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The ACBP gene family in Rhodnius prolixus: Expression, characterization and function of RpACBP-1.

Abstract：

:The acyl-CoA-binding proteins (ACBP) constitute a family of conserved proteins that bind acyl-CoA with high affinity and protect it from hydrolysis. Thus, ACBPs may have essential roles in basal cellular lipid metabolism. The genome of the insect Rhodnius prolixus encodes five ACBP genes similar to those described for other insect species. The qPCR analysis revealed that these genes have characteristic expression profiles in insect organs, suggesting that they have specific roles in insect physiology. Recombinant RpACBP-1 was able to bind acyl-CoA in an in vitro gel-shift assay. Moreover, heterologous RpACBP-1 expression in acb1Δ mutant yeast rescued the multi-lobed vacuole phenotype, indicating that RpACBP-1 acts as a bona fide acyl-CoA-binding protein. RpACBP-1 knockdown using RNAi caused triacylglycerol accumulation in the insect posterior midgut and a reduction in the number of deposited eggs. The amount of stored triacylglycerol was reduced in flight muscle, and the incorporation of fatty acids in cholesteryl esters was increased in the fat body. These results showed that RpACBP-1 participates in several lipid metabolism steps in R. prolixus.

journal_name

Insect Biochem Mol Biol

journal_title

Insect biochemistry and molecular biology

authors

Majerowicz D,Hannibal-Bach HK,Castro RSC,Bozaquel-Morais BL,Alves-Bezerra M,Grillo LAM,Masuda CA,Færgeman NJ,Knudsen J,Gondim KC

doi

10.1016/j.ibmb.2016.03.002

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

41-52

eissn

0965-1748

issn

1879-0240

pii

S0965-1748(16)30022-4

journal_volume

72

pub_type

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