Putting Two Heads Together to Build a Better Brain.

abstract：:To prevent insertional mutagenesis arising from retroviral reactivation, cells of embryonic origin possess a unique capacity to silence retroviruses. Given the distinct modes of X chromosome inactivation between embryonic and extraembryonic lineages, we investigated paradigms of viral extinction. We show that trophect...

journal_title：Cell stem cell

authors： Golding MC,Zhang L,Mann MR

更新日期：2010-05-07 00:00:00

abstract：:A survey of U.S. stem cell scientists shows that uncertainty following the legal challenge to the Obama Administration's hESC research policy has negative scientific and economic impacts and affects a range of stem cell scientists, not just those working with hESCs. The international implications of these results are ...

journal_title：Cell stem cell

authors： Levine AD

更新日期：2011-02-04 00:00:00

abstract：:The cellular identity of niche cells that regulate hematopoietic stem cell (HSC) self-renewal and differentiation has been debated for several years. Two recent studies in Nature (Ding and Morrison, 2013; Greenbaum et al., 2013) have shed light into the bone marrow stromal subsets making CXCL12, a chemokine critical f...

journal_title：Cell stem cell

authors： Hanoun M,Frenette PS

更新日期：2013-04-04 00:00:00

abstract：:The maintenance of mouse embryonic stem cells (mESCs) requires LIF and serum. However, a pluripotent "ground state," bearing resemblance to preimplantation mouse epiblasts, can be established through dual inhibition (2i) of both prodifferentiation Mek/Erk and Gsk3/Tcf3 pathways. While Gsk3 inhibition has been attribut...

journal_title：Cell stem cell

authors： Yeo JC,Jiang J,Tan ZY,Yim GR,Ng JH,Göke J,Kraus P,Liang H,Gonzales KA,Chong HC,Tan CP,Lim YS,Tan NS,Lufkin T,Ng HH

更新日期：2014-06-05 00:00:00

abstract：:Scientists are rarely immoral and seldom even amoral. The ethical principles that underlie much of their own work are shared with scientists in other countries to a much greater degree than the cultural and religious differences among those countries would lead one to expect. Like me, few will have had any formal trai...

journal_title：Cell stem cell

authors： McLaren A

更新日期：2007-06-07 00:00:00

abstract：:Although distinct human induced pluripotent stem cell (hiPSC) lines can display considerable epigenetic variation, it has been unclear whether such variability impacts their utility for disease modeling. Here, we show that although low-passage female hiPSCs retain the inactive X chromosome of the somatic cell they are...

journal_title：Cell stem cell

authors： Mekhoubad S,Bock C,de Boer AS,Kiskinis E,Meissner A,Eggan K

更新日期：2012-05-04 00:00:00

abstract：:Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and...

journal_title：Cell stem cell

authors： Czerniecki SM,Cruz NM,Harder JL,Menon R,Annis J,Otto EA,Gulieva RE,Islas LV,Kim YK,Tran LM,Martins TJ,Pippin JW,Fu H,Kretzler M,Shankland SJ,Himmelfarb J,Moon RT,Paragas N,Freedman BS

更新日期：2018-06-01 00:00:00

abstract：:Cellular reprogramming converts differentiated cells into induced pluripotent stem cells (iPSCs). However, this process is typically very inefficient, complicating mechanistic studies. We identified and molecularly characterized rare, early intermediates poised to reprogram with up to 95% efficiency, without perturbin...

journal_title：Cell stem cell

authors： Schwarz BA,Cetinbas M,Clement K,Walsh RM,Cheloufi S,Gu H,Langkabel J,Kamiya A,Schorle H,Meissner A,Sadreyev RI,Hochedlinger K

更新日期：2018-08-02 00:00:00

abstract：:Emerging evidence strongly suggests that stem cells and their differentiated progeny display distinct metabolic profiles, but how metabolic changes are coupled with organogenesis has remained unclear. Homem et al. (2014) now reveal a hormone-dependent pathway that couples metabolic changes with stem cell differentiati...

journal_title：Cell stem cell

authors： Buttitta L,Lee CY

更新日期：2014-09-04 00:00:00

abstract：:In the mouse embryo, the aorta-gonad-mesonephros (AGM) region is considered to be the sole location for intraembryonic emergence of hematopoietic stem cells (HSCs). Here we report that, in parallel to the AGM region, the E10.5-E11.5 mouse head harbors bona fide HSCs, as defined by long-term, high-level, multilineage r...

journal_title：Cell stem cell

authors： Li Z,Lan Y,He W,Chen D,Wang J,Zhou F,Wang Y,Sun H,Chen X,Xu C,Li S,Pang Y,Zhang G,Yang L,Zhu L,Fan M,Shang A,Ju Z,Luo L,Ding Y,Guo W,Yuan W,Yang X,Liu B

更新日期：2012-11-02 00:00:00

abstract：:Realizing the therapeutic potential of both stem cell and genomics research requires harnessing the respective strengths of academic, biotechnology, and pharmaceutical sectors, supported by policies and legal incentives for multisectorial collaboration. Here, we discuss issues facing these fields and lessons from the ...

journal_title：Cell stem cell

authors： Mathews DJ,Cook-Deegan R,Bubela T

更新日期：2013-05-02 00:00:00

abstract：:Regeneration of skin and hair follicles after wounding--a process known as wound-induced hair neogenesis (WIHN)--is a rare example of adult organogenesis in mammals. As such, WIHN provides a unique model system for deciphering mechanisms underlying mammalian regeneration. Here, we show that dsRNA, which is released fr...

journal_title：Cell stem cell

authors： Nelson AM,Reddy SK,Ratliff TS,Hossain MZ,Katseff AS,Zhu AS,Chang E,Resnik SR,Page C,Kim D,Whittam AJ,Miller LS,Garza LA

更新日期：2015-08-06 00:00:00

abstract：:Ectopic expression of combinations of transcription factors (TFs) can drive direct lineage conversion, thereby reprogramming a somatic cell's identity. To determine the molecular mechanisms by which Gata4, Mef2c, and Tbx5 (GMT) induce conversion from a cardiac fibroblast toward an induced cardiomyocyte, we performed c...

journal_title：Cell stem cell

authors： Stone NR,Gifford CA,Thomas R,Pratt KJB,Samse-Knapp K,Mohamed TMA,Radzinsky EM,Schricker A,Ye L,Yu P,van Bemmel JG,Ivey KN,Pollard KS,Srivastava D

更新日期：2019-07-03 00:00:00

abstract：:The re-emergence of Zika virus (ZIKV) and its suspected link with various disorders in newborns and adults led the World Health Organization to declare a global health emergency. In response, the stem cell field quickly established platforms for modeling ZIKV exposure using human pluripotent stem cell-derived neural p...

journal_title：Cell stem cell

authors： Ming GL,Tang H,Song H

更新日期：2016-12-01 00:00:00

abstract：:Stem cell-based therapies for Parkinson's disease are moving into a new and exciting era, with several groups pursuing clinical trials with pluripotent stem cell (PSC)-derived dopamine neurons. As many groups have ongoing or completed GMP-level cell manufacturing, we highlight key clinical translation considerations f...

journal_title：Cell stem cell

authors： Barker RA,Parmar M,Studer L,Takahashi J

更新日期：2017-11-02 00:00:00

abstract：:Cancer stem cells (CSCs) are tumor cells that have the principal properties of self-renewal, clonal tumor initiation capacity, and clonal long-term repopulation potential. CSCs reside in niches, which are anatomically distinct regions within the tumor microenvironment. These niches maintain the principle properties of...

journal_title：Cell stem cell

authors： Plaks V,Kong N,Werb Z

更新日期：2015-03-05 00:00:00

abstract：:The generation of distinct hematopoietic cell types, including tissue-resident immune cells, distinguishes fetal from adult hematopoiesis. However, the mechanisms underlying differential cell production to generate a layered immune system during hematopoietic development are unclear. Using an irreversible lineage-trac...

journal_title：Cell stem cell

authors： Beaudin AE,Boyer SW,Perez-Cunningham J,Hernandez GE,Derderian SC,Jujjavarapu C,Aaserude E,MacKenzie T,Forsberg EC

更新日期：2016-12-01 00:00:00

abstract：:Accessibility of human oocytes for research poses a serious ethical challenge to society. This fact categorically holds true when pursuing some of the most promising areas of research, such as somatic cell nuclear transfer and embryonic stem cell studies. One approach to overcoming this limitation is to use an oocyte ...

journal_title：Cell stem cell

authors： Beyhan Z,Iager AE,Cibelli JB

更新日期：2007-11-01 00:00:00

abstract：:Newborn neurons in the adult dentate gyrus pass through several distinct precursor and progenitor classes prior to differentiation. In this issue of Cell Stem Cell, Lugert et al. (2010) characterized their strikingly different proliferative behaviors after neurogenic stimuli or aging. ...

journal_title：Cell stem cell

authors： Breunig JJ,Rakic P

更新日期：2010-05-07 00:00:00

abstract：:The identification of tumor-initiating cells represents a significant challenge for studies of prostate cancer. In a recent issue of Cell Reports, Agarwal and colleagues use organoid culture to ascertain two distinct luminal progenitors in a mouse model of prostate cancer, shedding new light on lineage relationships i...

journal_title：Cell stem cell

authors： Shen MM

更新日期：2015-12-03 00:00:00

abstract：:Cell cycle regulators play critical roles in the balance between hematopoietic stem cell (HSC) dormancy and proliferation. In this study, we report that cell cycle entry proceeded normally in HSCs null for cyclin-dependent kinase (CDK) inhibitor p57 due to compensatory upregulation of p27. HSCs null for both p57 and p...

journal_title：Cell stem cell

authors： Zou P,Yoshihara H,Hosokawa K,Tai I,Shinmyozu K,Tsukahara F,Maru Y,Nakayama K,Nakayama KI,Suda T

更新日期：2011-09-02 00:00:00

abstract：:Chronic liver injury can cause cirrhosis and impaired liver regeneration, impairing organ function. Adult livers can regenerate in response to parenchymal insults, and multiple cellular sources have been reported to contribute to this response. In this study, we modeled human chronic liver injuries, in which such resp...

journal_title：Cell stem cell

authors： Deng X,Zhang X,Li W,Feng RX,Li L,Yi GR,Zhang XN,Yin C,Yu HY,Zhang JP,Lu B,Hui L,Xie WF

更新日期：2018-07-05 00:00:00

abstract：:Mouse embryonic stem cells (mESCs) cultured under serum/LIF conditions exhibit heterogeneous expression of pluripotency-associated factors that can be overcome by two inhibitors (2i) of the MEK and GSK3 pathways. Several studies have shown that the "ground state" induced by 2i is characterized by global hypomethylatio...

journal_title：Cell stem cell

authors： Galonska C,Ziller MJ,Karnik R,Meissner A

更新日期：2015-10-01 00:00:00

abstract：:Neural precursor cells (NPCs) reside in the subventricular zone in association with blood vessels and ependymal cells. In this issue of Cell Stem Cell, Kokovay et al. (2010) show that SDF1 directs the association of NPCs with this niche and regulates their lineage progression in a stage-specific manner. ...

journal_title：Cell stem cell

authors： Siegenthaler JA,Pleasure SJ

更新日期：2010-08-06 00:00:00

abstract：:Animal cloning can be achieved through somatic cell nuclear transfer (SCNT), although the live birth rate is relatively low. Recent studies have identified H3K9me3 in donor cells and abnormal Xist activation as epigenetic barriers that impede SCNT. Here we overcome these barriers using a combination of Xist knockout d...

journal_title：Cell stem cell

authors： Matoba S,Wang H,Jiang L,Lu F,Iwabuchi KA,Wu X,Inoue K,Yang L,Press W,Lee JT,Ogura A,Shen L,Zhang Y

更新日期：2018-09-06 00:00:00

abstract：:To date, pluripotent epiblast stem cells (EpiSCs) had only been derived from postimplantation mouse embryos. In this issue of Cell Stem Cell, Najm et al. (2011) demonstrate that EpiSCs can be routinely derived from preimplantation embryos, showing that both human and mouse blastocysts can produce the same class of pri...

journal_title：Cell stem cell

authors： Kunath T

更新日期：2011-03-04 00:00:00

abstract：:Combination of stem cell-based approaches with gene-editing technologies represents an attractive strategy for studying human disease and developing therapies. However, gene-editing methodologies described to date for human cells suffer from technical limitations including limited target gene size, low targeting effic...

journal_title：Cell stem cell

authors： Liu GH,Suzuki K,Qu J,Sancho-Martinez I,Yi F,Li M,Kumar S,Nivet E,Kim J,Soligalla RD,Dubova I,Goebl A,Plongthongkum N,Fung HL,Zhang K,Loring JF,Laurent LC,Izpisua Belmonte JC

更新日期：2011-06-03 00:00:00

abstract：:Secreted frizzled-related protein 1 (Sfrp1) is highly expressed by stromal cells maintaining hematopoietic stem cells (HSCs). Sfrp1 loss in stromal cells increases production of hematopoietic progenitors, and in knockout mice, dysregulates hemostasis and increases Flk2- Cd34- Lin- Sca1+ Kit+ (LSK) cell numbers in bone...

journal_title：Cell stem cell

authors： Renström J,Istvanffy R,Gauthier K,Shimono A,Mages J,Jardon-Alvarez A,Kröger M,Schiemann M,Busch DH,Esposito I,Lang R,Peschel C,Oostendorp RA

更新日期：2009-08-07 00:00:00

abstract：:Technological limitations have hampered understanding of how individual molecules, including putative stem cell regulators, are distributed throughout tissues and stem cell niches. Here, we report adaptation of the proximity ligation assay (PLA) for large-volume, in situ imaging of individual proteins with multiple ad...

journal_title：Cell stem cell

authors： Kunz L,Schroeder T

更新日期：2019-12-05 00:00:00

abstract：:Dysfunction and loss of retinal pigment epithelium (RPE) leads to degeneration of photoreceptors in age-related macular degeneration and subtypes of retinitis pigmentosa. Human embryonic stem cells (hESCs) may serve as an unlimited source of RPE cells for transplantation in these blinding conditions. Here we show the ...

journal_title：Cell stem cell

authors： Idelson M,Alper R,Obolensky A,Ben-Shushan E,Hemo I,Yachimovich-Cohen N,Khaner H,Smith Y,Wiser O,Gropp M,Cohen MA,Even-Ram S,Berman-Zaken Y,Matzrafi L,Rechavi G,Banin E,Reubinoff B

更新日期：2009-10-02 00:00:00