Abstract：
:A segmented flow containing a buffer-ionic liquid/solvent in a micro-channel reactor was applied to synthesize isoquercitrin by the hesperidinase-catalyzed selective hydrolysis of rutin, based on a novel system of reaction coupling with separation. Within the developed microchannel reactor with one T-shaped inlet and outlet, the maximum isoquercitrin yield (101.7 ± 2.6%) was achieved in 20 min at 30 °C and 4 μL/min. Compared with a continuous-flow reactor, reaction rate was increased 4-fold due to a glycine-sodium hydroxide:[Bmim][BF4]/glycerol triacetate (1:1, v/v) system that formed a slug flow in microchannel and significantly increased mass transfer rates. The mass transfer coefficient significantly increased and exhibited a linear relationship with the flow rate. Hesperidinase could be efficiently reused at least 5 times, without losing any activity. The bonding mechanism and secondary structure of hesperidinase indicated that hesperidinase had a greater affinity to rutin at a production rate of 4 μL/min in this segmented flow microreactor.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Gong A,Gu SS,Wang J,Sheng S,Wu FAdoi
10.1016/j.biortech.2015.06.143subject
Has Abstractpub_date
2015-10-01 00:00:00pages
498-506eissn
0960-8524issn
1873-2976pii
S0960-8524(15)00945-1journal_volume
193pub_type
杂志文章abstract：:This study addresses in situ transesterification of highly wet microalgae with hydrochloric acid (HCl) as a catalyst. In situ transesterification was performed by heating the mixture of wet algal cells, HCl, methanol, and solvent in one pot, resulting in the fatty acid methyl ester (FAME) yield over 90% at 95°C. The e...
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更新日期：2019-06-01 00:00:00
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journal_title：Bioresource technology
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.01.020
更新日期：2008-02-01 00:00:00
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journal_title：Bioresource technology
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doi：10.1016/j.biortech.2007.01.021
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doi：10.1016/j.biortech.2012.11.042
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更新日期：2009-01-01 00:00:00
abstract：:Benzethonium chloride (BZC) is an antibacterial compound with extensive applications in various anti-infective products. However, the feasibility of attaining partial nitrification of municipal wastewater using BZC has not been reported. In this work, BZC was used for the first time to attain partial nitrification. Ba...
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abstract：:Response surface methodology was used to optimise a two-step process of cellulase mediated saccharification of rice straw by an isolated bacterium Lysinibacillus sphaericus. CMC concentration, yeast extract, pH and incubation temperature were optimised for cellulase production using a central composite design and thei...
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更新日期：2014-12-01 00:00:00
abstract：:Citric acid (CA) has been widely used in different industrial sectors, being produced through fermentation of low-cost feedstock. The development of downstream processes, easier to operate, environmentally friendly, and more economic than precipitation, is certainly a challenge in CA bioproduction. Large volumes of by...
journal_title：Bioresource technology
pub_type： 杂志文章,评审
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更新日期：2021-01-01 00:00:00
abstract：:Xylonic acid can be produced with high-yield from hemicelullosic xylose, which accounts for 25% of the total sugars in lignocellulosic material. The key barrier associated with efficient bio-oxidation of hemicellulosic xylose to xylonic acid is the serious foam formed in downstream air-aerated and agitated bioreaction...
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