50 minicolumn assay. 3,3'-Diiodothyronine and I- were the only appreciable products. Iodide release from rT3 was compatible with the enzymatic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.3.8440180
更新日期:1993-03-01 00:00:00
abstract::Defining the molecular genetics of diabetes gives new insight into the underlying etiology and so should help improve treatment. The genetic etiology is now known for most patients with beta-cell monogenic diabetes, allowing genetic classification. We review how this genetic knowledge alters treatment. Patients with a...
journal_title:Endocrinology
pub_type: 杂志文章,评审
doi:10.1210/en.2006-0152
更新日期:2006-06-01 00:00:00
abstract::Properties of cyclic 3',5'-nucleotide phosphodiesterase in the 100,500 X g supernatant of the bovine thyroid were investigated. The enzyme activity was measured by a radioisotopic method using an anionic-exchange resin, and it was found that the activity was stimulated by Mg2+. Sephadex G-200 gel filtration separated ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-5-1534
更新日期:1978-11-01 00:00:00
abstract::We have previously reported that neurotensin (NT) is released from the small intestine and elevated in the hepatic-portal circulation in response to the perfusion of the small intestine with a micellar solution of oleic acid. In order to determine the minimum acyl chain length and whether the presence of a carboxylic ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1133
更新日期:1985-03-01 00:00:00
abstract::In bone, early events in inflammation involve the production and release of primary proinflammatory cytokines, such as interleukin-1 beta. By activation of target cells, these cytokines are thought to induce a second wave of cytokines, including monocyte chemoattractant protein-1 (MCP-1). MCP-1 is a cytokine that stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.6.7750500
更新日期:1995-06-01 00:00:00
abstract::Insulin-like growth factors (IGFs), when isolated from serum or tissue fluids, are usually found as part of a protein complex which also contains one of several IGF binding proteins (IGFBPs). Although some IGFBPs have been shown to alter interactions of IGFs with their receptors in vitro and can modify the responses o...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.2.7507840
更新日期:1994-02-01 00:00:00
abstract::Commercially available preparations of natural porcine glucose-dependent insulinotropic polypeptide (GIP) were subjected to reverse phase HPLC. The material was found to give rise to 4 peaks which were characterized by HPLC-retention time and N-terminal sequence analysis. They represented: intact porcine GIP(1-42), 58...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-835
更新日期:1987-02-01 00:00:00
abstract::GH and IGF-I control over 80% of postnatal growth. We recently established that TNFalpha impairs the ability of IGF-I to increase protein synthesis and promote expression of myogenin in myoblasts. Here we extend these results by showing that ceramide, a second messenger in both TNFalpha and IL-1beta receptor signaling...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1749
更新日期:2004-10-01 00:00:00
abstract::A rat medullary thyroid carcinoma, which was previously shown to produce high levels of immunoreactive cholecystokinin (CCK), was used to establish a stable cell line. Transplantable tumors were subjected to four series of alternate in vitro and in vivo passages. Cells were prepared from the fourth series of tumors un...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-850
更新日期:1989-08-01 00:00:00
abstract::In previous studies we demonstrated a much greater rate of glucose cycling (glucose-->glucose-6-P-->glucose) in islets from ob/ob mice than from lean litter mates. Cycling was further augmented by dexamethasone treatment. To determine whether these findings could be accounted for by increased islet glucose-6-phosphata...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.136.5.7720640
更新日期:1995-05-01 00:00:00
abstract::The inhibitor of apoptosis proteins (IAPs) constitutes a family of highly conserved apoptosis suppressor proteins that were originally identified in baculoviruses. Although IAP homologs have recently been demonstrated to suppress apoptosis in mammalian cells, their expression and role in human ovarian epithelial cance...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.1.7897
更新日期:2001-01-01 00:00:00
abstract::Early life exposure to immunological challenge has programming effects on the adult hypothalamo-pituitary-adrenocortical axis stress responsivity, and stress is known to suppress GnRH pulse generator activity, especially LH pulses. We investigated the effects of neonatal exposure to endotoxin on stress-induced suppres...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-0710
更新日期:2007-12-01 00:00:00
abstract::Central administration of melanocortin 3 and 4 receptor (MC3/4-R) agonists increases energy expenditure, with the hypothalamus commonly held as the primary site of action. It is also clear, however, that MC4-R are expressed in caudal brainstem structures of relevance to the control of energy expenditure. Three experim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0440
更新日期:2003-11-01 00:00:00
abstract::An assay was developed to detect tritium-labeled ovine FSH beta-subunit [( 3H]oFSH beta) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSH beta (RCM-oFSH beta) in a two-cycle immunoextraction procedure. A discrete species...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-1-154
更新日期:1984-07-01 00:00:00