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Improved aqueous extraction of microalgal lipid by combined enzymatic and thermal lysis from wet biomass of Nannochloropsis oceanica.

Abstract：

:High moisture content in wet algal biomass hinders effective performance of current lipid extraction methods. An improved aqueous extraction method combing thermal and enzymatic lysis was proposed and performed in algal slurry of Nannochloropsis oceanica (96.0% moisture) in this study. In general, cell-wall of N. oceanica was disrupted via thermal lysis and enzymatic lysis and lipid extraction was performed using aqueous surfactant solution. At the optimal conditions, high extraction efficiencies for both lipid (88.3%) and protein (62.4%) were obtained, which were significantly higher than those of traditional hexane extraction and other methods for wet algal biomass. Furthermore, an excessive extraction of polar lipid was found for wet biomass compared with dry biomass. The advantage of this method is to efficiently extract lipids from high moisture content algal biomass and avoid using organic solvent, indicating immense potential for commercial microalgae-based biofuel production.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Chen L,Li R,Ren X,Liu T

doi

10.1016/j.biortech.2016.04.031

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

138-143

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(16)30518-1

journal_volume

214

pub_type

杂志文章

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