Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management.

Abstract:

BACKGROUND:CD34 flow cytometry is the gold standard for stem cell enumeration in peripheral blood at the mobilization stage and in the final apheresis product. The new stem cell mode of the Sysmex XN Series analyzer enumerates an immature cell population in the white progenitor and pathological cell (WPC) channel, based on the cell size, internal cellular complexity, and fluorescence intensity. STUDY DESIGN AND METHODS:In this multicenter study we analyzed 147 peripheral blood samples, 22 samples during collection of stem cells, and 45 samples from the apheresis product of 18 healthy allogeneic donors and 84 autologous patients. RESULTS:In this multicenter study we demonstrate that the XN stem cell enumeration method correlates well with viable CD34+ cells determined by flow cytometry during the stem cell mobilization phase to determine apheresis start time, during apheresis for real-time monitoring and adjustment, and for quality control of the final stem cell harvest. CONCLUSION:Our data show that there is an improvement in the correlation of XN stem cells and CD34+ cells in the peripheral blood during stem cell mobilization as well as in stem cell harvests compared to SE or XE Series analyzers. The XN stem cell enumeration method has a number of advantages compared to CD34 flow cytometry: it is fast, simple, reproducible, and less expensive. CE marking for the European market has been obtained, making the stem cell count on the XN analyzer a reportable clinical variable.

journal_name

Transfusion

journal_title

Transfusion

authors

Grommé M,Russcher H,Braakman E,Klinkspoor JH,Dobber JA,de Greef I,de Wit NCJ

doi

10.1111/trf.14183

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

1949-1955

issue

8

eissn

0041-1132

issn

1537-2995

journal_volume

57

pub_type

杂志文章,多中心研究
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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.35795357880.x

    authors: Hauser R

    更新日期:1995-07-01 00:00:00

  • UMC, another Cromer-related blood group antigen.

    abstract::An antibody found in the serum of a Japanese blood donor detects a new high-frequency red cell antigen named UMC. UMC is absent from Inab phenotype cells, weakly expressed on Dr(a-) cells, and destroyed by alpha-chymotrypsin but not trypsin treatment of red cells. UMC is, therefore, a Cromer-related antigen. Immunoblo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1989.29990070184.x

    authors: Daniels GL,Okubo Y,Yamaguchi H,Seno T,Ikuta M

    更新日期:1989-11-01 00:00:00

  • High prevalence of infectious events in thrombotic thrombocytopenic purpura and genetic relationship with toll-like receptor 9 polymorphisms: experience of the French Thrombotic Microangiopathies Reference Center.

    abstract:BACKGROUND:Infectious events have been reported as major environmental triggers of thrombotic thrombocytopenic purpura (TTP). We detail here the potential association between infections and TTP. STUDY DESIGN AND METHODS:We recruited randomly and prospectively a cohort of 280 consecutive TTP patients during a 9-year pe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12263

    authors: Morgand M,Buffet M,Busson M,Loiseau P,Malot S,Amokrane K,Fortier C,London J,Bonmarchand G,Wynckel A,Provôt F,Poullin P,Vanhille P,Presne C,Bordessoule D,Girault S,Delmas Y,Hamidou M,Mousson C,Vigneau C,Lautrette A

    更新日期:2014-02-01 00:00:00

  • Collection of mobilized blood progenitor cells for hematopoietic rescue by large-volume leukapheresis.

    abstract:BACKGROUND:Mobilized blood progenitor cells rapidly reconstitute hematopoiesis in patients after dose-intensive chemotherapy. However, optimal timing and methods of mobilized blood progenitor cell collection have yet to be fully defined. STUDY DESIGN AND METHODS:The utility of large-volume leukapheresis (LVL; > 15 L b...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.35695288768.x

    authors: Comenzo RL,Vosburgh E,Weintraub LR,Tansan S,Arkin CF,Wright DG

    更新日期:1995-06-01 00:00:00

  • NBTS/BRIC 8. A monoclonal anti-C3d antibody.

    abstract::A stable mouse hybridoma line secreting monoclonal anti-C3d antibody (NBTS/BRIC 8) was produced. Ascitic fluid containing BRIC 8 can be used at a final dilution of 1 to 5000 to provide an excellent antiglobulin reagent when mixed with a conventional polyclonal rabbit anti-human IgG serum. ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25385219912.x

    authors: Holt PD,Donaldson C,Judson PA,Johnson P,Parsons SF,Anstee DJ

    更新日期:1985-05-01 00:00:00

  • Flow cytometric determination of RBC survival in autoimmune hemolytic anemia.

    abstract:BACKGROUND:In cases of warm autoimmune hemolytic anemia (WAIHA), crossmatch incompatible RBCs are most often used for transfusion. The determination of the in vivo survival of transfused and autologous RBCs in WAIHA is helpful in the assessment of the efficacy of transfusion and other therapeutic interventions. CASE R...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41040493.x

    authors: Zeiler T,Müller JT,Hasse C,Kullmer J,Kretschmer V

    更新日期:2001-04-01 00:00:00

  • Evaluation of the National Healthcare Safety Network Hemovigilance Module for transfusion-related adverse reactions in the United States.

    abstract:INTRODUCTION:The National Healthcare Safety Network (NHSN) Hemovigilance Module (HM) collects data on the frequency, severity, and imputability of transfusion-associated adverse events. These events contribute to significant morbidity and mortality among transfusion patients. We report results from the first systematic...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15008

    authors: Edens C,Haass KA,Cumming M,Osinski A,O'Hearn L,Passanisi K,Eaton L,Visintainer P,Savinkina A,Kuehnert MJ,Basavaraju SV,Andrzejewski C

    更新日期:2019-02-01 00:00:00

  • Selective protection of RBCs against photodynamic damage by the band 3 ligand dipyridamole.

    abstract:BACKGROUND:All studied photosensitizers for virus inactivation impair RBCs. To reduce damage to the RBCs without affecting virucidal activity, selective protection of the RBCs is necessary. The ability of the band 3 ligand, dipyridamole, to react with singlet oxygen and to increase the selectivity of photosterilization...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40111330.x

    authors: vanSteveninck J,Trannoy LL,Besselink GA,Dubbelman TM,Brand A,de Korte D,Verhoeven AJ,Lagerberg JW

    更新日期:2000-11-01 00:00:00

  • A comparison of results obtained by two different chromium-51 methods of determining platelet survival and recovery.

    abstract::A number of different methods exist that use chromium-51 to determine platelet survival and recovery. A 1986 article in TRANSFUSION described the recommended methods for conducting such studies in the hope of standardizing methodology and permitting interlaboratory comparison of results. The results obtained with the ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30590296371.x

    authors: Rock G,Tittley P

    更新日期:1990-06-01 00:00:00

  • Hemostatic profile under fluid resuscitation during rivaroxaban anticoagulation: an in vitro survey.

    abstract:BACKGROUND:Uncontrollable bleeding is the leading cause of death in traumatically injured patients. The extent to which direct factor Xa inhibitors interfere with the applied resuscitation measures is presently unknown. STUDY DESIGN AND METHODS:In this study, we investigated the effect of the resuscitation fluids sali...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14933

    authors: Helin TA,Zuurveld M,Manninen M,Meijers JCM,Lassila R,Brinkman HJM

    更新日期:2018-12-01 00:00:00

  • Platelet crossmatches of single-donor platelet concentrates using a latex agglutination assay.

    abstract::A latex agglutination assay was evaluated for the purpose of identifying compatible platelet donors for alloimmunized recipients. Assay reagents were prepared by adsorbing detergent-solubilized, donor-specific platelets to polystyrene latex beads. Semiquantitative results for up to 30 donors can be completed in less t...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33893342745.x

    authors: Ogden DM,Asfour A,Koller C,Lichtiger B

    更新日期:1993-08-01 00:00:00

  • Platelets photochemically treated with amotosalen HCl and ultraviolet A light correct prolonged bleeding times in patients with thrombocytopenia.

    abstract:BACKGROUND:Photochemical treatment (PCT) with amotosalen HCl with ultraviolet A illumination inactivates pathogens and white blood cells in platelet (PLT) concentrates. STUDY DESIGN AND METHODS:In a Phase II crossover study, 32 patients with thrombocytopenia received one transfusion of PCT and/or one transfusion of un...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2006.00791.x

    authors: Slichter SJ,Raife TJ,Davis K,Rheinschmidt M,Buchholz DH,Corash L,Conlan MG

    更新日期:2006-05-01 00:00:00

  • Effects of a multimodality blood conservation schema toward improvement of intraoperative hemoglobin levels and off-pump transfusions in coronary artery bypass graft surgery.

    abstract:BACKGROUND:Cardiothoracic surgery places significant demands on blood bank resources. Measures aimed at reducing intraoperative hemodilution were initiated as part of a blood conservation program. STUDY DESIGN AND METHODS:We initiated a series of measures aimed at reducing hemodilution volume: 1) reduction of intraven...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12745

    authors: Tran MH,Lin DM,Wilcox T,Schiro D,Cannesson M,Milliken J

    更新日期:2014-10-01 00:00:00

  • Passenger lymphocyte syndrome with severe hemolytic anemia due to an anti-Jk(a) after allogeneic PBPC transplantation.

    abstract:BACKGROUND:After allogeneic peripheral blood progenitor cell (PBPC) transplantation, a patient developed a severe hemolytic transfusion reaction due to passenger lymphocyte syndrome. CASE REPORT:A 50-year-old woman with secondary acute myeloid leukemia transforming from a myelodysplastic syndrome received an ABO-compa...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40060632.x

    authors: Leo A,Mytilineos J,Voso MT,Weber-Nordt R,Liebisch P,Lensing C,Schraven B

    更新日期:2000-06-01 00:00:00

  • Monitoring for underutilization of RBC components and platelets.

    abstract:BACKGROUND:Monitoring blood transfusion for overutilization is standard practice at most institutions. STUDY DESIGN AND METHODS:This study monitored for underutilization of blood transfusion over a 14-month period, by evaluating patients who had Hb levels that were reported to be <5 g per dL or platelet counts <10 x 1...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41050587.x

    authors: Saxena S,Wehrli G,Makarewicz K,Sartorelli J,Shulman IA

    更新日期:2001-05-01 00:00:00

  • Depressed blood group antigens on red cells from a Mexican donor.

    abstract::Red cells from a Mexican blood donor had depressed H, IF, ID, i, Sda, Sdx, D, LW, Dib, Vel and JMH antigens. A wide range of other blood group antigens had normal activity. The unusual red cell phenotype may be caused by a membrane anomaly that alters orientation of certain cell surface components. The specificities o...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1983.23183147309.x

    authors: Biro V,Garratty G,Johnson CL,Marsh WL

    更新日期:1983-01-01 00:00:00

  • Different growth kinetics in blood components and genetic analysis of Lactococcus garvieae isolated from platelet concentrates.

    abstract:BACKGROUND:In 2014, we experienced the first isolation of Lactococcus garvieae from a platelet concentrate (PC). Thereafter, L. garvieae contamination of PCs occurred in two more cases in Japan. It is rare that bacterial contamination with uncommon strains like this species occurs frequently within a short period. Ther...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15836

    authors: Kozakai M,Matsumoto C,Matsumoto M,Takakura A,Matsubayashi K,Satake M

    更新日期:2020-07-01 00:00:00

  • Red blood cell alloimmunization in transfused patients with bone marrow failure syndromes.

    abstract:BACKGROUND:Red blood cell (RBC) alloimmunization is a concern for patients who receive multiple or chronic transfusions. Alloimmunization prevalence in transfused patients with bone marrow failure syndrome (BMFS) is unknown. This study aimed to determine physician practice for RBC antigen matching, immunization rates, ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13608

    authors: Cohen D,Hartung H,Evans P,Friedman DF,Chou ST

    更新日期:2016-06-01 00:00:00

  • Effect of storage of red blood cells on alloimmunization.

    abstract:BACKGROUND:Red blood cells (RBCs) undergo changes during storage. Various studies have suggested a higher risk of adverse and often multifactorial clinical outcomes associated with older-stored RBCs. Our aim therefore was to examine if storage of transfused RBCs is also associated with the risk of RBC-specific alloanti...

    journal_title:Transfusion

    pub_type: 杂志文章,多中心研究

    doi:10.1111/trf.12156

    authors: Zalpuri S,Schonewille H,Middelburg R,van de Watering L,de Vooght K,Zimring J,van der Bom JG,Zwaginga JJ

    更新日期:2013-11-01 00:00:00

  • Effects of new brochures on blood donor recruitment and retention.

    abstract:BACKGROUND:Currently, donors may arrive at blood collection sites without prior knowledge of eligibility and deferral criteria. STUDY DESIGN AND METHODS:The effects of distributing newly developed recruitment brochures 2 weeks in advance of blood drives and the provision of brochures on temporary deferral at the takin...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1537-2995.1994.34794330012.x

    authors: Gimble JG,Kline L,Makris N,Muenz LR,Friedman LI

    更新日期:1994-07-01 00:00:00

  • The use of polymerase chain reaction in plasma pools for the concomitant detection of hepatitis C virus and HIV type 1 RNA.

    abstract:BACKGROUND:Detection of viral nucleic acids might increase blood transfusion safety through the detection of recently infected blood donors during the preseroconversion window period. Individual screening is difficult to apply, because of technical and financial constraints. STUDY DESIGN AND METHODS:A polymerase chain...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.381098440854.x

    authors: Yerly S,Pedrocchi M,Perrin L

    更新日期:1998-10-01 00:00:00

  • How we handled the dextran shortage: an alternative washing or dilution solution for cord blood infusions.

    abstract::Dextran 40 is the main component of the solution used to wash or dilute thawed cord blood unit (CBU) products for stem cell transplant. Dextran 40 became unavailable in the United States as of April 2014. Like many other cellular therapy laboratories in the United States, we found ourselves with limited dextran 40 inv...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13015

    authors: Reich-Slotky R,Bachegowda LS,Ancharski M,Mendeleyeva L,Rubinstein P,Rennert H,Shore T,van Besien K,Cushing M

    更新日期:2015-06-01 00:00:00

  • Quality of red blood cells washed using the ACP 215 cell processor: assessment of optimal pre- and postwash storage times and conditions.

    abstract:BACKGROUND:Washing of red blood cell concentrates (RCCs) is required for potassium-sensitive transfusion recipients, including neonates in need of large-volume transfusions. When open, nonsterile washing systems are used, postwash outdate time is limited to 24 hours, often leading to problems providing the component to...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12170

    authors: Hansen A,Yi QL,Acker JP

    更新日期:2013-08-01 00:00:00

  • Dynamics of shape recovery by stored red blood cells during washing at the single cell level.

    abstract:BACKGROUND:Hypothermic storage transforms red blood cells (RBC) from smooth biconcave discocytes into increasingly spherical spiculated echinocytes and, ultimately, fragile spherocytes (S). Individual cells undergo this transformation at different rates, producing a heterogeneous mixture of RBCs at all stages of echino...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15979

    authors: Lu M,Shevkoplyas SS

    更新日期:2020-08-04 00:00:00

  • Retrograde transmission of Proteus mirabilis during platelet transfusion and the use of arbitrarily primed polymerase chain reaction for bacteria typing in suspected cases of transfusion transmission of infection.

    abstract:BACKGROUND:When bacteria are found, after a platelet transfusion, in the recipient's blood as well as in the platelet concentrate (PC), a causal relationship is normally suspected, with the PC as the causative agent. The other alternative, that the patient has bacteremia and contaminated the PC, is less well documented...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1995.351096026371.x

    authors: Engstrand M,Engstrand L,Högman CF,Hambraeus A,Branth S

    更新日期:1995-10-01 00:00:00

  • Exchange transfusion in sickle cell disease using a continuous-flow blood cell separator.

    abstract::An exchange transfusion was performed preoperatively on a patient with sickle cell disease using a continuous-flow blood cell separator. An exchange of 2,825 ml red blood cells achieved a hemoglobin A level of 90.8 per cent. The continuous-flow blood cell separator appears to offer a safe and effective method of excha...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17377196364.x

    authors: Kernoff LM,Botha MC,Jacobs P

    更新日期:1977-05-01 00:00:00

  • Hemolytic transfusion reaction due to anti-Tc(a).

    abstract:BACKGROUND:Anti-Tc(a) detects a high-incidence antigen in the Cromer blood group system. Cromer system antibodies have not usually been associated with hemolytic transfusion reactions or hemolytic disease of the newborn. CASE REPORT:Anti-Tc(a) (initially identified in the patient's serum in 1982) was not detected when...

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    doi:10.1046/j.1537-2995.1999.39090948.x

    authors: Kowalski MA,Pierce SR,Edwards RL,Ethirajan S,Ryan K,Livergood MP

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  • Evaluation of an experimental filter designed for improving the quality of red blood cells (RBCs) during storage by simultaneously removing white blood cells and immunomodulators and improving RBC viscoelasticity and Band 3 proteins.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12330

    authors: Sowemimo-Coker SO

    更新日期:2014-03-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14957

    authors: Gravemann U,Handke W,Lambrecht B,Schmidt JP,Müller TH,Seltsam A

    更新日期:2018-11-01 00:00:00

  • A rapid method for harvesting autologous red cells from patients with hemoglobin S disease.

    abstract::Red cells from patients with sickle cell disease (HbSS and HbSC) are more resistant to lysis in hypotonic NaCl solutions than normal (HbAA) red cells. Taking advantage of this inherent resistance to osmotic stress, patient red cells (HbSS or HbSC) were rapidly isolated from donor red cells (HbAA or HbAS) by washing wi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28188127945.x

    authors: Brown DJ

    更新日期:1988-01-01 00:00:00