Abstract:
:Alcohol dehydrogenases (ADH) are key enzymes of ethanol metabolism that mediate its oxidation to acetaldehyde. ADHs are also able to oxidize some types of neurotransmitters such as dopamine, serotonin and norepinephrine. Increased level of ADHs activity, induced by chronic alcohol consumption, is presumably associated with disturbed neurotransmitters metabolism that leads to stable alcohol craving. As earlier reported, intraperitoneal administration of 4-methilpirasole (non-specific inhibitor of ADHs) has shown to provide a short-term anti-alcoholic effect, but individual roles of ADH isoforms in this process were still unclear. The aim of this work was to study the roles of brain and serum ADH isoforms in alcohol consumption and neurotransmitter metabolism in the rats. In the study we used specific-pathogen-free (SPF) Wistar rats chronically alcoholized with 15% ethanol. 4-methilpirasole intranasal administration in small doses led to local inhibition of ADH III activity in the brain estimated by spectrophotometric assay. It correlated with dose-dependent reduction of dopamine concentration and increased level of its metabolic products in the brain but did not influence alcohol consumption. These data allowed us to propose an important role of brain ADHs (predominantly ADH III) in metabolism of dopamine in chronically alcoholized rats but not in regulation of alcohol consumption. To evaluate the role of serum ADH isoforms we immunized the rats with recombinant horse ADH that led to production of high levels of cross-reactive anti-ADH antibodies verified by ELISA assay. Immunization led to 30% decrease in alcohol consumption and recovery of general behavioral parameters such as motor activity, anxiety and depression level. At the same time active immunization did not cause any impairments in animal blood composition. We can conclude that immunization against ADHs appeared to be a safe way to decrease alcohol consumption that could be possibly associated with neurotransmitters metabolism correction.
journal_name
Physiol Behavjournal_title
Physiology & behaviorauthors
Pavshintsev VV,Mitkin NA,Frolova OY,Kushnir EA,Averina OA,Lovat MLdoi
10.1016/j.physbeh.2017.07.022subject
Has Abstractpub_date
2017-10-01 00:00:00pages
458-466eissn
0031-9384issn
1873-507Xpii
S0031-9384(17)30221-4journal_volume
179pub_type
杂志文章abstract::Rats were concurrently exposed to a sustained avoidance/escape (SAE) procedure and a procedure for assessing demand for food. In the sustained SAE procedure, signalled shock avoidance/escape trials were presented at varying intervals averaging five minutes. The shock could be avoided by pulling on a ceiling chain earl...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(91)90288-y
更新日期:1991-03-01 00:00:00
abstract::Applying high temperature cooling concepts, i.e. high temperature cooling (T(supply) is 16-20°C) HVAC systems, in the built environment allows the reduction in the use of (high quality) energy. However, application of high temperature cooling systems can result in whole body and local discomfort of the occupants. Non-...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2012.07.008
更新日期:2012-09-10 00:00:00
abstract::In these experiments we determined whether stress influenced intake of different flavored test solutions or only those that were preferred. In a series of studies, rats or hamsters were exposed to acute (1 h) or repeated (3 h/day for 3 days) restraint stress immediately followed by access to one of four tastants (sacc...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(98)00223-6
更新日期:1999-01-01 00:00:00
abstract::A cataleptic animal clings in a vertical position, unmoving, for abnormally long periods by supporting some of its weight on its hindlegs, grasping with the forepaws, flexing its forelimbs, and holding the head horizontal. When the head is snugly wrapped with a bandage, the head slowly falls backward, the neck hyperex...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(86)90188-5
更新日期:1986-01-01 00:00:00
abstract::Effects of various doses (0-250 micrograms/kg, SC) of estradiol-17 beta (E2) in a two-bottle choice conditioned taste aversion and a two-compartment conditioned place preference procedure were studied in male and female rats. Dose-dependent taste aversion and place aversion effects of E2 were established, and the cond...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(91)90427-p
更新日期:1991-11-01 00:00:00
abstract::Mixtures of compounds can often be tasted even when all of their components are too weak to be tasted separately. Such mixtures are said to be integrative. Integration was demonstrated by mixing compounds in concentrations proportional to their separate detection thresholds and then measuring the detection threshold o...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(97)00270-9
更新日期:1997-11-01 00:00:00
abstract::Three experiments examined the hypothesis that social isolation of weanling rats potentiates hedonic processes by examining the consumption of sucrose solutions. In the first experiment no differences in consumption were found between socially reared rats and isolation-reared rats allowed to consume sucrose in a famil...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(97)00116-9
更新日期:1997-08-01 00:00:00
abstract::Ovarian hormones have been shown to regulate liver lipid accumulation in rats. The present study was designed to evaluate liver lipid resorption in ovariectomized (Ovx) rats. Ovx and sham-operated (Sham) rats were submitted to a high-fat (HF; 43% kcal fat as energy) diet for 5 weeks and then either maintained on this ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2008.05.024
更新日期:2008-09-03 00:00:00
abstract::Exercise-related affective responses, particularly in-task, have been implicated as influencing behavioral maintenance. An extensive body of literature, primarily using aerobic modes in laboratory settings, has identified a relatively consistent inverse relationship between intensity and affect. This relationship is p...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2020.113261
更新日期:2021-03-01 00:00:00
abstract::The effect of cage size on spontaneous locomotor rhythms of laboratory mice was studied under simulated light-dark (12:12) cycles. On-line image analysis of bodily displacement yielded a locomotor signal over a period of 3 days. Continuous wavelet transform was applied to the signal, and ensemble averaging of eight mi...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(97)00305-3
更新日期:1997-12-01 00:00:00
abstract::Temporal modification of amygdaloid serotonin (5HT) content and the resultant muricide behavior, compared to isolated and olfactory bulbectomized rats, were studied after chronic theophylline administration. Theophylline raised amygdaloid 5HT after Day 28 and amygdaloid 5-hydroxyindoleacetic acid (5HIAA), its deaminat...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(75)90213-9
更新日期:1975-10-01 00:00:00
abstract::Rabbits (Oryctolagus cuniculus), like many small mammals, have a marked postpartum estrus, and during the breeding season, are often both pregnant and lactating. We examined reproductive performance and allocation of resources by rabbit mothers with overlapping litters under presumably optimal conditions of unrestrict...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2004.05.011
更新日期:2004-09-30 00:00:00
abstract::Both major depression and depressive symptoms are associated with a high rate of nicotine dependence, and a history of major depression has an adverse impact on smoking cessation. The main objective of this study was to investigate whether continuous ingestion of nicotine affects indices of depressive behavior in the ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(99)00091-8
更新日期:1999-10-01 00:00:00
abstract::The present study analyzes the testosterone (T), cortisol (C) and emotional response in competitive interactions between dyads, as well as the relationship between basal T and the emotional response. Seventy-two men and women (36 dyads) participated in same-sex dyads in a face-to-face laboratory competition, and thirt...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2019.03.025
更新日期:2019-07-01 00:00:00
abstract::This study measured plasma corticosterone levels in male rats during repeated daily presentations of two intensities of restraint stress. The corticosterone response to a stress session was defined as the change from pre-stress levels to levels after 60 minutes of restraint. With the relatively intense stress imposed ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(88)90097-2
更新日期:1988-01-01 00:00:00
abstract::The use of heart rate variability (HRV) for monitoring stress has been growing in the behavioral health literature, especially in the areas of posttraumatic stress disorder, stress reactivity, and resilience. Few studies, however, have included general populations under workplace conditions. This study evaluates wheth...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2019.112734
更新日期:2020-02-01 00:00:00
abstract::Retrochiasmatic frontolateral knife cuts (FLC) or sham operations (Sham) were performed with a Halasz-type knife. All animals were primed with estrogen plus 0.5 mg progesterone (P) and tested for lordosis both before and after surgery. Two weeks after the last test they received estradiol (E2) in Silastic capsules and...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(82)90008-7
更新日期:1982-08-01 00:00:00
abstract::Winter is an energetically stressful period for small mammals as increasing demands for thermoregulation are often coupled with shortages of food supply. In sub-tropical savannah, Hottentot golden moles (Ambysomus hottentottus longiceps) forage throughout the year and for long periods of each day. This may enable them...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2005.02.022
更新日期:2005-04-13 00:00:00
abstract::Plasma noradrenaline (NA), adrenaline (A) and corticosterone (CS) concentrations were determined simultaneously in permanently heart-cannulated rats before and during the performance of reinforced and nonreinforced (extinguished) operant behavior. Shortly before the experimental food-reinforced (VI 15-sec schedule) le...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(90)90079-j
更新日期:1990-04-01 00:00:00
abstract::The adrenal cortex function of farmed blue (Alopex lagopus) and silver foxes (Vulpes vulpes) differing in their reaction in the feeding test were assessed. The urine cortisol:creatinine ratio was lower for those animals eating in the feeding test in comparison to those not eating in both species. In addition, eater si...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(98)00232-7
更新日期:1999-01-01 00:00:00
abstract::Prenatal stress (PS) induces cognitive deficits, abnormal behavior patterns and physical impairments in offspring, which disturbs the developmental process. GABA systems play a key role in the brain development. The developmental trajectories are less understood although PS increases the expression of GABAAR in young ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2020.113184
更新日期:2021-01-01 00:00:00
abstract::The influences of three methyl xanthines (MX) on human taste responsivity, caffeine, theophylline, and theobromine, were examined using blind control procedures. Taste responsivity in the same subjects was determined using the matching procedure described by Schiffman (Study 1) and the whole-mouth procedure described ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(92)90347-5
更新日期:1992-09-01 00:00:00
abstract::Binge eating is characterized by the consumption of a large amount of palatable food in a short period of time and is a core feature of many eating disorders. Patients with eating disorders are also known to display impairments in inhibitory control, cognition and decision-making, which may promote and maintain binge ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2019.03.005
更新日期:2019-07-01 00:00:00
abstract::Siberian hamsters (Phodopus sungorus) increase food hoarding, but not food intake, after a fast. Because the physiological mechanisms underlying these changes in food hoarding are virtually unknown, we sought insight into these mechanisms by allowing hamsters to self-select their diet from food sources varying in macr...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/s0031-9384(99)00167-5
更新日期:1999-12-01 00:00:00
abstract::Estradiol exerts an inhibitory effect on food intake via interactions with anorexigenic peptides, like cholecystokinin, that function to decrease meal size. It is currently unknown whether estradiol also interacts with orexigenic compounds implicated in the physiological control of food intake. Thus, the primary goal ...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2006.05.002
更新日期:2006-07-30 00:00:00
abstract::The discovery of the molecular mechanisms underlying the circadian clock, which functions in virtually every cell throughout the body to coordinate biological processes to anticipate and better adapt to daily rhythmic changes in the environment, is one of the major biomedical breakthroughs in the 20th century. Twenty ...
journal_title:Physiology & behavior
pub_type: 杂志文章,评审
doi:10.1016/j.physbeh.2018.04.017
更新日期:2018-09-01 00:00:00
abstract::Classical conditioning is a quintessential learning process; however, maladaptive forms of conditioning sustain many unhealthy behaviors (e.g., addiction). Mindfulness training is theorized to de-automatize conditioned behavior by decoupling stimulus and response. This study assessed the effect of mindfulness training...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/j.physbeh.2019.02.028
更新日期:2019-05-15 00:00:00
abstract::The effects of denervation of fungiform taste buds on preference behavior were studied. Presurgery, 0.01, 0.1, 0.2, and 0.4 M NaCl, and 0.1, 0.2, and 0.4 M KCl were strongly avoided relative to water as measured with 48-h intakes. Bilateral cuts of the chorda tympani nerve (CT) resulted in a decrease in the strength o...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::Alzheimer's disease (AD) is a neurodegenerative disorder, characterized by a progressive loss of cognitive function. Despite considerable progress, a complete description of the molecular pathology of this disease has yet to be elucidated. In this respect, the need for an animal model that develops some or all aspects...
journal_title:Physiology & behavior
pub_type: 杂志文章,评审
doi:10.1016/s0031-9384(01)00524-8
更新日期:2001-08-01 00:00:00
abstract::The taste reactivity (TR) test was devised as a method to obtain behavioural data in response to gustatory stimuli in neurologically impaired rats, incapable of voluntary feeding. Sapid solutions were infused through surgically implanted intraoral cannulae. Facial and motor responses corresponded well to known hedonic...
journal_title:Physiology & behavior
pub_type: 杂志文章
doi:10.1016/0031-9384(95)00115-y
更新日期:1995-11-01 00:00:00