Molecular epidemiology of Bartonella species isolated from ground squirrels and other rodents in northern California.

Abstract:

:Bartonella spp. are endemic in wild rodents in many parts of the world. A study conducted in two northern California counties (Sonoma and Yolo) sampling California ground squirrels (Otospermophilus beecheyi) and four other rodent species (Peromyscus maniculatus, P. boylii, P. truei and Neotoma fuscipes) led to the isolation of small Gram-negative bacilli which were identified as Bartonella spp. based on colony morphology, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and partial gene sequencing. Overall, Bartonella spp. were isolated from the blood of 71% (32/45) of the ground squirrels and one third (22/66) of the other rodents. PCR-RFLP analysis of the gltA and 16S rRNA genes yielded seven unique profiles, four for the ground squirrels and three for the other rodents. Isolates from each PCR-RFLP profiles were submitted for partial sequencing. Ground squirrel isolates were most closely related to B. washoensis, whereas the other rodent isolates were closest to B. vinsonii subsp. vinsonii and B. vinsonii subsp. arupensis. Two of these three species or subspecies are known zoonotic agents.

journal_name

Epidemiol Infect

authors

Ziedins AC,Chomel BB,Kasten RW,Kjemtrup AM,Chang CC

doi

10.1017/S0950268816000108

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

1837-44

issue

9

eissn

0950-2688

issn

1469-4409

pii

S0950268816000108

journal_volume

144

pub_type

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