Abstract：
:A binary mosquitocidal toxin composed of a three-domain Cry-like toxin (Cry48Aa) and a binary-like toxin (Cry49Aa) was identified in Lysinibacillus sphaericus. Cry48Aa/Cry49Aa has action on Culex quinquefasciatus larvae, in particular, to those that are resistant to the Bin Binary toxin, which is the major insecticidal factor from L. sphaericus-based biolarvicides, indicating that Cry48Aa/Cry49Aa interacts with distinct target sites in the midgut and can overcome Bin toxin resistance. This study aimed to identify Cry48Aa/Cry49Aa ligands in C. quinquefasciatus midgut through binding assays and mass spectrometry. Several proteins, mostly from 50 to 120 kDa, bound to the Cry48Aa/Cry49Aa toxin were revealed by toxin overlay and pull-down assays. These proteins were identified against the C. quinquefasciatus genome and after analysis a set of 49 proteins were selected which includes midgut bound proteins such as aminopeptidases, amylases, alkaline phosphatases in addition to molecules from other classes that can be potentially involved in this toxin's mode of action. Among these, some proteins are orthologs of Cry receptors previously identified in mosquito larvae, as candidate receptors for Cry48Aa/Cry49Aa toxin. Further investigation is needed to evaluate the specificity of their interactions and their possible role as receptors.
journal_name
Insect Biochem Mol Bioljournal_title
Insect biochemistry and molecular biologyauthors
Rezende TMT,Romão TP,Batista M,Berry C,Adang MJ,Silva-Filha MHNLdoi
10.1016/j.ibmb.2017.08.001subject
Has Abstractpub_date
2017-09-01 00:00:00pages
63-70eissn
0965-1748issn
1879-0240pii
S0965-1748(17)30117-0journal_volume
88pub_type
杂志文章abstract：:Neuropeptides are important controlling agents in animal physiology. In order to understand their role and the ways in which neuropeptides behave and interact with one another, information on their time and sites of expression is required. We here used a combination of MALDI-TOF and ESI-Q-TOF mass spectrometry to make...
journal_title：Insect biochemistry and molecular biology
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2015-04-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
doi：10.1016/0965-1748(94)00095-y
更新日期：1995-05-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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journal_title：Insect biochemistry and molecular biology
pub_type： 杂志文章
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